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All Journal Prosiding Seminar Nasional MIPA AL KAUNIYAH AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA BIOEDUSCIENCE
Andi Salamah
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Materials Science & Nanotechnology

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Journal : BERITA BIOLOGI

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VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
Co-Authors Abinawanto Abinawanto Achmad Dinoto DIAN HENDRAYANTI Dwiranti, Astari Endang Sukara Fadilah, Nurrahmi Iriani, Nur Annisa Khoiriyah, Istatik Nur Laili Pipit Marianingsih Rahmadina, Amara Sarjiya Antonius Sulistiani Sulistiani, Sulistiani Tri Muji Ermayanti WIBOWO MANGUNWARDOYO Yuki Ichinose Yuniati, Ratna