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Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18.26 KB)

Abstract Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus

Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan.Â Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus

Pathogenicity of Entomophatogenic Fungi Lecanicillium lecanii Against Predator Insect Menochilus Sexmaculatus Hadi, Mochammad Syamsul; Taufiqurrahman, Achmad Fitriadi; Choliq, Fery Abdul; Istiqomah, Istiqomah; Karindah, Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.115.63-68

Lecanicillium lecanii is an insect pathogenic fungus that is often used for pest control and has a wide range of hosts. The L. lecanii is capable of infecting several types of host insects including the Order Orthoptera, Hemiptera, Lepidoptera, Thysanoptera and Coleoptera. The extent of this fungus host range was feared to have a negative effect on predator insects Menochilus sexmaculatus. This study aims were to determined the pathogenicity of the fungus L. lecanii against the imago predatory beetle M. sexmaculatus, to know how the predation ability and the number of eggs fecundity of Imago M. sexmaculatus after application L. lecanii. The research was arranged in Randomized Block Design with 4 replications. The conidia density of L. lecanii used were 106, 107, 108, 109 conidia/ml, 1 ml / l of lufenuron insecticide as positive control and sterile distilled water as negative control. The research showed that the mortality percentage of M. sexmaculatus due to L. lecanii application is low and medium. The L. lecanii was not affected for preying ability on imago M. sexmaculatus but gave effect to the number of eggs fecundity of imago M. sexmaculatus.

Pathogenicity of Entomophatogenic Fungi Lecanicillium lecanii Against Predator Insect Menochilus Sexmaculatus Hadi, Mochammad Syamsul; Taufiqurrahman, Achmad Fitriadi; Choliq, Fery Abdul; Istiqomah, Istiqomah; Karindah, Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 2 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.115.63-68

Lecanicillium lecanii is an insect pathogenic fungus that is often used for pest control and has a wide range of hosts. The L. lecanii is capable of infecting several types of host insects including the Order Orthoptera, Hemiptera, Lepidoptera, Thysanoptera and Coleoptera. The extent of this fungus host range was feared to have a negative effect on predator insects Menochilus sexmaculatus. This study aims were to determined the pathogenicity of the fungus L. lecanii against the imago predatory beetle M. sexmaculatus, to know how the predation ability and the number of eggs fecundity of Imago M. sexmaculatus after application L. lecanii. The research was arranged in Randomized Block Design with 4 replications. The conidia density of L. lecanii used were 106, 107, 108, 109 conidia/ml, 1 ml / l of lufenuron insecticide as positive control and sterile distilled water as negative control. The research showed that the mortality percentage of M. sexmaculatus due to L. lecanii application is low and medium. The L. lecanii was not affected for preying ability on imago M. sexmaculatus but gave effect to the number of eggs fecundity of imago M. sexmaculatus.

POTENCY OF BLACK TEA FILTRATE AS UV PROTECTANT TO S/NPV JTM 97 C IN CONTROLLING HELICOVERPA ARMIGERA ON SOYBEAN Anella Retna Kumala Sari; Tiara Eka Ariestantia; Fery Abdul Choliq
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.40-48

Helicoverpa armigera is the important pest on soybean which usually controlled by chemical insecticide spraying, so it should be replaced by naturally control using biological agents such as Spodoptera litura Nuclear Polyhedrosis Virus (S/NPV) JTM 97 C. It has some weakness such as during it’s applied in the field the effectiveness can become decreased after exposure the sunlight, especially ultraviolet (UV) so it needs the protective material, such as black tea filtrate which is known containing epigallocatechin gallate, caffeic acid, and apigenin which have role as UV protectors. This research aimed to investigate the potential of black tea filtrate to protect the S/NPV JTM 97 C from UV in controlling of H. armigera. The research was conducted in Pests and Diseases Laboratory on Indonesian Legumes and Tuber Crops Research Institute (ILETRI), Kendalpayak, Malang from January to June 2015. The research used Completely Randomized Design with seven treatments and four replications. They were S/NPV JTM 97 C without UV protectant as control (P1), S/NPV JTM 97 C + kaolin as comparison (P2), S/NPV JTM 97 C + black tea filtrate concentration 0.5 (P3), 1.0 (P4), 1.5 (P5), 2 (P6), and 2.5% (P7). Results showed that all of the protective materials of black tea filtrate were unable to provide mortality of H. armigera larvae better than kaolin. Kaolin can cause mortality of H. armigera larvae up to 82,50%. All of Black tea filtrate concentrations tested were not able to be used as protectant of S/NPV JTM 97 C due to it can only cause mortality of H. armigera up to 47,50% . Keywords: Entomopathogenic virus, LC50, protectant material, pest.

Antifungal Effects of Plant Extracts on Colletotrichum gloeosporides in Chilli Pepper (Capsicum frutescens L.) Awanda Zidni Rizki; Fery Abdul Choliq; Mintarto Martosudiro
Journal of Tropical Plant Protection Vol 2, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.5

Chili pepper (Capsicum frutescens L.) is a horticultural commodity that has high economic value and has the potential as an export commodity. One of the factors causing the decrease in productivity of chilli pepper is anthracnose caused by C. gloeosporioides. Consequently, plant-based fungicides might be served as eco-friendly alternatives to control C. gloeosporioides. This study aimed to invesitigated the effect of plant extracts from clove flowers, fennel seeds, basil, sweet basil, and citronella on growth inhibition of C. gloeosporioides in-vitro and in-vivo. Data were analyzed using Analysis of Variance (ANOVA). The five plant extracts were able to inhibit the growth of the pathogen C. gloeosporioides in vitro and in vivo. The highest inhibition of the pathogen C. gloeosporioides was basil extract treatment in-vitro compared to the control treatment. The in-vivo treatment, the lowest growth of C. gloeosporioides in fennel extract and was significanly different compared to the control treatment.

Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants Nafisatul Afidah; Tutung Hadiastono; Bedjo Bedjo; Fery Abdul Choliq
Journal of Tropical Plant Protection Vol 2, No 1 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.1.2

Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.

Growth of Beauveria bassiana combined with MIPC Insecticide and its efficacy to control the brown planthopper Nilaparvata lugens Aminudin Afandhi; Yayan Atma Kunjana; Fery Abdul Choliq; Amin Setyo Leksono
Research Journal of Life Science Vol 7, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.01.2

The objectives of this study were to evaluate the efficacy of MIPC to control the brown planthopper Nilaparvata lugens, one of the most damaging pests of rice in the field and determine the effect of various of its doses on the growth of the fungus B. bassiana. The research was carried out in a rice field and in the Biological Agents Development Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University, Indonesia. The field efficacy test of the MIPC insecticide was conducted by applying 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses. The insecticidal compatibility test of MIPC with B. bassiana was conducted in 0.5-, 1.0-, 1.5-, and 2.0-g/L doses of MIPC. Application of MIPC insecticide at 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses had no significant difference in reducing N. lugens population in the vegetative phase (28 to 70 days after planting). The MIPC doses of 1.0, 1.5, and 2.0 g/L were highly toxic or incompatible with the growth of B. bassiana. Meanwhile, a sublethal concentration of MIPC insecticide of 0.5 g/L was non-toxic to B. bassiana. Therefore, this study recommends the use of 0.5 g/L MIPC combined with B. bassiana fungi.

Distribution of the Endophytic Fungi in Apple Leaves Aminudin Afandhi; Fery Abdul Choliq; Havinda Anggrilika W.S.; Hagus Tarno
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1563

Endophytic fungi have roles to protect the plant from herbivorous insects and diseases, and to support the absorption process of nutrients needed by plants for photosynthesis. Related to the important role of endophytic fungi, research was aimed to identify fungal endophytes associated with young, mature and old leaves on apple and to evaluate the effect of leaf ages on the abundance and diversity of endophytic fungi. The research was conducted in Biological Control Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya from May to September 2016. Apple leaves sampling was done diagonally, and taken from nine apple trees. Based on macroscopic and microscopic characteristics, 38 isolates were identified. In the old apple leaves, it obtained 17 isolates that consisted of 5 genera, mature leaves obtained 14 isolates consisted of 6 genera, and young leaves 7 isolates obtained consists of 2 genera. The mature leaves have highest abundance and diversity of endophytic fungi compared to young and old leaves. Endophytic fungi apple leaf dominated by Aspergillus. Mature and young leaves were different from old one based on Bray-Curtis similarity.

Identifikasi Penyakit yang Disebabkan oleh Virus pada Tanaman Pepaya (Carica papaya L.) di Malang, Jawa Timur Fery Abdul Choliq; Tutung Hadi Astono; Istiqomah Istiqomah; Miladiyatul Fauziyah
Gontor AGROTECH Science Journal Vol 4, No 2 (2018): December 2018
Publisher : University of Darussalam Gontor, Ponorogo, East Java Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/agrotech.v4i2.2442

Abstract : Papaya ringspot merupakan penyakit baru pada tanaman pepaya di Indonesia yang disebabkan oleh patogen Papaya ringspot virus (PRSV). Tujuan dari penelitian ini yaitu untuk mengetahui virus penyebab penyakit pada tanaman pepaya di Malang. Penelitian ini dilaksanakan di Laboratorium Virologi, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya dan rumah kaca di Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang. Metode penelitian yang digunakan yaitu metode deskriptif untuk mengetahui jenis virus pada tanaman pepaya. Hasil penelitian menunjukkan gejala pada tanaman pepaya di lapang yaitu mosaik dan klorosis pada lamina daun serta adanya bercak cincin atau ringspot pada permukaan buah. Hasil penularan pada tanaman indikator Chenopodium amaranticolor dan Chenopodium quinoa menghasilkan gejala lesio lokal nekrosis. Berdasarkan hasil identifikasi menggunakan TEM morfologi partikel virus tergolong kelompok Potyvirus, berbentuk filament flexuous dengan ukuran sekitar 800-900 nm x 12 nm. Virus yang diuji mempunyai kisaran inang tanaman dari famili Caricaceae dan dua jenis tanaman dari famili Cucurbitaceae yaitu Cucumis sativus dan Cucumis melo L. Berdasarkan hasil pengujian sifat fisik virus diketahui nilai Dilution End-Point (DEP), Thermal Inactivation Point (TIP), dan Longevity In Vitro (LIV) virus yang diuji yaitu 10-5-10-6, 65oC, dan 24-48 jam pada suhu ruang. Berdasarkan nilai tersebut dapat disimpulkan virus yang menginfeksi tanaman pepaya di Malang termasuk dalam famili Potyvirus yaitu Papaya ringspot virus (PRSV).

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