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All Journal JKPK (Jurnal Kimia dan Pendidikan Kimia)
M. Suzery
Department of Chemistry, Faculty of Sciences and Mathematics, Universitas Diponegoro, Jl. Prof. Soedarto No.50275, Tembalang, Semarang City, Central Java, 50275
Chemical Engineering, Chemistry & Bioengineering Chemistry

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The Comparison of Spectrophotometric and TLC-Densitometric for DPPH Radical Scavenging Activity Analysis of Three Medicinal Plant Extracts Bambang Cahyono; Christina Setyadewi M.; M. Suzery; Agustina L. N. Aminin
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 5, No 2 (2020): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v5i2.40370

Abstract

In this research, Thin Layer Chromatography-Densitometry has proven to be a good method for analyzing 1, 1 diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity, since this approach displayed the similar trends with UV-Vis spectrophotometric method. Three medicinal plants collected from Semarang used to evaluate both methods.  The IC50 value ranged from 25.26 - 4913.74 ppm shown by UV-Vis spectrophotometric and 24.74 – 4674.61 ppm using TLC-Densitometric. Meniran Dechlorophyllated (Phyllanthus niruri) provides the strongest antioxidant activity and the weakest de-chlorophylated parsley (Petroselinum crispum). The paired sample t-test points from a non-dechlorophylation extract using the TLC densitometry test significantly gives a lower IC50 value than the UV spectrophotometry method-has. The maximum increase of peak area under the UV light 365 nm up to 56.08 %. This evidence supports the presumption that the scavenging radical DPPH caused not only decreasing the maximum absorbance under UV light 516 nm but also increasing the absorbance under UV light ± 365 nm.
Co-Authors Agustina L.N. Aminin Bambang Cahyono Christina Setyadewi M.