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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Magister Pendidikan IPA
Dwi Soelistya Dyah Jekti
Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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BAKTERI ENDOFIT KULIT BATANG TANAMAN CENGKEH (Syzygium aromaticum L.) DAN KEMAMPUANNYA SEBAGAI ANTIBAKTERI Citra Sepriana; Dwi Soelistya Dyah Jekti; Lalu Zulkifli
Jurnal Penelitian Pendidikan IPA Vol. 3 No. 2 (2017): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v3i2.92

Abstract

This research was conducted to find out the capabilities of endophytic bacteria isolated from bark of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria, antibacterial test, biochemical test, and molecular identification based on 16 S rRNA. It was obtained about 29 isolates from bark of the cloves plants in which 6 of them isolates inhibited bacterial S. aureus and 3 isolates inhibited bacterial S. aureus and S. mutans. Based on 16S rRNA molecular identification, isolates of endophytic bacterial of cloves plants bark which have inhibitory is closely related to Bacillus cereus JL and Bacillus amyloliquefasiensKeywords: Bark of the cloves, Endophytic bacterial, Molecular  identification
ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI ENDOFIT KULIT BATANG SRIKAYA (ANNONA SQUAMOSA) DAN POTENSINYA SEBAGAI ANTIBAKTERI Lalu Zulkifli; Dwi Soelistya Dyah Jekti; Samsul Bahri
Jurnal Penelitian Pendidikan IPA Vol. 4 No. 1 (2018): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v4i1.98

Abstract

The objective of this study was to isolate endophytic bacteria from bark of srikaya, analyzing antibacterial activity of endophytic bacteria in S. aureus, B. cereus and E. coli pathogenic bacteria, characterizing endophytic bacteria capable of inhibiting the growth of pathogenic bacteria and identification of endophytic bacteria able to inhibit the growth of pathogenic bacteria. Isolation of endophytic bacteria using TSA and NA media, bioassay on pathogen bacteria with concentration of 106 cells / ml with using Ø 6 mm wells and entering supernatant of 100 μl. Supernatant was obtained by growing endophytic bacteria in NB media shaken with shaker 150 cycles / min for 48 h at 32ᵒC then culture centrifuge at 5000 g for 30 min. Positive control using cyprofloxacin. Characterization is based on the nature of the colony, Gram paint, spore formation, and biochemical tests. The results of the study yielded 13 endophytic bacterial isolates and 4 endophytic isolates capable of inhibiting the growth of 8 pathogenic bacteria with sensitive criteria, 2 pathogenic bacteria with resistant criteria and 1 pathogen bacteria can not be inhibited its growth. Gram's paint results show that 4 endophytic isolates belong to Gram-positive, rod-shaped and spore forming cells. From the character possessed by the bacteria can be identified that the 4 bacteria endofit capable of inhibiting pathogenic bacteria are Bacillus brevis, Bacillus latesporus, Virgibacillus pantothenticus, and Bacillus circulansKeywords: Characterization, Endophytic Bacteria, Bark of Srikaya, Antibacterial
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH JERUK NIPIS (Citrus aurantifolia Swingle) TERHADAP PERTUMBUHAN BAKTERI ISOLAT KLINIS Rohmi Wardani; Dwi Soelistya Dyah Jekti; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 5 No. 1 (2019): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v5i1.101

Abstract

The objective of this research was to test antibacterial activity ethanol and ethyl acetate of lime peel (Citrus aurantifolia Swingle) extract by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) against the growth of clinical isolate bacteria. The type of this study used laboratory experimental using sensitivity test, dilution of tube and spreading plate. The experimental design used Complete Random Design using three repetitions on three concentrations i.g 25, 50, and 75%. Data was analyzed by qualitative with measuring inhibit diameter zone bacterial and qualitative using Two Way Anova followed by BNT with α= 5%. The statistical analysis showed that the solvent factor and concentration factor was significantly against growing bacterial and there was an interaction affect of both factors on S. aureus growth. The result presented that the ethanol and ethyl acetate of lime peel extract strongly inhibited Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa; and midly inhibited the growth of Klebsiella pneumoniae. The MIC ethanol and ethyl acetate of lime peel extract against S. aureus, S. epidermidis and P. aeruginosa was 25% and K. pneumoniae was 50%. The MBC showed that ethanol and ethyl acetate of lime peel  extract killed bacteria P. aeruginosa at 15%, S. aureus, S. epidermidis at 20% and K. pneumoniae at 25%. Taken together, it can be concluded that ethyl acetate of lime extract more effective than that of ethanol extract was to inhibit and killed clinical isolate bacterial.Keywords: lime peel (Citrus aurantifolia Swingle), antibacterial activity, clinical isolate bacteria, MIC and MBC.
Perangkat Pembelajaran IPA Model 5E : Kajian Kritis Hasil Workshop Guru-Guru Anggota MGMP IPA Kota Mataram Dwi Soelistya Dyah Jekti; Imam Bachtiar; Prapti Sedijani; Lalu Zulkifli; Dadi Setiadi
Jurnal Pengabdian Magister Pendidikan IPA Vol 1 No 1 (2018)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.233 KB) | DOI: 10.29303/jpmpi.v1i1.205

Abstract

Abstrak: Kurikulum Mata Pelajaran IPA SMP tahun 2017 di Kota Mataram  menuntut para guru IPA untuk bisa meningkatkan keterampilan dalam mengembangkan perencanaan  dan mengimplementasikannya secara baik. Salah satunya adalah guru IPA SMP bisa mengembangkan perangkat pembelajaran. Untuk dapat mengembangkan perangkat pembelajaran IPA sesuai dengan standar,  para guru IPA harus memiliki keterampilan dalam mengembangkan perangkat pembelajaran, Para guru masih terpaku dengan meodel yang disarankan kemnetrian terkait, sementara  banyak model pembelajaran berbasisi siswa aktif salah satunya model 5E (Engage, Explore, Explain, Extend dan Evaluate).  Oleh karena itu perlu dilakukan kegiatan untuk mengmbangkan keterampilan  bapak/ibu guru IPA anggota MGMP IPA dalam perangkat pembelajaran model 5E. Desain yang digunakan adalah post tes no control group design. Populasi adalah semua guru IPA anggota MGMP Kota Mataram, sampel yang digunakan sebanyak 40 guru. Pengumpulan data dilakukan dengan mengevaluasi produk berupa perangkat pembelajaran hasil kegiatan workshop. Data dianalisis deskriptif kuantitatif. Hasil menunjukan bahwa perangkat pembelajaran yang disusun oleh guru peserta (90%) sudah baik dalam merencanakan langkah-langkah 5E. Tetapi dalam kaitannya dengan tagihan dan pengetahuan sebagian besar perangkat pembelajaran kurang mencakup keluasan dan kedalaman materi yang ditagih oleh kompetensi dasar pengetahuan dan keterampilan. Dengan demikian walaupun langkah-langkah pembelajaran berbasis siswa aktif dan memenuhi tagihan kurikulum tetapi tingkat asesmen belum memenuhi tagihan. Berdasarkan hasil penjelasan di atas dapat disimpulkan bahwa  90% bapak ibu guru IPA dapat menyusun perangkat pembelajaran cukup baik khususnya terkait sintaks  5E. Kata Kunci : pembelajaran IPA, model 5E, kajian kritis
Co-Authors Dadi Setiadi Imam Bachtiar Lalu Zulkifli Prapti Sedijani Prapti Sedijani Rohmi Wardani Samsul Bahri Sepriana, Citra