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All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Farmasi Klinik Indonesia JURNAL FARMASI GALENIKA
Marlia Singgih
School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung, Indonesia
Computer Science & IT Education Health Professions Medicine & Pharmacology

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Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients Singgih, Marlia; Ahsogan, Kanaka L.; Puspitasari, Irma M.; Damayanti, Sophi
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.595 KB) | DOI: 10.15416/ijcp.2017.6.1.38

Abstract

Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known asa diet food that is recommended for diabetic and coronary heart disease patients. A relatively shortershelf life of paneer is considered to be a major hurdle in its production. The present preliminary studywas conducted to determine antibacterial activity of Lactobacillus plantarum against Eschericia coliand Bacillus cereus, to develop it as biopreservative agent in paneer before using it for dietary food ofdiabetic and coronary heart disease patients in next clinical study. The antibacterial activities were testedthrough Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)using microdilution well method. Biopreservative activity in paneer was tested using total plate countmethod based on time and temperature variations. Result showed that Lactobacillus plantarum inhibitedBacillus cereus and Eschericia coli with MIC of 3125 and 1562.5 μg/mL whereas MBC was in a value of>6250 and >3125 μg/mL, respectively. As a biopreservative agent, addition of Lactobacillus plantarumto paneer showed no bacterial growth until 7 days in room temperature and 9th day in cold temperature. Itis concluded that Lactobacillus plantarum could be used as a natural biopreservative agent for extendingthe shelf life of paneer. The paneer with addition of Lactobacillus plantarum as biopreservative will thenbe consumed by diabetic patient and coronary heart patients in next clinical study.
PEMBENTUKAN ZAT WARNA Monascus purpureus DENGAN LIMBAH AMPAS KELAPA SEBAGAI SUBSTRAT DAN UJI AKTIVITASNYA TERHADAP Escherichia coli dan Candida albicans Singgih, Marlia; Julianti, Elin; Yuliana, Anna; Singgih, Marlia
JURNAL FARMASI GALENIKA Vol 5 No 2 (2018): Jurnal Farmasi Galenika Volume 5 No. 2, 2018
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.513 KB)

Abstract

Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan dua variasi sampel limbah ampas kelapa sebagai substrat. Dilakukan pengukuran pembentukan zat warna dengan cara mengekstraksi sampel menggunakan etanol 95% pada fermentasi hari ke-7 dan ke-14 dilanjutkan dengan pengujian kromatografi lapis tipis menggunakan pengembang etanol:etilasetat (7:3). Serapan zat warna diukur dengan spektrofotometer UV-Vis pada panjang gelombang 400, 406, 498, 500, 511 dan 512 nm. Hasil menunjukkan serapan zat warna mengalami peningkatan dengan rentang serapan antara 0,128–0,269 dan 0,212-1,019 dan Sampel A menunjukkan serapan lebih tinggi dibandingkan Sampel B. Pengujian aktivitas antimikroba ekstrak zat warna ke dua sampel dilakukan terhadap Escherichia coli dan Candida albicans menunjukkan adanya aktivitas antimikroba. Kesetaraan aktivitas berdasarkan kurva baku Tetrasiklin HCl terhadap Escherichia coli dan kurva baku Nistatin terhadap Candida albicans menunjukkan ekstrak zat warna Monascus purpureus dengan konsentrasi 100% pada Sampel A lebih besar dibandingkan Sampel B. Dengan dua variasi waktu yang berbeda hari ke-7 dan hari ke-14 Sampel A mempunyai aktivitas kesetaraan konsentrasi 1,41% dan 4,13% Tetrasiklin HCl serta 0,81% dan 1,38% Nistatin. Sampel B pada hari ke-7 dan hari ke-14 mempunyai aktivitas kesetaraan konsentrasi 1,02% dan 2,44% Tetrasiklin HCl serta 0,79% dan 1,20% Nistatin.
PEMBENTUKAN ZAT WARNA Monascus purpureus DENGAN LIMBAH AMPAS KELAPA SEBAGAI SUBSTRAT DAN UJI AKTIVITASNYA TERHADAP Escherichia coli dan Candida albicans Singgih, Marlia; Julianti, Elin; Yuliana, Anna; Singgih, Marlia
JURNAL FARMASI GALENIKA Vol 5 No 2 (2018): Jurnal Farmasi Galenika Volume 5 No. 2, 2018
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.513 KB)

Abstract

Telah dilakukan penelitian pembentukan zat warna Monascus purpureus hasil fermentasi padat dengan dua variasi sampel limbah ampas kelapa sebagai substrat. Dilakukan pengukuran pembentukan zat warna dengan cara mengekstraksi sampel menggunakan etanol 95% pada fermentasi hari ke-7 dan ke-14 dilanjutkan dengan pengujian kromatografi lapis tipis menggunakan pengembang etanol:etilasetat (7:3). Serapan zat warna diukur dengan spektrofotometer UV-Vis pada panjang gelombang 400, 406, 498, 500, 511 dan 512 nm. Hasil menunjukkan serapan zat warna mengalami peningkatan dengan rentang serapan antara 0,128–0,269 dan 0,212-1,019 dan Sampel A menunjukkan serapan lebih tinggi dibandingkan Sampel B. Pengujian aktivitas antimikroba ekstrak zat warna ke dua sampel dilakukan terhadap Escherichia coli dan Candida albicans menunjukkan adanya aktivitas antimikroba. Kesetaraan aktivitas berdasarkan kurva baku Tetrasiklin HCl terhadap Escherichia coli dan kurva baku Nistatin terhadap Candida albicans menunjukkan ekstrak zat warna Monascus purpureus dengan konsentrasi 100% pada Sampel A lebih besar dibandingkan Sampel B. Dengan dua variasi waktu yang berbeda hari ke-7 dan hari ke-14 Sampel A mempunyai aktivitas kesetaraan konsentrasi 1,41% dan 4,13% Tetrasiklin HCl serta 0,81% dan 1,38% Nistatin. Sampel B pada hari ke-7 dan hari ke-14 mempunyai aktivitas kesetaraan konsentrasi 1,02% dan 2,44% Tetrasiklin HCl serta 0,79% dan 1,20% Nistatin.
TRANSFORMASI PLASMID PTRLI DENGAN TEKNIK ELEKTROPORASI PADA ASPERGILLUS TERREUS DAN UJI STABILITAS TRANSFORMAN hardianto, dudi; gusnidar, tutus; singgih, marlia; musadad, amir; sumaryono, wahono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.551 KB) | DOI: 10.29122/jsti.v14i1.901

Abstract

Aspergillus terreus is a Saprophyte fungus that produces several secondary metabolites as lovastatin (anti-cholesterol drug) and itaconic acid (a polymer material). Lovastatin is one of the statin class of drugs that have efficacy as antihypercholesterolemic. Plasmid transformation is the introduction and incorporation of exogenous plasmid into cells orprotoplast. In this study, pTRLI plasmid (pTRI inserts containing lovE gene as a regulator gene in the biosynthesis of lovastatin) will be transformed by electroporation transformation. The purpose of this research is transformation of pTRLI plasmid into protoplasts of Aspergillus terreus by electroporation and obtain stable transformants. The research was initiated by isolation of pTRLI plasmid. Then pTRLI plasmid was determined purity and concentration by nanodrop. Furthermore, Protoplasts of Aspergillus terreus were isolated enzymatically by adding an enzyme which can degrade the cell wall of Aspergillus terreus which contains chitin and cellulose. PTRLI plasmid were transformed into protoplasts of Aspergillus terreus by electroporation. These transformants were grown in Czapek-Dox medium containing pyrithiamine agar and the number of transformants mg-1 of pTRLI plasmid was calculated. Transformants were selected to grow in Czapek-Dox medium containing piritiamin 1 mg l-1. The number of transformants produced 187 transformants mg-1 of PTRLI plasmid. Transformants are stable up to five generations by growing the transformants in Czapek-Dox medium agar containing piritiamin 1 mg l-1. The success of the transformation indicated by ptrA gene in transformants that can be amplified by PCR. The size of fragment DNA is 801 bp.
Co-Authors AE. Sukmawati, AE. Ahsogan, Kanaka L. Amir Musadad Anna Yuliana, Anna Dudi Hardianto, Dudi gusnidar, tutus Irma M. Puspitasari, Irma M. Julianti, Elin Sophi Damayanti Sri Priatni T. Gusdinar, T. Tri Suciati Wahono Sumaryono