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Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients Singgih, Marlia; Ahsogan, Kanaka L.; Puspitasari, Irma M.; Damayanti, Sophi
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known asa diet food that is recommended for diabetic and coronary heart disease patients. A relatively shortershelf life of paneer is considered to be a major hurdle in its production. The present preliminary studywas conducted to determine antibacterial activity of Lactobacillus plantarum against Eschericia coliand Bacillus cereus, to develop it as biopreservative agent in paneer before using it for dietary food ofdiabetic and coronary heart disease patients in next clinical study. The antibacterial activities were testedthrough Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)using microdilution well method. Biopreservative activity in paneer was tested using total plate countmethod based on time and temperature variations. Result showed that Lactobacillus plantarum inhibitedBacillus cereus and Eschericia coli with MIC of 3125 and 1562.5 Î¼g/mL whereas MBC was in a value of>6250 and >3125 Î¼g/mL, respectively. As a biopreservative agent, addition of Lactobacillus plantarumto paneer showed no bacterial growth until 7 days in room temperature and 9th day in cold temperature. Itis concluded that Lactobacillus plantarum could be used as a natural biopreservative agent for extendingthe shelf life of paneer. The paneer with addition of Lactobacillus plantarum as biopreservative will thenbe consumed by diabetic patient and coronary heart patients in next clinical study.

ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43, No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

ISOLATE OF HETEROTROPHIC MICROALGAE AS A POTENTIAL SOURCE FOR DOCOHEXAENOIC ACID (DHA) Julianti, Elin; Fathurohman, Mochamad; Damayanti, Sophi; Kartasasmita, Rahmana Emran
Marine Research in Indonesia Vol 43 No 2 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Docosahexaenoic acid (DHA) is one of essential fatty acids that are beneficial to health. Nowadays, the source of docosahexaenoic acid (DHA) is mainly obtained from fish which are extracted into fish oil products. However, the fish oil products still have some drawbacks in term of purity, acceptable flavor for costumers, and also their not environmental friendly production process. As an alternative solution, heterotrophic microalgae can be used as a potential source for DHA due to their excellence compared to fish oil products. The aim of this study is to isolate the heterotropic microalgae that can produce DHA. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) by using direct planting method. The morphology of pure microalgae colony were observed through light microscope and subsequently fermented for 14 days. Fatty acids were extracted and methylated through direct transesterification method. Identification and quantification of DHA were conducted by using gas chromatography. The results were four isolates of heterotropic microalgae, namely MTKC1, MTKC2, MTKC3, and MTKC4. The extract of MTKC2 that only showed the content of DHA with value of 9.2 % w/w. Therefore MTKC2 is a potential source for DHA. The MTKC2 was further identified by using molecular biology method and confirmed as Thraustochytrium aureum.

Reseptor P2Y G-Protein Couple Receptors (GPCRs): Target Menarik Pengembangan Obat Baru Damayanti, Sophi
JURNAL FARMASI GALENIKA Vol 2 No 01 (2015): JURNAL FARMASI GALENIKA
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.366 KB)

P2Y purinergik reseptor adalah bagian dari G-protein couple receptor (GPCRs), group reseptor yang merupakan target utama pada berbagai pengobatan. Hingga kini, delapan subtipe reseptor P2Y telahdiidentifikasi. Kelompok pertama dari P2Yadalah reseptor P2Y1, P2Y2, P2Y4, P2Y6, dan P2Y11. Reseptor P2Yditemukan di berbagai lokasi di dalam tubuh seperti otak, limfosit, paru-paru, sel darah dan lain-lain. Salah satu reseptor yaitu reseptor P2Y12 memainkan peran utama dalam aktivasi trombosit. Reseptor ini dilaporkan untuk mengambil bagian dalam amplifikasi dan penyelesaian aktivasi platelet dan agregasi antagonis reseptor P2Y12 yang telah dikenal untuk pengobatan adalah clopidogrel dan ticlopidin. Berdasarkan lokasi dan peran P2Y reseptor dalam berbagai fungsi tubuh, dapat disimpulkan bahwa P2Y reseptor merupakan salah satu reseptor yang dapat dijadikan pengembangan obat secara rasional berdasarkan target. 

StudiIn SilicoSenyawaTurunan8-Tersubtitusi-7-Methoxy-2h-Chromen-2-OneSebagaiPenghambatEnzim Telomerase Tahir, Masdiana; Damayanti, Sophi; Hadi, Daryono; Tjahyono, Tjahyono
Jurnal Farmasi Indonesia Vol 10, No 2 (2018)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v10i2.588

Adjustment of telomere length by telomerase is considered as a biological marker which determine the proliferation of cancer cells. The telomerase activity in cancer cells is potential to be targeted for cancer therapies. The aim of this study was QSAR studies to determine the descriptors that affect the anticancer activity of 8-substituted-7-methoxy-2H-chromen-2-one derivatives. The descriptors were calculated by using software MOE® 2009.10. to design new derivative compounds that have anticancer potency, to predict pharmacokinetic properties by using preADMET, and to predict tocixity by ADMET predictorTM and interaction predict to telomerase by using Autodock 4.2.6 The results showed QSAR equation was log IC50 = -1.358 (± 1.149) - 2.957 (± 1.019) × AM1_LUMO + 0.041 (± 0.011) × E_vdw + 2.367 (± 0.748) × glob + 0.487 (± 0.100) × log S. Fourteen of the new derivative compounds were designed based on QSAR equation revealed higher activity prediction than the lead compound and performed good absorption in the intestine. Compound (33) is predicted to have low permeability in Caco-2 cells and weakly bound to plasma proteins. Compound (50) predicted weakly bound to plasma proteins as well. Toxicity prediction showed that 9 derivatives compounds were predicted to have lower nearly equal toxicity to the toxicity of the lead compound. The study of the interaction of compounds with the receptor showed that derivatives (2), (4), (40) and (41) have the highest affinity to the receptor TERT with the binding free energy is −9.60 kcal / mol, −9.39 kcal / mol, −9.20 kcal / mol and -9.08 kcal / mol. Based on QSAR study, pharmacokinetic profile, toxicity, and the study of the interaction, four compounds have the potential to be develop as anticancer with telomerase inhibition.

StudiIn SilicoSenyawaTurunan8-Tersubtitusi-7-Methoxy-2h-Chromen-2-OneSebagaiPenghambatEnzim Telomerase Tahir, Masdiana; Damayanti, Sophi; Hadi, Daryono; Tjahyono, Tjahyono
Jurnal Farmasi Indonesia Vol 10, No 2 (2018)
Publisher : Jurnal Farmasi Indonesia

Adjustment of telomere length by telomerase is considered as a biological marker which determine the proliferation of cancer cells. The telomerase activity in cancer cells is potential to be targeted for cancer therapies. The aim of this study was QSAR studies to determine the descriptors that affect the anticancer activity of 8-substituted-7-methoxy-2H-chromen-2-one derivatives. The descriptors were calculated by using software MOEÂ® 2009.10. to design new derivative compounds that have anticancer potency, to predict pharmacokinetic properties by using preADMET, and to predict tocixity by ADMET predictorTM and interaction predict to telomerase by using Autodock 4.2.6Â The results showed QSAR equation was log IC50 = -1.358 (Â± 1.149) - 2.957 (Â± 1.019) Ã— AM1_LUMO + 0.041 (Â± 0.011) Ã— E_vdw + 2.367 (Â± 0.748) Ã— glob + 0.487 (Â± 0.100) Ã— log S. Fourteen of the new derivative compounds were designed based on QSAR equation revealed higher activity prediction than the lead compound and performed good absorption in the intestine. Compound (33) is predicted to have low permeability in Caco-2 cells and weakly bound to plasma proteins. Compound (50) predicted weakly bound to plasma proteins as well. Toxicity prediction showed that 9 derivatives compounds were predicted to have lower nearly equal toxicity to the toxicity of the lead compound. The study of the interaction of compounds with the receptor showed that derivatives (2), (4), (40) and (41) have the highest affinity to the receptor TERT with the binding free energy is âˆ’9.60 kcal / mol, âˆ’9.39 kcal / mol, âˆ’9.20 kcal / mol and -9.08 kcal / mol. Based on QSAR study, pharmacokinetic profile, toxicity, and the study of the interaction, four compounds have the potential to be develop as anticancer with telomerase inhibition.

Identification of Community Knowledge on Lead Contamination and Analysis of Lead Level in Fish Nurwulan, Sita; Damayanti, Sophi; Amalia, Lia
Pharmaceutical Sciences and Research
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Poisoning is an event of human exposure to poisonous substances that are harmful to the body at certain doses. Lead is a heavy metal that can cause toxicity, including neurotoxic effects, hypertension, digestive system disorders, bone and tooth growth disorders, immune system disorders, infertility, and fetal disorders. Fish is a source of nutrients that are often consumed by people, however can also accumulate lead. This study aimed to analyze the lead level in fish and identify the community knowledge level regarding lead contamination in fish. Identification of community knowledge levels regarding lead. Identification of community knowledge levels regarding lead contamination in fish was conducted on three hundred respondents consisting of School of Pharmacy (SF) Institut Teknologi Bandung (ITB) one hundred students, one hundred community, and one hundred housewives. This study also analyzed lead levels in six fish samples using Graphite Furnace Atomic Absorption Spectroscopy (GFAAS). As the result, SF ITB students had the highest level of knowledge in the definition of lead (97%), lead poisoning (98%), fish lead accumulation (75%), the poisoning effects of lead, and sources of lead. However, all of the respondents had a low level of knowledge regarding regulations about lead levels in fish. The lead levels analysis on fish showed that three out of six samples had lead levels that exceeded the limit of lead contamination (>0.2 g/kg). The study indicated that respondents’ knowledge regarding lead contamination in fish is still low and lead-contaminated fish is still being sold on the market.

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