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All Journal Indonesian Journal of Urology
Doddy M Soebadi
Departemen/SMF Urologi, FK Universitas Airlangga/RSU Dr. Soetomo, Surabaya
Health Professions Medicine & Pharmacology Public Health

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Journal : Indonesian Journal of Urology

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THE EFFECT OF N-ACETYL CYSTEINE TO SPERM MOTILITY, VIABILITY, AND CONCENTRATION OF SPRAGUE DAWLEY RATS WITH NICOTINE INHALATION EXPOSURE Waskito, Dwi; Renaldo, Johan; Soebadi, Doddy M
Indonesian Journal of Urology Vol 26 No 1 (2019)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v26i1.548

Abstract

Objective: To prove and analyze the difference of sperm motility, viability, and concentration of Sprague Dawley rats which were exposed to nicotine inhalation, with rats treated with N-acetyl cysteine (NAC) orally and exposed to nicotine inhalation. Material & Methods: Twenty seven rats were allocated into three group. Control group (C) (aquadest inhalation 1 mL/kgBW/day), treatment group 1 (N) (nicotine inhalation 1 mg/kgBW/day), and treatment group 2 (N-NAC) (nicotine inhalation 1 mg/kgBW/day and oral NAC administration 150 mg/kgBW/day), with all treatment were given for 30 days. Orchidectomy was performed on day 31 to collect semen sample for sperm analysis (motility, viability, and concentration). Results: There was a significant decrease in all parameters from N group compared to C group. Significant increase were found in sperm motility and viability parameters in the N-NAC treatment group compared to N group. While the sperm concentration parameters of the N-NAC group had a non-significant increase compared to N group. Conclusion: Exposure to nicotine inhalation decreased sperm motility, viability, and concentration of Sprague Dawley strain rats, and NAC had a protective effect on sperm of rats which was exposed to nicotine inhalation.
DIFFERENCES OF TESTOSTERONE LEVELS TO THE DURATION OF INHALED NICOTINE EXPOSURE IN SPRAGUE-DAWLEY MALE WHITE RATS Ramadhan, Ido Putra; Djojodimedjo, Tarmono; Soebadi, Doddy M
Indonesian Journal of Urology Vol 27 No 1 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i1.593

Abstract

Objective: To analyze the differences in testosterone level in young Sprague-Dawley white rats after exposure of inhaled nicotine and the recovery effect that occurs when nicotine exposure is stopped. Material & Methods: This is an experimental study, which variable measurements were taken after the experimental animals were treated. The experimental grouping process was carried out randomly, with repetitions of 12 experimental animals in each group and the control group as a comparison (negative control). This study using male Sprague-Dawley white rats as samples. Nicotine administration was carried out by inhalation using pure nicotine (C10H14N2), which was diluted using distilled water. Treatment group 1 (P1) was given nicotine exposure for 30 days, treatment group 2 (P2) for 15 days, and treatment group 3 (P3) for 15 days and then stopped for 15 days compared to the control group. Testosterone levels are measured using the ELISA (Enzyme-Linked Immunosorbent Assay) method in the morning. Results: The lowest testosterone levels were found in the P1 and significantly different from the P2 (p=0.029) and P3 (p=0.001). The results of the analysis show that a decrease in testosterone levels in mice is related to the duration of nicotine exposure. Conclusion: Testosterone levels in the control group were significantly higher compared to the treatment group that exposed by nicotine inhalation with a dose of 4 mg/kgBW/day for 15 days, and also there is a significant recovery effect on testosterone levels after the treatment was halted for 15 days.
EFFECTS OF X-RAY RADIATION EXPOSURE ON SPERM MOTILITY AND MORPHOLOGY CHANGES OF WISTAR STRAIN RATS Hariyoto, Bangun Oktavian; Soebadi, Doddy M; Soetojo, Soetojo; Soeprijanto, Bambang
Indonesian Journal of Urology Vol 27 No 1 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i1.599

Abstract

Objective: To analyze the differences of sperm motility and viability changes in Wistar Rats that exposed to X-ray radiation compared to Wistar Rats that were not exposed to X-ray radiation. Material and Methods: Experimental laboratory research within vivo design using Wistar strain rats as subjects. There are three treatment groups in this study: rats exposed to 50 mGy X-ray radiation, 100 mGy, and 200 mGy and one untreated control group. Radiation exposure was calibrated using the RTI Piranha dosimetry. After radiation exposure, at week 4, orchidectomy was performed in rats. Sperm analysis was carried out to determine sperm motility by direct observation and sperm viability by Hematoxylin Eosin (HE) staining observations using a light microscope. One Way ANOVA was used to compare motility and viability of rats spermatozoa in the treatment group compared to control group with a significant value of p<0.05. The analysis was continued with a Post Hoc test to determine the differences in each group. Results: The percentage of motile sperm decreased in all treatment groups compared to control group (p<0.05). There was no significant difference in mean sperm motility on rats exposed to 200 mGy X-ray radiation compared to 100 mGy X-ray radiation (p>0.05). Viability counts decreased in all treatment groups compared to control group (p<0.05) and the spermatozoa viability in 200 mGy radiation group was not significantly different from 100 mGy group (p>0.05). Conclusion: X-ray radiation exposure decreases sperm motility and viability in Wistar rats with the optimum doses 100 mGy.
COMPARISON OF BLADDER CANCER CELLS APOPTOSIS INDUCED BY METFORMIN, CISPLATIN, AND COMBINATION OF METFORMIN-CISPLATIN: IN VITRO STUDY Lumbangaol, Arifai; Hakim, Lukman; Soebadi, Doddy M; Djojodimedjo, Tarmono
Indonesian Journal of Urology Vol 27 No 1 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i1.600

Abstract

Objective: To evaluate the effect of metformin, cisplatin, and their combination on apoptosis of bladder cancer cells. Material & Methods: Urothelial cell lines 5637 were cultured until 80% confluence. Cells were exposed to metformin and cisplatin at certain doses for 24 and 48 hours. Cytotoxicity test was carried out by calculating the viability of bladder cancer cells using the MTT assay until IC50 of each drug was obtained. IC50 Metformin and Cisplatin obtained from the cytotoxicity test were used to induce apoptosis in bladder cancer cells using TUNEL assay. Additional combination doses of Metformin and cisplatin used to induce apoptosis were ¼ IC50 (metformin + cisplatin), ½ IC50 (metformin + cisplatin), and IC50 (metformin + cisplatin). Results: IC50 of metformin was 15 uM while cisplatin was 18 uM with a 48-hour exposure. There was a difference in the mean value of the apoptosis index in all treatment groups compared to control except for the group exposed to IC50 metformin and ¼ IC50 (metformin + cisplatin). Conclusion: Metformin administration solely is not able to increase bladder cancer cell apoptosis. Conversely, the administration of Cisplatin can significantly increase bladder cancer cell apoptosis. The combination of Metformin and Cisplatin can significantly increase bladder cancer cell apoptosis. The rate of apoptosis in line with an increase dose of the combination of these two drugs.
COMPARISON OF INTERLEUKIN-6, PROCALCITONIN AND C-REACTIVE PROTEIN AS A DIAGNOSTIC BIOMARKER IN PATIENTS UROSEPSIS Nursanto, Trisno Fajar; Soebadi, M. Ayodhia; Soebadi, Doddy M
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.516

Abstract

Objective: To compare the diagnostic value of Interleukin-6 (IL-6), Procalcitonin (PCT) and C-Reactive Protein (CRP) in detecting urosepsis. Material & Methods: This study is an analytical descriptive cross-sectional study about the diagnostic value of IL-6, PCT, and CRP to determine the diagnosis of urosepsis. The study sample consisted of all SIRS patients with urological disorders. The entire sample was examined by IL-6, PCT, CRP, and urine culture as the gold standard diagnosis. Data were analyzed using 2x2 table analysis and ROC curve to obtain AUC value. Results: There are a total of 36 samples in this study. With a cut-off 140 pg/ml, IL-6 provides a sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Positive Likelihood Ratio (+LR) and Negative Likelihood Ratio (-LR) was 95.8%, 83.3%, 92%, 90%, 5.75, and 0.05 with AUC 90.8% (95% IK 80.2% -100%, p<0.001). Sensitivity and specificity for PCT with cut off 0.65 ng/ml were 75% and 83.3%. The sensitivity and specificity for CRP with cut off 41 mg/l was 70.8% and 58.3%. The AUC of PCT and CRP was 81.1% and 66.5%. Conclusion: IL-6 has the best diagnostic value followed by PCT and CRP in urosepsis cases. IL-6 can be used as a biomarker diagnostic of urosepsis.
COMPARISON OF INTERLEUKIN-6, PROCALCITONIN AND C-REACTIVE PROTEIN AS A DIAGNOSTIC BIOMARKER IN PATIENTS UROSEPSIS Nursanto, Trisno Fajar; Soebadi, M. Ayodhia; Soebadi, Doddy M
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.516

Abstract

Objective: To compare the diagnostic value of Interleukin-6 (IL-6), Procalcitonin (PCT) and C-Reactive Protein (CRP) in detecting urosepsis. Material & Methods: This study is an analytical descriptive cross-sectional study about the diagnostic value of IL-6, PCT, and CRP to determine the diagnosis of urosepsis. The study sample consisted of all SIRS patients with urological disorders. The entire sample was examined by IL-6, PCT, CRP, and urine culture as the gold standard diagnosis. Data were analyzed using 2x2 table analysis and ROC curve to obtain AUC value. Results: There are a total of 36 samples in this study. With a cut-off 140 pg/ml, IL-6 provides a sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Positive Likelihood Ratio (+LR) and Negative Likelihood Ratio (-LR) was 95.8%, 83.3%, 92%, 90%, 5.75, and 0.05 with AUC 90.8% (95% IK 80.2% -100%, p<0.001). Sensitivity and specificity for PCT with cut off 0.65 ng/ml were 75% and 83.3%. The sensitivity and specificity for CRP with cut off 41 mg/l was 70.8% and 58.3%. The AUC of PCT and CRP was 81.1% and 66.5%. Conclusion: IL-6 has the best diagnostic value followed by PCT and CRP in urosepsis cases. IL-6 can be used as a biomarker diagnostic of urosepsis.
Co-Authors A.A. Ketut Agung Cahyawan W Aditya, Dimas Visa Agung Dwi Wahyu Widodo Ahmad Fajrial Fajrial Ariyo Sakso Bintoro, Ariyo Sakso Bambang Soeprijanto Boyke Soebhali, Boyke Brahmana Askandar Tjokroprawiro Dajtisoesanto, Wahjoe Djoyo M Boetoro, Djoyo M Endang Joewarini Fadhli Hasan Fathurrahman, Hasroni Fauriski Febrian Prapiska, Fauriski Febrian Halfian, Randa Hariyoto, Bangun Oktavian Hendromartono Hendromartono, Hendromartono heri budiono Holyness Nurdin Singadimedja Johan Renaldo Kartuti Debora, Kartuti Krismantoro, Rahmad Kurnia Penta Seputra Kurniawan, Andrie Rhomdhon Limantara, Ancelia Lukman Hakim Lumbangaol, Arifai Marzuki Yusuf, Marzuki Mohamad Ayodhia Soebadi Muhammad Yamin S Negara, Muhammad Surya Nindra Prasadja Nugroho, Achmad Nugroho, Ananta Cahyo Nur Budiyono Budiyono, Nur Budiyono Nursanto, Trisno Fajar Ogi Bahaurini Gumilar Pramanta, Aditya Pramesti, MP Budyandini D Rachmat Budi Prasetyo, Rachmat Budi Ramadhan, Ido Putra Rizaldi, Aril Rizaldi, Fikri Sabilal Alif septa surya wahyudi, septa surya Soebadi, M. Ayodhia Soetojo Soetojo Sunaryo Hardjowijoto Tarmono Djojodimedjo Telussa, Arley Sadra Triyono Karmawan Sukmana Pria wahjoe djatisoesanto Waskito, Dwi Widodo J P Widodo J Pudjirahardjo Widodo Pudjirahardjo Yasa, Muhammad Asro Abdih Yuanda, Rameshdo Yudi Y Ambeng