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<![CDATA[ANTIBAKTERI SENYAWA FENOLIK DARI DAUN SAPUTANGAN (Maniltoa Grandiflora (A. Gray) Scheff) ]]>
<![CDATA[JHON PATAR SINURAT]]>;
<![CDATA[SAADAH SIREGAR]]>
<![CDATA[ Jurnal Penelitian Farmasi & Herbal Vol 1 No 2 (2019): Jurnal Penelitian Farmasi & Herbal ]]>
Publisher : <![CDATA[Fakultas Farmasi Institut Kesehatan DELI HUSADA Deli Tua ]]>
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DOI: 10.36656/jpfh.v1i2.64
<![CDATA[The Phytochemical screening test to identify secondary metabolites of Saputangan leaves extract (Maniltoa grandiflora (A.Gray) Scheff) showed positive results on phenolic and terpenoid compounds. The reagent used for phenolic screening was 5% FeCl3 and reagent for screening terpenoid was 1% CeSO4 in 10% H2SO4. Antibacterial test was carried out by agar diffusion method on extracts of DMSO Saputangan leaves. Phenolic compound obtained from extract of Saputangan leaves were 36.96 gram where the result was obtained after maceration, partitioning and evaporation processes. Antibacterial of phenolic compound was observed based on inhibitory zone diameters of phenolic compounds formed using paper discs with a diameter of 6 mm. The diameters of the inhibition zone against Escherichia coli bacteria are 8.1; 8.5 and 9.7 mm larger than the diameter of the inhibition zone in Staphylococcus aureus bacteria at 7.3; 7.8 and 8.5 mm. Then it can be stated that DMSO extracts of phenolic compound with concentrations (0.25; 0.50 and 1.00 mg / ml) have antibacterial strength at the medium level where the average inhibition zone of S. aureus bacteria is 7.87 mm and the average inhibition zone in E. coli bacteria is 8.76 mm.]]>
<![CDATA[UJI SENYAWA FLAVONOID TOTAL DARI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA ANNUA) MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TAHUN 2019 ]]>
<![CDATA[Novandi Purba]]>;
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Romauli Anna Teresia Marbun]]>
<![CDATA[ Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM) ]]>
Publisher : <![CDATA[Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam ]]>
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DOI: 10.35451/jfm.v2i1.323
<![CDATA[ABSTRAK Herba binara (Artemisia Annua) is one of the plants that contains flavonoids which are efficacious as antioxidants and anticancer. The purpose of this study was to determine the total flavonoid compounds contained in ethanol extract of binara herbs (Artemisia Annua). Compounds in ethanol extract of binara herbs were identified by phytochemical screening. The results showed that the ethanol extract of binara herbs contained alkaloids, flavonoids, saponins, and tannins. Extraction was carried out in two stages, namely maceration stage with 96% ethanol and fat removal stage by hydrolysis using distilled water solvent. The extract was partitioned with ethyl acetate and n-hexane solvents, then the partitions were examined by TLC using the mobile phase of chloroform and ethyl acetate (7: 3). Ribbon patches that have the same Rf and color prices as the initial detection are taken and searched. Then analyzed using High Performance Liquid Chromatography (HPLC). Based on the wavelength as well as the TLC test, a partial structure which was strongly suspected of total flavonoids was 0.93 g. Kata Kunci :Herba Binara (Artemisia Annua), flavonoid, KLT, High Performance Liquid Chromatography(HPLC).]]>
<![CDATA[ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA) ]]>
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Suci Wulandari]]>;
<![CDATA[Rinaldo Berutu]]>
<![CDATA[ Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM) ]]>
Publisher : <![CDATA[Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam ]]>
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DOI: 10.35451/jfm.v3i2.684
<![CDATA[Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.]]>
<![CDATA[Penentuan Kadar Flavonoid Total Daun Saputangan (Maniltoa Grandiflora (A.Gray) Scheff) dan Kemampuannya sebagai Antioksidan ]]>
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Reh Malem Br Karo]]>
<![CDATA[ Jurnal Dunia Farmasi Vol 6, No 2 (2022): Edisi April ]]>
Publisher : <![CDATA[Program Studi Farmasi, Fakultas Farmasi dan Kesehatan, Institut Kesehatan Helvetia ]]>
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DOI: 10.33085/jdf.v6i2.5143
<![CDATA[Pendahuluan: Tumbuhan Saputangan biasanya hanya dimanfaatkan sebagai tanaman hias. Kenyataannya, daun saputangan mengandung senyawa flavonoid berdasarkan hasil skrining fitokimia yang dilakukan. Senyawa flavonoid memiliki aktivitas antioksidan yang dapat melindungi tubuh dari serangan radikal bebas dan dapat memperlambat munculnya penyakit kronis. Maka diperlukan untuk menentukan kadar senyawa flavonoid total di dalam daun saputangan dan mengukur aktivitas antioksidannya. Tujuan: Penentuan kadar flavonoid total menggunakan metode Spektrofotometri UV-Visibel dan mengukur IC50 sebagai antioksidan daun saputangan menggunakan metode DPPH (Diphenyl Picryl Hidrazil). Metode: Skrining menggunakan FeCl3, NH3, Alkali dan Pb(CH3COO)2. Proses maserasi dilakukan menggunakan etanol dan direndam dalam maserator selama lebih dari 24 jam. Pengukuran kadar flavonoid total menggunakan spektrofotometer UV-Visibel pada panjang gelombang kuersetin maksimum yaitu 442 nm. Hasil: Persamaan regresi linier untuk kurva kalibrasi quercetin yang dihasilkan adalah persamaan y = 0,0155x + 0,0387 dengan nilai korelasi (R2) sebesar 0,9943. Regresi linier antioksidan daun saputangan terhadap persentase hambatan adalah y = 0.6899x + 1.5616 dengan korelasi (R2) sebesar 0.9895. Daun Saputangan mengandung senyawa flavonoid yang terbukti berdasarkan hasil skrining. Sedangkan berat ekstrak setelah dipartisi menggunakan etil asetat dan n-heksana adalah 50 gr. Kadar flavonoid total pada daun saputangan adalah 33,87 mg QE/g ekstrak atau 33,87%. Nilai IC50 sebesar 70,2108 ppm yang dikategorikan sebagai antioksidan pada tingkat sedang. Kesimpulan: Kandungan flavonoid total adalah 3,87 mg QE/g ekstrak atau 33,87% dan dikategorikan sebagai antioksidan pada tingkat sedang.]]>
<![CDATA[Penentuan Kadar Vitamin C dalam Buah Pepaya dan Uji Antioksidan terhadap Vitamin C Buah Pepaya (Carica Pappaya L) ]]>
<![CDATA[Fahma Shufyani]]>;
<![CDATA[Jhon Patar Sinurat]]>
<![CDATA[ Jurnal Dunia Farmasi Vol 6, No 2 (2022): Edisi April ]]>
Publisher : <![CDATA[Program Studi Farmasi, Fakultas Farmasi dan Kesehatan, Institut Kesehatan Helvetia ]]>
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DOI: 10.33085/jdf.v6i2.5144
<![CDATA[Pendahuluan: Buah pepaya memiliki banyak keunggulan selain rasanya yang enak juga kaya akan vitamin C dan antioksidan. Kedua hal tersebut merupakan suatu kajian yang menarik bagi peneliti. Tujuan: Penelitian ini bertujuan untuk menganalisis kadar vitamin C dan aktivitas antioksidan dari buah tersebut. Metode: Kandungan vitamin C dapat dibuktikan berdasarkan hasil skrining menggunakan pereaksi Metilen Biru yang menghasilkan warna Biru – Memudar, pada uji NaOH + FeSO4 menghasilkan warna kuning dan pada penambahan reagen KMnO4 menghasilkan warna ungu memudar, sedangkan untuk penentuan kadar vitamin C dilakukan menggunakan spektrofotometri UV-Vis dan analisis aktivitas antioksidan dilakukan menggunakan metode DPPH. Pengukuran kadar vitamin C dimulai dengan penentuan panjang gelombang maksimum asam askorbat yaitu sebesar 263 nm. Larutan pembanding asam askorbat diukur absorbansinya pada lamda 263 nm yang menunjukkan peningkatan konsentrasi berbanding lurus dengan absorbansi yang dihasilkan. Hasil: Persamaan regresi linear untuk kurva kalibrasi asam askorbat yang dihasilkan yaitu persamaan y = 0.0165x + 0.0813 dengan nilai korelasi (R2) sebesar 0.9869. Hasil yang diperoleh pada penentuan kadar vitamin C ialah sebesar 113.33 mg/mL. Persamaan regresi linear yang dihasilkan berdasarkan data konsentrasi buah pepaya terhadap % inhibisi yaitu y = 0.8994x + 8.8905 dengan korelasi (R2) sebesar 0.9703. Sementara hasil pengukuran antioksidan dari buah pepaya dalam meredam DPPH menghasilkan nilai IC50 sebesar 45.7077 yang dikategorikan termasuk antioksidan level kuat. Kesimpulan: Kadar vitamin C sebesar 113.33 mg/mL dan nilai IC50 adalah 45.7077 merupakan antioksidan kuat.]]>
<![CDATA[Determination of Total Flavonoid Content of Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff) and Its Ability as Antioxidant ]]>
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Reh Malem Br Karo]]>;
<![CDATA[Rinaldo Berutu]]>
<![CDATA[ Jurnal Sains dan Kesehatan (J. Sains Kes.) Vol. 4 No. 3 (2022): Jurnal Sains dan Kesehatan (J. Sains Kes.) ]]>
Publisher : <![CDATA[Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia ]]>
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DOI: 10.25026/jsk.v4i3.1042
<![CDATA[Determination of phenolic content using uv-visible and measured IC50 as antioxidant of saputangan leaves. Methods: Screening was used FeCl3, NH3, Alkali and Pb(CH3COO)2. The maceration process was carried out using ethanol as a solvent and soaked for more than 24 hours. The measurement of total flavonoid levels began with determining the maximum wavelength of quercetin, which was 442 nm. The linear regression equation for the resulting quercetin calibration curve was the equation y = 0.0155x + 0.0387 with a correlation value (R2) of 0.9943. The linear regression of antioxidant of saputangan leaves on percentage of inhibition is y = 0.6899x + 1.5616 with a correlation (R2) of 0.9895. The Maniltoa grandiflora (A. Gray) Scheff leaves contained flavonoid compounds which are proven based on the results of screening. Meanwhile, the weight of the extract after partitioning using ethyl acetate and n-hexane was 50 gr. Total flavonoid levels in Maniltoa grandiflora (A. Gray) Scheff leaves were 33.87 mg QE/g extract or 33.87%. IC50 value of 70.2108 ppm which was categorized as an antioxidant at the medium (medium) level. Conclusions: Total flavonoid content was 3.87 mg QE/g extract or 33.87% and categorized as antioxidant on medium level.]]>
<![CDATA[Analisis kadar zat besi pada sari kedelai kemasan dengan metode spektrofotometri UV-VIS ]]>
<![CDATA[Reh Malem br Karo]]>;
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Fioni Fioni]]>;
<![CDATA[Dewi Fibrini]]>
<![CDATA[ Jurnal Prima Medika Sains Vol. 3 No. 2 (2021): Edisi Desember ]]>
Publisher : <![CDATA[Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia ]]>
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DOI: 10.34012/jpms.v3i2.2038
<![CDATA[Latar belakang: Kedelai adalah hasil pangan jenis kacang-kacangan yang sangat kaya akan nutrisi yang bermanfaat bagi kesehatan manusia. Tujuan: Untuk mengetahui keberadaan zat besi dan kadar zat besi dalam sari kedelai. Metode: Analisis kualitatif menggunakan pereaksi NaOH, K4[Fe(CN)6] dan K3[Fe(CN)6] dan analisis kuantitatif menggunakan spektrofotometer UV-Visibel pada panjang gelombang 248.3 nm. Hasil: Sari kedelai mengandung zat besi yang dibuktikan melalui analisis kualitatif menggunakan pereaksi NaOH, K4[Fe(CN)6] dan K3[Fe(CN)6] yang menghasilkan masing-masing endapan hijau, biru dan coklat yang artinya mengnadung zat besi. Persamaan regresi linear larutan standar besi adalah Y = 0.0154x + 0.1555 dengan korelasi (R2) sebesar 0.9981. Kadar zat besi dalam sari kedelai adalah 56.48 mg/ml dan termasuk dalam kelompok produk yang memiliki kadar zat besi yang cukup tinggi. Kesimpulan: Sari kedelai mengandung zat zat besi dengan kadar sebesar 56.48mg/ml.]]>
<![CDATA[Analysis of Total Terpenoids from Maniltoa Grandiflora (A. Gray) Scheff Leaves Using TLC and HPLC Methods ]]>
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Visensius Krisdianilo]]>;
<![CDATA[Reh Malem br Karo]]>;
<![CDATA[Rinaldo Berutu]]>
<![CDATA[ Stannum : Jurnal Sains dan Terapan Kimia Vol 2 No 2 (2020): Oktober 2020 ]]>
Publisher : <![CDATA[Department of Chemistry - Universitas Bangka Belitung ]]>
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DOI: 10.33019/jstk.v2i2.1976
<![CDATA[Terpenoids screening were carried out using Liebermann Burchard and Salkowski reagent on the extract of Saputangan leaves. It showed that the leaves contained terpenoid compounds with appeared of a reddish brown ring in the extract and a reddish brown stain appeared on the TLC plate tested with 1% CeSO4 reagent in 10% H2SO4. The macerate of saputangan leaves processed separation using the partition method (Liquid-liquid Extraction). Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. Furthermore, TLC analysis was performed on total terpenoids using n-hexane: ethyl acetate (80:20 v/v) solvent to obtain 11 separate stains on the TLC plate with different Rf each. Analysis was enhanced in HPLC using 100% acetonitrile and 0.1% phosphoric acid at a wavelength of 210 nm, a flow rate of 0.500 mL/min and eluted for 30 minutes. Based on the HPLC results, there were 25 peaks which indicated the presence of total terpenoid compounds with the highest peak being peak no. 8 (ret.time's 6.234, area's 8503532 and height's 276032), peak no. 9 (ret.time's 6.674, area's 3322572 and height's 141859) and peak no. 10 (ret.time's 7.288, area's 2758231 and height's 103927)]]>
<![CDATA[Antibacterial Activity of Flavonoid-Rich Fractions Of Citrus Maxima Peel Extract ]]>
<![CDATA[Reh Malem br Karo]]>;
<![CDATA[Putranto Manalu]]>;
<![CDATA[Jhon Patar Sinurat]]>
<![CDATA[ Stannum : Jurnal Sains dan Terapan Kimia Vol 2 No 2 (2020): Oktober 2020 ]]>
Publisher : <![CDATA[Department of Chemistry - Universitas Bangka Belitung ]]>
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DOI: 10.33019/jstk.v2i2.1977
<![CDATA[Natural products can be used as an alternative in the treatment of various diseases such as infectious diseases due to the bioactive compounds contained therein. Moreover nowdays, there are many antibiotic resistance in the treatment of infectious diseases. Citrus maxima is one of the natural products. Citrus maxima have been used for many diseases in traditional medicine.The aim of this study was to investigate the antibacterial activity of flavonoid-rich fractions of citrus maxima peel extract. The bioactive compounds contained in Citrus maxima peel were extracted by maceration method using 96% ethanol solvent. Fractionation was conducted using liquid-liquid extraction using a solvent of water and ethyl acetate obtained ethyl acetate fraction. In this fraction, the TLC test was carried out to confirm the presence of phenolic and flavonoid compounds. The antibacterial activity testing for ethyl acetate fraction against S.aureus and E.coli was determined by disk diffusion method with concentration of 25 ppm, 50 ppm, 75 ppm and 100 ppm. The ciprofloxacin and distilled water were used as positive and negative control, respectively. The result of this study showed that ethyl acetate fraction ( flavonoid-rich fractions) Of Citrus Maxima has potential as antibacterial for bacterial S.aureus and E.coli with medium inhibitory ability in all of concentration ranges. The highest inhibition zone for S.aureus was found at a concentration of 100 ppm while for E.coli was at a concentration of 75 ppm.]]>
<![CDATA[FORMULATION FOR MAKING HAND SANITIZER BASED ON DURIAN PEEL EXTRACT (DURIO ZIBETHINUS MURR) IN IMPROVING ANTIBACTERIAL ABILITY ]]>
<![CDATA[Margaretta Olivia Br Manik]]>;
<![CDATA[Jhon Patar Sinurat]]>;
<![CDATA[Reh Malem br Karo]]>;
<![CDATA[Rinaldo Berutu]]>
<![CDATA[ JURNAL KESMAS DAN GIZI (JKG) Vol. 5 No. 2 (2023): Jurnal Kesmas dan Gizi (JKG) ]]>
Publisher : <![CDATA[Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam ]]>
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DOI: 10.35451/jkg.v5i2.1665
<![CDATA[Maceration using ethanol solvent was chosen in extracting durian rind which was then evaporated to obtain 50 grams of durian peel extract. The results of the phytochemical screening test showed that the durian rind contains tannin compounds as evidenced by the addition of Iron (III) chloride reagent to produce a black solution. In addition, durian peel also contains terpenoid compounds which have been proven using the Lieberman Burchard reagent so that a blackish brown ring is produced in the extract. The best hand sanitizer product formulation is 75 mL of 96% ethanol, 2 mL of glycerin, 3 mL of 3% hydrogen peroxide, 10 mL of distilled water and 10 mL of a 6% concentration of durian peel extract solution. The hand sanitizer product has a liquid form, has a clear brownish color, has a distinctive durian rind aroma with a pH around 6 indicating that the hand sanitizer is suitable for use. Concentration of 6% Durian fruit peel hand sanitizer has better antibacterial ability than other concentrations with a large inhibition zone of 12.4 mm which is categorized as a strong level as an antibacterial substance.]]>
