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All Journal HAYATI Journal of Biosciences Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak dan Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI
Sri Sulandari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Veterinary

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Mitochondrial DNA variation of the Sumatran elephant in Sumatera Sri Sulandari
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.481 KB) | DOI: 10.11598/btb.2012.19.2.250

Abstract

A research on Mitochondrial DNA analysis of genetic diversity in Sumatran elephant (Elephas maximus sumatranus) was conducted in this study.  A 630 bp segment of mitochondrial DNA was amplified on 105 samples of Sumatran elephant from 5 locations in Sumatera (Bentayan, Sugihan, Bukit Salero Lahat, Seblat, Way Kambas) using a set of primers: MDL3 (5’-CCCACAAT-TAATGGGCCC-GGAGCG-3’) and MDL5 (5’-TTACATGAATTGGCAGCCA-ACCAG-3’). The objectives of this study is to generate mitochondrial DNA D-loop sequences for all the Sumatran elephant samples under this study and  to provide information haplotypes and nucleotide diversity of  Sumatran elephant populations.             A total of 105 PCR product were successfully sequenced perfectly, with an average length of about 616 base pairs. However, mitochondrial DNA fragments  for this analysis used the first 601 bases. Results showed six haplotypes (BP, BT, BS, BR, BX and BY) identified in Sumatera.  The most of the sampled individuals are the haplotipe BT. BX and BY are most likely new haplotypes..  All haplotype, except for the haplotipe BP  are belonging to the Sumatera clade. The haplotipe BX was derived from the haplotipe BT, and the haplotype  BY was derived from the haplotipe BS by one transversion respectively. The other substitutions in this network were the transitions. The haplotype BP is widely distribute from Sri Lanka, Sumatera, Peninsular Malay  and China). Although reported that the haplotype BU distributed Sumatera and Peninsular Malay, but BU haplotype not detected in this study.             Genetic distances within populations in Bentayan, Bukit Salero Lahat, Seblat, Sugihan and Way Kambas ranged from 0.0000 - 0003, and the genetic distance between the populations that is 0.0000 - 0. 0022. The distance between haplotypes of Sumatran elephant’s population is low.• The diversity of haplotypes and nucleotide in Sumatera island is low, the highest is  in the region of Buki Salero  Lahat and, lowest is in Bentayan and Sugihan. Overall, the results of analysis of Fu and Li's F * test statistic indicates that the population of Sumatran elephants in Sumatra is -0.78871, which means there is no inbreeding, but not significant at P> 0:10.   Keywords : Sumatran elephant, Elephas maximus  sumatranus, mitochondrial DNA, haplotype
COLLECTION OF MATERIAL DNA SAMPLES FROM BIRDS IN THE GUNUNG HALIMUN NATIONAL PARK (GHNP) FOR ESTABLISHING OF DNA BANK Sri Sulandari; Dwi Astuti; Agus Kundarmasno; Alwin Marakarmah; Satrio Wijamukti
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1080

Abstract

This study was conducted to collect material DNA samples from birds in the Gunung Halimun National Park (GHNP) for establishing of DNA bank.Ciptarasa, Geger Hanjuang and Cikaniki village areas were chosen as sites for sample collections in the GHNP.In order to take the sample from birds, transect lines were established at each site where series of mist nets were operated.The captured birds, both live- and died-birds, were identified. For the living birds, blood and/or shed feathers were taken from the bird prior to releasing.In case of died birds, tissue and liver were collected. The collected samples were transported to genetic laboratory at Zoological Division,Researh Center for Biology (RCB)-LIPI (The Indonesian Institute of Science) and kept in 4°C. In this collection, a total of 411 collected samples were obtained, coming from 25 families and 79 species.The 19-endemic birds were found in GHNP.
Co-Authors Agus Kundarmasno Alwin Marakarmah Cece Sumantri Dwi ASTUTI Harini Nurcahya Mariandayani JUMANTO HARJOSUDARMO M S A Zein M. Syamsul Arifin Zein Moch Syamsul Arifin Zein Moch Syamsul Arifin Zein RUSMILAH SUSENO Satrio Wijamukti Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Paryanti TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TIKE SARTIKA