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Resistensi Kacang Tanah Transgenik yang Membawa Gen cp PStV Stabil Sampai Tujuh Generasi Silang-Dalam terhadap Peanut Stripe Virus Hapsoro, Dwi; Aswidinnoor, Hajrial; Jumanto, Jumanto; Suseno, Rusmilah; Sudarsono, Sudarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Penyakit bilur kacang tanah yang disebabkan oleh peanut stripe virus (PStV)merupakan salah satu penyakit utama pada pertanaman kacang tanah (Arachishypogaea L.). Penggunaan varietas tahan merupakan alternatif paling efektif untukmengatasi penyakit tersebut. Rekayasa genetika merupakan metode efektif untukmendapatkan varietas kacang tanah yang resisten PStV. Tujuan penelitian ini adalahuntuk (1) mengetahui respons tanaman kacang tanah transgenik yang membawa gen cpPStV terhadap infeksi PStV dan (2) menguji stabilitas transgen sampai tujuh generasisilang-dalam. Tanaman kacang tanah transgenik cv. Gajah generasi T0, T1, T2, T3, T5,T6, dan T7 diinokulasi secara mekanik dengan PStV. Terdapat tiga jenis respons kacangtanah transgenik terhadap infeksi PStV, yaitu resisten, recovery, dan rentan. Padatanaman resisten gejala tidak muncul. Pada tanaman recovery, gejala chlorotic ringmottle muncul pada satu daun atau lebih, selanjutnya gejala tidak tampak pada daundaunyang tumbuh kemudian. Pada tanaman rentan, gejala severe blotch muncul padasuatu daun, selanjutnya gejala tersebut tetap muncul pada seluruh daun yang tumbuhkemudian. Transgen cp PStV tetap stabil setelah mengalami tujuh generasi silangdalam.Sejumlah galur murni kacang tanah transgenik yang resisten PStV telahdiidentifikasi.

Detection and Host Range Study of Virus Associated with Pepper Yellow Leaf Curl Disease SRI SULANDARI; RUSMILAH SUSENO; SRI HENDRASTUTI HIDAYAT; JUMANTO HARJOSUDARMO; SOEMARTONO SOSROMARSONO
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

High incidence of Pepper yellow leaf curl virus (PepYLCV) was observed in Indonesia since early 2000. Disease incidence in Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% on C. annuum. As an exception, the disease incidence on C. annuum cv. TM 999 was in the range of 70-100%. The causal agent of the disease, PepYLCV, was detected by polymerase chain reaction. Viral specific DNA fragment of the size ~1600 bp and ~550 bp was amplified from infected plants using two pairs of geminivirus universal primers pAL1v1978/pAL1c715, and pAv494/pAc1048, respectively. The PepYLCV has an intermediate host range including plants belonging to the family of Solanaceae, Leguminosae, and Compositae. The species belonging to the families of Cucurbitaceae, Malvaceae, Chenopodiaceae, and Amaranthaceae were resistant to the virus. Physalis floridana, is very prospective as a propagation host for the geminivirus infecting pepper. Nicotiana spp., cucumber, watermelon, cotton, and Sida sp. could be used as a differential host. Besides, Capsicum frutescens cv. Cakra, tomato, N. benthamiana, N. glutinosa, and Ageratum conyzoides could be used as indicator plants for the geminivirus infecting pepper. Key words: Pepper yellow leaf curl virus, disease incidence, host range

Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic NOOR AIDAWATI; SRI HENDRASTUTI HIDAYAT; PURNAMA HIDAYAT; RUSMILAH SUSENO; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus Ã¢â‚¬â€œIndonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato

Meyasa Klon Anggrek Laeliocattleya laurie Linn Westernberger Bebas Virus Melalui Kultur Meristem Apikal Diah Ratnadewi Lukman; Rusmilah Suseno; Edhi Sandra
Jurnal Ilmu Pertanian Indonesia Vol. 1 No. 2 (1991): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Apical meristems..

Geminivirus infecting pepper: symptom variation and transmission study Infection of geminiviruses has been reported to cause signz3cantyield loss on various crops. Polymerase chain reaction was able to detect geminivirus infection on pepper samples around Bogor and Cipanas, West Java. Symptom variation and transmission of the geminivirus infectingpepper was studied using five solanaceae plants with three dzgerent transmission mannec i.e. mechanical inoculation, side grafirng, and insect vector. Eliza S. Rusli; Sri H. Hidayat; Rusmilah Suseno; Budi Tjahjono
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 1 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Geminivirus infecting pepper: symptom variation and transmission study Infection of geminiviruses has been reported to cause signz3cantyield loss on various crops. Polymerase chain reaction was able to detect geminivirus infection on pepper samples around Bogor and Cipanas, West Java. Symptom variation and transmission of the geminivirus infectingpepper was studied using five solanaceae plants with three dzgerent transmission mannec i.e. mechanical inoculation, side grafirng, and insect vector. The transmission study showed that mechanical inoculation was not able to transmit the eminivirus, while side grafting and insect vector, Bemisia tabaci, caused infection with symptoms varies from yellow mosaic, leaf curl leaf distortion, to stunting of the plant. Higher infection was observed on pepper var. Hot Chilli through B. tabaci (8009) and side grafting (71.4%) than those on chilli pepper and tomato. DNA fiagment of - 1.7 kb was amplified wing PCRfim those plants showing symptoms, but no DNA fiagment was observedfrom symptomless eggplant and tobacco var. White Burley. Thus it can be concluded that the last two kina3 ofplants were resistant to the geminivirus infectingpepper.

It has been known that phytoplasma inducing witches broom disease can infect various kind of leguminous plants in Indonesia, among others soybean. Information on the resistance of various kind of soybean varieties to the phytoplasma is still limited. Experiments were conducted to eveluate the resistance of ten soybean varieties/lines to the phytoplasma. The evaluation was carried out by inoculation method through Orosius argentatus Evans, with two days of acquisition feeding period, ten days lat Asniwita Asniwita; Rusmilah Suseno; Sri Hendrastuti Hidayat; Budi Tjahjono
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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It has been known that phytoplasma inducing witches broom disease can infect various kind of leguminous plants in Indonesia, among others soybean. Information on the resistance of various kind of soybean varieties to the phytoplasma is still limited. Experiments were conducted to eveluate the resistance of ten soybean varieties/lines to the phytoplasma. The evaluation was carried out by inoculation method through Orosius argentatus Evans, with two days of acquisition feeding period, ten days latent period and two days inoculation feeding period. Observation was conducted on the presence of phytoplasma, incubation period, symptom and number & weight of seed. The result indicated that line Malang 3474 was resistant, Sindiro and Sriono were tolerant, while the other tested varieties/lines, i.e. Galunggung, Orba, Ringgit, Wilis, Malang 2999, Malang 2805 and Sicinang were susceptible

Biological and molecular variability among PStV (peanut stripe virus) strains isolated from various peanut growing areas in Indonesia was studied based on their pathogenicity and coat protein genes. Six strains of PStV causing different types of symptoms on peanut leaves were used to study biological and molecular variability among these strains. Biological variability of the PStV strains was evaluated based on their pathogenicity on peanut cultivars, Gajah and Landak. While analysis of the coat Hasriadi Mat Akin; Sudarsono Sudarsono; Rusmilah Suseno
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Biological and molecular variability among PStV (peanut stripe virus) strains isolated from various peanut growing areas in Indonesia was studied based on their pathogenicity and coat protein genes. Six strains of PStV causing different types of symptoms on peanut leaves were used to study biological and molecular variability among these strains. Biological variability of the PStV strains was evaluated based on their pathogenicity on peanut cultivars, Gajah and Landak. While analysis of the coat protein gene was used to study the molecular variability of PStV strains. Based on their pathogenicity, six PStV strains could be grouped into three groups, medium, strong, and very strong strains. Analysis of a coat protein gene showed the variability of the PStV strains were 0-2.1percent based on nucleotide sequence and 0-1.4percent based on predicted amino acid sequence. There was no correlation between biological and molecular variability.

Resistance of peanut varieties to phytoplasm. Recently, witches' broom disease on peanut is becoming more important in Indonesia The use of resistant varieties is very potential to overcome the yield loss caused by the disease. Ten peanut varieties were evaluated for their response to phytoplasma using insect vector transmission. Three categories of plant response were observed during the experiment, i.e. moderate resistant (var, Macan, Zebra, and Simpai), susceptible (var. Biawak, Treggili Sandi Nugroho; Rusmilah Suseno; Sri Hendrastuti Hidayati; Purnama Hidayat
Buletin Hama dan Penyakit Tumbuhan Vol. 12 No. 2 (2000): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Resistance of peanut varieties to phytoplasm. Recently, witches' broom disease on peanut is becoming more important in Indonesia The use of resistant varieties is very potential to overcome the yield loss caused by the disease. Ten peanut varieties were evaluated for their response to phytoplasma using insect vector transmission. Three categories of plant response were observed during the experiment, i.e. moderate resistant (var, Macan, Zebra, and Simpai), susceptible (var. Biawak, Treggiling, Pelanduk, and Kidang), very susceptible (var. Gajah, Tupai, and Banteng). The average seed weight decrease per plant due to the phytoplasma infection was 40.99 - 100%.

Resistensi Kacang Tanah Transgenik yang Membawa Gen cp PStV Stabil Sampai Tujuh Generasi Silang-Dalam terhadap Peanut Stripe Virus Dwi Hapsoro; Hajrial Aswidinnoor; Jumanto Jumanto; Rusmilah Suseno; Sudarsono Sudarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2662

Penyakit bilur kacang tanah yang disebabkan oleh peanut stripe virus (PStV)merupakan salah satu penyakit utama pada pertanaman kacang tanah (Arachishypogaea L.). Penggunaan varietas tahan merupakan alternatif paling efektif untukmengatasi penyakit tersebut. Rekayasa genetika merupakan metode efektif untukmendapatkan varietas kacang tanah yang resisten PStV. Tujuan penelitian ini adalahuntuk (1) mengetahui respons tanaman kacang tanah transgenik yang membawa gen cpPStV terhadap infeksi PStV dan (2) menguji stabilitas transgen sampai tujuh generasisilang-dalam. Tanaman kacang tanah transgenik cv. Gajah generasi T0, T1, T2, T3, T5,T6, dan T7 diinokulasi secara mekanik dengan PStV. Terdapat tiga jenis respons kacangtanah transgenik terhadap infeksi PStV, yaitu resisten, recovery, dan rentan. Padatanaman resisten gejala tidak muncul. Pada tanaman recovery, gejala chlorotic ringmottle muncul pada satu daun atau lebih, selanjutnya gejala tidak tampak pada daundaunyang tumbuh kemudian. Pada tanaman rentan, gejala severe blotch muncul padasuatu daun, selanjutnya gejala tersebut tetap muncul pada seluruh daun yang tumbuhkemudian. Transgen cp PStV tetap stabil setelah mengalami tujuh generasi silangdalam.Sejumlah galur murni kacang tanah transgenik yang resisten PStV telahdiidentifikasi.

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