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APPLICATION OF RAW SECONDARY METABOLITES FROM FOUR ENTOMOPATHOGENIC FUNGI AGAINST CHILLI DISEASE CAUSED BY VIRUSES Soesanto, Loekas; Prastyani, Noviana; Sri Utami, Darini; Manan, Abdul
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.220100-107

Abstract

Application of raw secondary metabolites from four entomopathogenic fungi against chilli disease caused by viruses. The purpose of this research was to investigate several kinds of raw secondary metabolits to decrease viral disease in chilli and inspect their side effect to plant growth. This research was conducted at experimental farm, Faculty of Agriculture, Jenderal Soedirman University from November 2018 to March 2019. The chilli seeds used for indicator plant were obtained from virus-symptomatic chilli. The raw secondary metabolites was collected from four microbial isolates used in this study, i.e. Metarhizium anisopliae, Beauveria bassiana (Papua isolate), Lecanicillium lecanii and B. bassiana Bio B10 (Jember isolate). The experiment was arranged in completely randomized design with five replications. Observation was performed on incubation period, disease intensity, AUDPC, germination percentage, plant height, number of leaves, and number of shoots. The result showed that raw secondary metabolites obtained from M. anisopliae gave the best capability to suppress disease development. Application of M. anisopliae raw secondary metabolites reduced incubation period, viral disease intensity as well as AUDPC in 34.22; 77.98 and 79.49%, respectively. The raw secondary metabolites of L. lecanii could increase percentage of germination, plant height, number of leaves, and number of shoots as 100; 38.96; 38.96 and 52.38%, respectively, compared to control.
KAJIAN BEBERAPA MEDIUM PENYERAP KMNO4 DAN SUHU TERHADAP PENYAKIT ANTRAKNOSA PADA PISANG KULTIVAR SUSU LEPAS PANEN KEMASAN PLASTIK POLIETILEN RONY AL AFGANI; LOEKAS SOESANTO; DARINI SRI UTAMI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Study of Several KMnO4 Absorbant Media and Temperature on Antracnose of Postharvest Susu Cultivar Banana Packaged in Polyethylene Plastic. A research was aimed to know the best KMnO4 absorbant media, temperature, and their combination on antracnose of postharvest susu cultivar banana packaged in polyethylene plastic was carried out at the Laboratory of Plant Disease, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. The banana with age of 3.5 months after flowering was taken from Sokawera Village, Somagede District, Banyumas Regency, Central Java. Split-Plot Design was used with the plastic. The main plot was room (25-28oC) and low (15-17oC) temperatures. The sub plot was without media and with or without inoculation, media of charcoal, coal, brick, cotton, and rockwool. Variable observed was incubation period, attact area, attact intensity, waste index, softy rate, and sugar content. Result of the research showed that the best media for absorbing KMnO4 was cotton. Low temperature could decrease softy rate of 64.75% and attact area of 92.78% compared to room one. Combination between cotton or rockwool and without inoculation at low temperature was the best treatment because of decreasing attact area ofColletotrichum musae and waste index for 100%. Inoculation of the fungus was not affect fungus attact in the KMnO4 and its combination with temperature. All treatments were not affect colour, aroma, and taste of the banana.
Pemberdayaan Kelompok Wanita Tani Melalui Optimalisasi Pemanfaatan Pekarangan dengan Budidaya Sayuran Organik Dataran Rendah Berbasis Kearifan Lokal dan Berkelanjutan Endang Warih Minarni; Darini Sri Utami; Nur Prihatiningsih
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 1 NOMOR 2 SEPTEMBER 2017 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.802 KB) | DOI: 10.30595/jppm.v1i2.1949

Abstract

The specific purpose of this activity is to increase the motivation of women in improving the quality of life and self-reliance in the family by optimizing the yard with organic vegetable farming in the lowlands based on Local Wisdom and Sustainable.  The strategy used was  the empowerment of target groups with the approach of Participatory Rural Appraisal, methods of education, training, demonstration plots, assistance and equipped with learning by doing techniques. The materials presented were: (1) garden  intensification technology with verticultur method, (2) organic vegetable cultivation technology based on liquid organic fertilizer, botanical pesticide and PGPR, (3) composting technology with enrichment of Trichoderma harzianum (Tricho-compos) activator of local microorganism, (4) technology of pest and disease control on environmentally friendly vegetable crops using botanical insecticides and microbial antagonists; (5) water use efficiency technology with drip irrigation; (6) good sorting, grading and packing technology, 7) empowerment technology to form groups and institutional strengthening and group management. The results of this activity are: (1) the target group / members The women farmer group has a very high curiosity about new information that can improve their standard of living,(2) technology transfer activities on the use of garden with organic vegetable cultivation received positive response from the members of the Group, (3) This devotional activity can encourage the pattern of mind and pattern of member action in organic vegetable cultivation by developing rural resources and sustainable local wisdom, (4) Verikultur cultivation technique with vertical paralon type on a land area of 10 m2 is economically profitable 
PEMANFAATAN BOKHASI MAHKOTA DEWA UNTUK PENGENDALIAN PENYAKIT LAYU FUSARIUM PADA CABAI MERAH Darini Sri Utami; Endang Mugiastuti
Agrin Vol 14, No 1 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.1.95

Abstract

Penelitian yang bertujuan untuk mengetahui pengaruh bokhasi dari mahkota dewa dalam pengendalianpenyakit layu Fusarium dan pengaruhnya terhadap hasil cabai merah. Penelitian menggunakan Rancangan AcakKelompok dengan 6 perlakuan, masing-masing diulang sebanyak 4 kali. Perlakuan yang dicoba adalah Fusariumoxysporum Schlecht konsentrasi 2 x 10 6 konidium per ml, tanpa mahkota dewa, 50 ml per tanaman. F.oxysporum dan air rebusan biji-cangkang mahkota dewa 40 g /100 ml air 50 ml/tanaman, F. oxysporum danbokhasi dari buah mahkota dewa yang berturut-turut ditambah laru, 200 g / tanaman, ditambah EM-4 sebanyak100 ml/tanaman, ditambah superdegra 200g /tanaman, F. oxysporum dan buah mahkota dewa, 2 buah/tanaman.Hasil penelitian menunjukkan bahwa perlakuan air rebusan biji-cangkang mahkota dewa dan bokhasi darimahkota dewa yang berturut-turut ditambah dengan laru, EM-4, superdegra dan perlakuan buah mahkota dewaberturut-turut tdk dapat menunda masa inkubasi, menekan populasi konidium dalam tanah, meningkatkan bobotbasah dan kering tanaman. Akan tetapi, air rebusan biji-cangkang mahkota dewa berpotensi menurunkanintensitas penyakit sebesar 22,03 %. Semua perlakuan tidak dapat meningkatkan hasil total cabai merah secaranyata, tetapi bokhasi dari mahkota dewa yang ditambah EM-4 atau air rebusan biji-cangkang mahkota dewaberpotensi meningkatkan hasil cabai merah masing-masing sebesar 73,18 % atau 64,32 %.Kata kunci: bokhasi mahkota dewa, pengendalian layu fusarium, cabai merah ABSTRACTThis research aimed at knowing the effect of the mahkota dewa bokhasi for controlling the disease andthe effect of the mahkota dewa bokhasi on the red chili yield. Randomized Block Design was used with sixtreatments and four replicates. The treatments tested were Fusarium oxysporum Schlecht without the mahkotadewa, F. oxysporum with the mahkota dewa seed and shell boiled water of 40 g/100 ml for 50 ml crop-1 ,F.oxysporum and the mahkota dewa fruits bokhasi added with decomposer agent (laru) of 200 g crop-1,F.oxysporum and the mahkota dewa fruits bokhasi added with EM-4 of 100 ml crop-1 , F. oxysporum and themahkota dewa fruits bokhasi added with superdegra of 200 g crop-1 , F. oxysporum and mahkota dewa fruits of 2fruits crop-1 .Result of the research showed that the treatments of the seed and shell boiled water or bokhasiadded with laru, EM-4, superdegra, and the fruis could not postpone incubation period, suppress the conidialpopulation in soil, and not increase the root weight. The mahkota dewa seed and shell boiled water treatment,however, had potency to decrease the disease intensity for 22,03%. All treatments could not increase the redchili yield, but the bokhasi made from the mahkota dewa fruits added with EM-4 and the mahkota dewa seed andshell boiled water had potency to increase the red chili yield for 74.18% and 64.32%, respectively.Key words: bokhasi mahkota dewa, fusarium wilt control, red chili.
KESESUAIAN TIGA BAKTERI ANTAGONIS DENGAN EMPAT JENIS MINYAK ATSIRI Resti Fajarfika; Heru Adi Djatmiko; Darini Sri Utami
Jurnal Ilmiah Media Agrosains Vol 5 No 1 (2019): Edisi Desember
Publisher : Unit Penelitian dan Pengabdian Masyarakat (UP2M) Politeknik Banjarnegara

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Abstract

Penelitian ini bertujuan untuk menguji kesesuaian tiga bakteri antagonis dengan empatjenis minyak atsiri. Pengendalian hayati menggunakan bakteri Bacillus sp. B298,Streptomyces spp. S4, dan Pseudomonas fluorescens dengan memadukan pengendaliannabati (minyak atsiri cengkeh, serai wangi, temulawak dan nilam) pada konsentrasi 0,04; 0,2;1; dan 5%, bakterisida (streptomycin sulfat 20%), air dan pelarut (isopropil alkohol, minyaktanah, dan tween 20) menggunakan metode cakram kertas. Hasil penelitian menunjukkanbahwa tiga bakteri antagonis tidak ada kesesuaian dengan konsentrasi empat jenis minyakatsiri dan bakterisida, tetapi ada kesesuaian terhadap air dan pelarut.