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All Journal Jurnal Fitopatologi Indonesia Pembangunan Pedesaan Prosiding AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Pengabdian Pada Masyarakat Jurnal SOLMA Agrin : Jurnal Penelitian Pertanian Biosaintifika: Journal of Biology & Biology Education JURNAL PENGABDIAN KEPADA MASYARAKAT Abditani : Jurnal Pengabdian Masyarakat Menara Perkebunan JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Agrotechnology Research Journal Jurnal Agro Wiralodra SINTA Journal (Science, Technology, and Agricultural) JURNAL AGROSAINS : Karya Kreatif dan Inovatif Media Pertanian
Endang Mugiastuti
Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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PEMANFAATAN Bacillus sp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU TOMAT AKIBAT SINERGI R. solanacaerum DAN Meloidogyne sp. Mugiastuti, Endang; Rahayuniati, Ruth Feti; Sulistyanto, Prasmaji
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Penelitian ini bertujuan untuk mengetahui kemampuan bakteri Bacillus sp. dan P. fluorescens dalam mengendalikan penyakit layu akibat sinergi M. incoqnita dan R.solanacearum pada tanaman tomat di rumah kaca. Pseudomonas fluorescens P8 merupakan bakteri antagonis yang terbaik untuk mengendalikan penyakit layu tomat dengan menekan masa inkubasi 95,39%, intensitas penyakit 69,95%, dan tingkat kerusakan akar karena nematode, serta untuk meningkatkan pertumbuhan tanaman dengan meningkatkan bobot segar tanaman 52,80 %, bobot akar tanaman 47,48%, dan jumlah buah 58,86%.
PENGENDALIAN PENYAKIT LAYU FUSARIUM TOMAT: APLIKASI ABU BAHAN ORGANIK DAN JAMUR ANTAGONIS CONTROL OF TOMATO FUSARIAL WILT: APPLICATION OF ORGANIC ASH AND ANTAGONISTIC FUNGI Rahayuniati, Ruth Feti; Mugiastuti, Endang
Pembangunan Pedesaan Vol 9, No 1 (2009)
Publisher : Pembangunan Pedesaan

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Abstract

The aims of this research were to determine the effect of organic ash and antagonistic fungi on controlling tomato Fusarium wilt. Factorial Completely Randomized Design has been used in hisresearch. There were paddy husk and legume ash as organic ash factor, and T. harzianum and Gvirensasantagonistic fungi factor. The result showed that combination of antagonistic fungi (Tharzianum and G. virens) and organic ash (paddy husk and legume ash) could suppress the disease growth, incubation period, disease severity, and disease rate. The effectiveness of the combination were about 36.79-43.30%.
KEEFEKTIFAN Bacillus sp. DAN Pseudomonas fluorescens MENGENDALIKAN Fusarium oxysporum f.sp. lycopersici DAN Meloidogyne sp. PENYEBAB PENYAKIT LAYU PADA TOMAT SECARA IN VITRO Rahayuniati, Ruth Feti; Mugiastuti, Endang
Pembangunan Pedesaan Vol 12, No 1 (2012)
Publisher : Pembangunan Pedesaan

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Abstract

Penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis Bacillus sp. dan Pseudomonas fluorescens dari perakaran tanaman tomat yang secara in vitro memiliki kemampuan kitinolitik, mampu menekan Fusarium oxysporum pada tanaman tomat secara invitro, dan memiliki kemampuan untuk menekan penetasan telur nematoda. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Pertanian Universitas Jenderal Soedirman. Hasil penelitian menunjukkan bahwa (1) Bacillus sp. dan Pseudomonas fluorescens yang berasal dari perakaran tanaman tomat bersifat kitinolitik, (2) kedua bakteri tersebut dapat menekan pertumbuhan F. oxysporum secara invitro sebesar 3,22-66,67%, dan (3) kedua bakteri tersebut secara in vitro dapat menghambat penetasa telur Meloidogyne sebesar 17,15-64,85%.
PEMANFAATAN ASAP CAIR UNTUK MENGENDALIKAN Fusarium oxysporum DAN Meloidogyne spp. THE USE OF LIQUID SMOKE TO CONTROL Fusarium oxysporum AND Meloidogyne spp. Mugiastuti, Endang; Manan, Abdul
Pembangunan Pedesaan Vol 9, No 1 (2009)
Publisher : Pembangunan Pedesaan

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Abstract

This research aimed at knowing 1) phenolic compound contents in the liquid, 2) kinds of tree dust as raw material of the liquid potentially, and 3) the liquid concentration effectively inhibiting growth of Fusarium oxysporum and Meloidogyne spp. in the laboratory. Both in vitro test of the liquid on F. oxysporum and the liquid test potentially on Meloidogyne spp. were designed by Randomized Block Design arranged with factorial. The first factor from the in vitro test was saw dust of albasia, waru, johar, or coconut trees and the second one was the liquid concentration, i.e., 0, 1, 2, or 3 percent. From the second test, the first factor was the same material and the second one was the concentration. Result of the research showed that 1) the liquid from waste of johar tree contained the highest phenol as 4,273.05 ppm, 2) the liquid from waste of albasia, waru, johar, and coconut trees potentially controlled the nematode in the laboratory with mortality up to 50%, while the most potential in inhibiting F. xysporum was the liquid from coconut tree, 3) concentration of was the best concentration in inhibiting F. oxysporum with the highest growth inhibition of colony diameter and dry weight was 90.23 and 90.04%, respectively, while concentration of 4% was the best concentration in resulting nematode mortality at the laboratory as 83.799%.
ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Soesanto, Loekas; Manan, Abdul; Wachjadi, Muljo; Mugiastuti, Endang
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma manan, Abdul manan; Nurtiati, Nurtiati; Mugiastuti, Endang
Jurnal SOLMA Vol 7 No 2 (2018)
Publisher : Uhamka Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/solma.v7i2.2160

Abstract

Kelompok tani “Rukun Tani I” Desa Sumbang ,  Kecamatan  Sumbang Kabupaten  Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang  merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma.  Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 %  dan 34,09 %. Aplikasi pestisida hayati  Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit  sebesar 34,16 %.
Application of Bio P60 and Bio T10 in Combination Against Phytophthora Wilt of Papaya Soesanto, Loekas; Kustam, Kustam; Mugiastuti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20389

Abstract

Papaya is one of the most widely cultivated horticultural plants. Phytophthora wilt is an important papaya disease which results in production losses. This research aimed to determine the effect of Bio P60 (raw secondary metabolites of Pseudomonas fluorescens P60) and Bio T10 (raw secondary metabolites of Trichoderma harzianum T10) application in combination on Phytophthora wilt and on growth of pepaya. The research was conducted on Californian pepaya farm, Linggasari Village, Kembaran District, Banyumas Regency. Randomized block design was used with six replicates and five treatments consisted of control (Mancozeb 80%), Bio T10 flush + Bio T10 spray, Bio T10 flush + Bio P60 spray, Bio P60 flush + Bio T10 spray, and Bio P60 flush + Bio P60 spray. Variables observed were percentage of healthy leaves, infection rate, number of healthy leaves, and number of healthy pepaya fruit. Result of the research showed that application of Bio P60 and Bio T10 effectively cured Phytophthora wilt with the percentage of healthy leaves as 69.19% compared to control. The combination was able to increase the number of healthy leaves the number of healthy papaya fruit. The novelty of this research is that raw secondary metabolites of biological agents proven to be able to overcome the papaya diseases, which so far cannot be solved. The implication for the development of science is one step ahead in overcoming plant diseases biologically by utilizing raw secondary metabolites. The benefits for the community can overcome papaya plant diseases organically, safely, and environmentally friendly, and inexpensive.
SOSIALISASI DAN APLIKASI METABOLIT SEKUNDER MIKROBA ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT DAN MENINGKATKAN HASIL TANAMAN JAGUNG DI KECAMATAN SUMBANG KABUPATEN BANYUMAS JAWA TENGAH Loekas Soesanto; Abdul Manan; Endang Mugiastuti
Jurnal Abditani Vol. 2 No. 2 (2019): Oktober
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.041 KB) | DOI: 10.31970/abditani.v2i0.32

Abstract

Kelompok tani “Mugi Rahayu” Desa Tambaksogra, Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman jagung. Permasalahan utama yang dihadapi dalam peningkatan produksinya adalah tingginya serangan penyakit tanaman. Sosialisasi pengelolaan penyakit jagung dengan menggunakan metabolit sekunder mikroba antagonis perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang penyakit tanaman jagung serta tindakan pengelolaannya dengan metabolit sekunder. Permasalahan mitra diatasi dengan beberapa inovasi teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis penyakit jagung, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan metabolit sekunder mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Pertumbuhan dan hasil jagung pada petak metabolit sekunder setara dibandingkan dengan petak petani dengan menggunakan pestisida kimia sintetik
Granular Formulation Test of Pseudomonas fluorescens P60 for Controling Bacterial Wilt (Ralstonia solanacearum) of Tomato In Planta Soesanto, Loekas; Mugiastuti, Endang; Khoeruriza, Khoeruriza
AGRIVITA, Journal of Agricultural Science Vol 41, No 3 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i3.2318

Abstract

Ralstonia solanacearum is the most devastating bacteria. Pseudomonas fluorescens P60 is a bacterial antagonist. This research aimed to study shelf life, antagonism and the effect of granular application of P. fluorescens P60 to control bacterial wilt and growth of tomato in planta. The research was conducted at the Plant Protection Laboratory and the screen house, Faculty of Agriculture, Jenderal Soedirman University, from October 2018 to March 2019. A randomized block design was used with six treatments and five replicates. The treatments were control, R. solanacearum + 1, 5, 10, and 15 g the granule, and bactericide (Agrimycine sulfate 20%). Variables observed were population density, clear zone, incubation period, disease incidence, disease intensity, area under disease progress curve (AUDPC), crop height, root length, crops fresh weights, and phenolic compound content qualitatively. Result showed that the formulation up to 10 weeks still performed a high P. fluorescens P60 population and good activity. All the granular and the bactericide effectively suppressed the disease indicated by the lenghtening incubation period of 22.77-26.25%, reducing the disease incidence as 60-85%, decreasing disease intensity as 65-85%, and decreasing AUDPC as 75.69-86.11%-days, increasing phenolic compound content qualitatively, and increasing crop height between 24.85-36.17%, and fresh weight between 46.04-57.13%.
APPLICATION OF RAW SECONDARY METABOLITES FROM TWO ISOLATES OF Trichoderma harzianum AGAINST ANTHRACNOSE ON RED CHILI PEPPER IN THE FIELD Soesanto, Loekas; Mugiastuti, Endang; Suyanto, Agus; Rahayuniati, Ruth Feti
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12019-27

Abstract

Anthracnose on red chili pepper is one of the highly damaging diseases that difficult to control bypesticides. This study aimed to determine the effect of raw secondary metabolites isolated from two isolates of Trichodermaharzianum towards anthracnose as well as the growth and yield of red chili pepper plants in the field. The research used arandomized block design with five treatments and seven replicates. The treatments tested were control, raw secondarymetabolites of T. harzianum T10 and T213, and their combination. The observed variables were incubation period, diseaseintensity, the late population density of T. harzianum, plant height, root length, fresh and dry weight of the plant and root,flowering time, number of fruits per plant, fresh weight of fruit, and phenolic compound content analysis. The result showedthat the raw secondary metabolites from the combination of the two T. harzianum isolates were the best treatments that couldlengthen the incubation period and decrease the disease intensity as 30.2 and 87.05%, respectively. However, these applicationscould not increase the growth components; while for the yield components, the application could improve the number of fruitsper plant and fresh weight of fruit as 15.33 and 34.53%, respectively.
Co-Authors Abdul Manan abdul manan Abdul Manan Abdul Manan Abdul Manan Abdul Manan Abdul manan manan Agus Dana Permana dan Rostaman ., Agus Dana Permana dan Rostaman Agus Suroto Agus Suyanto Alvin Adhyatputera Ramadhana Anang Khairul Rahman Anida Amalia Rahma DARINI SRI UTAMI Dyah Pancasiwi Eka Oktaviani Eka Oktaviani Elvina Hapsari Etik Wukir Tini Fenty Chakimatul Isnaeni Fida Suci Ersapoetri Fitrianti Fitrianti Hening Kurniasih Hidayatul Ilahiyyah Ilahiyyah, Hidayatul Khoeruriza, Khoeruriza Kurniasih Mucharomah Kustam, Kustam Loekas - SOESANTO Loekas Loekas Soesanto Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto manan, Abdul manan Muhammad Bahrudin Muljo Wachjadi Muljo Wachjadi Murti Wisnu Ragil SASTYAWAN Ni Wayan Anik Leana Nur Prihatiningsih Nurtiati Nurtiati Nurtiati, Nurtiati Pradiptha, Clarissa Nadya Prasmaji Sulistyanto Purwanto Purwanto Rostaman Rostaman Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Sari, Lintang Yunita Slamet Rohadi Soedarmono Soedarmono Susamto Somowiyarjo Tarjoko Tarjoko Y. M. Sugi Maryudani