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All Journal Jurnal Fitopatologi Indonesia Agrotrop : Journal on Agriculture Science AGRIVITA, Journal of Agricultural Science Jurnal Agro Agrin : Jurnal Penelitian Pertanian
Loekas Soesanto
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Soesanto, Loekas; Manan, Abdul; Wachjadi, Muljo; Mugiastuti, Endang
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat Endang Mugiastuti; Abdul Manan; Ruth Feti Rahayuniati; Loekas Soesanto
Jurnal Agro Vol 6, No 2 (2019)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/5397

Abstract

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan.  Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit  layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.
KAJIAN BEBERAPA MEDIUM PENYERAP KMNO4 DAN SUHU TERHADAP PENYAKIT ANTRAKNOSA PADA PISANG KULTIVAR SUSU LEPAS PANEN KEMASAN PLASTIK POLIETILEN RONY AL AFGANI; LOEKAS SOESANTO; DARINI SRI UTAMI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Study of Several KMnO4 Absorbant Media and Temperature on Antracnose of Postharvest Susu Cultivar Banana Packaged in Polyethylene Plastic. A research was aimed to know the best KMnO4 absorbant media, temperature, and their combination on antracnose of postharvest susu cultivar banana packaged in polyethylene plastic was carried out at the Laboratory of Plant Disease, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. The banana with age of 3.5 months after flowering was taken from Sokawera Village, Somagede District, Banyumas Regency, Central Java. Split-Plot Design was used with the plastic. The main plot was room (25-28oC) and low (15-17oC) temperatures. The sub plot was without media and with or without inoculation, media of charcoal, coal, brick, cotton, and rockwool. Variable observed was incubation period, attact area, attact intensity, waste index, softy rate, and sugar content. Result of the research showed that the best media for absorbing KMnO4 was cotton. Low temperature could decrease softy rate of 64.75% and attact area of 92.78% compared to room one. Combination between cotton or rockwool and without inoculation at low temperature was the best treatment because of decreasing attact area ofColletotrichum musae and waste index for 100%. Inoculation of the fungus was not affect fungus attact in the KMnO4 and its combination with temperature. All treatments were not affect colour, aroma, and taste of the banana.
ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Loekas Soesanto; Abdul Manan; Muljo Wachjadi; Endang Mugiastuti
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.227

Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Field Application of Trichoderma Suspension to Control Cacao Pod Rot (Phytophthora palmivora) Rina Sriwati; Tjut Chamzurn; Loekas Soesanto; Munazhirah Munazhirah
AGRIVITA, Journal of Agricultural Science Vol 41, No 1 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i1.2146

Abstract

Cacao pod rot caused by Phytophthora palmivora, is an important disease and contributes significant disease losses to global cocoa production. This research objective was to determine the effect of Trichoderma harzianum and T. virens suspensions to cacao pod rot disease on the field. This research was carried out in Pulo Hagu village, Pidie Regency, Aceh, Indonesia from March to July 2017. The single pattern randomized block design was adopted to evaluate three treatments, i.e. without suspension (control), suspensions of T. Harzianum, and T. virens for eight replications. Each replication consisted of three of experimental units. The result showed that both of Trichoderma species contained only Alkaloid metabolite based on Phytochemical test. On the field, the application of T. harzianum suspension reduced the percentage of fruit infection and disease intensity for 48.57 %, 46.04 % at 12 weeks after application (WAA) respectively. Based on the percentage reduction in the area of the spot between the metabolites T. harzianum suspension and control and T. virens and control are 47.24 % and 27.46 % at 87 WAA respectively. In addition, T. virens suppressed the percentage of infected fruit and the intensity of infected fruit for 40.61 % and 38.02 % at 12 WAA.
PENGUJIAN KEMAMPUAN MIKROBA ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN DAN LAYU BAKTERI PADA TANAMAN KENTANG DI DAERAH ENDEMIS Muljo Wachjadi; Loekas Soesanto; Abdul Manan; Endang Mugiastuti
Agrin Vol 17, No 2 (2013): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2013.17.2.202

Abstract

Penelitian bertujuan untuk mengetahui kemampuan beberapa mikroba antagonis untuk mengendalikanpenyakit hawar daun dan layu bakteri pada tanaman kentang di daerah endemik. Penelitian ini dilaksanakan dilahan kentang Desa Kejajar, Kecamatan Kejajar, Kabupaten Wonosobo, pada bulan Juni sampai Agustus 2013.Mikroba antagonis yang digunakan hasil isolasi dari pertanaman kentang dan telah diuji di rumah kaca danlapangan terbatas, yaitu dengan Bacillus sp. B2 dan B4, serta Pseudomonas sp. P19 dan P21. Berdasarkan hasilpenelitian yang telah dilaksanakan, dapat disimpulkan bahwa mikroba antagonis belum mampu mengendalikanpenyakit hawar daun dan layu bakteri, serta belum mampu meningkatkan pertumbuhan dan hasil tanamankentang. Akan tetapi, mikroba antagonis mampu mengimbas senyawa tanin pada tanaman kentang.Kata kunci: mikroba antagonis, penyakit hawar daun, layu bakteri, kentangABSTRACTThe research aimed at knowing ability of some antagonistic microbes to control leaf blight and bacterialwilt on potato at endemic field. This research was carried out at Kejajar Village, Kejajar Subdistrict, WonosoboRegency from June up to August 2013. The antagonists used were isolated from potato field and had been testedin the screen house and the limited field, i.e., Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Basedon the research result, the antagonists could not control leaf blight and bacterial wilt, and could not increasegrowth and yield of potato. However, the antagonists could induce tannin content of the crop.Key words: antagonistic microbes, leaf blight, bacterial wilt, potato.
PENGARUH PEMASTEURAN TANAH TUNGGAL ATAU DIGABUNG AGENSIA HAYATI TERHADAP PENYAKIT BUSUK HATI DI PEMBIBITAN PISANG Joko Haryono; Nur Prihatiningsih; Rahman A. Wardhana; Loekas Soesanto
Agrin Vol 12, No 2 (2008): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2008.12.2.91

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh pemasteuran medium digabung dengan agensia hayatidan agensia hayati yang paling efektif terhadap penyakit busuk hati. Penelitian dilakukan di PT NusantaraTropical fruit, Lampung Timur dengan rancangan Petak Terbagi dan diulang tiga kali. Petak utama adalahmedium dipasteur atau tidak. Anak-petak adalah control dengan air steril, Trichoderma harzianum isolat jahedan pisang, Pseudomonas fluorescens P60, dan Fusarium equiseti diisolasi dari akar pisang. Peubah yangdiamati adalah masa inkubasi, intensitas penyakit, populasi konidium Fusarium akhir, akar berpotensi terinfeksi,tinggi tanaman, jumlah daun, jumlah akar primer dan sekunder, panjang akar, dan berat akar. Hasil penelitianmenunjukkan bahwa perlakuan gabungan memberikan hasil positif khususnya menurunkan intensitas penyakitsampai 43,57%. Agensia hayati T. harzianum isolat pisang dan P. fluroescens P60 yang digabung denganpemasteuran medium dapat menekan intensitas penyakit masing-masing sebesar 63,08 dan 59,75%. Agensiahayati yang paling efektif adalah T. harzianum isolat pisang karena menekan kepadatan Fusarium, meningkatkantinggi tanaman, dan meningkatkan berat akar masing-masing sebesar 41,12, 39,00, dan 98,86%.Kata kunci: busuk hati, bibit pisang, pemasteuran, agensia hayati ABSTRACTThe objectives of this research were to know the effect of pasteurized media combined with biologicalagents and the most effective biological agent on heart rot disease. The research was carried out at PT NusantaraTropical Fruit, East Lampung designed by Split Plot Design and repeated three times. The main plot waspasteurized and unpasteurized media. The subplot was control with sterile water or fungicide, Trichodermaharzianum isolated from ginger or banana, Pseudomonas fluorescens P60, and Fusarium equiseti isolated frombanana root. Variables observed were incubation period, disease intensity, late Fusarium conidial population,potentially infected root, crop height, leave numbers, primary or secondary root numbers, root length, and rootweight. Result of the research indicated that the combination treatments gave positively result specially to reducedisease intensity of 43.57%. Biological agents of T. harzianum banana isolate and P. fluorescens P60 combinedwith the pasteurisation could suppress disease intensity of 63.08 and 59.57%, respectively. The most effectivebiocontrol agent was T. harzianum banana isolate because of suppressing Fusarium density, increasing plantheight, and increasing root weight as 41.12, 39.00, and 98.86%, respectively.Key words: heart rot, banana seedlings, pasteurization, biological agents 
Aplication of Trichoderma harzianum T10 Secondary Metabolites in Effervescent Tablets Formula towards Cucumber Damping-off Sinta Fajriatul Ismi; Loekas Soesanto; Endang Mugiastuti
Jurnal Fitopatologi Indonesia Vol 18 No 4 (2022)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.4.177-186

Abstract

Aplication of Trichoderma harzianum T10 Secondary Metabolites in Effervescent Tablets Formula towards Cucumber Damping-off Formulation of secondary metabolites of antagonistic fungi in effervescent tablets (water-soluble tablets, TLA) is a novelty. This research aimed to determine the effect of application of Trichoderma harzianum T10 secondary metabolites in effervescent tablets formulas on the in vitro growth of Pythium sp and on controlling damping-off in field pot testing. In vitro test consisted of four treatments, i.e. control and 1–3 tablets per 15 mL; while field pot testing consisted of five treatments, i.e. control and watering frequency from one up to four times with five replicates for each treatment. Variables observed were growth inhibition, incubation period, disease incidence, area under the disease progress curve (AUDPC), plant height, number of leaves, weight of fresh and dry plants, weight of fresh and dry roots, and phenolic compound. Data analysis indicated that T. harzianum T10 secondary metabolites in effervescent tablets formulas could inhibit the in vitro growth of Pythium sp.. One time application of T. harzianum T10 secondary metabolites in effervescent tablets formulas was able to control damping-off which is shown by delaying incubation period by 76.9%, reduce disease incidence by 85% and AUDPC by 85.35%-day; to increase plant growth by increasing plant height by 54.53%, number of leaves by 51.04%, weight of dry plants by 49.46%; and increase secondary metabolites (saponins, tannins, and hydroquinone) compound of plants.
Corynespora cassiicola Causes Black Spots on Papayas Kurniasih Mucharomah; Loekas Soesanto; Endang Mugiastuti
Jurnal Fitopatologi Indonesia Vol 16 No 5 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Abstract

Corynespora cassiicola Causes Black Spots on Papayas One of the problems in papaya production is black spot disease which causes the appearance of the fruit to be less attractive and lowers the market price. This study aimed to isolate, identify, and determine the level of pathogenicity of the cause of black spot disease on papaya var. California. Identification was carried out based on morphological characters, while in vivo experiment was conducted using a completely randomized design with five replications. The treatment involved the vertical position of the fruits (first, second, third, fourth, and fifth), all fruit were inoculated with pathogenic fungi. The results showed that disease symptoms on papaya fruit involving small yellowish to dark brown spots is caused by the fungus Corynespora cassiicola. The fungus C. cassiicola has black-brown colonies, the surface of the colonies is smooth with flat edges, and curved like velvet. This fungus has a few upright conidiophores bent, septa, single and some are branched, brown, while the conidia are single, slightly bent, and septa 2–12. The pathogenicity of this fungus is moderate. The fastest incubation period occurred in fruit with the fourth vertical position, the largest disease spot areas was in the fruit in the first vertical position, and the highest disease severity was in the fruit with the first vertical position.
Co-Authors Abdul Manan Ali Djamhuri DARINI SRI UTAMI Endang Mugiastuti Endang Mugiastuti Kurniasih Mucharomah Muljo Wachjadi Muljo Wachjadi Munazhirah Munazhirah Nur Prihatiningsih Rahman A. Wardhana Rina Sriwati RONY AL AFGANI Ruth Feti Rahayuniati Sinta Fajriatul Ismi Tjut Chamzurn