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All Journal Jurnal Sains dan Seni ITS Jurnal Teknologi Pangan Plumula : Berkala Ilmiah Agroteknologi Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL AGROHITA Agro Bali: Agricultural Journal Nusantara Science and Technology Proceedings Berkala Penelitian Hayati
Sutini
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Pembangunan Nasional
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Other

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Journal : Berkala Penelitian Hayati

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In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer Sutini Sutini; Widiwurjani Widiwurjani; Nora Augustien; Hadi Suhardjono; Guniarti Guniarti; Djoko Agus Purwanto; Wirdhatul Muslihatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.457 KB) | DOI: 10.23869/42

Abstract

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.
Growth and accumulation of flavan-3-ol in Camellia sinensis through callus culture and suspension culture method Sutini Sutini; Susilowati Susilowati; M. Rasjad Indra; Djoko Agus Purwanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.625 KB) | DOI: 10.23869/73

Abstract

This study was aimed to assess flavan-3-ol biomass in C. sinensis through callus cultures and suspension cultures derived from leaf explants. Callus initiation of both cultures were using Murashige and Skoog medium were enriched with plant growth regulators Naphthalene Acetic Acid 3.0 mg/L and kinetin 2.0 mg/L. The procedures in this study were: (1) callus initiation by cutting the leaves of C. sinensis shoots then planted on Murashige and Skoog medium that were enriched with plant growth regulators, (2) sub callus culture on fresh medium that enriched with the same growth regu-lators, (3) suspension culture initiation of liquid callus, (4) growth examination of callus and suspension cultures in week 12, (5) examination of qualitative-quantitative content of flavan-3-olin suspension cultures at week 4. The results show that suspension cultures contain biomass flavan-3-ol that increase in the same manner of the increase of callus age and weight.
MENINGKATKAN PRODUKSI FLAVAN-3-OL MELALUI KALUS Camellia sinensis L. DENGAN ELISATOR CU2+ Sutini B; Tatik W; Wahyu W; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/297

Abstract

Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The difficulty in obtaining Flavan-3-ol out of tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore flavan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It can effectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants on medium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callus growth, (4) Observation over qualitative characterizes of flavan 3-ol. The result of the research using Cu 2+ elicitor shows that the production of flavan-3-ol increases by 12.5%.
Co-Authors Agus Purwanto Alvhi Widya Calandry Alvriado Igo Fransiscus Siahaan Augustien, Nora Ayu Silviana Carolina Rosa Siallagan Didik Utomo Pribadi Djoko Agus Purwanto Felicitas Deru Dewanti Guniarti Guniarti Guniarti Guniarti Hadi Suhardjono Juli Santoso M. Rasjad Indra Milatur Royyana Muslihatin, Wirda Nora Agustien Nora Augustien Nora Augustien Pribadi, Didik Huswo Pribadi, Didik Utomo Ramadhani Mahendra Kusuma Shofiyah Zanirah Sukendah Sukendah Susilowati Susilowati Sutiman B. Sumitro Tatik W Wahyu W Widiwuriani Widiwurjani Zulfikar Alvin Naufal