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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
T. A. Lestari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Produksi Pigmen Karotenoid oleh Khamir Phaffia rhodozyma yang Diperlakukan dengan Radiasi Sinar UV Sri Pujiyanto; Wijanarka Wijanarka; Endang Kusdiyantini; T. A. Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.146

Abstract

Carotenoid pigment is an essential element in aquaculture, since it gives characteristic of color on shrimp and fish. Carotenoid pigments can be produced microbiologically using Paffia rhodozyma. Genetic improvement of the yeast, one of which can be accomplished by radiation mutation, will increase the production of carotenoid pigments. The aims of this study were to mutate P. rhodozyma using UV irradiation and to figure out pigment production by the mutant strains resulting from 30 minute-irradiation. Irradiated culture was incubated in dark condition and plated onto YMA media. Grown mutant colonies were collected in order to test for their pigment production. Pigment production was measured on the basis of extinction coefficient of 1%. The results showed that mutant strain encoded with MUV-1 produced the highest pigment at 179.96 mg/g dry weight cell, higher than the wild type (63.20 mg/g dry weight cell).
Co-Authors Endang Kusdiyantini Sri Pujiyanto Wijanarka Wijanarka