Tri Maryono
Bidang Proteksi Tanaman Jurusan Agroteknologi, Fakultas Pertanian, Universitas Lampung

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EFIKASI ISOLAT TRICHODERMA TERPILIH DENGAN BAHAN ORGANIK UNTUK MENGENDALIKAN PENYAKIT BUSUK PANGKAL BATANG PADA LADA DI LAPANGAN Ginting, Cipta; Prasetyo, Joko; Nurhidayat, Aris; Maryono, Tri
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.947 KB) | DOI: 10.23960/j.hptt.11777-83

Abstract

Efficacy of selected Trichoderma isolate and organic matter to control foot rot of black pepper in the field. The objective of this experiment was to determine the efficacy of selected Trichoderma isolatand organic matter to control the disease. Dual culture method was used to select a Trichoderma isolate. The experiment to evaluate the efficacy of selected isolate consisted of control (no application of T. harzianum or organic matter), T. harzianum and rice straw, and T. harzianum and coffee husk. As starter, T. harzianum was grown in broken rice. Two liters of organic matter was infested with suspension of 10 g starter in 100 ml steril water and incubated for 2 weeks. The mixture was applied around the base of black pepper stem. The results showed that all plants treated with T. harzianum and organic matter did not show disease symptom. Ten percent of the control plants showed symptoms. However, statistical analysis showed that the occurrence of the disease was not significantly different between treatments. Applications ofT. harzianum and rice straw increased the density of Trichoderma for 1 and 2 months after application. T. harzianum and coffee husk increased the density of the fungus 1 month after application.
PENGARUH PENYARUNGAN BUAH DAN APLIKASI ASAM FOSFIT TERHADAP HAMA PENGGEREK DAN PENYAKIT BUSUK BUAH KAKAO Bastian, Muhammad Deri; Prasetyo, Joko; Maryono, Tri; Susilo, F. X.
Jurnal Agrotek Tropika Vol 3, No 1 (2015)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.521 KB) | DOI: 10.23960/jat.v3i1.1973

Abstract

Hama penggerek buah dan penyakit busuk buah adalah masalah utama dalam budidaya kakao. Larva penggerek buah kakao setelah menetas langsung masuk dan berkembang di dalam buah. Sedangkan spora patogen mudah berkecambah bila kondisi lembab. Penyarungan buah kakao sejak masih muda dapat menjadi upaya pencegahan penggerek buah dan penyakit busuk buah yang baik. Namun penyarungan buah mengakibatkan kondisi buah menjadi lembab sehingga busuk buah kakao meningkat. Untuk menekan penyakit busuk buah kakao maka perlu dilakukan penyemprotan fungisida, sebelum buah disarungi. Salah satu bahan aktif fungisida yang diteliti efektif untuk menekan pertumbuhan Phytophthora adalah asam fosfit. Penelitian inibertujuan mengetahui pengaruh penyarungan buah dan aplikasi asam fosfit terhadap hama penggerek dan penyakit busuk buah kakao. Penelitian ini dilakukan dengan menggunakan rancangan faktorial menggunakan dua faktor, yaitu penyarungan buah dan penyemprotan fungisida asam fosfit. Data hasil pengamatan dianalisis secara statistik deskriptif. Hasil penelitian menunjukkan penyarungan buah dapat melindungi buah dari hama penggerek buah, namun penyarungan buah menyebabkan buah yang terserang busuk buah menjadi meningkat. Aplikasi fungisida berbahan aktif asam fosfit terlihat dapat mengendalikan busuk buah kakao jika buah tidak disarungi plastik, namun aplikasi fungisida berbahan aktif asam fosfit menjadi lebih rendah keefektifannya jika buah disarungi.
PENGARUH FRAKSI EKSTRAK DAUN PACAR CINA (AGLAIA ODORATA L.) TERHADAP PERTUMBUHAN COLLETOTRICHUM CAPSICI PENYEBAB PENYAKIT ANTRAKNOSA PADA CABAI (CAPSICUM ANNUUM L.) SECARA IN VITRO ., Efri; Aeny, Titik Nur; Maryono, Tri; Ronalddi, Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.079 KB) | DOI: 10.23960/j.hptt.217179-184

Abstract

Influence of Aglaia odorata L. leaf extract on in vitro growth of chilli antrachnose fungi (Colletotrichum capsici). Pacar cina (Aglaia odorata L.) is known as one of the potential herbs as fungicides. The objective of this study was to determine The effectiveness of various leaf extract fractions of pacar cina as a fungicide to suppress the in vitro growth of C. capsici, The causal agent of anthracnose disease on chili. This study consisted of two sub-experiments. The first experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with ethanol solvent with a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 0.2 % (synthetic fungicides) and control (without fungicide). The Second experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with the solven of n-hexane at a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 2% (synthetic fungicides), and control (without fungicides). The treatmentswerearranged by completely randomized design (CRD) with three replications. Variables measured in this experiment were colony diameter and density of spores of C. capsici. Data were analyzed by analysis of variance (ANOVA) and differences between the mean values was tested by Duncan Multiple Range Test (DMRT) with the level of 5% significant different. Additionally, leaf extract that effectively inhibited the growth of C. capsici was the fraction of the leak extract with 80% ethanol solvent and 10%, 60%, and 90% n-hexanesolvent. The result showed that the leaf extract fraction of pacar cina was potentially used as fungicide to inhibit the growth of C. capsici.
Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Tri Maryono; Ani Widiastuti; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 13 No 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.321 KB) | DOI: 10.14692/jfi.13.2.67–71

Abstract

Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26’ 16.6” S dan 104°40’ 09.8” E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.
Komponen Epidemi Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Tri Maryono; Ani Widiastuti; Rudi Hari Murti; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.49-60

Abstract

Epidemic Components of Sugarcane Root and Basal Stem Rot In South Sumatra Root and basal stem rot disease is one of new disease that currently was became a emerging problem on sugarcane plantations in Lampung and South Sumatra. The research was aimed to study the model of disease progression, AUDPC, infection rate (r), and the influence of weather and soil condition (physical and chemical properties of soil) on the development of sugarcane root and basal stem rot disease. The research was conducted on sugar cane plantation in South Sumatra. The disease incidence in three varieties was observed weekly in the field which had infected plant in previous year.. The data were analyzed to develop model of the disease progression, AUDPC, and the rate of root and basal stem rot disease. Weekly weather data was acquired from weather station of Sultan Mahmud Badaruddin Airport, Palembang. Soil sample was taken from three disease category i.e moderate (disease incidence 25,1% - 50%), severe (disease incidence 50,1% - 75%), and healthy (no disease). The results showed that the disease develops following monomolecular and logistic models. The AUPDC and infection rate (r) in the monomolecular model were lower than in the logistic model. The direct effect coefficient of rainfall and relative humidity (RH) on the disease were positive (1.27 and 0.46 respectively), contrary the temperature and duration of irradiation which had a negative coefficient (-0.33 and -0.45 respectively). Meanwhile, K availability and permeability give a positive effect on the disease, while the total N and Fe availability give a negative effect on the disease.
PENYAKIT AKAR GANODERMA PADA SENGON DI SLEMAN, YOGYAKARTA Tri Maryono
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 1 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.237 KB) | DOI: 10.20886/jpth.2020.14.1.55-61

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Ganoderma root disease in the community forest of sengon is one of the limiting factor to increase the productivity of sengon wood. This disease has been reported in West Java, East Java, South Sumatra and South Kalimantan. The survey was conducted to determine the existence of Ganoderma root disease in three sengon fields in Sleman, Yogyakarta. The existence of Ganoderma root disease was recognized based on the symptoms of diseased plants and the present of Ganoderma basidiocarp on the diseased sengon stem. The survey results on three sengon fields was succeed in finding diseased plants and also the basidiocarp of Ganoderma on the basal of the diseased plant stem. Symptoms that have been found was either dead or dying trees (few leaves left). The basidiocarp of Ganoderma on the diseased plants varies in characteristic while its young and mature. The basidiospores is ellipsoid, truncate, rough (coarsely echinulated), and double-walled. The Ganoderma colony was white and turned to yellow orange from the center. Based on this study the Ganoderma root disease in sengon plants has developed in Sleman, Yogyakarta. The presence of dead or dying trees with few leaves left between healthy sengon trees and the presence of Ganoderma basidiocarp in diseased trees are indicator of the presence of root disease in the sengon field caused by Ganoderma.
PENURUNAN KEPARAHAN PENYAKIT BUSUK PANGKAL BATANG PADA LADA AKIBAT APLIKASI BAHAN ORGANIK DAN TRICHODERMA HARZIANUM Cipta Ginting; Tri Maryono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.509 KB) | DOI: 10.23960/j.hptt.212162-168

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Foot rot of black pepper (Piper nigrum L.) caused by Phytophthora capsici Leionian is commonly considered as one of the most important problems in black pepper production. The objectives of this research was to determine the influence of organic matter and Trichoderma spp. on the severity of foot rot of black pepper. On the first experiment, five Trichoderma mycelial plugs were applied on planting medium consisting of soil, organic matter, and sand (2:2:1, v/v), then incubated for 7 days. Black pepper plants were transferred to the planting medium and after 14 days were inoculated on the stems. The variable observed was disease severity on the stem. On the second test, Trichoderma that was grown on broken rice (menir) as starter was applied into the planting medium which consists of soil and sand (2:1, v/v). Two weeks later, black pepper plants were inoculated on the leaves and stems. The variables observed were the diameter of necrotic on the leaves and disease severity on the stem. On the first experiment when 15 isolates of Trichoderma spp. were tested, five isolates (T. harzianum isolates 6, 8, 9, 10, and 15) significantly reduced disease severity. However, on the second test when four out of the five effective isolates were retested, none was found to significantly reduce disease severity on leaves or stems.
EFIKASI TRICHODERMA HARZIANUM DENGAN BERBAGAI BAHAN ORGANIK DALAM PENGENDALIAN PENYAKIT BUSUK PANGKAL BATANG PADA LADA Cipta Ginting; Tri Maryono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.407 KB) | DOI: 10.23960/j.hptt.211147-156

Abstract

The objective of this research was to determine the influence of the kinds of organic matter on the efficacy of Trichoderma harzianum Rifai to control foot rot of black pepper caused by Phytophthora capsici Leonian. Trichoderma spp. were isolated from suppressive and non-suppressive soils taken from black pepper fields with high disease incidence. Screening of Trichoderma spp. isolates was conducted through antagonistic test with dual culture technique. Treatments were arranged in a completely randomized design with six replications. Treatments were rice husk, rice straw, wood dust, Arachis pintoi, mixture of the four organic matters, the mixture without T. harzianum, and without organic matter. The test was conducted in greenhouse with media consisted of soil, organic matter, and sand (2 : 2 : 1, v/v). After being otoclaved, the medium was infested with T. harzianum and P. capsici each with five mycelium plugs of 1-cm diameter. Black pepper seedlings were planted 5 days after fungal infestation. After planting the seedlings, five leaf cuts were partly inserted into the soil on each pot. The variables observed were disease incidence on the leaf cuts inserted into the soil and disease severity on the stems and roots. The results show that all 16 Trichoderma isolates inhibited P. capsici colonies and some isolates showed stronger inhibition than the others. T. harzianum reduced disease severity, but there was no effect of the kinds of organic matter on the ability of T. harzianum to control foot rot.
INVENTARISASI JAMUR-JAMUR PATOGEN PADA BUAH JERUK (CITRUS sp.) DI BEBERAPA PASAR DI BANDAR LAMPUNG Deciana Deciana; Muhammad Nurdin; Tri Maryono; Suskandini Ratih D.
Jurnal Agrotek Tropika Vol 2, No 2 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.506 KB) | DOI: 10.23960/jat.v2i2.2083

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Salah satu masalah mendasar dari rendahnya mutu buah jeruk yang dihasilkan di dalam negeri adalah serangan patogen. Patogen yang banyak menyerang buah jeruk adalah jamur. Aktivitas jamur selama pertumbuhannya pada komoditi pangan dapat menyebabkan kerugian dan penurunan kualitas pangan sehingga produk pangan tersebut tidak layak untuk dikonsumsi dan diperdagangkan. Inventarisasi merupakan suatu langkah awal dalam studi penyakit guna menentukan langkah langkah selanjutnya. Penelitian ini berupa survei terhadap buah jeruk yang berasal dari beberapa pasar tradisional dan beberapa pasar modern di Bandar Lampung. Sampel buah jeruk yang diambil adalah jeruk lokal, yaitu jeruk medan. Setiap pasar diambil dua kali sampel buah, sehingga total pengambilan sebanyak empat kali. Dari hasil identifikasi, sampel buah jeruk yang didapat dari pasar tradisional maupun pasar modern ditemukan 5 jenis jamur, yaitu jamur Aspergillus sp., Colletrotrichum sp., Fusarium sp., Geotrichum sp., dan Penicillium sp. dengan rerata persentase pemunculan jamur di pasar tradisional masing-masing 32,27%, 26,38%, 28,80%, 10,07%, dan 2,5%, sedangkan di pasar modern yaitu jamur Colletrotrichum sp. 36,45% , Aspergillus sp. 23,40%, Fusarium sp. 14,40%, dan Geotrichum sp. 25,74%.
PENGARUH KONSENTRASI EKSTRAK DAN TINGKAT KEMATANGAN DAUN KERSEN TERHADAP PERTUMBUHAN Colletotrichum gloeosporioides DAN INTENSITAS PENYAKIT ANTRAKNOSA PADA BUAH PEPAYA Bella Mahesa; Efri Efri; Selvi Helina; Tri Maryono
Jurnal Agrotek Tropika Vol 10, No 1 (2022): JURNAL AGROTEK TROPIKA VOL 10, JANUARI 2022
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i1.5627

Abstract

Penyakit antraknosa merupakan penyakit utama dalam budidaya pepaya yang disebabkan oleh Colletotrichum gloeosporioides.  Daun kersen dapat menghambat pertumbuhan jamur C. gloeosporioides karena mengandung kelompok senyawa flavonoid, tanin, saponin, dan alkoloid.  Penelitian ini bertujuan untuk mengetahui konsentrasi ekstrak dan kematangan daun kersen yang paling efektif dalam menekan pertumbuhan C. gloeosporioides baik in vitro maupun in vivo. Rancangan percobaan pada uji in vitro disusun dalam Rancangan Acak Lengkap tersarang dengan 15 perlakuan dan 3 ulangan.  Ekstrak daun kersen yang digunakan yaitu ekstrak daun muda, tua, dan sangat tua dengan konsentrasi masing-masing yaitu 0%, 15%, 30%, 45%, dan 60%.  Data yang diperoleh diuji lanjut menggunakan uji BNJ dan ortogonal polinomial pada taraf 5%.  Rancangan percobaan yang digunakan pada percobaan in vivo adalah Rancangan Acak Kelompok dengan 4 perlakukan dan 5 ulangan.  Perlakuan tersebut terdiri dari kontrol, ekstrak daun kersen muda 60%, ekstrak daun kersen setengah tua 60%, dan ekstrak daun kersent tua 60%.  Data yang diperoleh diuji lanjut menggunakan BNJ pada taraf 5%.  Hasil penelitian menunjukkan bahwa ekstrak daun kersen pada tingkat kematangan yang berbeda memiliki pengaruh yang sama dalam menghambat pertumbuhan C. gloeosporioides dan semakin tinggi konsentrasi yang digunakan maka semakin efektif dalam menghambat pertumbuhan jamur uji.