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EXPRESSION OF FOUR CYTOKINE/CHEMOKINE GENES IN PERIPHERAL BLOOD MONONUCLEAR CELLS INFECTED WITH DENGUE VIRUS Dewa Ayu Putri Sri Masyeni; Usman Hadi; Kuntaman Kuntaman; Benediktus Yohan; Nur Ita Margyaningsih; R Tedjo Sasmono
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 4 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Overproduction of numerous pro-inflammatory cytokines, during dengue virus (DENV) infection, has been related to plasma leakage in the vascular endothelium and studied elsewhere with conflicting results. The current study objective is to evaluate the expression of four cytokine/chemokine genes following DENV-2 infection within peripheral blood mononuclear cells (PBMC) isolated from a healthy donor. Venous blood was drawn, and PBMCs were isolated using Ficoll density gradient centrifugation. Cells were maintained in culture medium and infected with Indonesian isolate of DENV-2. Cells were harvested and followed by total RNA extraction and reverse-transcription into cDNA using oligo d(T) primers and Reverse Transcriptase enzyme system. The SYBR Green-based quantitative qRT-PCR was used to calculate the relative expression of IL-6, IL-8, IP-10 and MIP-1β- encoding genes during infection time points, compared to uninfected cell controls. The observation of the cytokine was on the 6 and 18 hours post-infection. The different expression profiles of cytokines/chemokines were observed. The up-regulation of gene expression was observed for IL-8 and IP-10. In contrast, the down-regulatory of IL-6 and MIP-1β genes expression was documented during the infection period. The cytokine IL-8 and IP-10 are potent chemoattractants in the recruitment of neutrophil, basophil, and lymphocytes in response to an infection. The highlight of this study is on the up-regulation of IL-8 and IP-10 genes expression which may confirm the roles of these chemokines in the pathogenesis of dengue infection.

Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Benediktus Yohan; Erni Juwita Nelwan; R. Tedjo Sasmono
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.

Persistence of long COVID symptoms in COVID-19 survivors worldwide and its potential pathogenesis - A systematic review and meta-analysis Marhami Fahriani; Muhammad Ilmawan; Jonny K. Fajar; Helnida A. Maliga; Andri Frediansyah; Sri Masyeni; Hanifah Yusuf; Firzan Nainu; Francesco Rosiello; Salin Sirinam; Synat Keam; Youdiil Ophinni
Narra J Vol. 1 No. 2 (2021): August 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i2.36

The study sought to determine the prevalence of persistent long COVID symptoms such as anxiety, depression, dizziness, chest pain, sleep difficulty, palpitations, weight loss, and hair loss among coronavirus disease 2019 (COVID-19) survivors worldwide and to discuss the potential pathogeneses. Potential studies were searched in three databases (PubMed, Scopus, and Web of Science) as of January 30, 2021. Data on study characteristics, patient characteristics during the follow-up, the number of patients with persistent long COVID symptoms and total COVID-19 survivors were collected according to PRISMA guidelines. To assess the quality of studies, the Newcastle-Ottawa scale was used. The estimated prevalence of each long COVID symptom and the association between COVID-19 severity and the occurrence of prolonged symptoms was assessed, if appropriate. The global prevalence of prolonged anxiety was 15.76% (95%CI: 6.36%, 25.15%). Chest pain persisted in 10.36% (239/3,224) of COVID-19 patients (95%CI: 4.92%, 15.80%). Prolonged depression was found in 24 of 548 COVID-19 survivors with an estimated prevalence of 4.32% (95%CI: 2.62%, 6.03%) and dizziness was presented in 4.83% (118/2,219, 95%CI: 1.50%, 8.16%) after recovery. Hair loss was complained by 527 of 2,251 recovered patients (cumulative prevalence of 24.76%, 95%CI: 19.60%, 29.91%), while weight loss was identified in 37 cases among 452 COVID-19 survivors (8.19%, 95%CI: 5.66%, 10.71%). Prolonged palpitation was experienced by 19.38% (211/1,926) survivors with 95%CI: 2.40%, 41.16%. Sleep difficulty was found in 541 of 2,622 COVID-19 survivors (17.87%, 95%CI: 7.55%, 28.20%). The association between COVID-19 severity and the occurrence of persistent long COVID symptoms was not analyzed due to the lack of data. In conclusion, persistent psychological symptoms are frequently reported among COVID-19 survivors. Follow-up studies with a longer duration and larger population are warranted to assess the extent of prolonged symptoms and the quality of life of COVID-19 survivors. Despite various potential pathogeneses that have been hypothesized, a definitive mechanism is yet to be addressed.

Titer disparity of anti-Spike receptor binding domain SARS-CoV-2 antibody between vaccinated and naturally infected individuals Dewa P. Surawan; Duwi Sumohadi; Anak AG. Budhitresna; Putri P. Lestari; Kartika Dewi; Wasudewa Wikananda; Retenra P. Suwari; Md. Rabiul Islam; Haypheng Te; Ali A. Rabaan; Sri Masyeni
Narra J Vol. 2 No. 1 (2022): April 2022
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v2i1.71

In conjunction with other health promotion strategies, vaccination of coronavirus disease 2019 (COVID-19) is a strategy to alleviate the burden of infection. The aim of this study was to determine the differences in antibody response strength between individuals who received COVID-19 vaccination and those who had a natural infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A cross-sectional study was conducted among post-natural confirmed COVID-19 infection and immunized people in Bali, Indonesia. The vaccination was using Sinovac-CoronaVac with two-weeks interval between the two vaccine doses. To measure the level of anti-Spike receptor binding domain (SRBD) of SARS-CoV-2 antibody, we used Roche electro-chemiluminescence immunoassay (ECLIA) platform. Blood samples were obtained before and 28 days after first immunization in the vaccinated group, as well as two weeks after hospital discharge in the confirmed COVID-19 patients based on real-time reverse transcription polymerase chain reaction (RT-PCR). A total of 58 confirmed COVID-19 patients and 60 vaccinated individuals were included. On the 28th day after the initial vaccination, the seroconversion rate among vaccinated individuals was 91.67%. The mean titer of anti-SRBD SARS-CoV-2 antibody among vaccinated participants was 63.62±82.57 IU/mL (ranged between 0 IU/mL and 250 IU/mL). The mean titer among naturally infected group was 188.47±94.57 IU/mL (ranged between 4.25 IU/mL to 250 IU/mL) regardless the severity of COVID-19. Our data suggested that the titer of anti-SRBD SARS-CoV-2 antibody was significantly higher in naturally infected individuals compared to those who received COVID-19 vaccination (p<0.001). These data suggest that not all individuals vaccinated with Sinovac COVID-19 had protective level of anti-SRBD SARS-CoV-2 antibody and booster dose of heterologous vaccine maybe required.

PEMBERDAYAAN KELOMPOK IBU PKK DI DESA KERTA KECAMATAN PAYANGAN KABUPATEN GIANYAR BALI SEBAGAI PEMBANTU JUMANTIK UNTUK MENCEGAH INFEKSI DEMAM BERDARAH DENGUE Sri Masyeni; A.A.G Indraningrat; P.A Suryanditha; Agus Santosa
WICAKSANA: Jurnal Lingkungan dan Pembangunan Vol. 4 No. 1 (2020)
Publisher : Lembaga Penelitian, Universitas Warmadewa

Demam berdarah dengue (DBD) yang terjadi akibat infeksi virus Dengue merupakan penyakit yang mewabah tiap tahunnya di Bali dan Indonesia pada umumnya. Upaya menekan laju DBD khususnya melalui eradikasi vektor pembawa virus yaitu nyamuk Aedes aegypti perlu terus dilakukan dengan melibatkan peran serta masyarakat secara langsung. Penyuluhan mengenai DBD terhadap kelompok ibu PKK di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar, Provinsi Bali telah dilaksanakan dengan hasil yang memuaskan. Metode yang digunakan adalah presentasi dan diskusi melalui tatap muka. Hasil penyuluhan menunjukkan peningkatan pemahaman ibu-ibu PKK terhadap DBD dan upaya pencegahannya. Sebagai tindak lanjut kegiatan pengabdian ini, tim pembantu Jumantik dari anggota PKK Desa Kerta, Kecamatan Payangan telah dibentuk. Tim ini diharapkan berperan aktif dalam memonitoring dan meminimalisir tempat berkembang biak jentik nyamuk Aedes aegypti, serta secara aktif mensosialisasikan pencegahan DBD di lingkungan tempat tinggal mereka.

PEMBERDAYAAN KELOMPOK MASYARAKAT SEBAGAI PENGAWAS MINUM OBAT PASIEN GAGAL GINJAL KRONIS DI RS SANJIWANI D. G. A Budiyasa; Dewa Nyoman Sadguna; D. A. P Niti Widari; Sri Masyeni
WICAKSANA: Jurnal Lingkungan dan Pembangunan Vol. 5 No. 2 (2021)
Publisher : Lembaga Penelitian, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wicaksana.5.2.2021.65-69

Penyakit ginjal kronis (PGK) merupakan penyakit yang ditandai penurunan fungsi ginjal yang semakin progresif sehingga memerlukan bantuan berupa terapi pengganti mesin dialysis untuk menggantikan fungsi ginjal. Hemodialisis dengan mesin hemodialisis harus dilakukan secara rutin 2-3 kali seminggu dan berkesinambungan seumur hidup pasien. Selain hemodialisis atau cuci darah rutin, pasien PGK juga harus mengonsumsi obat dalam jumlah yang relatif banyak yang apabila tidak dikonsumsi secara rutin akan memicu kondisi akut yang mungkin akan memperburuk fungsi ginjal. Untuk mengantisipasi hal ini diperlukan bantuan keluarga terdekat untuk membantu mengawai pasien PGK untuk selalu mengonsumsi obat-obat sesuai dengan instruksi dokter. Kegiatan PKM ini bertujuan untuk membentuk kelompok Kader Siaga Minum Obat pada mitra yang akan memantau pasien mematuhi instruksi dokter. Kegiatan PKM dimulai dengan sosialisasi kegiatan, focus group discussion, pelaksanaan kegiatan serta evaluasi hasil kegiatan. Adapun outcome dari PKM ini adalah terdapat peningkatan pengetahuan mitra mengenai penyakit PGK, pencegahan perburukan serta peningkatan kualitas hidup pasien PGK sebesar 81% pada delapan mitra PKM. Output sementara, kegiatan dilakukan dengan observasi dan pengamatan frekuensi kejadian perburukan penyakit pada pasien sebagai hasil/peran dari fungsi kader Siaga Minum Obat.

Retrospective Analysis on Antibiotic Resistance among Clinical Bacterial Isolates in the General Hospital Sanjiwani, Gianyar Bali Anak Agung Gede Indraningrat; Dewa Ayu Putri Sri Masyeni; I Made Sila Darmana
Jurnal Kesehatan Indonesia Vol 12 No 3 (2022): Juli 2022
Publisher : HB PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Resistensi antibiotik saat ini merupakan ancaman kesehatan global yang muncul akibat penggunaan antibiotik yang salah dan berlebihan sehingga meningkatkan angka mortalitas dan morbiditas. Informasi tentang resistensi antibiotik sangat penting untuk menentukan pengobatan yang ideal bagi pasien. Ketersedian data terkait resistensi antibiotika di Bali masih cukup rendah. Studi ini bertujuan untuk menganalisi profil kerentanan antibiotik pada isolat bakteri klinik di RSUD Sanjiwani Gianyar Bali menggunakan metode retrospektif tahun 2018. Data tersebut meliputi jenis antibiotik yang diuji, nama isolat bakteri klinis, sumber isolasi. dan profil resistensi antibiotik. Diantara 65 isolat bakteri klinis, ditemukan keragaman bakteri patogen yang tinggi dan dikelompokkan menjadi 22 spesies, dimana Escherichia coli, Klebsiela pneumoniae, Acinetobacter baumanii dan Staphylococcus aureus merupakan empat isolat dominan yang diidentifikasi. Paparan terhadap 17 jenis antibiotika menunjukkan bahwa isolat bakteri dominan resisten terhadap levofloxacin, ciprofloxacin, dan gentamycin. Sedangkan isolat dominan tersebut rentan terhadap meropenem, amikacyin dan ampicillin-sulbactam. Surveilans rutin untuk mengetahui profil resistensi antibiotik penting dilakukan dan direkomendasikan untuk menghasilkan antibiotikogram lokal sebagai pedoman untuk mengatasi infeksi patogen.

Faktor-Faktor yang Berhubungan Dengan Kejadian Proteinuria pada Pasien Diabetes Melitus di Puskesmas I dan IV Denpasar Selatan PUTU ARYA SURYANDITHA; NI MADE DHANISWARA PUTRI WIRAWAN; DEWA AYU PUTRI SRI MASYENI
HANG TUAH MEDICAL JOURNAL Vol 20 No 1 (2022): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v20i1.331

Diabetes Mellitus (DM) is a major health problem in the world. Various complications can arise due to uncontrolled blood sugar levels. One of the complications of DM involving the kidneys is diabetic nephropathy, which is characterized by the occurrence of proteinuria in DM patients. This study aims to determine the factors associated with the incidence of proteinuria in patients with diabetes mellitus at Puskesmas I and IV Denpasar Selatan. The design in this study is an analytical study with a cross sectional approach using primary data obtained from interviews and urinalysis examinations. The minimum sample size in this study was 106 people who met the inclusion and exclusion criteria. Data were analyzed using Chi Square test with a significance level of 5%. The number of respondents in this study were 106 respondents. The results of this study showed that as many as 33% of all DM patients had proteinuria. There is a significant relationship between the onset of DM and the incidence of proteinuria (p<0.05), so it can be interpreted that the longer the duration of experiencing DM, the incidence of proteinuria will increase, and vice versa. There was no significant relationship between age, gender, BMI, type of DM therapy, and fasting blood glucose levels with the incidence of proteinuria (p>0,05). Patients with diabetes onset more than 10 years tend to have proteinuria. DM patients are advised to do regular proteinuria checks if the onset of DM is more than 5 years.

Pemberdayaan Kelompok Ibu PKK di Desa Kerta Kecamatan Payangan Kabupaten Gianyar Bali Sebagai Pembantu Jumantik untuk Mencegah Infeksi Demam Berdarah Dengue DAP Sri Masyeni; AAG Indraningrat; PA Suryandhita; Agus Santosa
Community Service Journal (CSJ) Vol. 3 No. 2 (2021)
Publisher : Lembaga Pengabdian Kepada Masyarakat, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1114.235 KB)

Demam berdarah dengue (DBD) yang terjadi akibat infeksi virus Dengue merupakan penyakit yang mewabah tiap tahunnya di Bali dan Indonesia pada umumnya. Upaya menekan laju DBD khususnya melalui eradikasi vektor pembawa virus yaitu nyamuk Aedes aegypti perlu terus dilakukan dengan melibatkan peran serta masyarakat secara langsung. Penyuluhan mengenai DBD terhadap kelompok ibu PKK di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar, Provinsi Bali telah dilaksanakan dengan hasil yang memuaskan. Metode yang digunakan adalah presentasi dan diskusi melalui tatap muka. Hasil penyuluhan menunjukkan peningkatan pemahaman ibu-ibu PKK terhadap DBD dan upaya pencegahannya. Sebagai tindak lanjut kegiatan pengabdian ini, tim pembantu Jumantik dari anggota PKK Desa Kerta, Kecamatan Payangan telah dibentuk. Tim ini diharapkan berperan aktif dalam memonitoring dan meminimalisir tempat berkembang biak jentik nyamuk Aedes aegypti, serta secara aktif mensosialisasikan pencegahan DBD di lingkungan tempat tinggal mereka.

Pemberdayaan Kelompok Petugas Kebersihan di Pasar Kreneng Denpasar Sebagai Kader Pencegahan Penularan Covid-19 Anak Agung Gede Indraningrat; Made Dharmesti Wijaya; Dewa Ayu Putri Sri Masyeni
Community Service Journal (CSJ) Vol. 2 No. 2 (2020)
Publisher : Lembaga Pengabdian Kepada Masyarakat, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1000.747 KB)

Merebaknya pandemi COVID-19 di awal tahun 2020 memerlukan aksi nyata dalam upaya pencegahannya di masyarakat. Pemerintah telah secara gencar menyosialisasikan perilaku hidup bersih dan sehat (PHBS) yang meliputi: mencuci tangan memakai sabun, memakai masker apabila bepergian, mengikuti etika bersin dan batuk, menjaga asupan makanan bergizi dan melakukan aktivitas fisik yang cukup. Penerapan PHBS ini semakin tinggi urgensinya pada kelompok petugas kebersihan pasar mengingat tingginya intensitas paparan terhadap sampah dan berbagai macam kotoran yang dapat menularkan berbagai macam penyakit. Kegiatan pengabdian kepada masyarakat (PKM) ini difokuskan untuk menyiapkan kader guna menyosialisasikan gerakan PHBS kepada kelompok petugas kebersihan di Pasar Kreneng Denpasar. Pemilihan lokasi dan topik pada PKM ini didasarkan atas masih kurangnya pemahaman akan PHBS di kalangan petugas kebersihan di pasar Kreneng. Kegiatan PKM ini terdiri dari sosialisasi, focus group discussion, pemaparan materi, pre-test dan post-test, yang diakhiri dengan evaluasi dan monitoring secara berkala selama 1 bulan. Hasil kegiatan menunjukkan kader telah memahami dan mempraktekkan konsep PHBS seperti mencuci tangan memakai sabun yang baik dan benar, memakai masker dengan baik dan benar, serta mengonsumsi makanan dengan gizi seimbang. Selain itu pemahaman kader akan konsep PHBS secara signifikan telah ditunjukkan dengan peningkatan nilai post-test dibandingkan pre-test sebesar 40%. Kedepannya diharapkan kader petugas kebersihan dapat secara aktif menyosialisasikan gerakan PHBS pada petugas kebersihan lainnya dan warga di sekitar Pasar Kreneng Denpasar khususnya dalam upaya mencegah penularan COVID-19.

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