Senny Helmiati
Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta Indonesia

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Parasit Anisakis sp. Pada Populasi Layur (Trichiurus sp.) yang Didaratkan di Pelabuhan Ikan Cilacap Suadi Suadi; Senny Helmiati; Retno Widaningroem
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.35

Abstract

The aims of this research were to determine the population parameters of hairtails (Trichiurus sp.) and the potential use of fish parasite (Anisakis sp.) for its stock assessment. Fishery survey was conducted at southern coast of Cilacap, Central Java on September to October 2004. Total length and weight of 527 fish samples were measured in five periods during the field survey and 30 samples were collected randomly for further observation to analyze the Anasakis sp., sex ratio, and gonad maturity. The average length of catched fish was ranged between 62  to 70 cm and weight was 200  to 250 g. The  length-weight (L-W) relationship indicated positive allometric growth (b=3.6076). Sex ratio was balance at 1:1 with gonad maturity range between I to IV for female and I to III for male. Anisakis sp. were found as endoparasite and potentially used as biological tags for fish stock assessment such as migration pattern and feeding habit.
Infeksi Anisakis sp. pada Layur (Trichiurus sp. ) di Pantai Selatan Kabupaten Purworejo Eko Setyobudi; Senny Helmiati; Soeparno Soeparno
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.73

Abstract

The aims of this research were to know the prevalency, intensity and distribution of Anisakis sp. which infected hairtail (Trichiurus sp.) in the southern coast of Purworejo Regency. Samples were collected from fish landing-places in Jatimalang and Keburuhan, Purworejo Regency. Totally 276 fish were collected as samples during 4 months (September-December 2005). Every sampel was measured in total length and body weight. The examination of Anisakis sp. were conducted on body surface, body cavity, gut, liver, gonad, and muscle. Results of the research indicated that the Anisakis sp. infected various size of hairtail, with prevalency 62,68% and mean intensity of infection 3,30 individual/fish. Infected organs were body cavities (peritoneum), guts, gonads, livers, muscles, gills and pneumatocysts.
PENGGUNAAN TEPUNG KULIT BUAH KAKAO PADA PAKAN PENGARUHNYA TERHADAP PERTUMBUHAN DAN PRODUKSI GURAMI Sukardi Sukardi; Senny Helmiati; Yunita Wulandari
Jurnal Perikanan Universitas Gadjah Mada Vol 14, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9112

Abstract

The aims of the research were to observe (1) utilization of cocoa pod husk meal to fish feed especially for carbohydrate source (2) effect of substitution rice bran by cocoa pod husk meal on growth and product of giant goramy (3) find the substitution doses of rice bran by cocoa pod husk meal on feed which could give better influence on giant goramy growth. The research was done for 8 months in Fisheries Research Station Laboratory, Agriculture Faculty Gadjah Mada University. Research was conduct by cultured of giant goramy on fibre tank, for 60 days. There were 5 treatmens substitution dose (20, 40, 60, and 80%) and control (comercial feed) with 3 replications. During cultured the tank were irrigated and the fish were fed 3% of body weight 3 times a day. The result showed that cocoa pod husk meal can substitute rice bran on giant goramy feed. Substitution rice bran by cocoa pod husk meal on fed not significantly affect on the growth, product and survival rate of giant goramy, but significant affected on buoyancy of feed. Giant goramy were feeding by comercial feed had growth and product better than feeding by artificial feed.
Pengaruh Ketebalan Media Budidaya Cacing Sutra (Tubifex sp.) Menggunakan Lumpur Limbah Budidaya Lele Dindin Suryadin; Senny Helmiati; Rustadi Rustadi
Jurnal Perikanan Universitas Gadjah Mada Vol 19, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.26015

Abstract

This research aims to know the influence of the thickness of catfish culture waste on silk worm (Tubifex sp.) biomass. The research was conducted with culturing Tubifex sp. in different thickness media of the waste as treatments. The treatments consist of the waste 2, 4, 8 and 12 cm thickness of medium with 6 cm depth. The stock densities 150 g/m3 with average weight 0.0062+0.00032 g. The parameter that analyzed is biomass and population of Tubifex sp.. Data analyzed by analysis of variance and posthoc test is Duncan’s Multiple Range Test. The result shows that diversification of medium thickness gives the real influence (P<0.05) to Tubifex sp. biomass. The highest biomass 1745.14±143.77 g/m3 and population 233,848.46±19265.61 ind./m3 on the treatment with 8 cm thickness. The relationship between treatment and biomass production of Tubifex sp. is quadratic relationship with y = -30.405x2 + 552.18x – 823.55. The optimum thickness of medium that results maximum biomass production is 9.1 cm.
Toxicity Test for Evaluating Food Safety of New Edible Seaweeds, Enteromorpha sp. and Laurencia sp. Alim Isnansetyo; Indah Istiqomah; Retno Widaningroem; Triyanto Triyanto; Rina Anggraeni Safia; Riesa Yosita; Senny Helmiati
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.06 KB) | DOI: 10.22146/jfs.34103

Abstract

Seaweeds are potential foodstuff and source of bioactive substances that are very useful in the food and pharmaceutical industries. The objective of this study was to evaluate toxicity of aqueous extracts of seaweeds by Brine shrimp lethality test (BLT) and cytotoxicity test.  The seaweeds used in this study were Enteromorpha sp. and Laurencia sp. collected from the Gunungkidul coastal line. The cytotoxicity test was performed against a normal cells line of Vero cells and a cancer cells line of HeLa cells. The cells lines were cultured in M119 medium with addition of 10% FBS and incubated at 37°C in CO2 incubator until confluent. Cells were harvested and cultured in 96 microplate with an initial density of 2x104 cells / 100 ml / well.  The cells lines were treated with seaweed extracts at 24 hours after incubation. After additional 24 h incubation, the cell proliferation was observed by MTT method (3- [4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide.The results showed that the two aqueous extract were not toxic in the BLT test, and did not exhibited any cytotoxicity activity against HeLa and Vero cells lines. Concentration-dependent cell proliferation was also not found. This results indicated that Enteromorpha sp.  and Laurencia sp. extracts were no cytotoxic and might be consumed safely. These overall results suggested that the two seaweeds might be developed to be a new foodstuff  for humans.
Evaluasi Kandungan Nutrien dan Antinutrien Tepung Daun Kelor Terfermentasi sebagai Bahan Baku Pakan Ikan Senny Helmiati; Rustadi Rustadi; Alim Isnansetyo; Zulprizal Zulprizal
Jurnal Perikanan Universitas Gadjah Mada Vol 22, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.58526

Abstract

Penelitian ini bertujuan untuk mengevaluasi kandungan nutrien dan antinutrien tepung daun kelor terfermentasi sebagai sumber protein nabati pakan ikan. Tahap penelitian meliputi pembuatan tepung daun kelor, persiapan dan penghitungan kepadatan bakteri, proses fermentasi, dan analisis kandungan nutrien dan antinutriennya. Bakteri yang digunakan untuk fermentasi merupakan campuran bakteri T2A (Bacillus sp.), T3P1 (Bacillus sp.) dan JAL11 (Lactococcus raffinolactis) dengan kepadatan sebesar 2,16x109 cfu/mL. Fermentasi tepung daun kelor dilakukan selama 168 jam. Analisis kandungan nutrien dan zat antinutrien tepung daun kelor terfermentasi dilakukan pada jam ke-24, ke-48, ke-72, ke-96, ke-120, ke-144 dan ke-168. Kandungan nutrien tepung daun kelor meliputi kadar air (9,04±0,00%), abu (9,70±0,21%), protein (25,77±0,08%), lemak (4,80±0,52%), serat kasar (11,60±0,13%), bahan ekstrak tanpa nitrogen (39,06±0,52%), energi (351,27±3,27 kkal/00 g), hemiselulosa (13,79±0,07%), selulosa (9,9±0,06%) dan lignin (15,34±0,31%). Fermentasi dapat meningkatkan kadar air, abu, protein dan lemak, serta menurunkan kadar serat kasar, bahan ekstrak tanpa nitrogen, hemiselulosa, selulosa, lignin dan antinutrien, antara lain fenol, tanin, asam fitat dan HCN. Hasil penelitian menunjukkan bahwa fermentasi dapat meningkatkan kandungan nutrien dan menurunkan kandungan antinutrien tepung daun kelor, sehingga dapat digunakan sebagai sumber protein nabati pada bahan baku pakan ikan.