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Journal : The Journal of Experimental Life Sciences (JELS)

The Use of Full-Grained, Fragmented, and Reduced Marine Yeast Powder in Fodder Formulation to Improve the Growth of Eel Fish (Anguilla bicolor) Ria Retno Dewi Sartika Manik; Arning Wilujeng Ekawati; Hardoko Hardoko
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.126 KB) | DOI: 10.21776/ub.jels.2016.006.02.05

Abstract

Ikan Sidat or eel fish (Anguilla bicolor) is a popular fish commodity in domestic trade as well as for export commodity. The use of marine yeast as fodder suffers from some weaknesses since it contains nucleic acids and rigid thick cell walls that obstruct the absorbance of nutrition. Optimizing the use of marine yeast as fodder ingredients can be done by fragmenting and reducing the yeast. This research attempts at optimizing the use of marine yeast regarding its rate of growth, fodder efficiency and the survival rate of eel fish. This research employs the complete random design using 4 different treatments and 3 repetition. In the four treatments, the fodder contained protein (45%) and iso energy (3.600 kkal.kg-1 fodder), which were applied to the treatment A 0% (control), B 5% (complete), C 5% (fragmented) and D 5% (reduced). The parameters used in this study were survival rate, specific growth rate, ratio of fodder conversion, ratio of protein efficiency, energy retention, protein retention and rate of metabolism. The data of this study showed the best result in the treatment D, 5% (reduced), showing survival rate value of 95.56%, specific growth at 1.12, food conversion ratio of 2.27, protein efficiency ratio of 0.98, protein retention at 18.26%, energy retention of 16.57, and protein metabolism rate at 81.96%. Keywords: eel fish, growth, marine yeast.
Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum Hardoko Hardoko; Bambang B. Sasmito; Yunita E. Puspitasari; Hamid M. Afandi; Nugroho Maulia
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1225.92 KB) | DOI: 10.21776/ub.jels.2017.007.02.10

Abstract

Shrimp shells are one of chitin sources in Indonesia which is potential to be converted into multifunctional glucosamine. This research was aimed to study the glucosamine production by fermentation using Trichoderma harzianum.  Method used was experimental fermentation with pH treatment of 3-5 and fermentation duration of 10-20 days, and designed using Response Surface Methodology (RSM). Results showed that fermentation duration of 10-20 days has reached the optimum point of glucosamine production from shrimp shells using T. harzianum. The highest production of D-glucosamine in fermentation using T. harzianum occurred on initial pH of 5.41 and fermentation duration of 15 days (18,294.95 ppm), while the highest N-Acethyl-D-Glucosamine production occurred on initial pH of 3.00 and fermentation duration of 20 days (127,000.00 ppm).Keywords: fermentation, glucosamine, shrimp shells, T. harzianum.