Article Per Year (5 Year)
p-Index From 2019 - 2024
1.683
P-Index
This Author published in this journals
All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Perikanan dan Kelautan The Journal of Experimental Life Sciences (JELS) Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Sumberdaya Akuatik Indopasifik Jurnal Perikanan Universitas Gadjah Mada Khadimul Ummah Journal of Social Dedication Indonesian Food Science and TechnologyJournal FaST : Jurnal Sains dan Teknologi Asian Journal of Aquatic Sciences
Hardoko Hardoko
Fakultas Perikanan Universitas Brawijaya Malang.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
PEMBUATAN MI SINGKONG : KARAKTERISASI MI SINGKONG HASIL PENAMBAHAN JENIS PROTEIN DAN RASIO TEPUNG SINGKONG TERHADAP TAPIOKA Hardoko Hardoko; Clara Tasia; Titri Siratantri Mastuti
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol 5, No 1 (2021): MAY
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cassava noodles are noodles made from cassava and do not contain gluten (non gluten). Non-gluten-based noodles are less attractive to the public because of the physical properties of the noodles. The purpose of this study was to obtain the appropriate type of protein and the ratio of cassava flour to tapioca in the manufacture of cassava noodles. The method used is an experimental method which is divided into two stages. The first stage of the research was made treatment of soy protein isolate (ISP) 5%, ISP 10%, eggs 2.5%, 5%, and 7.5%. The second stage was treated with the ratio of cassava flour - tapioca ((80:20, 70:30, 60:40, 50:50) and the best types of protein were stage one (X), X-1.5%, and X + 1.5%. that the addition of 5% eggs can reduce the value of cooking losses, stickiness level, and increase the water absorption and the elasticity of the noodles The results of the sensory comparison test with commercial noodles show that cassava noodles are more chewy and stickier than commercial noodles but have no taste and aroma of cassava. In the comparison test between cassava noodles and commercial noodles, the value is acceptable. The results of the second stage of the study showed that the best noodles were the best cassava noodles from the treatment of the ratio of 60:40 cassava flour: tapioca and the addition of eggs of 6.5%. This treatment resulted in shrunken cassava noodles. lower cooking and stickiness, high water absorption, high elasticity, and sensoy hedonic grades are somewhat preferred.
Studi Penurunan Glukosa Darah Diabet dengan Konsumsi Rumput Laut Eucheuma cottonii Hardoko Hardoko
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.70

Abstract

The purpose of this study was to observe the effect of Eucheuma cottonii consumption to the diabetic blood glucose level. Study on reducing blood glucose by consuming seaweed E. cottonii was conducted using male wistar rat (Ratus norvegicus). Rats were made to a hyperglycemic condition by aloxan injection before given ransom contains of 5, 10, 15, 20% (w/w) E. cottonii, standard ransom (negative control), and parental glibenklamid (positive control). The result showed that standard ransom was not able to reduce blood glucose from hyperglycemic to normal level, while ransoms with E. cottonii were able to do so. The higher E. cottonii seaweed level in the ransom had the higher capacity to decrease blood glucose level. The ransom with 20 and 15% E. cottonii were able to reduce blood glucose in 18 and 21 days, respectively.This treatment has the same ability as glibenklamid medicine which also reduced blood glucose to normal level in 18 days. Ransom with 5 and 10% E. cottonii were also able to reduce blood glucose level but the normal blood glucose level could not be achieved until 21st day.
Determination of Optimal Fermentation Condition for N-acetylglucosamine Production Using Mucor circinelloides Extracellular Chitinase Yuniwaty Halim; Hardoko Hardoko; Reinald Febryanto Pengalila
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.742 KB) | DOI: 10.22146/jfs.45859

Abstract

This research aimed to determine the best fermentation condition, consists of pH, temperature, fermentation time and substrate concentration, in N-acetylglucosamine production from shrimp shells using crude extracellular chitinase obtained from Mucor circinelloides mould. The method used was experimental method with fermentation treatment of different pH (5, 6, 7, 8 and 9) and temperature (30, 40, 50, 60, 70 and 80°C). The optimal pH and temperature of fermentation obtained was used to determine the maximum substrate concentration (0.5, 1, 1.5 and 2%) and fermentation time (2, 4, 6 and 24 hours) to produce the highest concentration of N-acetylglucosamine. The optimal pH for fermentation was 8, with chitinase activity of 4.38±0.06 U/ml, while the optimal temperature was 50°C with enzyme activity of 5.42±0.06 U/ml. Substrate concentration and fermentation time affected the N-acetylglucosamine production. The optimal fermentation condition was obtained with substrate concentration of 1.5% and fermentation time of 2 hours resulted to N-acetyl Glucosamine concentration of 2195.83±15.14 ppm.
Identifikasi Senyawa Fitokimia dari Daun Mangrove Sonneratia alba dan Analisis In Silico Sebagai Antidiabetes Yunita Eka Puspitasari; Hardoko Hardoko; Titik Dwi Sulistiyati; Alifah Nur Fajrin; Hezkiel Oktorully Tampubolon
Jurnal Perikanan dan Kelautan Vol 27, No 2 (2022): June 2022
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.241-248

Abstract

Mangrove plant, Sonneratia alba, can be found abundantly in Indonesia. Some previous studies reported phytochemical screening and bioactivity test of Sonneratia sp. as anti-diabetic (in vitro and in vivo analysis). However, phytochemical identification of S. alba leaves and inhibitory activity of α-glucosidase as anti-diabetic have not been reported. The purpose of this study are to identify phytochemical compounds of methanolic extracts S. alba leaves, and to predict inhibitory mechanisms of S. alba leaves against α-glucosidase through in silico analysis. In this study, the research method consists of two steps namely identification phytochemical compounds of methanolic extracts S. alba leaves, and prediction inhibitory activities of phytochemical compounds of methanolic extracts S. alba leaves against α-glucosidase by molecular docking (in silico) analysis. Methanolic extracts S. alba leaves contained orientin, vitexin, luteolin, oleanolic acid and reserpine. Reserpine was identified for the first time in S. alba leaves. Based on in silico analysis, binding energy of orientin, vitexin, luteolin, oleanolic acid, reserpine and acarbose (positive control) against α-glucosidase were -9,7; -9,7; -9,2; -8,6; -10,0 dan -8,3 kcal/mol. This result indicated that compounds inhibited α-glucosidase activities and can be considered as antidiabetic agent.
Correlation Between Bitterness Removal And Functional Properties Of Papaya (Carica Papaya L.) Leaves Natania Kam; Jacinta Fanya Setiadi; Hardoko Hardoko; Dela Rosa
Indonesian Food Science & Technology Journal Vol. 5 No. 2 (2022): Vol 5 No 2, July 2022
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The mature papaya leaves are known for its medicinal properties albeit its distinctive bitter taste. In this research 3 different adsorbents; bentonite, activated attapulgite and diatomaceous earth were used to reduce the bitterness of the papaya leaves. The adsorbent treated papaya leaves were then analysed for its sensory evaluation in terms of bitterness intensity and phytochemical compounds which include phenolic, flavonoid, tannin and alkaloid compounds and also antioxidant activity. Papaya leaves treated with bentonite had the most reduced bitterness and application of heat treatment further enhance the efficacy of bitterness removal. However the removal of the bitterness also slightly affected the phytochemical compounds and their functional properties. The leaves treated with bentonite by method of boiling was found to be the most effective method in reducing bitterness, with bitterness intensity score of 0.3 out of the original 11.2, while retaining its phytochemical compound; total phenolic content of 10.699 mg GAE/g extract, total flavonoid content of 1.468 mg QE/g extract, total tannin content of 9.423 mg TAE/g extract, total alkaloid content of 1.363% while retained most of its Antioxidant IC50 of 1001.058 ppm and Antidiabetic properties of 642.231 ppm
IN SILICO STUDY OF TRITERPENOID IDENTIFIED FROM Ceriops decandra LEAVES AS INHIBITORS OF α-AMYLASE Yunita Eka Puspitasari; Jeny Ernawati Tambunan; Hardoko Hardoko
Asian Journal of Aquatic Sciences Vol 5 No 3 (2022): December
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.5.3.429-439

Abstract

α-amylase has a pivotal role in catalyzing the cleavage of α-1,4-glycosidic bonds of polysaccharides to produce oligosaccharides. The inhibition of α-amylase delays the breakdown of carbohydrates, causing a reduction of blood glucose level absorption in diabetes patients. The exploration of α-amylase inhibitors has attracted because society assumed that utilizing herbal medicine reduced the side effect of prescribed drugs. Mangrove from genus Ceriops have been used as antidiabetic, but the mechanism as α-amylase inhibitors has not been reported. Consumption of leaves extract of C.decandra reduced blood glucose levels in diabetic rats, and triterpenoids have been identified from the leaves. With this in mind, this study aims to predict the molecular interactions between α-amylase (PDB ID: 4GQR) and the inhibitors, triterpenoid identified in C.decandra leaves, and to evaluate the potency of triterpenoid as α-amylase inhibitor. There are five triterpenoids identified in C.decandra leaves used as ligand tests, including lupenone, betulin, betulonic acid, betulinic acid, and lupeol. The descriptive method was applied in this investigation. This study was carried out from June to September 2022. Based on the molecular interactions, the binding affinity of triterpenoids was lower than the native ligand and control ligand. Lupenone, lupeol, betalonic acid, and betulinic acid inhibited α-amylase activity by non-competitive inhibition. It was predicted that betulin inhibited α-amylase activity through competitive inhibition
Analisis Bilangan Peroksida, Organoleptik, dan Proksimat Abon Tuna dengan Fortifikasi Jantung Pisang Heder Djamaludin; Hardoko Hardoko; Muhammad Dailami; Vivi Nurhadianty; Delvira Riska Ananta; Dean Rama Prayoga
Jurnal Sumberdaya Akuatik Indopasifik Vol 6 No 4 (2022): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2022.Vol.6.No.4.250

Abstract

Fish is part of the diet which is a source of animal protein which is very beneficial for health. To meet the availability of fish-based food, it can be processed into various processed products, one of which is shredded fish. Shredded fish still has a drawback, such as the low fiber content. To enrich the fiber content of shredded fish, fortification with banana blossoms can be an alternative. The process of making shredded fish does not yet have a standard because many methods and spices are added, so there are variations in the types and amounts of spices used. This causes the quality of shredded fish to vary. Therefore, this study aimed to evaluate the chemical properties of the effect of different concentrations on banana blossoms fortification in the processing of shredded tuna. The research was conducted in June-August 2022 at the Fish Product Technology Laboratory and the Chemical Engineering Laboratory, Universitas Brawijaya. The study design was a non-factorial Completely Randomized Design (CRD). The results were analyzed using ANOVA (α 5%) and Tukey's post hoc test. The results showed that banana blossoms fortification had no significant effect on the peroxide value and water content of shredded tuna. Banana blossoms fortification has a significant effect on the hedonic value of the appearance, taste, and texture of shredded tuna. However, it has no significant effect on the hedonic aroma value and overall acceptance. The value of the proximate composition of shredded tuna with the best treatment level was 70%, i.e. protein content of 14.51%, fat content of 36.19%, ash content of 6.82%, carbohydrate content of 29.15%, and total dietary fiber of 20.80%. The fat and protein content of shredded tuna does not meet the Indonesian National Standard (SNI) for shredded products.
The Use of Full-Grained, Fragmented, and Reduced Marine Yeast Powder in Fodder Formulation to Improve the Growth of Eel Fish (Anguilla bicolor) Ria Retno Dewi Sartika Manik; Arning Wilujeng Ekawati; Hardoko Hardoko
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.126 KB) | DOI: 10.21776/ub.jels.2016.006.02.05

Abstract

Ikan Sidat or eel fish (Anguilla bicolor) is a popular fish commodity in domestic trade as well as for export commodity. The use of marine yeast as fodder suffers from some weaknesses since it contains nucleic acids and rigid thick cell walls that obstruct the absorbance of nutrition. Optimizing the use of marine yeast as fodder ingredients can be done by fragmenting and reducing the yeast. This research attempts at optimizing the use of marine yeast regarding its rate of growth, fodder efficiency and the survival rate of eel fish. This research employs the complete random design using 4 different treatments and 3 repetition. In the four treatments, the fodder contained protein (45%) and iso energy (3.600 kkal.kg-1 fodder), which were applied to the treatment A 0% (control), B 5% (complete), C 5% (fragmented) and D 5% (reduced). The parameters used in this study were survival rate, specific growth rate, ratio of fodder conversion, ratio of protein efficiency, energy retention, protein retention and rate of metabolism. The data of this study showed the best result in the treatment D, 5% (reduced), showing survival rate value of 95.56%, specific growth at 1.12, food conversion ratio of 2.27, protein efficiency ratio of 0.98, protein retention at 18.26%, energy retention of 16.57, and protein metabolism rate at 81.96%. Keywords: eel fish, growth, marine yeast.
Peningkatan Pengetahuan dan Kesadaran Karyawan Terhadap Good Manufacturing Practices (GMP) dan Sanitasi Makanan pada Produsen Bakso XYZ di Kota Malang Tian Nur Ma'rifat; Eddy Suprayitno; Bambang Budi Sasmita; Titik Dwi Sulistiati; Hardoko Hardoko; Anies Chamidah; Mikchaell Alfanov Pardamean Panjaitan; Heder Djamaluddin; Jenny Ernawati Tambunan
Khadimul Ummah Vol 6, No 1 (2022): November 2022
Publisher : Universitas Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/ku.v6i1.8855

Abstract

Masalah yang dihadapi mitra adalah berkurangnya pendapatan selama pandemi COVID-19. Kemampuan beli masyarakat menurun drastis sehingga beberapa gerai Bakso XYZ tutup. Untuk mengatasi masalah ini, fokus bisnis Bakso XYZ diubah dari direct selling melalui dine-in outlet menjadi penyediaan produk kemasan dari perusahaan. Kendala dalam memasarkan produk ritel kemasannya adalah belum adanya izin edar dari BPOM terkait penerapan Good Manufacturing Practices (GMP) dan sanitasi makanan. Tujuan dari program ini adalah untuk mengetahui pengaruh pelatihan tentang GMP dan sanitasi terhadap peningkatan pengetahuan dan kesadaran karyawan agar Bakso XYZ dapat memiliki izin edar BPOM. Metode yang digunakan untuk mengatasi masalah tersebut adalah evaluasi capaian pengetahuan dan kesadaran terkait GMP yang diterjemahkan ke dalam CPPOB (Cara Produksi Pangan Olahan yang Baik) dan sanitasi makanan kepada karyawan menggunakan metode kuesioner. Sebelum dan sesudah penyuluhan, dilakukan penyebaran kuesioner kepada karyawan untuk melihat apakah kesadaran karyawan tersebut meningkat atau tidak. Hasilnya adalah adanya peningkatan pada aspek pengetahuan tentang pentingnya GMP dengan gap sebesar 0,25, kesadaran tentang prinsip penerapan GMP dengan gap sebesar 0,5  dan pengetahuan tentang pencemaran makanan dengan gap sebesar 0,5 dari skala 5. Dari hasil uji kelayakan fisik mengenai implementasi GMP pada saat sebelum dan sesudah pelatihan, diperoleh hasil bahwa lokasi, bangunan, fasilitas pengolahan pangan dan sanitasi telah tersedia dengan kondisi yang sesuai. Perbaikan terdapat pada perubahan perilaku sanitasi karyawan pada aspek mencuci tangan hingga bersih, memotong kuku hingga pendek, bebas kosmetik dan perilaku yang higenis
PENGHAMBATAN α-GLUCOSIDASE OLEH FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN MANGROVE Sonneratia alba SEBAGAI ANTIDIABETES MELALUI PENAMBATAN MOLEKULER Yunita Eka Puspitasari; Hezkiel Oktorully Tampubolon; Alifiah Nur Fajrin; Titik Dwi Sulistiyati; Hardoko Hardoko
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 1 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.1.%p

Abstract

α-glucosidase memiliki peran penting dalam metabolisme karbohidrat pada tubuh manusia dengan bekerja memutus rantai oligosakarida menjadi gula sederhana seperti monosakarida.Penghambatan aktivitas α-glucosidase menjadi salah satu mekanisme untuk mengendalikan kadar glukosa dalam darah penderita diabetes. Obat herbal sebagai inhibitor α-glucosidase bersumber dari bahan alami laut khususnya mangrove menarik perhatian. Buah pedada Sonneratia alba telah diketahui mengandung senyawa fitokimia yang berpotensi sebagai antidiabetes, akan tetapi belum pernah dilaporkan kandungan senyawa bioaktif yang berperan sebagai inhibitor α-glucosidase. Kelimpahan daun pada tanaman mangrove lebih tinggi daripada buah sehingga pada penelitian ini menggunakan daun sebagai penghambat aktivitas α-glucosidase. Tujuan dari penelitian ini adalah untuk menganalisa profil senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba, dan untuk menentukan senyawa fitokimia tersebut yang berperan sebagai inhibitor α-glucosidase secara in silico melalui penambatan molekuler. Hasil penelitian menunjukkan tiga senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba seperti luteolin, apigenin, dan diosmetin telah diidentifikasi. Menurut kaidah Lipinski, analisa druglikeness dan toksisitas, maka semua senyawa tersebut aman untuk dikonsumsi, Hasil penambatan molekuler menunjukkan energi ikatan yang dihasilkan dari interaksi reseptor dan ligan uji lebih rendah daripada energi ikatan dari reseptor dan ligan kontrol maupun alami. Sehingga dapat disimpulkan bahwa luteolin, apigenin dan diosmetin dari ekstrak etil asetat daun pedada berpotensi sebagai inhibitor α-glucosidase. α-glucosidase plays an important role in carbohydrate metabolism in the human body by breakdown oligosaccharides into monosaccharides. The inhibition of α-glucosidase activity is one of the mechanisms for controlling blood glucose levels in diabetes patients. Herbal drug as an α-glucosidase inhibitor from marine resources, especially mangroves is attracting interest. The fruit of Sonneratia alba contains phytochemical compounds potent as antidiabetic but no report related to their bioactive compound as inhibitor α-glucosidase. The abundance of leaves is higher than the fruit; therefore, in this study, the leaves will be used as α-glucosidase inhibitors. This study aimed to profile bioactive compounds of ethyl acetate extract of pedada S. alba leaves and to evaluate phytochemical compounds of S. alba leaves as α-glucosidase inhibitors using molecular docking in silico. The results showed that three phytochemical compounds of ethyl acetate extract of S. alba leaves, such as luteolin, apigenin, and diosmetin have been identified. According to the Lipinski’s rule, drug likeness, and toxicity test, all compounds are safe to consume. Docking studies showed that binding energy between receptor and phytochemical compounds is lower than binding energy between receptor and ligand control. In conclusion, luteolin, apigenin and diosmetin of ethyl acetate extract of pedada S. alba leaves have potent as α-glucosidase inhibitors.
Co-Authors Alifah Nur Fajrin Alifiah Nur Fajrin Anies Chamidah Arning Wilujeng Ekawati Bambang B. Sasmito Bambang Budi Sasmita Clara Tasia Dean Rama Prayoga Dela Rosa Delvira Riska Ananta Eddy Suprayitno Halim, Yuniwaty Hamid M. Afandi Handayani, Ratna Heder Djamaluddin Heder Djamaludin Hezkiel Oktorully Tampubolon Hezkiel Oktorully Tampubolon Jacinta Fanya Setiadi Jenny Ernawati Tambunan manik, ria retno Mikchaell Alfanov Pardamean Panjaitan Mochamad Arega Alfikri Muhammad Dailami Natania Kam Nugroho Maulia Reinald Febryanto Pengalila Romasni Sitanggang Shella Trey Lamtoro Tambunan, Jeny Ernawati Tian Nur Ma’rifat Titik Dwi Sulistiati Titik Dwi Sulistiyati Titri Siratantri Mastuti Vivi Nurhadianty Yunita E. Puspitasari Yunita Eka Puspitasari