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All Journal BALI MEDICAL JOURNAL (BMJ) Medical Journal of Indonesia The Indonesian Biomedical Journal Universa Medicina Paediatrica Indonesiana SCRIPTA SCORE Scientific Medical Journal Jurnal Ilmu Bedah Indonesia
Eka J. Wahjoepramono
Department Of Neurosurgery, Faculty Of Medicine, Universitas Pelita Harapan, Neuroscience Center, Siloam Hospital Lippo Village, Tangerang, Banten
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : The Indonesian Biomedical Journal

 1 
Dynamic Pattern of Interleukin-1beta and -10 in Cerebrospinal Fluid following Aneurysmal Subarachnoid Hemorrhage Yesaya Yunus; Julius July; Andi Asadul Islam; Eka Julianta Wahjoepramono; Irawan Yusuf
The Indonesian Biomedical Journal Vol 6, No 2 (2014)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v6i2.35

Abstract

BACKGROUND: Inflammation following aneurysmal subarachnoid hemorrhage (SAH) is associated with patient outcome. This study aimed to analyze the dynamics pattern of the pro-inflammatory interleukin (IL)-1β and anti-inflammatory cytokine IL-10 in the cerebrospinal fluid (CSF) following aneurysmal SAH.METHODS: This is a prospective observational study. The CSF was collected prior to surgery, and on the 2nd, 4th and 6th days after surgery. The CSF was then analyzed for IL-1β and -10 using enzyme-linked immunosorbent assay. Delayed Ischemic Neurological Deficits (DIND) was determined based on new neurological deficits within the first week after surgery. Data was analysed with T test or Wilcoxon Rank-Sum test to evaluate the pattern of biomarkers between DIND and non-DIND groups.RESULTS: There were 33 patients enrolled in this study, 16 patients (48.48%) who experienced DIND and 17 patients (51.52%) were non-DIND. There were significant increase dynamic levels of IL-10 and -1β in DIND patients (p<0.05).CONCLUSION: Significant increase levels of IL-10 and -1β in CSF after aneurysmal SAH are associated with DIND.KEYWORDS: IL-1β, IL-10, DIND
Isolation of Mesenchymal Stem Cells from Adipose Tissue Harsan Harsan; Silmi Mariya; Andi Asadul Islam; Eka Julianta Wahjoepramono; Irawan Yusuf
The Indonesian Biomedical Journal Vol 7, No 3 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i3.181

Abstract

BACKGROUND: In searching for the best source of stem cells, researcher found adipose stem cells as one of the ideal source due to its easiness in harvesting and its potential for differentiating into other cell lineage.METHODS: We isolated stem cells from adipose tissue, cultured and confirmed its immunophenotype using polymerase chain reaction.RESULTS: Cluster of differentiation (CD)44, CD73, CD90, CD105 were expressed, which represent immunophenotype of mesenchymal stem cells. CONCLUSION: Mesenchymal stem cells can be isolated from adipose tissue. KEYWORDS: adipose, mesenchymal stem cells, isolation, immunophenotype
Co-Authors Alexander Cahyadi, Alexander Amiruddin Aliah, Amiruddin Andi Asadul Islam Andi Asadul Islam Andi Asadul Islam As'ad, Suryani Beny Atmadja Wirjomartani Budhianto Suhadi Cahyono Kaelan, Cahyono Gilbert Sterling Octavius Harsan Harsan Harsan Ilham J. Patellongi, Ilham J. Ilhamjaya Patellongi Irawan Yusuf Irawan Yusuf Jan S. Purba, Jan S. Julius July Made Agus Mahendra Inggas, Made Agus Mahendra Nicholas Gabriel H.R. Rocksy F.V. Situmeang, Rocksy F.V. Silmi Mariya Sri Maliawan Sutisna Himawan Syarifuddin Wahid THEO AUDI YANTO, THEO AUDI Vivien Puspitasari, Vivien Yesaya Yunus