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All Journal Tropical Medicine Journal The Indonesian Biomedical Journal Jurnal Respirologi Indonesia Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Green Medical Journal Jurnal Keperawatan Klinis dan Komunitas (Clinical and Community Nursing Journal) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia

Widya Wasityastuti

Department Of Physiology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Indonesia

Author-ID : 413248

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Articles

Antifibrotics and Antioxidants of Chlorogenic Acid Inhibits Toll- Like Receptors-4 as Liver Fibrotic Marker Naibey, Rosdiana; Wasityastuti, Widya; Anggorowati, Nungki; Arfian, Nur
Green Medical Journal Vol 3 No 2 August (2021): Green Medical Journal
Publisher : Fakultas Kedokteran Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/gmj.v3i2.86

Introduction: Chlorogenic Acid (CGA) is an antifibrotic and antioxidant for fibrotic tissues. These double roles be able to inhibit or fibrotic tissues chains because of internal and external issues. For example, virus, bacteria or other pathogens and also by drugs, alcohol, cigarettes, etc. as external factor that affect quality of body tissues. Toll-Like Receptor-4 (TLR-4) as a marker fibrotic tissues. It is a key for researcher could be find out by expression performance. The aim of this study is to reveal the CGA as a candidate of antifibrotic & antioxidant in liver fibrosis that induced by CCL4. Methods: This is a pure experimental research with a simple experimental design or post-test only control group design. The total 29 mices of 2.5-month-old male Swiss mices with weigh 35-40 gram divided into 6 group: 3 groups of controls (injected by natrium chloride, CGA, and CCL4) and 3 groups of treated (injected by CGA doses 42 mg/kg, 63 mg/kg or 84 mg/kg). Liver organ was used to examine the expression of TLR-4 by rt-PCR. This research revealed that expression of TLR-4 lower than the CCL4 control group (respectively, p=0.042; p=0.005; p=0.006; and p=0.001). Higher dose of CGA showed greater ability as anti-fibrotic through inhibit the expression of TLR-4. Some research found the expression of TLR-4 has been decreased by treatment of Clorogenic Acid (CGA). Conclusion: To sum up, CGA has double roles to repair liver fibrotic tissues. The greater doses of CGA, the stronger inhibition of TLR-4 expression.

The Profile of Interferon-γ (IFN-γ) and Interleukin-10 (IL-10) in Pulmonary Tuberculosis Patients Widya Wasityastuti; Yanri W Subronto; Marsetyawan HNE Soesatyo
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4563

Introduction: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and becomes the main health problems in the world and in Indonesia, as well. The microorganism itself is an intracellular pathogen. The control of tuberculosis infection depends on cell-mediated immunity involving activated macrophages, T cells, and cytokines. The balance and dynamic changes between Th1 cytokine and Th2 cytokine refl ect the immune response of host and infl uence the clinical manifestation of the disease.Objectives: This research was designed to study the profi le and interaction of IFN-γ (Th1 cytokine) and IL-10 (Th2 cytokine) of pulmonary tuberculosis (PTB) patients in endemic area.Methods: Peripheral blood mononuclear cells of 23 pulmonary TB patients and 16 healthy persons was cultured and stimulated by phytohaemagglutinin (PHA) to investigate the ability to secrete IFN-γ and IL-10.Result: The result showed that there was a decreased of IFN-γ response to PHA in PTB patients, suggesting the defi ciency of general immune capacity in PTB. In contrast, IFN-γ secreted by specifi c antigen was higher in PTB patients which minimal lung lesion was higher than moderate-far advanced. It is related to IFN-γ roles as immunomodulator in cellular immunity and immunoprotectant through stimulated antimicrobial capacity in macrophage. In fact, IL-10 response to PHA and M.tuberculosis antigen in PTB patients was lower than that of in healthy persons; moderate-far advanced lung lesion was the lowest. It was probably refl ecting their poor general conditions. Paired distribution between IFN-γ and IL-10 pointed out the leaning of negative interaction. It refl ected the existence of counterpart/cross regulation between IFN-γ(Th1 cytokine) and IL-10 (Th2 cytokine).Conclusion: In conclusion that specifi c immune response of PTB is related to the degree of lung lesion. This study also provides the balance of Th1 cytokine and Th2 cytokine in relation to TB.Key words: tuberculosis, immune response, IFN-γ, IL-10, lung lesion

Tumor Necrosis Factor-Alpha (TNF-Alpha) And Intercellular Adhesion Molecule-1 (ICAM-1) Expression of Plasmodium Berghei Infected Swiss Mice Treated with Red Fruit (Pandanus Conoideus Lam) Ethanol Extract Demianus Tafor; Achmad Djunaidi; Widya Wasityastuti; Eti Nurwening Sholikhah
Tropical Medicine Journal Vol 3, No 2 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTIntroduction: Malaria infection could activate T cell helper 2 CD4+ which release cytokines IL-4, IL-5, IL-10 was a kind of immunosuppressant cytokines and prevented a cerebral tissue damage from the expression intercellular adhesion molecule-1 (ICAM-1) by TNF-α, ended with a cerebral malaria. The red fruit was proved as antioxidant but its effect to reduce TNF-α and expression of ICAM-1 in cerebral tissue endothelial cell remained unknown.Objectives: to know the effect of red fruit ethanol extract toward the change of TNF-α secretion number and ICAM-1 in Swiss male mice’s cerebral endothelial cell infected with P.berghei.Methods: Sixty Swiss male mice were divided in to 6 groups, group I was not given the red fruit (BM) ethanol extract and not infected P.berghei, Group II was given BM 260mg/kg BB but not infected P.berghei, group III, IV, V were given BM 130, 260, and 520 mg/kg respectively and infected with P.berghei. Red fruit extract or carrier substance was given for 4 weeks, P.berghei was given in the early of third week. TNF-α level check was done in third and ninth day after the injection, with TNF-α ELISA kit mouse. ICAM-1 cerebral endotel cell expression check was done in third and nine day with immunohistochemistry (IHC) coloring.Results: TNF-α level in third day after infected to the groups given red fruit extract (group III, IV, and V) showed no significant difference (p=0,839) compared to group which was not given red fruit but infected (group VI). TNF-α level in ninth day after infected to groups given red fruit ( group III,IV,V) lower (p<0,05) compared to groups which was not given red fruit but infected. ICAM-1 expression in third and ninth day after treatment to group given red fruit extract was lower (p<0,05) compared to groups given red fruit extract but infected. Conclusion: Red fruit ethanol extract ( P conoideus Lam) could decrease TNF-α level and ICAM-I expression in Swiss mice’ endothelial cerebral cells after infected by P.berghei.Key word: Pandanus conoideus Lam, TNF-α, ICAM-1 expression, Plasmodium berghei, Swiss mice

The Red Fruit (Pandanus Conoideus Lam) Ethanol Extract Decreased the Nitric Oxide (NO) Levels of Plasmodium Berghei Infected Swiss Mice Malaria Model but not the Interferon Gamma (IFN-γ) Mujur Mujur; Demianus Tafor; Widya Wasityastuti; Achmad Djunaidi; Eti Sholikhah Nurwening
Tropical Medicine Journal Vol 3, No 1 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.60593

ABSTRACTIntroduction: The immunity against malaria infection was very complex, involving humoral immunity and cellular immunity. One of the methods to improve body immune response was using antioxidant. Red fruit (Pandanus conoideus Lam) containing carotenoid and tocopherol that have antioxidants effects increase phagocytosis activity of macrophage and proliferation activity of lymphocyte as immune response againts parasite infection.Objectives: This study was intended to know the effect of red fruit (P. conoideus Lam) ethanol extract on IFN-γ and nitric oxide (NO) levels in Plasmodium berghei infected Swiss mice malaria model.Methods: This quasi experimental study used post test only control group design. Sixty eight-week aged male Swiss mice were devided into 6 groups. Group I was not given red fruit ethanol extracts neither infected by P.berghei, group II was given 260mg/kg/day of red fruit ethanol extracts, and it was not infected by P.berghei, groups III, IV and V were given red fruit ethanol extracts at 130, 260 and 520mg/kg/day respectively and infected by P.berghei. Group VI was not given the extract, however it was infected by P.berghei as control group. The red fruit ethanol extracts or the carrier was given for 28 days and the mice were infected by P.berghei in the beginning of week 3. On the day 3 and 9 after infection, 2 mL blood was taken from 3 mice of each group for serum isolation.Results: There was not any significant difference (p>0.05) mean of IFN-γ levels in all groups neither on day 3 nor day 9 after infection. The nitric oxide levels mean of mice group which have received red fruit extracts at 130 and 260mg/kg/day on the day 9 and in mice group which have received red fruit extracts 260mg/kg/day on day 3 were lower than they were in control group without any extract (group VI). However, the nitric oxide levels mean of mice group which have received 520mg/kg/day did not show any significant difference (p>0.05) compared with control group.Conclusion: The red fruit (P. conoideus Lam) ethanol extract did not show any significant effect on the IFN-γ levels, however at doses of 130 and 260mg/kg/day it decreased the nitric oxide level of P. berghei infected Swiss mice malaria model significantly. Keywords: Pandanus conoideus Lam, Interferon gamma (INF-γ), Nitric oxide (NO), Plasmodium berghei, and Malaria ModelKeywordsPandanus conoideus Lam, Interferon gamma (INF-γ), Nitric oxide (NO), Plasmodium berghei, and Malaria Model

BMPR2 Editing in Fibroblast NIH3T3 using CRISPR/Cas9 Affecting BMPR2 mRNA Expression and Proliferation Dwi Aris Agung Nugrahaningsih; Eko Purnomo; Widya Wasityastuti; Ronny Martien; Nur Arfian; Tety Hartatik
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1724

BACKGROUND: Bone Morphogenetic Protein Receptor II (BMPR2) deficiency is associated with the pathologic development of pulmonary vascular changes in Pulmonary Arterial Hypertension (PAH). Fibroblast is the most abundant cell in vascular. However, there is only a little information regarding the effect of BMPR2 deficiency in fibroblast. This study aims to understand the effect of BMPR2 deficiency in fibroblasts.METHODS: This study applied the CRISPR/Cas9 technique to edit BMPPR2 in NIH-3T3 cells. The transfection of CRISPR/Cas9 for BMPR2 editing into NIH-3T3 cells was done by using chitosan nanoparticles. The evaluation of BMPR2 and Transforming Growth Factor (TGF)-β mRNA expression was done using Quantitative real-time polymerase chain reaction. The assessment of edited NIH-3T3 cells proliferation was done using a scratch test assay.RESULTS: The BMPR2 mRNA expression of CRISPR/Cas9-edited group was lower than the untreated group. The proliferation of the CRISPR/Cas9-edited group was higher than the untreated group. The TGF-β mRNA expression of CRISPR/Cas9-edited and untreated groups was similar.CONCLUSION: BMPR2 deficiency in fibroblast increase the fibroblast ability to proliferate.KEYWORDS: BMPR2, PAH, fibroblast NIH-3T3, CRISPR/Cas9, proliferation

Pengaruh Edukasi AViRSex (Aid Video for Reproduction and Sexuality) terhadap Efikasi Diri Remaja Terkait Kesehatan Seksual dan Reproduksi di Kecamatan Ngemplak Etika Ratnasari; Sri Hartini; Widya Wasityastuti
Jurnal Keperawatan Klinis dan Komunitas (Clinical and Community Nursing Journal) Vol 5, No 3 (2021)
Publisher : PSIK FKKMK UGM

Background: Self-efficacy is the ability to organize or carry out an action in achieving goals. Adolescence is a vulnerable period, since in this phase adolescents are in the genital phase and their sexual urge reaches its peak. Sexual and reproductive health self-efficacy is a person's belief in making decisions regarding his or her sexuality. Education plays important role in adolescents regarding their decision regarding sexual and reproductive health. Therefore, it is necessary to conduct research on the effect of AViRSex Education toward adolescent’s sexual and reproduction health self-efficacy.Objective: To find out the effect of AViRSex (Aid Video for Reproduction and Sexuality) Education toward adolescent’s sexual and reproduction health self-efficacy.Methods: This was quantitative research with quasi-experiment design which was conducted in November-Desember 2019 and took 122 students from SMP N 1 Ngemplak and SMP N 2 Ngemplak Sleman as research subjects. This research used the adolescent sexual and reproduction health self-efficacy questionnaire with validity score 0,97 and reliability score 0,74. Data was analyzed using Paired t-test, Mann-Whitney, Pearson Product Moment, and Eta Test.Result: There was a significant change in the pretest and post-test score of the intervention group (p= 0,008) while there was not any significant change in the pretest and post-test score of the control group (p= 0,367). There was not any significant difference between the pretest and post-test score of the control and intervention groups (p= 0,067). There was no relationship between self-efficacy with age (r= -0,039) and sexual reproductive health experiences (Fcount = 2,062). There was a relationship between sex with self-efficacy (Fcount = 6,28).Conclusion: AViRSex Education could increase sexual and reproduction health self-efficacy score on 12-14 years old adolescent. ABSTRAKLatar belakang: Efikasi diri merupakan kemampuan untuk mengatur atau melaksanakan suatu tindakan dalam mencapai tujuan. Masa remaja merupakan masa rentan, dimana remaja berada fase genital dan dorongan seksual yang mencapai fase puncak. Efikasi diri kesehatan seksual dan reproduksi merupakan keyakinan seseorang dalam memutuskan terkait dengan seksualitasnya. Edukasi berperan penting dalam membuat keputusan terkait kesehatan seksual dan reproduksinya. Oleh karena itu, perlu dilakukan penelitian mengenai pengaruh Edukasi AViRSex terhadap efikasi diri terkait kesehatan seksual dan reproduksi pada remaja.Tujuan: Untuk mengetahui pengaruh Edukasi AVirSex (Aid Video for Reproduction and Sexuality) terhadap efikasi diri terkait kesehatan seksual dan reproduksi pada remaja.Metode: Penelitian ini merupakan penelitian kuantitatif dengan rancangan quasi-experimental. Penelitian dilaksanakan pada November – Desember 2019 dengan jumlah subjek sebanyak 122 siswa dari SMPN 1 dan SMPN 2 Ngemplak, Sleman. Instrumen yang digunakan adalah kuesioner efikasi diri kesehatan seksual dan reproduksi, dengan hasil validitas 0,97 dan reliabilitas 0,74. Analisis data yang digunakan yaitu Paired t-test, Mann-Whitney, Uji Pearson, dan Uji Eta.Hasil: Analisis data menunjukan adanya perubahan signifikan pada skor pre-test dan post-test kelompok intervensi (p= 0,008) dan tidak ada perubahan signifikan pada skor pre-test dan post-test kelompok kontrol (p= 0,367). Tidak ada perbedaan signifikan selisih skor efikasi diri pre-test dan post-test kelompok kontrol dan intervensi (p= 0,067). Tidak ada hubungan antara efikasi diri dengan usia (r= -0,039) dan pengalaman kesehatan seksual reproduksi (Fhitung= 2,062). Ada hubungan jenis kelamin dengan efikasi diri (Fhitung=6,28).Kesimpulan: AViRSex dapat meningkatkan skor efikasi diri kesehatan seksual dan reproduksi pada remaja usia 12 – 14 tahun.

Immunosenescence and the Susceptibility of the Elderly to Coronavirus Disease 2019 (COVID-19) Widya Wasityastuti; Andika Dhamarjati; Siswanto Siswanto
Jurnal Respirologi Indonesia Vol 40, No 3 (2020)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v40i3.115

A new disease caused by a newly-found coronavirus, known as Coronavirus Disease 2019 (COVID-19), has been declared as a pandemic by World Health Organization (WHO). This new disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The outbreak started in late December of 2019 and it did not take a long time until it spread to more than 200 countries in the world with over 4 million confirmed cases to date. The number keeps skyrocketing as day passes, followed by the increasing number of fatalities. Among all of the infected patients, elderly is one of the vulnerable populations. Studies reported that the risk of infection is doubled in older people. This infection-susceptible characteristic may be due to the weak immune system, therefore they lack the capabilities to fight the infection. The deterioration of immune system in elderly is known as immunosenescence. The aim of this literature review is to understand the effect of immunosenescence in the immunopathogenesis and susceptibility of elderly who are exposed to COVID-19 infection. (J Respir Indo. 2020; 40(3): 182-91)

Persepsi Mahasiswa terhadap Instruktur Sebaya pada Praktikum Pendengaran di Laboratorium Ilmu Faal Dakultas Kedokteran Universitas Gadjah Mada Gandes Endah Pakarti; Widya Wasityastuti; Yayi Suryo Prabandari
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 2, No 1 (2013): Maret
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Background:The traditional learning method applies at Physiology Laboratory of Faculty of Medicine at Universitas Gadjah Mada (UGM) is considered ineffective for the lecturers in managing the students. Moreover, the students perceive that their needs are not adequately accommodated by the lecturers. This downside can be resolved by an alternative learning method which provides conducive, active, creative, effective and enjoyable learning environment called peer assisted learning method. This study is aimed at discovering the students’ perceptions on peer assisted learning method and acquiring the students’ feedback and expectations on the effectiveness of peer-teaching method implementation.Method:The study used cross-sectional design of descriptive research.Results:From the questionnaire analysis with 150 respondents, 90 students of regular program and 60 students of international program, the result according to the frequencies of student perception were: 1) factor 1 were 45.8 % for very high category, 29.8 % for high category, 23.7 % for medium category, 0,8 % for very low category and 0 % for low category; 2) factor 2 were 45 % for high category,41.2 % for medium category,11.5 % for very high category,1.5 % for low category and 0.8 % for very low category; 3) factor 3 were 51.9 % for very high category, 31.3 % for high category,16.0 % for medium category,0.8% for very low category and 0% for low category; 4) factor 4 were 47.3 %for high category, 31.3 % for very high category,19.1 % for medium category,1.5 % for low category and 0.8% for very low category. There was no difference in perception between the students of regular and international program. Responses to open questions and FGD show a variety of students’ feedback and expectation on the peer assisted learning method.Conclusion:The students have shown positive perception on peer assisted learning as a learning method. The peer instructors required in Implementing this model are those students with good communication skill, confirmed academic qualification and capability in managing their fellow students’ learning.

Aplikasi Metode Sequential Three-Stage Integer Goal Programming untuk Penjadwalan Kuliah Nisrina Nafi’atul Huda; I Gusti Bagus Budi Dharma; Widya Wasityastuti
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 5, No 1 (2016): MARET
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Background: One of scheduling problems in educational sector currently was the course scheduling in college or well known as University Course Timetabling Problem (UCTP). The UCTP was comprehensive because of the policy difference among the university, faculties, and departments. The Faculty of Medicine Universitas Gadjah Mada, which organizes a block system has a different and more complex scheduling type than other faculties with non-block system. The porpose of this research was to build a mathematic model of block system UCTP in Faculty of Medicine Universitas Gadjah Mada to optimize the scheduling process time and results.Method: This research was conducted by dividing the scheduling problem into 4 sub – scheduling problems that are sequential, which are the scheduling of the 5 primary courses (lecture, panel discussion, practical sessions, tutorial, and skills laboratory), scheduling topics for each course, student’ group scheduling, and classroom scheduling. The problems are then modeled in the form of integer goal programming. Results: The block system scheduling model in Faculty of Medicine Universitas Gadjah Mada by considering topic and possibility of unavailability room using Sequential Three-Stage Integer Goal Programming has successfully developed and well verified.Conclusion: The model developed in this study is able to produce a schedule that meets all hard constraint in less than 24 hours. The result was more efficient compared to the previous trial-and-error method that consumed 30-45 days.

THE INVOLVEMENT OF LIPIDS IN IMMUNE SYSTEM REGULATION: A MINI-REVIEW Kurnia Putri Utami; Widya Wasityastuti; Marsetyawan HNE Soesatyo
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 1, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss1.art11

An immune system recognizes and responds to antigens entering the body. Maintaining these roles, components of the immune system need energy obtained from nutrients such as carbohydrates, proteins, and lipids. This study reviews and discusses roles of lipids, particularly fatty acids, in regulations of the immune system. This study was conducted by conducting a literature study on published research articles written in English. The articles were obtained from PubMed and Google Scholar by using search keywords: lipid, fatty acids, immune, regulation, inflammation, and response. Lipids are a group of biomolecule compounds composed of carbon, oxygen, and hydrogen, and they are classified into simple, compound and complex lipids. Fatty acids are compound lipids that act as a main fuel for metabolism, an essential component for all membranes, and a gene regulator. Fatty acids have a modulating effect on immune cells, such as: acting as a host defence, activating the immune system, interacting with nuclear transcription factors, playing roles in inflammatory responses, promoting apoptosis, as well as influencing lymphocyte proliferation, cytokine production, and Natural Killer (NK) cell activities. However, the modulation of the immune system by lipids is influenced by various factors such as concentration and types of fatty acids, types of immune cells, and species. This study is suggested to provide an overview of beneficial roles of lipids in maintaining immunity.

Co-Authors Achmad Djunaidi Achmad Djunaidi Andika Dhamarjati Anggorowati, Nungki Demianus Tafor Demianus Tafor Eko Purnomo Eti Nurwening Sholikhah Etika Ratnasari Gandes Endah Pakarti I Gusti Bagus Budi Dharma Kurnia Putri Utami Marsetyawan HNE Soesatyo Marsetyawan HNE Soesatyo Mujur Mujur Naibey, Rosdiana Nisrina Nafi’atul Huda Nugrahaningsih, Dwi Aris Agung Nur Arfian Nur Arfian, Nur Ronny Martien Siswanto Siswanto Sri Hartini Tety Hartatik Yanri W Subronto Yayi Suryo Prabandari

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