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All Journal Jurnal Ilmu Ternak Veteriner
I.W.T. Wibawan
Faculty of Veterinary Medicine, Bogor Agriculture Institute, Bogor, Indonesia
Veterinary

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The comparative study of antigenic protein characters of field isolates Brucella abortus cells with electrophoresis and immunoblotting Techniques Sudibyo, Agus; Pasaribu, Facriyan H; Wibawan, I.W.T.; Setiawan, Endhie
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 3 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.656 KB) | DOI: 10.14334/jitv.v1i3.32

Abstract

Live vaccine of Brucella abortus strain 19 has been used for prevention and eradication of brucellosis in cattle . The information of the use of this vaccine in Indonesia is still limited, while the effectivity, bacteriological and serological aspects of the vaccine are not much evaluated yet. The objective of this research is to study the differences of protein cell wall antigenicity profiles between B. abortus strain 19, strain 544 and field isolates. Protein cell wall was prepared by sonicafion of B. abortus S19, S544 and B. abortus field isolates biotype l, 2 and 3 . Antiserum against these B. abortus was prepared in cattle . Futhermore, the distribution of protein band was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), while protein antigenic profiles was examined by immunoblotting . The result showed that there was no significant different amongs protein cell wall of these B. abortus. From the antigenicity profiles exhibited that cattle vaccinated with B. abortus S19, no antibody was detected against protein less than 30 kDa. The other side natural infected or experimental infected cattle with B. abortus biotype 1 field isolate, antibody was detected until protein which has molecular weight about 15 kDa. Key words: Brucella abortus, antigenic protein, immunoblotting
Molecular characterization of Bovine herpesvirus type 1 Indonesian isolates Saepulloh, Muharam; Wibawan, I.W.T.; Sajuthi, D.; Setiyaningsih, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.846 KB) | DOI: 10.14334/jitv.v14i1.365

Abstract

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR
Co-Authors Agus Sudibyo D. Sajuthi D. Setiyaningsih Endhie Setiawan Facriyan H Pasaribu Muharam Saepulloh