Jurnal Ilmu Ternak dan Veteriner
Vol 14, No 1 (2009)

Molecular characterization of Bovine herpesvirus type 1 Indonesian isolates

Saepulloh, Muharam (Unknown)
Wibawan, I.W.T. (Unknown)
Sajuthi, D. (Unknown)
Setiyaningsih, D. (Unknown)



Article Info

Publish Date
07 May 2013

Abstract

Different subtypes of bovine herpesvirus 1 (BHV-1) have been associated with different clinical conditions of cattle. For that reason subtypes differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BHV infections. In search for a genomic region that would allow a clear distinction between BHV-1.1 and BHV-1.2 of glycoprotein D (gD) genes of 8 Indonesian isolates were amplified and sequenced. The amino acid sequence alignments revealed that the levels of genomic similarity ranging from 98.8 to 100% among BHV-1 Indonesian isolates and its results were also similar between BHV-1 Indonesia isolates and BHV-1.1 reference, and 98.4 to 98.8% between BHV-1 Indonesian isolates and BHV-1.2 reference. The isolates could be clearly separated into BHV-1.1 and BHV-1.2 after phylogenetic analysis. The results showed that the Indonesian isolates were characterized as BHV-1.1 as agent caused respiratory tract infections in cattle or infectious bovine rhinotracheitis (IBR) disease. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses. Key Words: BHV-1.1, BHV-1.2, Glycoprotein D, Phylogenetic Analysis, IBR

Copyrights © 2009






Journal Info

Abbrev

JITV

Publisher

Subject

Veterinary

Description

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic ...