Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Padjadjaran Journal of Dentistry

Effectiveness of mixed tea plant extract gel with chitosan on fibroblast cells after tooth extraction of Wistar rats Nyoman Ayu Anggayanti; Putu Lestari Sudirman; Ni Nyoman Rian Permata Sari; I Gusti Ayu Putu Diah Suryani
Padjadjaran Journal of Dentistry Vol 35, No 1 (2023): March 2023
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no1.36563

Abstract

ABSTRACTIntroduction: Tooth extraction can cause the damage of hard and soft tissue. When an injury occurs, fibroblast will respond in the wound healing process. Herbal medicines such as green tea and chitosan can be used in wound healing. The polyphenol content in green tea, namely EGCG, has an anti-inflammatory effect, increasing wound healing. In wound healing, chitosan promotes hemostasis and tissue recovery. Based on several previous studies, the use of 1.2% green tea extract was effective for improving wound healing in rat open wound models, chitosan 1% could trigger the proliferation of fibroblasts in the wound healing process after tooth extraction. The aim of this study is to analyze the effect of gel mixture of extract Camellia sinensis 1,2% with chitosan 1% on post-tooth extraction wound of Wistar rats. Methods: This was a randomized post-test only control group design using 40 Wistar rats which were randomly divided into 2 groups. Each group underwent intramuscular anesthesia on the rat's thigh and tooth extraction of mandibular left incisor. The treatment group was applied mixed gel of extract Camellia sinensis 1.2% and 1% chitosan as much as 0.01 ml and the control group was not given any treatment, after that decapitated on days 1, 3, 5, and 7. Result: The mean number of treated fibroblasts was higher than the control group on days 1, 3, 5, 7, respectively, 75.00; 176.00; 349.00 and 427.00 cells. The mean difference in the number of fibroblasts was significant with p-value 0.001 (p>0.05). Conclusions:  Mixed extract gel of Camellia sinensis and chitosan increased fibroblasts in wound healing process after tooth extraction of Wistar rats with the highest mean of fibroblast on the 7th day.Keywords: mixed tea leaves,chitosan, fibroblast,wound healing
Effectivity of 5% Temulawak Extract (Curcuma xanthorrhiza) on Post-Extraction Fibroblast Cells in Wistar Rats (Rattus norvegicus) Nyoman Ayu Anggayanti; Putu Lestari Sudirman; Made Indira Paramita
Padjadjaran Journal of Dentistry Vol 35, No 2 (2023): July 2023
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no2.47023

Abstract

ABSTRACTIntroduction: Fibroblast is a key element in the wound healing process. During the proliferation phase, fibroblast cells are important for producing collagen and elastic cells. Curcuma xanthorriza is a medicinal herb that contains active compounds such as curcumin and flavonoid, both of which have the ability to increase fibroblast migration and accelerate wound healing. This study aims to observe the effectiveness of 5% temulawak extract (Curcuma xanthorrhiza) on post-extraction fibroblast cells in Wistar rats (Rattus norvegicus). Methods: The experimental study was conducted on 30 rats, divided into a control group and a treatment group. The subjects received intramuscular anesthesia prior to extraction of mandibular incisor. The treatment group was injected with 5% curcuma extract into their wound socket, while the control group was given placebo gel injection. The mandibular samples were obtained and analyzed on the 3rd, 5th, and 7th day. The number of fibroblast cells was observed using a light microscope with three different fields of view at 400x magnification. Results: The mean number of fibroblasts in the experiment group was higher than that in the control group. On the 7th day, the treatment group showed 342.50 fibroblast cells, while the control group only showed 298.25 cells. The number of fibroblast cells in the 3rd day treatment group was comparable to that in the 5th day of control group. Conclusion: It can be concluded that 5% temulawak extract (Curcuma xanthorrhiza) has a significant effect increasing post-extraction fibroblast cells in Wistar rats (Rattus norvegicus).