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All Journal Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Zaky Nuron Rizky
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Mathematics

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Pengaruh 2,4-Dichlorophenoxyacetic Acid (2,4-D) DAN Benzyl Amino Purine (BAP) Terhadap Induksi Kalus Dari Berbagai Jenis Eksplan Tanaman Duku (Lansium domesticum Corr.) Mifta Ammaria Wulandari; Sherina Silva; Zaky Nuron Rizky; Jumiani Sarianti; Siti Zulaikha; Amin Nurokhman; Arif Yachya; Tutut Handayani; Syarifah; Dini Afriansyah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 15 No 01 (2022)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.15.01.5606.38-45

Abstract

Lansium domesticum Corr. is called Duku, an endemic Indonesian plant whose fruit has commercial value. The formation of the seeds of this plant takes a relatively long time (±15 years) for conventional propagation. Micropropagation with plant tissue culture techniques can be a solution. In the first phase of micropropagation, optimization of the type and concentration of growth regulators for callus induction is needed. This study aimed to determine the response of various types of duku explants (such as leaves, petioles, and mother petioles) to combinations of growth regulators (such as 0 ppm 2,4-D + 0 ppm BAP; 0.75 ppm 2,4-D + 0, 5 ppm BAP; 0.75 ppm 2,4-D + 0.75 ppm BAP; 0.75 ppm 2,4-D + 1 ppm BAP) in callus induction. The explants were cultured on Wood Plant Medium (WPM) with 30 g sucrose and 8 g agar for 28 days. The results showed that the concentration of 0.75 2,4-D + 0.75 BAP with leaf stalk mother explants was the best for callus formation. The characteristics of the callus formed were white-yellow with a compact texture. Keywords : Lansium domesticum Corr., Callus induction, Growth regulators, Types of explants.
PENGARUH 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) DAN BENZYL AMINO PURINE (BAP) TERHADAP INDUKSI TUNAS DARI EKSPLAN Folium DAN Petiolus communis TANAMAN DUKU (Lansium domesticum Corr.) Siti Zulaikha; Jumiani Sarianti; Miftah Amaria Wulandari; Sherina Silva; Zaky Nuron Rizky; Amin Nurokhman; Arif Yachya
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 15 No 02 (2022)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.15.02.6270.52-59

Abstract

Duku (Lansium domesticum Corr.) is an endemic plant of Indonesia that has a distinctive taste and is beneficial in the health sector. Besides that, the demand for duku seeds is increasing, so it is proven by the rapid propagation technique on a large scale. This can be achieved through tissue culture techniques. This study aimed to determine the effect of the growth regulators 2,4-D and BAP on Folium explants and Petiolus communis explants in inducing shoots. Leaf explants and leaf stalks were initiated using WPM (Woody Plant Medium) media with 4 different combinations of concentrations of growth regulators 2,4-D and BAP consisting of 0.0 ppm (2.4-D) + 0.0 ppm (BAP); 0.2 ppm (2.4-D) + 2.0 ppm (BAP); 0.2 ppm (2.4-D) + 2.5 ppm (BAP); 0.2 ppm (2.4-D) + 3.0 ppm (BAP) -D) + 2.0 ppm (BAP) on Petiolus communis explants is the most optimal combination of growth regulators, this is indicated by the growth response of the explants, namely the formation of brownish yellow callus on the explants.
Co-Authors Amin Nurokhman Arif Yachya Dini Afriansyah Jumiani Sarianti Mifta Ammaria Wulandari Miftah Amaria Wulandari Sherina Silva Siti Zulaikha Syarifah Tutut Handayani