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All Journal Indonesian Journal of Urology Padjadjaran Journal of Dentistry
Makmuri Yusuf
Department of Pathology, Faculty of Medicine/Padjadjaran University, Hasan Sadikin Hospital, Bandung.
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The influence of using platelet rich plasma for post-extraction wound healing process Oscar, Franky; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.555 KB) | DOI: 10.24198/pjd.vol22no1.14066

Abstract

Wound healing process characterized by specific processes of inflammation, proliferation, and maturation. It was coordinated by the regenerating and healing factors, including growth factor. Platelet-rich plasma was a blood component that separated from blood cell plasma, which contains a lot of growth factors for increased wound healing. The objective of this research was to observe the effect of the application of platelet-rich plasma for wound healing. The true experimental factorial design research was done to 24 rabbits (Oryctolagus cuniculus) which have been performed tooth extraction and application of platelet-rich plasma. These rabbits were terminated on 3rd, 7th, and 14th day, and the lower jaw region where the tooth had been extracted were cropped stained with hematoxylin-eosin for fibroblast and collagen histopathology examination. Based on ANOVA statistical analysis, the mean of fibroblast on the experimental group was 52.00+19.18 higher than the control group 24.83+10.46. Statistical tests showed a significant result of platelet-rich plasma application to fibroblast amount (p < 0.001). Collagen mean on the experimental group was 30.25+9.33 higher than the control group 13.75+6.96. It was significant statistically (p = 0.001). The conclusion of this research that wound healing process was influenced by the application of platelet-rich plasma.
Comparison of the effect of collagen (amino acid) and amnion (proteinase inhibitor) on the wound healing of soft tissues Adhiarto, Weko; Mangundjaja, Sunardhi; Yusuf, Makmuri; Pontjo, Bambang
Padjadjaran Journal of Dentistry Vol 22, No 1 (2010): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2000.307 KB) | DOI: 10.24198/pjd.vol22no1.14067

Abstract

The wound healing process of the soft tissue aims to accelerate the closing of the wound by recurring, restoring the function, and minimizing the scar tissue. This process has to occur in the surgery process to obtain better-wound healing. The aim of this study was to know the stimulation effect of the wound dressing to accelerate the wound healing process of the soft tissue using Collagen (amino acid) and Amnion (proteinase inhibitors). This true experimental study was done to 36 mice (Sprague Dawley) that were divided into three different groups, two groups were the treatment groups and one group was the control group. Each group was adapted in Pathology Laboratory of Faculty of Veterinary Medicine Bogor Agricultural University. All of the mice were injured on the skin by incising the right side of the back for 2 cm in length and 4 mm in depth and then cleaned with 0.9% NaCl solution. To the first group of treatment, the wound was applicated by Amnion, and the second group was applicated by Collagen. Each 4 mice of all group were a termination by ether and encryption on the wounded tissue was conducted on day 3rd, 7th, and 14th. Assessment for a number of fibroblast and collagen synthesis on the wound of back tissue was conducted with the histological examination by painted using hematoxylin-eosin. The results concluded that Collagen application was influencing the mean of fibroblast higher than Amnion, and the lowest was controlled with statistically significant. The mean of collagen used Collagen lower than Amnion, and the lowest was control with statistically significant. It could be concluded that collagen application was preeminent in increasing the productivity of the fibroblast, as well as in developing forms of the collagen synthesis compared with Amnion and control.
COMPARISON BETWEEN GLYCOGEN LEVEL OF KIDNEY TUBULAR CELLS IN DIABETIC KIDNEY STONE PATIENTS AND NON DIABETIC KIDNEY STONE PATIENTS Ramadhan, Ken; Sugandi, Suwandi; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati; Safriadi, Ferry; Siregar, Safendra; Adriansjah, Ricky; Adi, Kuncoro; Sihombing, Aaron Tigor; Yusuf, Makmuri
Indonesian Journal of Urology Vol 18 No 1 (2011)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v18i1.76

Abstract

Objective: To compare glycogen level of kidney tubular cells in diabetic kidney stone patients with nondiabetic kidney stone patients. Material & method: We reviewed kidney stone patients who underwent nephrectomy in Hasan Sadikin Hospital from February 2008 to January 2009. Age, gender, type of DM, and urine pH were recorded. Glycogen level of kidney tubular cells were evaluated histochemically using HE, PAS, and PAS diastase. The results were categorized into 3 grades, based on the staining appearance compared to liver cells as controls. The results of diabetic group were compared to non diabetic patients. Results: There were 30 patients eligible for this study, 15 in diabetic group and 15 non diabetic patients. In the diabetic group, there were 10 patients with urine pH < 6 and 5 patients with a pH of 6,5. In the non diabetic group all urine pH were > 6,5. Examination revealed that glycogen level of kidney tubular cells in diabetic group was higher than in non diabetic group. Conclusion: Glycogen level of kidney tubular cells in diabetic kidney stone patients was higher than in non diabetic kidney stone patients. Keywords: Tubular cell, kidney stone, diabetes mellitus.
Co-Authors Aaron Tigor Sihombing Bambang Pontjo Priosoeryanto Bambang S Noegroho Ferry Safriadi Franky Oscar, Franky Ken Ramadhan Kuncoro Adi Ricky Adriansjah Safendra Siregar Sunardhi Mangundjaja, Sunardhi Suwandi Sugandi Tjahjodjati Weko Adhiarto, Weko