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TINGKAT KEBERHASILAN INSEMINASI BUATAN PADA BABI MENGGUNAKAN SEMEN YANG DIBERI EKSTRAK MESOCARP Borassus ?abellifer Linn YANG DI PRESERVASI SELAMA 4 HARI PADA SUHU13°C Hermilinda Parera; Bernadus Ndoen; Yonas Lino; Nathan Adoe
Partner Vol 23, No 1 (2018): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i1.294

This study aims to determine the succesfull of artificial insemination (AI) in sows using semen added with 0,01% mesocarp extract of Borassus ?abellifer Linn as an antioxidant source and preserved at 13 °C for four days. This study used 16 Duroc-Landrace crossbred sows with ages of 1-3 years that had given birth for at least 1 time. The sows was divided into two groups: group I (K1) using semen with Beltsville Thawing Solution (BTS) diluent preserved at 13 °C for 3 days, and Group II (K2) usingsemen with BTS diluent given 0.01% extract mesocarp B. flabellifer Linn and preserved at 13 ° C for four days. Evaluation of the success rate of artificial insemination were described by the number of non retrun rate (NRR), service per conception (S/C) and conception rate (CR), which continued analyzed using t-test. The results showed that the NRR value of the K1 and K2 were not significantly different (P> 0.05) between the two groups with values of 80% and 77,7%, respectively. The S/C value of group K1 was 1.25 was not significantly different (P> 0.05) from the K2 with the value of 1.125. Whereas, the value of CR of the K1 is 75% showed no significant difference with the K2 87.5% (P> 0.05). It can be concluded that the use of semen given BTS diluent and 0.01% mesocarp extract B.flabellifer Linn and preserved at 13 ° C for up to 4 days can be used in artificial insemination, because it has the same capabilities with semen that given BTS diluent and preserved at 13 ° C for 3 days.

PENINGKATAN MANAJEMEN KESEHATAN BABI DAN PERTANIAN TERPADU DI KELOMPOK MAWAR DAN KELOMPOK LOROSAE Hermilinda Parera; Jois M. Jacob
Jurnal Pengabdian Masyarakat Peternakan Vol 1, No 1 (2016): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Kelompok Mawar dan Lorosae adalah dua kelompok tani ternak yang berada di RT III desa Manusak Kabupaten Kupang yang sebagian besar anggotanya adalah warga eks Timor-Timur. Anggota kelompok ini bermata pencaharian bertani dan beternak, dengan komoditi utama sayuran dan usaha beternak babi. Model usaha peternakan masih bersifat tradisonal sehingga peternakan masih dianggap sebagai usaha sampingan. Tujuan yang akan dicapai dari rencana implementasi IbM ini adalah: a) menjalin kerjasama dengan kelompok Lorosae dan Mawar; b) meningkatkan produktivitas ternak babi melalui perbaikan manajemen pemeliharaan dan kesehatan, seperti pembuatan kandang babi yang memenuhi standar, menerapkan pelayanan kesehatan melalui vaksinasi, pemberian obat cacing, vitamin dan pengobatan penyakit; c) pemeberdayaan kelompok dalam memanfaatkan limbah pertanian untuk usaha beternak ikan air tawar melalui pembuatan kolam lele dan pembuatan pakan lele dengan teknologi ramah lingkungan; e) meningkatkan nilai ekonomis limbah peternakan melalui pembuatan bokasi; f) perbaikan manajemen usaha beternak babi dan lele melalui model pertanian terpadu. Perbaikan manajemen pemeliharaan dan kesehatan ternak babi dan penerapan teknologi yang ramah lingkungan diharapkan dapat meningkatkan kesejahteraan melalui peningkatan pendapatan keluarga.Â Kata kunci: kelompok Lorosae dan Mawar, pertanian terpadu, ikan lele dan kesehatan ternak babi.

EFEKTIVITAS PENAMBAHAN EKSTRAK MESOCARP Borassus flabellifer PADA PENGENCER BELTSVILLE THAWING SOLUTION TERHADAP VIABILITAS SPERMATOZOA ASAL KAUDA EPIDIDIMIS BABI Hermilinda Parera; Bernadus Ndoen; Victor Lenda; Muhammad Mirandy Pratama Sirat
JURNAL ILMIAH PETERNAKAN TERPADU Vol 7, No 1 (2019)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

This study aimed to determine the effectiveness of adding various concentrations of palm fruit mesocarp (Borassus flabellifer) extract to Beltsville Thawing Solution (BTS) diluents on the viability of swines cauda epididymis spermatozoa at 13°C for 4 days. During the storage period, the metabolic activity of spermatozoa will produce free radicals which can reduce spematozoa viability. Borassus flabellifer mesocarp extract contains antioxidants that can counteract free radicals. Testicular and epididymal samples from the 3-4 years old Duroc Landrace Swine Crossbreed. Swine cauda epididymis were obtained from the Oeba Abattoir, Kupang. Cauda epididymis spermatozoa were collected using a combination of incision, rinsing and suppression methods then collected and diluted using BTS and divided into four groups to be given various concentrations of Borassus flabellifer mesocarp extract (K0: without extract; K1: 0.01%; K2: 0.03%; and K3: 0.05 %) and preserved at 13°C for 4 days. Evaluation of spermatozoa viability was carried out microscopically. The results of cauda epididymis sperm viability were K0 = 43.57%; K1 = 54.81%; K2 = 50.52%; and K3 = 49.95%. Analysis of variance showed that the addition Borassus flabellifer mesocarp extract gave a significant difference (P> 0.05) to spermatozoa viability. The Duncan region test showed a significant difference (P <0.05) between treatment K1 with treatments K0, K2 and K3 on the viability of spermatozoa. The conclusions of this study was the addition of Borassus flabellifer mesocarp extract at 0.01% concentration was the most appropriate dose to maintain the viability of swine cauda epididymis spermatozoa. Keywords: Borassus flabellifer, Cauda Epididymal, Mesocarp Extract, Spermatozoa, Swine.

EFEKTIVITAS PENAMBAHAN EKSTRAK MESOCARP Borassus flabellifer PADA PENGENCER BELTSVILLE THAWING SOLUTION TERHADAP VIABILITAS SPERMATOZOA ASAL KAUDA EPIDIDIMIS BABI Hermilinda Parera; Bernadus Ndoen; Victor Lenda; Muhammad Mirandy Pratama Sirat
JURNAL ILMIAH PETERNAKAN TERPADU Vol 7, No 1 (2019)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

This study aimed to determine the effectiveness of adding various concentrations of palm fruit mesocarp (Borassus flabellifer) extract to Beltsville Thawing Solution (BTS) diluents on the viability of swines cauda epididymis spermatozoa at 13°C for 4 days. During the storage period, the metabolic activity of spermatozoa will produce free radicals which can reduce spematozoa viability. Borassus flabellifer mesocarp extract contains antioxidants that can counteract free radicals. Testicular and epididymal samples from the 3-4 years old Duroc Landrace Swine Crossbreed. Swine cauda epididymis were obtained from the Oeba Abattoir, Kupang. Cauda epididymis spermatozoa were collected using a combination of incision, rinsing and suppression methods then collected and diluted using BTS and divided into four groups to be given various concentrations of Borassus flabellifer mesocarp extract (K0: without extract; K1: 0.01%; K2: 0.03%; and K3: 0.05 %) and preserved at 13°C for 4 days. Evaluation of spermatozoa viability was carried out microscopically. The results of cauda epididymis sperm viability were K0 = 43.57%; K1 = 54.81%; K2 = 50.52%; and K3 = 49.95%. Analysis of variance showed that the addition Borassus flabellifer mesocarp extract gave a significant difference (P> 0.05) to spermatozoa viability. The Duncan region test showed a significant difference (P <0.05) between treatment K1 with treatments K0, K2 and K3 on the viability of spermatozoa. The conclusions of this study was the addition of Borassus flabellifer mesocarp extract at 0.01% concentration was the most appropriate dose to maintain the viability of swine cauda epididymis spermatozoa. Keywords: Borassus flabellifer, Cauda Epididymal, Mesocarp Extract, Spermatozoa, Swine.

GAMBARAN HISTOPATOLOGI BEBERAPA TIPE BRONCHOPNEUMONIA PADA PULMO BABI HASIL PEMOTONGAN DI RPH OEBA Hermilinda Parera; Aven B Oematan; Victor Lenda
JURNAL KAJIAN VETERINER Vol 5 No 2 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i2.951

The aim of this study was to determine the histopathologic changes of bronchopneumonia type in pigs of slaughtered pig from Oeba slaughterhouse associated with P. multocida infection. Samples of pulmo were found from pigs showing respiratoric symptoms, characterized as dyspnoea and presence of nasal exudates that are serous to mucopurulent, obtained from slaughtered pigs in RPH Oeba Kota Kupang. Samples showed pathognomonic lesions of gray hepatized than processed for histopathological studies. The result showed that varied macroscopic changes noted in pulmo samples from suspected infected pigs showed various types of lesions. Lessions observed then classified into two main lessions were acute and chronic. Acute lesions were characterized by edema and hyperemia which then confirmed with congestion and multifocal haemorrhages. Serous to seromucous exudates were observed intrabronchial and bronchioles. In chronic cases, the lesions showed cranioventral consolidation and gray hepatized with demarcated area beetwen consolidated and normal pulmonary area. Histologically, the types of bronchopneumonia categorized into bronchopneumonia suppurativa characterized by coagulative necrosis surrounded by cell infiltration and accumulation of fibrin intraalveolar. The fibrous connective tissue were also observed in the interstitaial space of alveolar around the necrotic area. Other samples showed non-suppurativa fibrinous bronchopneumonia with alveolar exudates dominated by mononuclear cells and accumulation of fibrin intra alveolar. Chronic bronchiolitis also seen with a mixture of mononuclear cells, neutrophils and macrophages that infiltrated into the lumen of the alveoli. A high number of goblet cells were observed in the bronchiolus epithelium, with the thickening of the fibrous tissue around the bronchioles. It can be concluded that there are two major types of lesions which are acute and chronic, with three types of bronchopnemonia namely bronchopneumonia suppurativa, non supuratival bronchopneumonia and chronic bronchiolitis.

Tingkat Fertilisasi Oosit Sapi Silangan Simmental Peranakan Ongole dan Limousin Peranakan Ongole Secara In Vitro Hermilinda Parera
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.985

Crossbreeding program by artificial insemination in Indonesian have use from Simmental and Limousin to local cows of Ongole Grade. Offspring from this crossbred called SimPO and LimPO which has advantages such as a large birth weight and rapid growth. The disadvantages of SimPO and LimPO cows are decreased of reproduction performance of Fenotipe 2 (F2) such as pregnancy rate being lower. Pregnancy stage will occur when oocyte had fertilized and had reached to embryos cleavage stage.The aims of this research was to determined in vitro fertilization rate of SimPO and LimPO oocyte. Ovaries from local abbotoir grouped into PO (control), SimPO and LimPO. Cumulusoocyte complexes quality A and B were used for this research. Oocytes were fertilized using frozen semen of Simmental with concentration 5 x 106 cells / ml in Brackett oliphant (BO) medium. In vitro fertilization rate to observed polymorphonuclear (PMN) formation 10 hours after insemination using 1% aceto orcein staining to fertilized oocytes. Fertilized oocytes were washed were transferred into culture and incubated at 38,5 ° C, with 5% CO2 and 95% humidity. These results indicate that in vitro fertilization rate of oocytes did not any significant differences between groups

Negative Energy Balance dan Days Open Pada Berbagai Tingkat Paritas Partus Sapi Fries Holland Bambang Hadisutanto; Hermilinda Parera
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.996

Negative Energy Balance is a transition period that is transitional between 3 weeks before parturition until 3 weeks after parturition. Days open is the interval between calving parturition with marriage that produced approximately 85 days of gestation. Parity is a period in the reproductive cycle of cattle with an indication of the number of livestock parent parturition. The purpose of research is to find out the negative energy balance and open days at various parity cows parturition Fries Holland.This study design was used to observe the one-way classification parent dairy cows after parturition I, II and III respectively as many as 30 individuals. Data observation glucose levels and days open were analyzed by the General Linear Model (GLM) and followed by DMRT to determine the significance level of blood glucose levels and days open between parity parturition.The results showed that the blood glucose levels of 30 breeding dairy cows at parturition turns holding each parity parity I showed significant differences (51.57 ± 5.56 mg / dL) compared to the parent parity II (45.57 ± 8.01 mg / dL) and parent parity III (46.7 ± 8.62 mg / dL). While holding Open Days parity I (106.07 ± 38.11 days) showed a longer time than the days of open parent parity II (86.23 ± 29.00 days) and III (89.23 ± 38.22 days).The conclusion of this study is negative energy balance which were carried in the blood glucose levels are still within the normal range is 33-55 mg / dL. While holding open days longer than the first parity with the parent parity II and III.

Pengaruh Ukuran Ovarium Dan Diameter Oosit Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor Yang Dikoleksi Secara In Vitro Hermilinda Parera
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.998

One of the reproductive technology in cattle which growing rapidly is in vitro fertilization techniques for in vitro embryo production. Environmental factors that influence the in vitro embryo development is the diameter of oocytes and ovarian size. Currently, the amount of research on Bali-Timor cattle is still lacking, especially in reproduction tecnology. Kune and Solihat (2007) states that the Bali-Timor cattle have a high fertility rate up to 60-70%. Application of in vitro reproduction technology can improve the genetic quality of livestock by using gametes cells collected from animals. The purpose of this study was to determine the effect of the size of the ovaries and oocytes diameter to the quality of the oocyte morphology of Bali-Timor cattle that were collected by in vitro technique. Oocytes samples of Bali-Timor cattle derived from ovarian which obtained from the slaughter house. Furthermore, the ovaries were grouped based on the size of the ovary, i.e group I (≤1,5 cm); group II (1.6 to 2 cm) and group III (≥2,1cm). Oocytes produced from the ovarian then grouped based on the diameter of the oocytes, i.e group I (110-150μm); group II (151-200μm); group III (201-250μm); group IV (251-300μm); Group V (301-350μm) and group VI (≥ 351μm). The results showed that the quality of the oocyte morphology did not differend significantly (P> 0.05) on ovarian size. Oocyte quality category A (29.78%) and category B (42.55%) were obtained from group III ovarian higher than group II (25% category A, category B 36%) and group I (category A 19.23 %, category B 26.92%), while the diameter of the oocytes had no effect on the percentage of morphological quality of oocytes produced in vitro.

Identifikasi Molekuler Pasteurella multocida Penyebab Pasteurellosis Pada Babi Di Yogyakarta Victor Lenda; Hermilinda Parera
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1027

The purpose of this study was to determine the genetic variation of P.multocida in Yogyakarta with other overseas P. multocida strains. Samples were obtained from necropted intensive pig farming with clinically pneumonia symptoms, specific pulmonary lessions and then followed by histopathological examination and b iochemical characterization of the isolates. Then followed by histopathological examination and biochemical characterization of the isolates. Moleculartest performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5' GGA GTG AAC TGC AGC TAA TAC C 3', and reverse primer 5' GTA GGT AAG CTT CGC GTT GTT G 3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing r esults were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed gen etic distance based on 765 nucleotides of 16S rRNA gene of P. multocida isolates from lungs of bronchopneumonia of swine in Yogyakarta at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta a nd other isolates from USA, Germany, China, Europe, and Hungaria.

Pengaruh Corpus Luteum Dan Folikel Dominan Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor Hermilinda Parera; Victor Lenda
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1031

Improvement of cattle production can be obtained by the application of feed and breeding technology. One of the breeding technologies that are rapidly growing is in vitro fertilization, which is part of In vitro embryo production The source of oocytes influence the success of in vitro embryo production The quality of the oocyte were determined by Ovarian follicular environment and followed the follicular growth that were characterized by the presence of a dominant follicle (DF) and corpus lutem (CL). The purpose of this study was to determine the influe nced of dominant follicles and corpus luteum on oocyte morphology ofBali Timor cattle, that were collected by aspiration method. Oocytes were obtained postmortem from the ovaries of Bali Timor cows slaughtered at an Oeba slaughterhouse Ovarian then class ified into 3 groups : I ) ovarian with CL without DF; (II) ovarian with DF, without CL; and (III) ovarian without both DF and CL. The results showed that the number of oocytes with good quality from group III (12.5%) was higher than group I (9.83%), but no significant differently (P> 0.05). The number of oocytes from group II that showed several layers of cells and has a cytoplasm (good quality) was very low.

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