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Histopathological Features of Duodenal Coccidiosis in the Probiotic Broiler Chickens Aven B Oematan; Damai Kusumaningrum
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This aim of research was to know histopathology lesions of broiler duodenum in chicken coccidiosis with probiotic administrasion. Sixty six DOC of broiler, strain Arbor acress, male sex were divided into 5 groups of twelve each. Chickens in Groups I, II, III, IV and V were given standard food, standard food+probiotic, standard food+1000 oocysts, standard food+probiotic+1000 oocysts and standard food+probiotic+ 3000 oocysts,respectively. Probiotic was given starting at the age of 8 days by sprayed in food with the dose 2 ml/ 250 ml water/ 1 kg food. At the age of 38 days, chickens were infected orally by given 1000 oocysts for group III and IV, and 3000 oocysts for group V. Group I and II were euthanazed at the age of 39 days, group IIIA, IVA, VA at 5 days postinfection, group IIIB, IVB, VB at 6 days post infection, and group IIIC, IVC, VC at 7 days post infection. Histopathologic lesions of the duodenums were analyzed with qualitative analyze. Results showed that infection without probiotic area were found histopathologic changes of duodenum in the form of congestion, hemorrhages, schizonts and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Treatment infection with probiotic were found histopathologic changes in the duodenum, such as congestion,hemorrhages, necrotic epithelial cells, and also schizonts, microgametes and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Probiotic in the present study did not have any effect for histopathologic changes in the duodenum in the broiler chickens infected coccidiosis.

Gastrointestinal Endoparasites Diversity of Macaca fascicularis in Goa Monyet Tenau Garden, Kupang I Gusti Komang Oka Wirawan; Damai Kusumaningrum; Aven Bernard Oematan
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Goa Monyet Tenau Garden is one of the tourist parks located in Kupang East Nusa Tenggara Province. The population of long-tailed macaque (Macaca fascicularis) is about 142 in the region, while outside the region is 162. This study was aimed to determine the types of gastrointestinal endoparasites that infect monkeys which live inside and outside the region, and to study if there is a relationship between different regions with percentageof gastrointestinal endoparasites that infect monkeys as well. Specific target was to obtain data on the diversity of endoparasites in the tourist park. Macaca fascicularis fecal sampling was done randomly from inside and outside the region. Thirty fecal samples were taken which composed of 15 samples from the region and 15 samples from outside the region. The weight of each fecal sample is ± 10 gr. Each fecal sample was inserted into the pot(capacity ± 25 ml) and labeled by the place, date and month of collection. The samples were given 10% formalin as a preservative. Examinations were conducted at the Animal Health Laboratory Politani Kupang, using 2 methods : Centrifuge and Sedimentation method. Diversity and percentage of worm infection in monkeys between different regions were analyzed descriptively. The results showed that the types of endoparasitesinfecting monkeys inside and outside of the region were the same (Eimeria sp., Ancylostoma sp., and Strongyloides sp.). This was caused by the interaction of the use of the same region. Percentage average of endoparasitic infection that infects monkeys in the region was higher than that of outside the region (In the region: Eimeria sp./ 86.7%, Ancylostoma sp./ 73.3% and Strongyloides sp./66.7%, outside the region: Eimeriasp./ 80%, Ancylostoma sp./60%, Strongyloides sp./46.7%. The difference percentages of infection were influenced by several factors, such as vegetation, water sources, sanitation, season, temperature and population.

TOTAL LEUKOSIT DAN DIFERENSIAL LEUKOSIT DARAH AYAM KAMPUNG YANG TERPAPAR ASCARIDIA GALLI SECARA ALAMI Devi Yasintha Moenek; Aven B. Oematan; Novianti N. Toelle
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.365

This study aims to determine the total number of leukocytes and differential leukocytes of native chicken blood exposed to Ascaridia galli naturally. Sampling of free-range chicken blood was carried out in Amarasi Subdistrict of Kupang Regency while blood tests were carried out at the Veterinary UPT Laboratory of the Animal Husbandry Department of NTT Province. The variables observed were blood figures, especially the total number of leukocytes, lymphocytes, monocytes, neutrophils, basophils, and eucinophils. The blood picture data obtained will be adjusted to the normal blood picture then all data obtained will be processed descriptively. From examination of stool samples found Ascaridia galli worm eggs for all stool samples and blood tests showed that the total number of leukocytes was 112.5 X 10 ^ 3 / µL, lymphocytes 86 X 10 ^ 3 / µL, monocytes 4.2 X 10 ^ 3 / µL, neutrophils 5.2 X 10 ^ 3 / µL, basophils 0, and eusinofil 4.6 X 10 ^ 3 / µL. Keywords: Ascaridia galli, Ayam Kampung, Total Blood leukocytes, Differential leukocytes

IDENTIFIKASI MIKROBIOLOGI (STAPHYLOCOCCUS DAN COLIFORM) PADA SUSU DAN DAGING SERTA OLAHANNYA DI KOTA JOGJAKARTA Ni Sri Yuliani; Aven B. Oematan
Partner Vol 20, No 1 (2013): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v20i1.7

Microbiology Identification (Staphylococcus and Coliform) of Milk, Meat and Their Processed Products in Jogjakarta City. Food is a perishable foods by microorganisms that can be harmful to consumers' health. Staphylococci and coliform commonly found in foods. The objective of this study was to know the type of contaminants in samples of meat and milk and processed products and growth in culture media. Materials examined were dairy and meat and dairy that come from the city of Jogjakarta. This study was conducted in August 2010 at the Laboratory of Veterinary Public Health Faculty, Gadjah Mada University. The method used in the microbiological examination is the method of casting and counting the cup.The results obtained from both methods of inspection showed there Staphylococcus and coliform contaminants in the material examined. The number of bacteria Staphylococcus sp on Vogel-Johnson media showed a high coliform in fresh milk while Violet Red Bile media found in raw milk and raw chicken meat. The results obtained in excess of standards established SNI No. 01-7388-2009. Obtained from the examination can be concluded that the number of bacteria exceeds SNI standards are set. Reklama: Bendradarbiaujame su stambiausiai Lietuvos ir užsienio automobiliu daliu tiekejais bei gamintojai todel auto detales galime pasiulyti geromis kainomis UAB ŠIAULIU AUTODOTA Keywords: Staphylococcus, Coliform, Milk, Meat

PEMANFAATAN EUPATORIUM ODORATUM SEBAGAI INSEKTISIDA ALAMI PENGENDALI LALAT Ni Sri Yuliani; Aven B. Oematan; Viktor Lenda; Gerson Y.I. Sakan
Partner Vol 23, No 1 (2018): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i1.290

Flies are vectors of various diseases in livestock. Eupatorium odoratum plants are bush plants that thrive and have antibacterial, antifungal and insecticidal activity. The purpose of this research is to know the effectiveness of Eupatorium odoratum extract as a natural insecticide, easy to get and safe. Flies were taken from the farmyard location, then grouped into 4 of 40 each per group. Flies were treated with Eupatorium odoratum extract at concentrations of 25%, 50% and 75%, while the controls were not treated. Observation of extract activity on flies mortality was taken within 3 hours, 6 hours to 12 hours after treatment. The data were analyzed descriptively and showed that the treatment result was not effective. Flies adapted in experimental cages do not get food (blood sucking activity at the host), so flies will die or flies can not survive outside the host's body.

KERAGAMAN DAN AKTIFITAS LALAT PENGGANGGU DI PETERNAKAN SAPI SEMI EKSTENSIF Aven Bernard Oematan; Devi Y J A Moenek
Partner Vol 23, No 2 (2018): Edisi November
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i2.315

Flies are an important vector in directing disease to livestock. It can cause economic losses for farmers such as decreasing the selling price of livestock products, especially meat and milk while losses to livestock are blood loss and weight loss. The aim of this study was to determine thediversity of species, patterns of activity of flies and their relationship to temperature, season, and sex. The research was carried out on semi-intensive cattle farms in the Oli'o dalam kom pasture and Kebun Nitas in Kupang Timur District, Kupang Regency, East Nusa Tenggara. Flies samples were collected using the New Zealand 1 trap. Traps are installed randomly directly at the grazing location, while in cages, traps are placed at a distance of 3-5 meters from the cage guardrail. NZ1 traps are installed from 08:00 until 16:00, then the collection of fly samples is carried out at intervals of two hours from the time of installation, namely 10: 00, 12: 00, 14: 00 and 16: 00. Flies were collected and euthanized with 70% alcohol, then identified by species with identification keys (Dodge 1967) and(Masmeathathip, 2013). Then the data were analyzed descriptively and SEM, from the results of the research obtained by Musca domestika flies, Musca stabulans, Fannia canicularis, haematobia irritans and Tabanus striatus

UJI KARAKTERISTIK SEDIAAN SALEP EKSTRAK ETANOL BUAH MAKASAR (Brucea javanica [L.] Merr) SEBAGAI KANDIDAT SALEP UNTUK LUKA INCISI DAN LUKA DIABETES Jois Moriani Jacob; Aven B. Oematan; Yorida Febry Maakh
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.6614

Makasar fruit (Brucea javanica [L]. Merr) has been widely used as herbal medicine to treat various types of diseases through simple processing. However, the use of Makasar fruit drugs with topical application as an ointment to treat incision wounds or diabetic wounds is extremely rare. The purpose of this research is to produce ointment and to assess the physical quality of the ointment made from the ethanolic extract of the Makassar fruit. The ointment used in this study was made with ethanol extract of Makassar fruit, alpha tocopherol, propyl-paraben, and Vaseline album. The data were observed descriptively and using a qualitative method. This study used two ointment concentrations, 15% and 20%, which were tested three times for each physical evaluation (organoleptic test, homogeneity test, dispersibility test, and pH test). The results showed that the ethanol extract of Makassar fruit could be formulated as an ointment, and the physical evaluation of the ointment discovered that the ointment met the organoleptic standards and the homogeneity standards. Furthermore, the pH test revealed that the ointment was safe to use; however, only 1 sample (repeat 1 concentration of 20%) confirmed that it did not meet the pH requirements because it was lower than the standard (acid pH). Furthermore, the dispersion tests indicated that the ointment did not meet the ointments dispersion standard. Conclusion: because the physical examination showed that the ointment met the standard values, the ointment can be made from the ethanol extract of Makassar fruit. However, because the ointment's spreadability is below the standard value, it must be taken into account.

GAMBARAN HISTOPATOLOGI BEBERAPA TIPE BRONCHOPNEUMONIA PADA PULMO BABI HASIL PEMOTONGAN DI RPH OEBA Hermilinda Parera; Aven B Oematan; Victor Lenda
JURNAL KAJIAN VETERINER Vol 5 No 2 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i2.951

The aim of this study was to determine the histopathologic changes of bronchopneumonia type in pigs of slaughtered pig from Oeba slaughterhouse associated with P. multocida infection. Samples of pulmo were found from pigs showing respiratoric symptoms, characterized as dyspnoea and presence of nasal exudates that are serous to mucopurulent, obtained from slaughtered pigs in RPH Oeba Kota Kupang. Samples showed pathognomonic lesions of gray hepatized than processed for histopathological studies. The result showed that varied macroscopic changes noted in pulmo samples from suspected infected pigs showed various types of lesions. Lessions observed then classified into two main lessions were acute and chronic. Acute lesions were characterized by edema and hyperemia which then confirmed with congestion and multifocal haemorrhages. Serous to seromucous exudates were observed intrabronchial and bronchioles. In chronic cases, the lesions showed cranioventral consolidation and gray hepatized with demarcated area beetwen consolidated and normal pulmonary area. Histologically, the types of bronchopneumonia categorized into bronchopneumonia suppurativa characterized by coagulative necrosis surrounded by cell infiltration and accumulation of fibrin intraalveolar. The fibrous connective tissue were also observed in the interstitaial space of alveolar around the necrotic area. Other samples showed non-suppurativa fibrinous bronchopneumonia with alveolar exudates dominated by mononuclear cells and accumulation of fibrin intra alveolar. Chronic bronchiolitis also seen with a mixture of mononuclear cells, neutrophils and macrophages that infiltrated into the lumen of the alveoli. A high number of goblet cells were observed in the bronchiolus epithelium, with the thickening of the fibrous tissue around the bronchioles. It can be concluded that there are two major types of lesions which are acute and chronic, with three types of bronchopnemonia namely bronchopneumonia suppurativa, non supuratival bronchopneumonia and chronic bronchiolitis.

ENDOPARASIT PADA USUS AYAM KAMPUNG (Gallus domesticus) Devi Moenek; Aven B Oematan
JURNAL KAJIAN VETERINER Vol 5 No 2 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i2.952

The presence of parasites in the backyard chicken body can cause damage to certain organs. Endoparasites can inhibit growth and lead to decreased production of chicken. The aims of this study is to identify the type of endoparasitic worms that infect backyard chickens as well as calculate the presence of endoparasit levels in backyard chicken feces. Samples of backyard chicken were taken randomly as many as 35 animals from farms in Kelapa Lima sub-district, Kupang City and Kupang Tengah sub-district Kupang District. The samples were examined by native and sediment method. The results showed that the endoparasites found in the method chickens were Ascarida galli, Raillietina sp. and Oxyuris sp. eggs, With a presence frequency of 88,58%, 5,71 %, and 5,71% respectively.

STUDI KASUS : PROFIL SEL DARAH MERAH ANJING YANG TERINFEKSI BABESIA SP. Yanse Yane Rumlaklak; Jois Moriani Jacob; Aven B Oematan
JURNAL KAJIAN VETERINER Vol 6 No 1 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i1.960

Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.

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