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All Journal JURNAL KAJIAN VETERINER
Nemay Anggadewi Ndaong
Bagian Farmakologi dan Toksikologi Fakultas Kedokteran Hewan Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health Veterinary

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Efek Pemaparan Deltamethrin pada Broiler Terhadap Aktivitas Enzim Alanin Aminotransferase, Aspartat Aminotransferase dan Gambaran Histopatologi Hepar Nemay Anggadewi Ndaong; Agustina Dwi Wijayanti; Sitarina Widyarini
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.990

Abstract

The purpose of this study is to determine the effects of deltamethrin exposure on the Broiler’s liver histopathological feature, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activity. Fourty DOC Broilers strain New Loghman are divided into four group of ten and they were adapted for 5 days prior to the treatment. Group I (KI) is a control group, group II were given 20 mg/L deltamethrin, group III were given 10 mg/L deltamethrin and exposure deltamethrin concentration 10 mg/L and group IV were given 5 mg/L delthametrin. Deltamethrin was mixed with drinking water and then was given to the treatment group for 30 days. Blood samples were taken on day 0, day 15 and day 30 of treatment to determine of ALT and AST enzyme activity. On day 35, all animal were sacrificed, liver were taken out and fixed in 10% of buffer formalin for microscopic examination. Results of the AST enzyme activity shows that exposure to 20 mg/L and 10 mg/L deltamethrin for 30 days resulted in the histopathological changes of the liver, such as, fatty degeneration, necrosis on liver cells, inflamation of the liver, and necrosis on liver cells without the infiltration of inflamation cells. It is conclude that exposure to 20 mg/L deltamethrin for 30 days resulted in an increase in AST enzyme activity which is supported liver histopathological changes: fatty degeneration, necrosis, inflamation, and necrosis on liver cells without the infiltration of inflamation cells.
Identifikasi Boraks, Formalin dan Kandungan Gizi serta Nilai Tipe pada Bakso yang Dijual di Lingkungan Perguruan Tinggi di Kota Kupang Thedyastry Pandie; Diana A. Wuri; Nemay Anggadewi Ndaong
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1003

Abstract

Penelitian ini bertujuan untuk mengidentifikasi penambahan boraks, formalin dan uji kandungan gizi serta TPC pada bakso daging sapi yang dijual di Lingkungan perguruan tinggi di Kota Kupang. Boraks dan formalin merupakan agen anti bakteri yang dapat menghambat pertumbuhan bakteri, dan apabila dicampurkan kedalam makanan dapat berbahaya bagi kesehatan karena boraks dan formalin bersifat karsinogenik. Lokasi pengambilan sampel dilakukan di kantin Universitas di Kota Kupang yaitu kantin Undana (2), kantin Unwira (3), kantin Politani (2) dan kantin PGRI (1), total sampel pengujian yaitu 8 sampel. Pengujian boraks dan formalin dilakukan pada laboratorium FKH Undana, dan diidentifikasikan menggunakan kit Easy Test®, pengujian kandungan gizi total protein kasar dan lemak kasar dilakukan di laboratorium Kimia dan Pakan Fakultas Peternakan Undana, serta pengujian TPC dilakukan pada laboratorium Bioreproduksi dan Kesehatan Ternak Fakultas Peternakan Undana. Hasil penelitian menunjukkan negatif untuk identifikasi boraks dan formalin. Berdasarkan SNI 01-3818-1995, standar yang ditetapkan untuk kandungan protein adalah minimal 9,0%, dan lemak maksimal 2,0%. Hasil pengujian protein menunjukkan 3 dari 8 sampel atau 37,5% sampel yang tidak memenuhi standar SNI. Hasil pengujian lemak terdapat 5 dari 8 sampel atau 62,5% sampel yang melebihi batas maksimum SNI. Berdasarkan SNI 01-3818-1995 batas cemaran mikroba pada bakso adalah 1x10³, dan pada hasil penelitian ini didapatkan bahwa terdapat 3 sampel dari 8 sampel atau sebanyak 37,5% sampel yang melebihi batas cemaran maksimum. Dari data diatas dapat disimpulkan, 2 dari 8 sampel atau 25% dari total sampel bakso daging sapi yang dijual di Lingkungan perguruang Tinggi di Kota Kupang aman dari bahaya boraks, formalin, dan kandungan gizi serta bahaya mikroorganisme.
Pengaruh Pemberian Ekstrak Etanol Daun Sambiloto( Andrographis paniculata Nees) Terhadap Histopatologi Pankreas Mencit ( Mus musculus ) Diabetes Melitus (DM) Tipe I Dwielma C. Nubatonis; Nemay Anggadewi Ndaong; Yulfia Nelymalik Selan
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1028

Abstract

Diabetes mellitus (DM) is a metabolic disorder which damage the beta cells (β) which led to the destruction of langerhans of pancreas gland. Diabetes mellitus is characterized by increased blood glucose levels and progressive changes to the structure of the histopathology of the pancreas. The purpose of this study was to determine the effect of ethanol extract of sambiloto leaf ( Andrographis paniculata Nees) on pancreatic histopathology of Alloxan induced diabetic mice. This research is an experimental research design by Post Test Randomized Control Group Design Experim ental animals used were mice ( Mus musculus) 12 sample of mice ±2 weeks old were adapted for 5 days. Mice were divided into 4 groups, namely, negative control, positive contro l (3 days of alloxan injection) and diabetic mice treated with the ethanol extract of sambiloto leaf with 2 different doses (doses I =2,2mg/kg and dosesII=4,4mg/kg) for 14 days. On day15, mice were necropsy and pancreas were collected for further Hematoxiline Eosin (HE) coloration. The results showed that DM alloxan induced type 1 mice (Mus musclus), the pancreas characterized by the occurrence of necrosis, langerhans islet was not bounded clear and breakthe linkage of the langerhans islet with a cinar while on ethanol extract therapy of sambiloto leaf dose 2,2 mg/kg and 4,4 mg/kg in mice DM type I showed the results an improvement on the langerhans islet. Based on these results, it can be concluded that a dose of 2,2 mg/kg and 4,4 mg/kg is effective dose to repair the structure of langerhans islet of mice Type I DM, but it is more adv isable to us a therapeutic dose of 4,4 mg/kg because it changes the structure of Langerhans islet.
Identifikasi Kandungan Logam Berat Kadmium (Cd) Pada Sapi Potong Yang Dipelihara Di Tempat Pembuangan Akhir (Tpa) Kecamatan Alak, Kota Kupang Timothy Kondanamu Nangkiawa; Annytha Ina Rohi Detha; Nemay Anggadewi Ndaong
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1030

Abstract

One of m ajor obstacle for cattle breeding enterprise in an urban area called Kupang city is the limited availability of grazing land . Attempting to overcome this , farmers in Kupang to utilize Alak final disposal (landfill) WITHOUT graz land of Alak. The purpose of this research was to identify the content of heavy metal cadmium (Cd) in cattle reared on waste/final disposal in Alak, Kupang . 12 blood samples were taken on the basis of various criteria , namely from the age group of 0 to 1.5 years of age; age group of 1.5 to 3 years and a group of more than 3 years. 3 ml blood samples were determined/ were measured by atomic absorption spectrophotometry in the Quality Testing Center and Animal Product Certification in Bogor. Results showed that the highest number was 0 .0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group over 3 years. Results showed that the highest number was 0.0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group ove r 3 years. In conclusion, 100% of heavy metal Cadmium was accumulated in beef cattle raised on Alak landfills and it might harmful to be consumed by people who live in kupang.
Co-Authors Agustina Dwi Wijayanti Annytha Detha Diana A. Wuri Dwielma C. Nubatonis Sitarina Widyarini Thedyastry Pandie Timothy Kondanamu Nangkiawa Yulfia N Selan