Titi Chasanah
Biology Faculty, Jenderal Soedirman University, Indonesia

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KARAKTERISTIK SALAK LOKAL BANYUMAS ( Salacca zalacca (Gaert) Voss) SEBAGAI UPAYA PELESTARIAN SPESIES INDIGENOUS Herawati, Wiwik; Chasanah, Titi; , Kamsinah
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Kajian taksonomi tentang karakteristik salak lokal Banyumas dengan tujuan untuk (1)Mendapatkan keragaman karakteristik morfologi salak lokal Banyumas. Dan (2) Mendapatkan hubungan kekerabatan antar kultivar salak lokal Banyumas berdasarkan karakteristik morfologi. Penelitian ini mengumakan metode survei dengan cara pengambilan sampel secara acak terpilih. Hasil penelitian menunjukan bahwa di Purwokerto terdapat 3 kultivar salak yaitu Salak ’kedung paruk’. Salak ’kalisube’ dan salak ’candinegara’ setelah dianalisis hubungan kekerabatan dengan UPGMA menggunakan metode MEGA 4.1 hasilnya salak’kalisube’mempunyai hubungan kekerabatan paling dekat dengan salak ‘candinegara’.
Effects of Some Feed Suplements Types to the Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial as Domestication Product Susatyo, Priyo; Sugiharto, Sugiharto; Hana, Hana; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5517

Abstract

Aquaculture can be improved by a diversified approach in the form of domestication research of a wild fish. The research has been done by cultivating Javaen Barb/Brek (Puntius orphoides) from Serayu river, Banyumas at a natural pond and laboratory scale. Brek fish is relative slow in growth and development of gonad. This research is aimed to determine: (1) the rate of general growth of Brek in the provision of some kinds of additional food; (2) Relative Growth Rate (RGR); (3) Specific Growth Rate (SGR) and Feed Conversion (FC). The research used experimental methods. The method was a completely randomized design (CRD) of 4 treatments and 3 replicationsof: A = 100% pellet; B = 70% soybean sprout and 30% pellet; C = 70% Ipomoea aquatica leaf and 30% pellet; D = 35% soybean sprouts, 35% I. aquatica leaf and 30% pellet. Quantitative data were analysed by analysis of variance (ANOVA) of the fishes growth.The results show influence of feed types to the Relative Growth Rate (RGR) of Javaean Barb. The best RGR with the value of 72.40 was given by feed types in the D combination. Meanwhile, Specific Growth Rate (SGR) and Feed Conversion (FC) were not affected by the feed types. The study were expected to provide suggestions for efficient feeding techniques in attempts at domestication of wild fish.How to CiteSusatyo, P., Sugiharto, S., Hana, H., & Chasanah, T. (2016). Effects of Some Feed Suplements Types to The Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial As Domestication Product. Biosaintifika: Journal of Biology & Biology Education, 8(3), 278-285.
Antioxidant Effect of Clorella vulgaris on Wistar Rat Kidney Induced by CCl4: A Histopathological Review Susatyo, Priyo; Rifanda, Achmad Akbar; Simanjuntak, Sorta Basar Ida; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13398

Abstract

Kidney is very susceptible to damage by toxicological compounds such as carbon tetrachloride (CCl­4). CCl4 produce free radicals, which cause lipid peroxidation and kidney damage and free radical release, which can be prevented by the administration of exogenous antioxidants, such as Chlorella vulgaris.The aim of study was to determine an effect of antioxidant of C. vulgaris on the histopathological features of Wistar rat  kidney which is induced by CCl4. Experimental study with completely randomized design. The variable was histopathology features of the kidneys. The doses of C. vulgaris extract were 3 mg, 4 mg, and 5 mg per 100 grams of rat body weight (BW). The administration of C. vulgaris extract was performed within 30 days, while the CCl4 induction (0.25 ml / 100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters were histopathology features of renal damage, proportion of tubular cell damage, and Bowmans space diameter. The results showed the administration of C. vulgaris extract was able to reduce the impact of damage caused by CCl4 (p<0.05). This was supported by histologic observations, which was showing a decrease of picnotic and vacuolated cells, normal brush border, and decrease of Bowmans space. In conclusion, 5 mg / 100 g BW of C. vulgaris extract can effectively protect the kidney from damage        caused  by        CCl4. The results of this study strongly support further research on immunostimulant content test C. vulgaris and determine the efficient dose for representative animals mammals also in humans.
Reproduction Characteristics of Rice Field Eel (Monopterus albus Zuieuw) on Several Functionally Changed Lands in Banyumas Regency Susatyo, Priyo; Setyaningrum, Nuning; Winarni, Elly Tuti; Chasanah, Titi; Atang, Atang
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.976 KB) | DOI: 10.11594/jtls.08.02.12

Abstract

Functional change of agricultural lands into non-agricultural lands will obviously have an impact on the dimin-ished or loss of habitat of the rice field eels (Monopterus albus Zuieuw). The aim of this research was to un-derstand the difference between (1) reproduction parameters (fecundity, gonadal maturity level (GML), and gonadal maturity index (GMI); (2) histological features of female and male gonadal development (oogenesis and spermatogenesis); (3) range of body length in each sexual phase; and (4) range of body length in each GML stage of captured eel from 4 sub-districts of Banyumas regency, Central Java (Ajibarang, Sumpyuh, Banyumas, and Sumbang). The total number of captured subjects were 631 eels; which consisted of 155; 227; 157; and 92 eels from Ajibarang, Sumpyuh, Banyumas, and Sumbang, respectively. We observed oogenesis in the stages of GML-1 (chromatin nucleolar stages and perinucleolar stage); GML-II (cortical alveolar formation stage or globular yolk stage), GML-3 (late globular yolk stage), GML-IV (mature or ripe stage), and follicle atretic stage. Also, we observed male gonadogenesis and spermatogenesis in the intersex. The range of GMI of captured eels was between 0.023 and 0.686. Eel in GML-I, GML-2, GML-3, and GML-IV had GMI within the range of 0.023-0.096; 0.096-0.425; 0.427-0.686; and >0.686, respectively. Eels achieved stage of GML-IV in body length range of 22.6-34.5 cm in Sumpyuh, 22.6-38.5 cm in Ajibarang, and 26.6-34.5 cm in Banyumas. There was no eel with the status of GML-IV in Sumbang, which had the most residential or functionally changed lands in its area. Based on these findings, can be concluded that the condition of rice fields or habitat of eel in functionally changed lands may affect eel’s growth and gonad maturation.