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All Journal Jurnal Sains dan Teknologi Farmasi Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology JSFK (Jurnal Sains Farmasi & Klinis) JFIOnline Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks
Yohannes Alen
Fakultas Farmasi Universitas Andalas
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Uji Sitotoksik Ekstrak dan Fraksi Daun Jati (Tectona Grandis Linn. F.) dengan Metoda Brine Shrimp Lethality Bioassay Alen, Yohannes; Akhsanita, Mardha; mulyani, Isna; Susanti, Meri
Jurnal Sains dan Teknologi Farmasi Vol 17 No 2 (2012)
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

he Study of cytotoxic effects of the methanolic extract and the fraction of Teak’s leave (Tectona grandis Linn. f.) by using Brine Shrimp Lethality Bioassay Method had been done. Results showed that the methanolic extract have cytotoxic level of (LC50) 16,58 ppm, n-hexane fraction 21,83 ppm, ethyl acetate fraction 21,31 ppm and residue fraction 21,27 ppm, respectively.
Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest Alen, Yohannes; Melati, Atika; Sarina, Gemmy; Djamaan, Akmal
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.816 KB) | DOI: 10.15416/ijpst.v5i2.15364

Abstract

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.
TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media Alen, Yohannes; Amelia, Rezki; Djamaan, Akmal
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.946 KB) | DOI: 10.24198/ijpst.v5i1.14644

Abstract

Antibiotic are secondary metabolites yielded by microbe especially fungus. Previous research succesfull screened four kinds of fungus that live in termites queen’s nest, one of them was Aspergillus flavus. Furthermore, Alen et al (2016g) states that this fungus last to produce metabolite compounds on SDA media which only exist in first and second  subculture extract, omit gradually for the next culture. It was presumed happen caused a different habitat to grow. So it is necessary to enrich the media with queen termite nest to get back the initial metabolites. The enrichment was done using four different media concentrations (0.25; 0,50; 0.75 and 1 grams of nest/mL media). The results show that enrichment of 1 gram of nest/mL media provides the most optimum fungus growht. The third subculture of Aspergillus flavus is cultured on enriched media which will become the fourth subculture, this fungus cultured until tenth subculture, do extraction and   fracination to each culture. Based on TLC profile analysis, the initial metabolite not yet formed until tenth subculture, but forms six new stain terpenoid compounds.  The result of  columns chromatography obtained 10 sub-faction. Activity test was done by diffusion method to 12  test bacteria and 3 test fungus. Spot 2,5,6 (Rf 0.84; 0,36; 0,26) inhibit the growth of Pseudomonas aeruginosa ATCC 27853, spot 3,4 (Rf 0.74; 0,52) inhibit the growth of Micrococcus luteus ATCC 10240. The use of enriched media affect formation of  secondary metabolites Aspergillus flavus.  Keyword : Secondary metabolites, Aspergillus flavus, Macrotermes gilvus Hagen., Enriched media, TLC Profiles, Activity Assay
Isolation and Activity Assay of Secondary Metabolites of Aspergillus niger in-Habiting in Termite’s Queen Nest Macrotermes gilvus Hagen., on Enriched Media Alen, Yohannes; Guslianti, Evi; Suharti, Netty
Indonesian Journal of Pharmaceutical Science and Technology Vol 6, No 1 (2019 In Press)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.342 KB) | DOI: 10.15416/ijpst.v5i3.15735

Abstract

The Fungus is a group of the microorganisms that produce secondary metabolites. Secondary metabolites of fungus may be changed in different media. Secondary metabolites from Aspergillus niger in habiting in termite’s queen nest Macrotermes gilvus Hagen are disappeared gradually in artificial media. It was the reason to enrich the media with termite’s queen nest. The purpose of this research was to obtain the similar secondary metabolites of Aspergillus niger as it grows in their habitat. Enrichment was done with the experimental method. It used three concentrations of nest 0.25; 0.75 and 1 g/mL Sabouraud Dextrose Agar (SDA) media. Isolation was done use chromatography method. The antibiotic activities against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 were performed by the diffusion method. Results showed that enrichment of nest 1 g/mL media gave the best growth of fungus and it obtain the similar secondary metabolites as it grows in their habitat. Three pure compounds, EG-13-31-2, EG-13-34-9, and EG-13-44-2 were obtained. Based on physicochemical data, all compounds were terpenoid class and one of them (EG-13-34-9) contain the phenolic group. All compounds have activity against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 the bacteriostatic category.Key words: Aspergillus niger; Macrotermes gilvus Hagen; Sabouraud Dextrose Agar (SDA); Isolation; Enrich Media; Diffusion Method
　　　RUBRAXHANTONE DARI Garcinia forbesii KING. DAN BIOAKTIVITASNYA Alen, Yohannes; Safitri, Novi; -, Dachriyanus; Ali, A. Manaf; Ladjis, N. H.; Sargent, M. V.
Jurnal Riset Kimia Vol 1, No 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.76

Abstract

 ABSTRACT  The rubraxhantone compound was isolated firstly from antimicrobial fraction of the bark of Garcinia forbesii King. (Famili Guttiferae) by chromatographic method. The structure of this compound was elucidated based on its spectral data properties. The isolated compound had a significant antimicrobial properties against the selected microbes by diffusion method.  Keywords : antimicrobial, Garcinia forbesii King., chromatographic, diffusion   method.
Aktivitas Antibakteri Fraksi Daun Aka Lambuang Alen, Yohannes; Rustini, .; Honesty, Resta
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.89 KB)

Abstract

Antibacterial activity tests were conducted on the leaf fraction of Aka lambuang (Merremia peltata (L.)Merr.) by dilution method and microplate reader towards Staphylococcus aureus and Escherichia coli. The results showed that activity performed at the minimum inhibitory concentration of 500 ppm and 1000 ppm respectivelyKeywords :  Merremia peltata, antibacterial, microplate reader.
Aktivitas Antibakteri Fraksi Daun Aka Lambuang Alen, Yohannes; Rustini, .; Honesty, Resta
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.89 KB) | DOI: 10.35617/jfi.v6i2.132

Abstract

Antibacterial activity tests were conducted on the leaf fraction of Aka lambuang (Merremia peltata (L.)Merr.) by dilution method and microplate reader towards Staphylococcus aureus and Escherichia coli. The results showed that activity performed at the minimum inhibitory concentration of 500 ppm and 1000 ppm respectivelyKeywords :  Merremia peltata, antibacterial, microplate reader.
　　　RUBRAXHANTONE DARI Garcinia forbesii KING. DAN BIOAKTIVITASNYA Yohannes Alen; Novi Safitri; Dachriyanus -; A. Manaf Ali; N. H. Ladjis; M. V. Sargent
Jurnal Riset Kimia Vol. 1 No. 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.76

Abstract

 ABSTRACT  The rubraxhantone compound was isolated firstly from antimicrobial fraction of the bark of Garcinia forbesii King. (Famili Guttiferae) by chromatographic method. The structure of this compound was elucidated based on its spectral data properties. The isolated compound had a significant antimicrobial properties against the selected microbes by diffusion method.  Keywords : antimicrobial, Garcinia forbesii King., chromatographic, diffusion   method.
TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media Yohannes Alen; Rezki Amelia; Akmal Djamaan
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.946 KB) | DOI: 10.24198/ijpst.v5i1.14644

Abstract

Antibiotic are secondary metabolites yielded by microbe especially fungus. Previous research succesfull screened four kinds of fungus that live in termites queen’s nest, one of them was Aspergillus flavus. Furthermore, Alen et al (2016g) states that this fungus last to produce metabolite compounds on SDA media which only exist in first and second  subculture extract, omit gradually for the next culture. It was presumed happen caused a different habitat to grow. So it is necessary to enrich the media with queen termite nest to get back the initial metabolites. The enrichment was done using four different media concentrations (0.25; 0,50; 0.75 and 1 grams of nest/mL media). The results show that enrichment of 1 gram of nest/mL media provides the most optimum fungus growht. The third subculture of Aspergillus flavus is cultured on enriched media which will become the fourth subculture, this fungus cultured until tenth subculture, do extraction and   fracination to each culture. Based on TLC profile analysis, the initial metabolite not yet formed until tenth subculture, but forms six new stain terpenoid compounds.  The result of  columns chromatography obtained 10 sub-faction. Activity test was done by diffusion method to 12  test bacteria and 3 test fungus. Spot 2,5,6 (Rf 0.84; 0,36; 0,26) inhibit the growth of Pseudomonas aeruginosa ATCC 27853, spot 3,4 (Rf 0.74; 0,52) inhibit the growth of Micrococcus luteus ATCC 10240. The use of enriched media affect formation of  secondary metabolites Aspergillus flavus.  Keyword : Secondary metabolites, Aspergillus flavus, Macrotermes gilvus Hagen., Enriched media, TLC Profiles, Activity Assay
Isolation and Activity Assay of Secondary Metabolites of Aspergillus niger in-Habiting in Termite’s Queen Nest Macrotermes gilvus Hagen., on Enriched Media Yohannes Alen; Evi Guslianti; Netty Suharti
Indonesian Journal of Pharmaceutical Science and Technology Vol 6, No 1 (2019)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.342 KB) | DOI: 10.24198/ijpst.v6i1.15735

Abstract

The Fungus is a group of the microorganisms that produce secondary metabolites. Secondary metabolites of fungus may be changed in different media. Secondary metabolites from Aspergillus niger in habiting in termite’s queen nest Macrotermes gilvus Hagen are disappeared gradually in artificial media. It was the reason to enrich the media with termite’s queen nest. The purpose of this research was to obtain the similar secondary metabolites of Aspergillus niger as it grows in their habitat. Enrichment was done with the experimental method. It used three concentrations of nest 0.25; 0.75 and 1 g/mL Sabouraud Dextrose Agar (SDA) media. Isolation was done use chromatography method. The antibiotic activities against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 were performed by the diffusion method. Results showed that enrichment of nest 1 g/mL media gave the best growth of fungus and it obtain the similar secondary metabolites as it grows in their habitat. Three pure compounds, EG-13-31-2, EG-13-34-9, and EG-13-44-2 were obtained. Based on physicochemical data, all compounds were terpenoid class and one of them (EG-13-34-9) contain the phenolic group. All compounds have activity against Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 10541 the bacteriostatic category.Key words: Aspergillus niger; Macrotermes gilvus Hagen; Sabouraud Dextrose Agar (SDA); Isolation; Enrich Media; Diffusion Method
Co-Authors . Dachriyanus A. Manaf Ali Akmal Djamaan Atika Melati Dwisari Dillasamola Evi Guslianti Fatma Sri Wahyuni Fitria Lavita Agresa Guslianti, Evi Hansen Nasif Honesty, Resta Honesty, Resta Isna Mulyani M. V. Sargent Mardha Akhsanita Melati, Atika Meri Susanti N. H. Ladjis Netty Suharti Novi Safitri Rezki Amelia Rezki Amelia Rustini Rustini Sarina, Gemmy Yori Yuliandra Yufri Aldi Zulhidayati Zulhidayati