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In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D Budisantoso, Iman; Amalia, Nurul; Kamsinah, Kamsinah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11018

Abstract

The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of Vanda sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from Vanda sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.
Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria Budisantoso, Iman; Indriani, Mona; Kamsinah, Kamsinah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.777 KB) | DOI: 10.15294/biosaintifika.v10i3.15718

Abstract

Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating  N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of  N. ampullaria microcutting. The purpose of this research was to determine the effect of  BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different)  test. The result showed that addition of BAP affected the growth of  N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at  0.57 ppm.
EFFECT CADMIUM EXPOSURE ON RENAL FUNCTION OF WELDING WORKERS hernayanti, hernayanti; Santoso, Slamet; Lestari, Sri; Prayoga, Lucky; Kamsinah, Kamsinah; Rochmatino, Rochmatino
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 11 No 1 (2019): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.116 KB) | DOI: 10.20884/1.ki.2019.11.1.1422

Abstract

ABSTRACT Cadmium is one of the heavy metals that has nephroxic effect and &nbsp;dangerous to &nbsp;human health, especially welding workers. If cadmium enters the body will bind to metallotionein protein.&nbsp; This process can increase the formation of free radical compounds, thereby induced lipid peroxidation which damage kidney organ and may cause impaired the renal function. Decreased renal function is characterized by an increase of ?2-Microglobulin and creatinine level. The aim of this study are to know effect of Cd exposure to renal function indicated by increasing of Cd, creatinine and ?2-M level. Research design used case control. Research subjects consist of thirty welding workers in Purwokerto with a period of working more than 3 years as case subjects and control subjects come from Pamijen village Baturaden. &nbsp;Data of Cd, creatinine and ?2-M level were analyzed by t independent. Result of this research showed that in case subjects occur an increase of Cd, creatinine and ?2-M level and have higher level compare with control subjects.It can be concluded that Cd exposure has an effect on renal disfunction which indicated by increasing&nbsp; blood Cd level, creatinine and ?2-M level. &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;
CADMIUM (CD) ABSORPTION AND PHENOL CONTENT IN POGOSTEMON EXPOSED TO HEAVY METALS Proklamasiningsih, Elly; Budisantoso, Iman; Kamsinah, Kamsinah
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.02

Abstract

Patchouli (Pogostemon cablin Benth.) is an important plant used by industrial facilities to absorb cadmium (Cd) in polluted land. We performed an experiment using plant medium polluted with both Cd and lead (Pb) with added humic acid. The aims of this study were to 1) determine the effects of humic acid in growth medium contaminated with Cd and Pb on the absorption of Cd and phenol content in patchouli, and 2) determine the Cd tolerance level of the growth media. A completely randomized factorial design was used for the experiment with two factors. The heavy metals were a combination of pure PbNO3 and Cd (PC) with a ratio 1 : 1, and included five concentrations: PC0 (without PbNO3 and without Cd); PC1 (250 ppm PbNO3 + 250 ppm Cd); PC2 (500 ppm PbNO3 + 500 ppm Cd); PC3 (750 ppm PbNO3 + 750 ppm Cd); PC4 (1,000 ppm PbNO3 + 1,000 ppm Cd) and humic acid concentration (0; 6,000; 12,000; and 18,000 ppm). Each treatment was replicated three times. The parameters observed were plant biomass, Cd absorption, and phenol content. The application rate of humic acid to the plant medium containing heavy metals influenced the growth of patchouli, Cd absorption, and phenol content. An application rate of 12,000 ppm of humic acid reduced the toxicity of the heavy metals and increased the dry biomass and phenol content of patchouli.
Developing English Printed Material on Theme of Plant for Early Young Learners at Paud Terpadu Joy Kids Makassar Hilalia, Nur; Kamsinah, Kamsinah; Nurpahmi, Sitti
Journal of English Teaching and Learning Issues Vol 3, No 2 (2020): December 2020
Publisher : Institut Agama Islam Negeri Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/jetli.v3i2.8582

Abstract

Based on a preliminary study conducted in PAUD Terpadu Joykids Makassar, it was found that the material provided for students in introducing English vocabulary based on theme is still limited. In this case, the researcher intended to develop thematic English material for students. This study was Research and Development adapting ADDIE model that consists of Analysis, Design, Development, Implementation and Evaluation. In developing the material the researcher concerned on developing “plant” theme material. There were 1 teacher, 2 experts and 20 students involved in this research. This study found out the need of students at PAUD Terpadu Joykids over English material. The data of students need then become the inventory need in designing and developing the material. Moreover, the material consisted of English vocabulary integrated with the theme, number, picture, simple sentence, and artwork.
Developing ELT Material on Theme of Plant for Early Young Learners at PAUD Terpadu Joy Kids Makassar Hilalia, Nur; Kamsinah, Kamsinah; Nurpahmi, Sitti
Journal of English Teaching and Learning Issues Vol 3, No 2 (2020): December 2020
Publisher : Institut Agama Islam Negeri Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/jetli.v3i2.8582

Abstract

This Study aims at designing English material at PAUD Terpadu Joy Kids Makassar. A preliminary study conducted in PAUD Terpadu Joykids Makassar shows that the material for students in Intorducing English vocabulary based on theme is still limited. The material consists only one page of vocabulary without any other kind of activity that might encourage and facilitite them in recognizing the English vocabulary. In this case the researcher intended to develop thematic English material for students.This study was Reserach and Development adapting ADDIE Model that consists of Analysis, Design,Development,Implementation and Evaluation. In developing the material the researcher concerned on developing "Plant" theme material. There were 2 teacher, 2 experts and 20 students involved in this research.This study found out the need of students at PAUD Terpadu Joy kids over English material. The data of students need then become the inventory need in designing and developing the material. Moreover, the material consisted of English vocabulary integrated with the theme,number,picture,simple sentence and art work.
Language Empowering in Character Building (Pemberdayaan Bahasa dalam Pembentukan Karakter) Kamsinah Kamsinah
JURNAL ARBITRER Vol 1, No 1 (2013)
Publisher : Masyarakat Linguistik Indonesia Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/ar.1.1.48-57.2013

Abstract

Character building is the most important thing to do as it is a striving system which underly behavior (Freud).  Even more, good character is more to be praised than outstanding talent. Most talents are to some extent a gift. Good character, however, is not given to us. we have to build it peace by peace by thought, choice, courage, and determination.  So important it is that it is said that  if there is no more character every thing is lost (Mahatma Gandhi). The best way to build it is  to develop   the function of all  individual potential,  including cognitive, affective,  and psychomotoric aspects simultanously in the context of socio-cultural interaction (in family, in  school, and in society). Character  is gained by nature and nurture. It can be done begin from the golden age to the old one through the three character building components: moral knowing, moral feeling, and moral action as suggested by Lickona. They make it possible since human beings, as  the best-formed creature of all, are the ones and the only creature posessing culture, and that,  they can educate and be educated in terms of   the model of  person of character.  Everybody must have character. Therefore,  to apply Lickona’s,  one must  empower her/his language, in which  she/he/ perform her/his competence in using language creativity (Chomsky) in   both ordinary  and literary language. In this case, Buginese language is used as the sample.
FOLLOW THE EVENTS IN THE INDONESIAN LANGUAGE EXPRESSIVE SAID TRANSACTION IN THE TRADITIONAL MARKET TOWN OF BAUBAU Harziko Harziko; Ikhwan M. Said; Kamsinah Darwis
JURNAL ILMU BUDAYA Vol. 6 No. 1 (2018)
Publisher : Universitas Hasanuddin

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Abstract

Expressive speech acts are often used in both environments, schools, offices, hospitals and market. This research aims to describe the function of speech is expressive of sellers and buyers in the event of transaction in the traditional market town of Baubau. This qualitative descriptive nature of the research. Sampling is done in purposif. Linguistic phenomena is examined with a pragmatic approach. Research data in the form of verbal data sourced from the conversation of sellers and buyers in a transaction. The data was collected by using the method of observation through the technique of record and note. The data have been analyzed in qualitative descriptive are classified. The results showed that the functions of the seller's expressive speech consists of functions to thank, praise, and function function complained. Furthermore the functions of speech is expressive of the buyer, i.e. the function to thank, praise, function and the function of complaining.
TRANSITIVITY AND IDEOLOGY IN DONALD TRUMP CAMPAIGN SPEECH Yuliana Yuliana; L. M. Manda; Kamsinah Darwis
JURNAL ILMU BUDAYA Vol. 6 No. 1 (2018)
Publisher : Universitas Hasanuddin

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Abstract

Political speech is often being a topic of research in linguistic. Some of interdisciplinary studies appeared while the role of the language has developed. They are pragmatics, critical discourse analysis, phsycholingustics, neurolinguistics and sociolinguistics. This research focused on the transitivity system and ideology in Donald Trump Speech Campaign. Transitivity System that stated by Halliday become a tool to analyze the linguistic data which this theory has been used by Fairchlough in his critical discourse analysis. Based on the aims of this research that described transitivity system and ideology of Donald Trump speech campaign, therefore this research called descriptive research. Method of collecting data is method of seeing and noted technique. Technique purposive sampling is used in this research. The result showed that there are six types of process is used by Donald Trump to represent his experience, those are material process, relational process, mental process, verbal process, behavioral process, existential process. Material process is mostly used by Donald Trump followed by relational process and then mental process. To show his ideas, Donald Trump used material process mostly and showed his principal that better do action than only talk. To draw attention of US society and influenced their thought, Donald Trump is often used rasism issue in his speech campaign such as limit the access of immigrant in working and list the muslims US in separated database. Beside that, some of his statements showed that he is a narsistic. He showed his love and proud of himself mostly and he has protectionism in economy. 
SUMBANGAN BAHASA INDONESIA TERHADAP PEMEKARAN KOSAKATA BAHASA BUGIS: KASUS PENGGUNAAN BAHASA BUGIS PADA MEDIA SOSIAL FACEBOOK (The Contribution of Indonesian Language on Buginese Vocabulary Development: A Case Study of the Use of Buginese on Facebook Social Media) Muhammad Darwis; Kamsinah Kamsinah
SAWERIGADING Vol 25, No 2 (2019): Sawerigading, Edisi Desember 2019
Publisher : Balai Bahasa Sulawesi Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.74 KB) | DOI: 10.26499/sawer.v25i2.626

Abstract

AbstrakThe aim of this research is: (1) to identify the forms and categories of Indonesian words that are absorbed into Buginese sentences and (2) to reveal the reasons for the use of Indonesian elements into Buginese sentences by Facebookers in the social media. Data on this qualitative research obtained from social media ‘Facebook’. The data source of this research is the Facebookers who are members of the MABBASA UUGIE KU PESBU’ group, November 2013 to April 2014 edition. Data analyzed are Buginese sentences consisting of three to five examples of Buginese sentences containing Indonesian elements in the form of words, phrases or clauses taken purposively. Furthermore, the analysis was carried out with grounded research strategies. The results of this research indicate that (1) Buginese language can survive as a means of communication within Buginese ethnic groups when writing on the social media ‘Facebook’, due to they have obtained vocabulary contributions from Indonesian in the form of the basic word, affixation word, and phrase. In word categorization, the loan words consist of nouns, verbs, adjectives, and conjunctions. Then, (2) the use of the Indonesian language elements has four main reasons, namely (a) filling in the blanks, (b) adding equivalence variations, (c) clarifying the meaning, and (d) interference. Reasons (a) to (c) can take the form of code-mixing and code-switching. AbstrakPenelitian ini bertujuan: (1) mengidentifikasi bentuk dan kategori kata bahasa Indonesia (bI) yang  terserap ke dalam kalimat-kalimat bahasa Bugis (bB) dan (2) mengungkap alasan-alasan penggunaan unsur-unsur bI tersebut ke dalam kalimat bB oleh para Facebooker di media sosial. Data penelitian kualitatif ini diperoleh dari media sosial Facebook. Sumber data penelitian ini ialah para Facebooker yang menjadi anggota grup MABBASA UUGIE KU PESBU’ edisi bulan November 2013 s.d. bulan April 2014. Data yang dianalisis ialah kalimat-kalimat ber-bB yang terdiri atas tiga sampai lima contoh kalimat ber-bB yang berisi unsur-unsur bI, yang berupa kata, frasa, atau klausa, yang diambil secara purposif. Selanjutnya, analisis dilakukan dengan upaya grounded research. Hasil penelitian ini menunjukkan bahwa (1) bB dapat bertahan hidup sebagai sarana perhubungan intern suku Bugis dalam komunikasi tulisan sosial Facebook karena memperoleh sumbangan kosakata bI yang berbentuk kata dasar, kata berimbuhan, dan frasa atau ungkapan. Dari segi kategorisasi kata, unsur-unsur serapan tersebut terdiri atas kata benda, kata kerja, kata sifat, dan kata sambung. Kemudian, (2) penggunaan unsur-unsur bI tersebut memiliki empat alasan utama, yaitu (a) mengisi kekosongan, (b) menambah variasi kesepadanan, (c) memperjelas pemaknaan, dan (d) interferensi. Alasan (a) sampai dengan (c) dapat mengambil bentuk campur kode dan alih kode.