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sujarot dwi sasmito, sujarot dwi
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Endoglin Expression and the Level of Tgf-Î’ Are Increased In the Placental Tissue and Correlated with Low Fetal Weight in Malaria Infected Mice sasmito, sujarot dwi; Ulfiati, Adilah; Wardana, Ardhian; Nugraheni, Fitriana; Pradiptasari, Nur Fahma; Zulaifa, Zakiyah; Norahmawati, Eviana; Sardjono, Teguh Wahju; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.5.1.%x

Abstract

Malaria infection during pregnancy can cause accumulation of infected-red blood cells in placental intervillous space and induces placental tissue inflammation and hypoxia. This condition triggers endoglin expression, and release of soluble endoglin which can interfere TGF-β binding to its receptor. The aim of this study was to investigate the correlation between placental endoglin expression and TGF-β level with low fetal weight (LFW) in malaria-infected mice. Nine pregnant mice infected with Plasmodium berghei on the day 9th post mating (malaria infected group) and eight normal pregnant mice (non-infected group) were used in this study. The mice were sacrificed on the day 18th post mating, and all fetal body weights were measured by analytical scale. Enzyme Link Immunosorbent Assay (ELISA) was done to determine the level of placental TGF-β while immunohistochemical staining was performed to examine endoglin expression in placental tissue. The mean of fetal body weights of malaria infected group was significantly lower than non-infected group (p=0,002), while the expression of placental endoglin in malaria infected group was significantly higher than non-infected group (p=0.003). The level of placental TGF-β in malaria infected group was also higher than non-infected group but the difference was not significant (p=0.064). Pearson correlation test showed that there were significant negative correlations between fetal body weights with the level of placental TGF-β (p=0.017, r=-0.568) and the expression of placental endoglin (p=0.002, r=-0.694). Malaria infection in pregnant mice will increase both TGF- β and endoglin in placenta tissue and correlate with low fetal weight.   
Excess Fibrin Deposits Decrease Fetal Weight of Pregnant Mice Infected by Plasmodium berghei Andari, Desy; Rahma, Zainabur; yuliyanik, Yuliyanik; Sasmito, Sujarot Dwi; Mintaroem, Karyono; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Low birth weight is commonly attributed to malaria in pregnancy, but the cellular and molecular mechanisms that underlie this poor birth outcome are incompletely understood. A universally described histopathological feature of placental malaria is excessive deposition of fibrin, the end-product of the coagulation cascade. This study was conducted to compare fibrin deposit in pregnant mice that infected by Plasmodium berghei (treatment group) to the normal pregnant mice (control group) and its association with fetal weight. This research is in vivo experimental laboratory study that used 18 pregnant Balb/c mice which divided to the control the group (8 mice) and treatment group (9 mice infected by P.berghei). Placentas were staining with Haematoxylin-Eosin (HE) for fibrin deposits examination whereas fetal weight was performed with Mettler analytical balance AE 50. Fetal weight of the treatment group was lower than those of the control group (t test, p=0,002). Fibrin deposits were increased in the treatment group (t test, p=0,005) and influenced weight of fetuses (Spearman r=-0,586, p=0,014). Weights of fetuses are interfered by fibrin deposits during malaria infection.
Co-Authors Adilah Ulfiati, Adilah Ardhian Wardana, Ardhian Desy Andari Eviana Norahmawati Fitriana Nugraheni, Fitriana Karyono Mintaroem Loeki Enggar Fitri Nur Fahma Pradiptasari, Nur Fahma Rahma, Zainabur Teguh Wahju Sardjono Yuliyanik yuliyanik Zakiyah Zulaifa, Zakiyah