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All Journal Jurnal Ilmiah Farmasi INDONESIAN JOURNAL OF PHARMACY JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Rochmy Istikharah
Department Of Pharmacy, Faculty Of Mathematics And Natural Sciences, Universitas Islam Indonesia, Yogyakarta, Indonesia
Medicine & Pharmacology Public Health

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Influence of fermented horse milk supplementation on immunity against hepatitis A vaccine in Balb/c mice Sasmito, Ediati; ., Rumiyati; W., Sri Rahayu; Andriyani, Evie; Istikharah, Rochmy
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 1, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.533 KB) | DOI: 10.14499/indonesianjpharm0iss0pp13-18

Abstract

It has been evaluated the influence of fermented horse milk into Balb/c mice on immunity against hepatitis A vaccine. The study employed a complete randomized design using thirty mice which were divided into 3 groups. Each group orally consumed fermented horse milk at dose 0.4 mL/20g body weight, levamisole (as positive control) at dose 0.4 mL/0.45 mg/20g body weight and distilled water (as negative control) at dose 0.4 mL/20g body weight respectively on a daily basis in addition to the standard diet for 46 days. The mice were immunized intraperitoneally (3 times) at d 7, d 28, and d 42, with 0.65 μg/20g body weight of hepatitis A vaccine. Blood samples were collected from plexus retroorbitalis using heparinized capillary one week after first (d 14), second (d 35) and 4 days (d 46) after third immunizations, and then mice were sacrificed to isolate the lymphocytes. The IgM, IgG and IgA titers in the sera and the culture media were measured by ELISA.The IgM (except d 46), IgG (subtype IgG1 (except d 14), IgG2a, IgG2b, IgG3 (except d 35), and IgA titers in the sera significantly higher (p<0.05) than controls. This difference was associated with enhanced in vitro antibodies secretion by cultured lymphocytes isolated from the spleen for IgA. The result suggested that orally fermented horse milk supplementation inhances immunity against hepatitis A vaccine in Balb/c mice. The SDS-PAGE showed that 30-kDa proteins were present in fermented horse milk as well as fermented cow milk, but 7.4-kDa protein present only in fermented horse milk.Key words : fermented horse milk, immunity, hepatitis A vaccine, Balb/c mice.
PENGARUH PEMBERIAN SUSU KUDA FERMENTASI TERHADAP ANTIBODI IMUNOGLOBULIN A (IgA) MENCIT SETELAH VAKSINASI HEPATITIS A Rochmy Istikharah; Ediati Ediati; Rumiyati Rumiyati
Jurnal Ilmiah Farmasi Vol. 2 No. 1 (2005)
Publisher : Universitas Islam Indonesia

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ABSTRACTIgA-deficient in individuals have developed mucosal infections, atopy, autoimmune diseasesand rheumatic. Under the circumstance, the development of an immunomodulator that stimulatesimmune system, especially IgA, is truly desired. The research is objected on finding out the effect offermented horse milk treatment on IgA antibody in Hepatitis A immunized mice. IgA concentrationin serum and lymphocytes was evaluated using indirect ELISA method. Fermented horse milkgroup was compared with water group to calculate the percentage of IgA concentration increase.Univariate analysis was used to analyze Optical Density (OD) product from ELISA. The resultshowed that fermented horse milk increased 46.20 % IgA concentration in serum and 95.47 % inspleen lymphocytes. Univariate analysis showed that fermented horse milk increased significantlyIgA spleen lymphocytes but not IgA serum.Keywords : immunomodulator, IgA, hepatitis A vaccine, ELISA
Aktivitas antioksidan ekstrak etanol daun Sonchus arvensis L. Rochmy Istikharah; Khittah Dea Annisa Annisa; Nofran Putra Pratama; Hady Anshory  Tamhid
Jurnal Ilmiah Farmasi Vol. 11 No. 2 (2015): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

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Intisari Penelitian terhadap ekstrak air dan ekstrak metanol daun Sonchus arvensis L. atau tempuyung telah terbukti mempunyai khasiat sebagai antioksidan. Salah satunya diduga karena kandungan flavonoidnya. Etanol merupakan pelarut yang paling sering digunakan dalam proses ekstraksi dan mempunyai kemampuan yang baik dalam mengekstraksi flavonoid. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun S. arvensis sebagai antioksidan dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) dan metode ABTS (2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid). Ekstrak daun S. arvensis diperoleh melalui proses maserasi dengan etanol 70%. Uji aktivitas antioksidan dilakukan dengan menggunakan DPPH dan ABTS sebagai sumber radikal bebas terhadap seri kadar ekstrak sehingga dapat dihitung nilai IC50 melalui perhitungan PROBIT. Seri kadar vitamin C digunakan sebagai kontrol positif. Hasil uji peredaman radikal bebas menunjukkan nilai IC50 S. arvensis sebesar 138,26 µg/mL terhadap ABTS dan 64,97 µg/mL terhadap DPPH. Ekstrak etanol daun S. arvensis yang diuji mempunyai aktivitas antioksidan. Kata kunci : Sonchus arvensis, antioksidan, ABTS, DPPH
Nanocurcumin Preparation for Reducing Vcam-1 and IL-6 in High Fat Diet-Induced Hyperlipidemic Rats Dimas Adhi Pradana; Maulana Ardhi; Arifa Caryn Dea Utami Hasyono; Dzihni Meytasari; Farah Deastasa Nabilah; Rochmy Istikharah; Lutfi Chabib
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1047.714 KB) | DOI: 10.14499/indonesianjpharm30iss1pp58

Abstract

Hyperlipidemia is a pathological condition due to lipid metabolism abnormalities. Increased and oxidized LDL in hyperlipidemia will trigger an inflammatory response and produce proinflammatory cytokines, such as vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Curcumin can be used as antioxidants, anti-inflammatory and antihyperlipidemia, but as it is practically insoluble in water, formulation of curcumin nanosuspension is made to improve the effects of curcumin therapy. This study was conducted to determine the activities of nanocurcumin preparation as preventive measures for rats induced with hyperlipidemia. The test animals used were 49 male Wistar rats divided into 7 test groups: normal control group, negative control group, 80mg/kg BW/day curcumin control group, and nanosuspension curcumin dose of 80mg/kg BW/day, nanoemulsion curcumin dose of 80mg/kg BW/day, SNEDDS curcumin dose of 80mg/kg BW/day and solid lipid nanoparticles dose of 80mg/kg BW/day. Provision of preventive measure was performed on days 1-67. On day 11-67, the induction of lard and egg yolk (1:1) was administered with a volume of 2mL/200g of rat BW. On the 68th day, blood samples were taken for the determination of VCAM-1 and IL-6 parameters using Biotin-Streptavidin-Amplified Enzyme-Linked Immuno-sorbent Assay method. Data analysis of VCAM-1 and IL-6 levels between test groups were conducted by means of normality test and One-way ANOVA (p<0.05). Based on the data of VCAM-1 and IL-6 levels, nanocurcumin dose of 80mg/kg BW preparation was able to significantly improve the preventive activity of curcumin compared to curcumin suspension dose of 80mg/kg BW by reducing VCAM-1 levels in SNEDDS (53.260%), nanoemulsion (52.737%), nanosuspension (52.325%) and solid lipid nanoparticles (51.444%) and decreasing IL-6 levels in SNEDDS (33.030%), nanoemulsion (31.568%), nanosuspension (29.898%), and solid lipid nanoparticles (28.875%).
Characterization, formulation and evaluation of glibenclamide with ß-cyclodextrin inclusion complexes tablets Yandi Syukri; Farida Ulfa; Asih Lestari; Lelita Ayu Saputri; Rochmy Istikharah; Aris Perdana Kusuma
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 9, No 3, (2018)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol9.Iss3.art3

Abstract

Background: Glibenclamide is an oral antidiabetic drug which is practically insoluble in water. Formation of ß-cyclodextrin inclusion complex was able to increase glibenclamide solubility. Objective: Aims of this study are to characterize, formulate and evaluate  inclusion complex tablets of glibenclamide to meet the requirements in Pharmacopeia.Methods: Inclusion complex was prepared in a 1: 1 and 1: 2 molar ratio  by spray drying method. Characterization were performed by using Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscope (SEM). Further, it was formulated into tablets by direct compression technique using primojel and crospovidone as disintegrants.   Uniformity weight, hardness, friability, disintegration, and tablets were evaluated include dissolution. Dissolution studies of inclusion complex were performed by using United States Pharmacopeia (USP) II apparatus.  Drug concentration dissolution was determined with high pressure liquid chromatography (HPLC).Results: Result of FTIR and SEM provided evidence of  glibenclamide and ß-cyclodextrin complex formation after using spray drying methods. The tablet evaluation with primojel and crospovidone as disintegrant showed that increase concentration of disintegrant would increase disintegration time of the tablets. All of the formulas meet the requirements in the Pharmacopoeia.Conclusion: The inclusion complex of glibenclamide–ß cyclodextrin successfully used for enhancing the solubility of glibenclamide. The tablets meet the requirements in Pharmacopeia.
Co-Authors Arifa Caryn Dea Utami Hasyono Aris Perdana Kusuma, Aris Perdana Asih Lestari Dimas Adhi Pradana Dzihni Meytasari Ediati Ediati Ediati Sasmito Evie Andriyani, Evie Farah Deastasa Nabilah Farida Ulfa Khittah Dea Annisa Annisa Lelita Ayu Saputri Lutfi Chabib, Lutfi Maulana Ardhi Nofran Putra Pratama Rumiyati ., Rumiyati Rumiyati Rumiyati Sri Rahayu W., Sri Rahayu Tamhid, Hady Anshory Yandi Syukri