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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production

Wirdateti Wirdateti, Wirdateti

Pusat Penelitian Biologi LIPI, Jl. Raya Jakarta-Bogor, Cibinong 16911

Author-ID : 587105

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary Other

Published : 18 Documents Claim Missing Document

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KUALITAS KRIOPRESERVASI SEMEN RUSA BAWEAN {Axis kuhlii (Temminck, 1836)} HASIL PENANGKARAN [Quality of Cryopreserved Semen of Captive Bawean Deer {Axis kuhlii (Temminck, 1836)}] Wirdateti, Wirdateti; Nugraha, R Taufiq P; Semiadi, G; Widyastuti, SK; Yulianto, Yulianto
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bawean Deer {Axis kuhlii (Temminck, 1836)} is an endemic deer species from Bawean island, Indonesia. Under the National Protection act, the species is listed as protected and in the IUCN is categorized as Critically Endangered. In order to maintain the existence of these species, wildlife conservation of germplasm is needed through development of technique of sperm cryopreservation for Bawean deer semen. The sperm were collected from captive animals in Station for Beef Cattle Research Center, Ministry of Agriculture, at Grati, Pasuruan, which consisted of two adult males and two sub-adult males. The research objective was to determine the quality and quantity of preserved sperm from captive deer Bawean in the form of frozen semen for conservation needs and further usage. Prior to sperm collections, the animal was anesthetized with mixture of xylazine and ketamin. Sperm was then collected by an electroejaculator using a 2-cm probe diameter and 17 cm of length. Extender solution used was tris glycerol. The semen was able to be collected only from two adult stags with sperm motility of 50-60% and sperm concentration was in the range of 500-1140 x 106sel/ml. This study suggested the need for monitoring the developmentof sperm in relation to antler development.

Variasi Genetik pada Rusa Sambar (Rusa unicolor) di Penangkaran, Kabupaten Penajam, Kalimantan Timur Wirdateti, Wirdateti; Brahmantyo, Bram; Semiadi, Gono; Reksodihardjo, Andi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Sambar deer (Rusa unicolor) is the largest Indonesian deer species. The largest population of captivation is located at Penajam district, East Borneo (UPTD). First population was introduced in 1990 with four individuals. The use of molecular marker was aimed to identify and characterise the level of genetic diversity within the UPTD population as well as to identify a possible of botolneck population genetic status. This study discussed the relevance of the result for management purposes of captivation. The results indicated that sambar deer populatin (n=38) had an average genetic distance (d) in population as 0.006 with nucleotide diversity (Ï€) being 0.0159). A total of 43.48% of the population was homogeneous that showed no nucleotide differences among individuals.

Observations on Natural Foods and Nutrition Content of Critically Endangered Turtle (Leucocephalon yuwonoi) in Central Sulawesi Riyanto, Awal; Wirdateti, Wirdateti; Soemarno, Suprayogo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Kura-kura Sulawesi (Leucocephalon yuwonoi) adalah satu dari dua jenis kura-kura endemik Sulawesi dengan penyebaran yang terbatas. Sejauh ini data natural historinya sangat minim, padahal sangat dibutuhkan dalam upaya penangkaran satwa yang berstatus kritis IUCN ini. Penelitian ini bertujuan untuk mengungkap tentang pakan di alam beserta kandungan nutrisinya. Survei lapangan dilakukan di kawasan Bangkir dari tanggal 23 Mei sampai 5 Juni 2004 dan di Moutong dari tanggal 11 sampai 19 Oktober 2004. Data pakan di alam diperoleh dari analisis sampel feces dan hasil wawancara kepada para pemburu kura-kura beserta pengumpul. Kepastian nama jenis pakan diperoleh dengan melakukan identifikasi sampel tumbuhan pakan di Herbarium Bogoriense. Data kandungan nutrisi diperoleh melalui analisis proksimat sampel pakan yang terkoleksi selama survei. Hasil penelitian ini menunjukkan bahwa terdapat 32 jenis tumbuhan yang jadi sumber pakan di alam, variasi kandungan nutrisi yang cukup besar, kandungan lemak rendah berkisar antara 0.74 - 8.33% (2.41+2.03) dari berat kering. Berdasarkan dominansi, keberadaan di habitat (multiple season) dan tingginya kandungan energi diduga kuat bahwa Colocasia esculenta, Limnocharis flava and Ipomoea aquatica merupakan pakan utama di alam.

KONSUMSI DAN EFISIENSI PAKAN PADA KUKANG (NYCTICEBUS COUCANG) DI PENANGKARAN Wirdateti, Wirdateti; Puspitasari, Dewi; Diapari, Didid; Tjakradidjaja, Anita S
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3474

ABSTRACTFeed Intake and Efficiency of Slow Loris (Nycticebus coucang) in Captivity. Study on feed intake and efficiency of captive slow loris (Nycticebus coucang) has been conducted at the Division of Zoolgy, Research Center for Biology ? LIPI for five weeks. Three individuals were placed in three cages, with its sizes of 1.2 x 1.7 x 1.9 m (one cage) and 0.8 x 1.7 x 1.9 m (two cages). Feeds consisting of papaya, banana, coconut, passion fruit, guava, sweet corn, quail egg, and white bread, were given ad libitum. Average body weight of slow loris in the beginning of the study was 587 gram/head and at the end was 648.33 gram/head. Average of feed consumption was 317.26 gram/kgBW/day. Average of feed efficiency and protein efficiency ratio (PER) was 0.0270 and 0.0945, respectively.Key words: Feed, slow loris, Nycticebus coucang, captivity, feed efficiency, PER.

KERAGAMAN GENETIK RUSA SAMBAR (RUSA UNICOLOR), PEMANFAATAN DAN IMPLIKASINYA UNTUK KONSERVASI Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3071

Genetic Diversity of Sambar Deer (Rusa unicolor) in Utilization and Implications for Conservation. The deer sambar (Rusa unicolor) is one of species of Genus Rusa, was widely distributed in South Asia antil South East Asia. This species in Indonesia was widely distributed in Kalimantan and Sumatra island, but now, the hunting, poaching and habitat loss have reduced its populations drasstically. In order to provide useful information for its conservations. The genetic diversity and population structure of the wild sambar deer was observed by analyzing the 962 bp long of fragment mitochondrial DNA (mtDNA) control region (D-loop)fragment. The result detected, twenty different haplotypes from 22 samples from Borneo and Sumatra. Overall, sambar deer have a relatively high genetic diversity compared to other the ceervid species, with a haplotype diversity (h) 0.9870 and nucleotide diversity (ð) 2.931±0.260%.The genetic distance of Borneo populations (East Borneo) higher compared with Sumatra?s populations that is d = 0.018±0.003 on Borneo and d = 0.009±0.002 on Sumatra. The structure of phylogenetic tree showed that two populations were separate based on the haplotypes differences.Keywords: conservation, control region, genetic diversity, sambar deer.

ANALISIS SEKUEN DNA MITOKONDRIA CYTOCHROME OXIDASE I (COI) MTDNA PADA KUKANG INDONESIA (NYCTICEBUS SPP) SEBAGAI PENANDA GUNA PENGEMBANGAN IDENTIFIKASI SPESIES Wirdateti, Wirdateti; Indriana, Eka; Handayani, Handayani
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2322

Slow lories is small size primates of the genus Nycticebus that are in endangered status. Habitat pressure andhigh trade as pet animals has made the population decline both quantitatively and qualitatively, therefore it isnecessary to save the slow lories through conservation action. Morphologically, the individuals are difficult tobe distinguished among the species, therefore making much harder in identification for conservation purposessuch as from confiscated individuals. Therefore molecular marker is needed through mitochondrial DNA usingCOI gene sequences analysis is needed for identification. This study used 24 samples of slow lories consistedof 2 from Kalimantan, 9 from Java and 13 from Sumatra. The result indicated that in the 660 bp there were 62variable sites with 15 haplotypes: 4 haplotypes of N. javanicus, 9 haplotypes N. coucang, and 2 haplotypes N.menagensis. Base on nucleotide variations at specific sites, it was formed haplotype differences among species.The average genetic distance between species showed that Kalimanatan population were closer to thepopulation of Sumatra (d= 0.042 ± 0.006) compared with Java population (d= 0.059 ± 0.009), whereas thegenetic distance within population showed the population of Java had low diversity (d= 0.002) with a meannucleotide differences 2.6. Thus Jawan slow loris (N. javanicus) should be conserved.Keywords: Nycticebus spp., molecular, COI, conservation, marker

PAKAN ALAMI DAN HABITAT KUKANG (NYCTICEBUS COUCANG) DAN . TARSIUS (TARSIUS BANCANUS) DI KAWASAN HUTAN PASIR PANJANG, KALIMANTAN TENGAH Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3279

ABSTRACTFeeds and habitat utilization of Slow Loris (Nycicebus coucang) and Tarsier (Tarsiusbnncartus) in Pasir Panjang Forest, Central Kalimantan. This research was conducted inthe production forest of Saka and Padang Babuluh in Pangkalan Bun. Study sites werelocated at 9-42 m above sea level. Data being collected were the type of feed, nest sites andhabitat utilization. During the observation, 44 plants species from 17 families were identifiedbeing consumed and nest site by the slow lories. Twenty one species from 17 families wereidentified being habitat by the tarsier. Nest were built on growing trees and litters were usedas bedding.Keywords : Nycticebus coucang, Tarsius bancanus, feeds, habitat, Pasir Panjang Forest

KERAGAMAN GENETIK PADA KUKANG (NYCTICEBUS COUCANG) BERDASARKAN PADA GEN 12S RRNA MITOKONDRIA Wirdateti, Wirdateti; Okayama, Toshinao; Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3478

ABSTRACTGenetic Diversity of Slow loris (Nycticebus coucang) based on Mitochondrial 12S rRNA gene. The research on genetic diversity of slow loris Nycticebus coucang (kukang) was carried out. The samples are 12 individuals from three locations (Sumedang and Jember in Java, and Lampung in Sumatera). Total DNA was extracted from blood and tissue. The mitochondrial 12S rRNA nucleotide sequences were determined to investigate genetic diversity of this species. This region was amplified by using L1091 and H 1478 primers by PCR. As a result of the analysis for 386 bp nucleotide sequence, five haplotypes were found, two from Java and three from Sumatra, respectively.Key words : Slow lories, Nycticebus coucang, genetic diversity, 12S rRNA mtDNA

Co-Authors A Kundarmasno, A ALEX SUMADIJAYA Bram Brahmantyo, Bram Dewi Puspitasari Didid Diapari, Didid G Semiadi GONO SEMIADI Hadi Dahrudin, Hadi Hellen Kurniati, Hellen Indriana, Eka Indriana, Eka Jamhari Jamhari M Mansur, M Ni Luh Phadmacanty, Ni Luh Nugraha, R Taufiq P Okayama, Toshinao Okayama, Toshinao Reksodihardjo, Andi Riyanto, Awal Soemarno, Suprayogo Tjakradidjaja, Anita S Tjakradidjaja, Anita S Wartika Rosa Farida Widyastuti, SK Yulianto Yulianto

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