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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA JURNAL BIOLOGI PAPUA Quagga: Jurnal Pendidikan dan Biologi

Dirk Y.P. Runtuboi

Universitas Cenderawasih

Author-ID : 5961179

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology

Published : 13 Documents Claim Missing Document

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Penentuan Vektor Malaria di Kabupaten Keerom, Papua Ign. Joko Suyono; Dirk Runtuboi; Aditya Krishar Karim; Sigit Raharjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 3 (2012): October 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i3.145

Malaria merupakan salah satu masalah kesehatan utama di beberapa wilayah didunia terutama di wilayah tropis seperti halnya di Papua. Malaria disebabkan oleh nyamuk Anopeles sebagai vektor malaria. Tujuan penelitian ini adalah menentukan dan mengidentifikasikan kemampuan nyamuk Anopheles menularkan penyakit malaria di Kabupaten Keerom. Status vektor ditentukan berdasarkan kapasitas vektorial atau pendeteksian kandungan sporozoit pada nyamuk Anopheles. Komposisi nyamuk yang ditemukan di lokasi penelitian adalah An. koliensis, An. farauti, An. punctulatus, An. subpictus dan An. brancroftii. An. subpictus dan An. brancroftii hanya ditemukan dalam jumlah yang kecil sehingga tidak dilakukan analisis kapasitas vektorial. Perhitungan kapasitas vektorial menunjukkan bahwa kapasitas vektorial An. koliensis berkisar dari 6% dan 17%, An. farauti antara 0,3% dan 3%, dan An. punctulatus berkisar antara 3% dan 5%. Deteksi kandungan sporozoit menggunakan Test VecTORTM menunjukkan tidak ditemukannya sporozoit pada nyamuk yang diteliti. Potensi nyamuk yang diduga merupakan vektor malaria di Kabupaten Keerom adalah An. koliensis, An. punctulatus dan An. farauti.Kata kunci: Vektor malaria, kapasitas vektorial, nyamuk Anopheles, sporozoit, VecTORTMTest

BUDIDAYA JAMUR TIRAM PUTIH (Pleurotus ostreatus) SEBAGAI PERCONTOHAN DAN UNIT USAHA BUDIDAYA JAMUR (UUBJ) DI UNIVERSITAS CENDERAWASIH verena agustini; Supeni Sufaati; Bonifasia Elita Bharanti; Dirk Yanes Runtuboi
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 1 (2018)
Publisher : Yogyakarta State University

Usaha jamur tiram putih (Pleurotus ostreatus) di Papua merupakan “bisnis baru” yang sangat menjanjikan. Unit Usaha Budidaya Jamur (UUBJ) yang merupakan implementasi program PPUPIK Jurusan Biologi FMIPA Universitas Cenderawasih (Uncen) telah merintis budidaya jamur tiram sejak tahun 2016. Jamur segar yang dihasilkan 15 kg per minggu dengan harga Rp. 70.000,-/kg. Selain sebagai profit, UUBJ juga menjadi sarana pembelajaran bisnis (wirausaha) bagi mahasiswa, alumni dan kelompok masyarakat. Solusi atau teknologi untuk menumbuhkan dan membentuk jiwa kewirausahaan adalah Entrepreunership Capacity Building (ECB) dan Participatory Action Learning System (PALS). Unit ini telah menghasilkan 5 orang entrepreuner baru di bidang usaha jamur tiram putih yang berasal dari mahasiswa dan alumni.

Uji Sensitivitas Neisseria gonorrhoeae terhadap Beberapa Antibiotik Pada Wanita Penjaja Seks (WPS) di Lokalisasi Tanjung Elmo Kabupaten Jayapura Hamid Hamid; Dirk Runtuboi; Lucky V. Waworuntu
Jurnal Biologi Papua Vol 6, No 2 (2014)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The purpose of this research is to study the sensitivity of quinolon antibiotic group against Neisseria gonorrhea. The bacteria which were found from endoservic vaginal from local sexual worker of Tanjung Elmo, Jayapura were isolated using neck duck sterile. This study was done on Health Laboratory of Jayapura from June to September 2013. The swab was planted on Thayer-Martin medium and incubated at 37 ºC for 2 x 24 hours. The grown colonies were sub-cultured to get the specific colonies. The bacterial identification is done by some rapid test including examination of grams, oxidation tests, biochemical tests andsensitivitytest. The result showed that there were six (6) specimen of 52 samples positively contained N. gonorrhea. The sensitivity test of quinolone group indicated that the levofloxacin, cefixime, ciprofoxacin, ofloxacin, azytromisin have the sensivity of 83.3%, 66.6%, 66.6%, 66.6% and 50% respectively.Key words: Neisseria gonorrhea, female commercial sex workers, antibiotics

Genetic Diversity of Merozoite Surface Protein 2 (MSP2) Plasmodium falciparum Clinical Isolate in Wamena General Hospital Dirk Y.P. Runtuboi; Rosye H.R. Tanjung; Yulius Sarungu; Meidy J. Imbiri; Irma A. Resmol; Ign. Joko Suyono
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The genetic diversity of typical clinical isolated Plasmodium falciparum in the malaria population varies greatly, especially at the location where malaria disease were recorded at high incidence rate. MSP2 is known as glycoprotein expressed on the surface of merozoites, which is an antigenic protein and has a potential to act as vaccine candidate for malaria. The MSP2 gene has two main allelic groups called FC27 and 3D7/IC. Block 3 from MSP2 gene is the most polymorphic to describe the diversity of parasite populations. The P. falciparum parasite population is often characterized by wide genetic diversity in areas of high transmission intensity. Therefore, the study on P. falciparum diversity is useful to describe the level of malaria transmission. The study of genetic diversity focused on clinical isolated species at Wamena General Hospital was aimed to determine the presence of the MSP2 gene, variety of MSP2 gene allele and the dominant frequency of the MSP2 gene allele. This research has been carried out from March 2018 to February 2019 using a cross sectional approach. The research sample was taken and prepared from Wamena Regional Hospital and followed by the analyzing of DNA isolation, PCR, electrophoresis of the research samples was done at the genetic science laboratory in Jakarta, Indonesia. The samples studied were patients who met the inclusion criteria, namely a single P. falciparum infection with an asexual parasite density >1000 parasites/µl or >3+ (1-10 P/Lp), and were agreed to become respondents by signing an informed consent. A total of 26 clinical isolates of P. falciparum were isolated with the MSP2 gene distribution on the FC27 allele with the highest as many as 25 samples (96.2%), 22 samples (84.6%) of the 3D7 / IC allele while the mixture of the two alleles was 22 samples (84.6%). From a total of 26 samples, there were samples with the male gender category counted for 77.3% and female 41%. The results of the identification of clinical isolated P. falciparum at Wamena Hospital with a total of 26 samples were found in productive age, between 15-34 years with a single allele (95.8%), while 23 cases and mix (both alleles 87.5%) about 21 cases, meanwhile in cases of before-productive age, in which ages were 12 and 14 years of age with a single allele 100% (FC27) 2 cases and 50% (3D7/IC) found to be 1 case, The mixture of the two alleles is 50% was only 1 case and there was no sample at non-productive age observed. Key words: Malaria; MSP-2; P. falciparum; Wamena

Uji Resistensi Antibiotik dan Deteksi Gen Plasmid IncHI1 Salmonella typhi Isolat Jayapura Rini S. Kelanit; Dirk Y.P. Runtuboi; Tri Gunaedi
Jurnal Biologi Papua Vol 8, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

The research on antibiotic resistance test and detection of gene Plasmid IncHI1 1 Salmonella typhi isolates of Jayapura have been conducted in January to August 2015. The purpose of this study is to determine antibiotic resistance patterns and detect the presence ofS. typhi IncHI1 plasmid genes in Jayapura.The method of study was the laboratory analytic where ninety two samples were culture tested and S. typhi identified was tested using disc diffusion method of Kirby Bouer to see any resistance toward 18 antibiotics. Plasmids of S. typhi then were extracted using method of Kado-Liu. To ensure the presence of S. typhi DNA, the testof flagellin gen detection had been done using Nested PCR followed by gene Inchi1 S. typhi detection. The results showed that among 8 isolates of S. typhi, there has been a Multi Drug Resistant (MDR) of 7 samples (87.5%) with varying resistance patterns where the most resistant antibiotics are Amoxicillin 100% (8 isolates), Cefazolin 75% (6 isolates), Ampicillin 75% (6 isolates), Trimethoprim–Sulfamethoksazol 62.5% (5 isolates), Amikacin 62.5% (5 isolates), Gentamicin 50% (4 isolates) and Ampisillin–Sulbactam 50% (4 isolates). The most sensitive antibiotics is Meropenem is 87.5% (7 isolates). PCR test results showed that there were no genes of IncHI1 S. typhi in Jayapura.Key words: Salmonella typhi, gen IncHI1, antibiotic resistance, Jayapura.

Studi Kesesuaian Lahan Budidaya Rumput Laut Berdasarkan Parameter Biofisik Perairan di Yensawai Distrik Batanta Utara Kabupaten Raja Ampat Dirk Runtuboi; Yunus P. Paulungan; Tri Gunaedi
Jurnal Biologi Papua Vol 6, No 1 (2014)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

One of the potential areas for developing mariculture in Raja Ampat Regency is Yensawai. This research aim to determine biophysical parameter for seaweed culture. The parameters were proped in to four classes; high suitable, suitble, currently not suitable and permanently not suitable. The result showed that the cultivation area size were 14 hectares, where Ramon 1 and Ramon 2 Stations were categorized as currently not suitable, while Warkabas and Metabai stations were grouped into suitable for seawed cultivation.Key words: analysis of suitability, seaweed cultivation, Yensawai

Karakterisasi Bakteri Bacillus thuringiensis asal Hutan Lindung Kampus Uncen Jayapura, serta Deteksi Toksisitasnya terhadap Larva Nyamuk Anopheles Daniel Lantang; Dirk Y.P. Runtuboi
Jurnal Biologi Papua Vol 4, No 1 (2012)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Study of isolate characterization of Bacillus thuringiensis Berliner collected from soil was done at UNCEN Campus. The aims of the study were to explore the local isolate of B. thuringiensis and to study the toxicity of the isolates on larvae of Anopheles as a vector of malaria and filariasis deseases in Papua. Data was analized by Hadioetomo & Rusmana method (1977). The results showed that 383 colonies are relatively closed to B. thuringiensis whereas 290 of them are indicated as local isolates of B. thuringiensis. Based on toxicity detection on larvae of Anopheles found that 19 isolates have toxicities levels 50% or more. Among the 19 isolates 3 of them has 80%, 85% and 90% toxicity levels separately.Key words: Characterization, local isolate, B. thuringiensis, toxicity, and soil.

Bakteri Penyebab Infeksi Nosokomial Pada Alat Kesehatan dan Udara di Ruang Unit Gawat Darurat RSUD Abepura, Kota Jayapura Herlando Sinaga; Dirk Y.P. Runtuboi; Lisye I. Zebua
Jurnal Biologi Papua Vol 6, No 2 (2014)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Nosocomial infection is a health care problem in hospital worldwide. Problem, that appeared may aggravate existing disease, even cause death. Nosocomial infection can be defined as an infection acquired or occurring in hospital. The aimed of study was to determinespecies of bacteria causing nosocomial infection on medical equipment and air sampling in Accident and Emergency Room at Jayapura Hospital. This research was conducted at the Regional Health Laboratory (LABKESDA) Jayapura. The result showed that there were 13 species of bacteria found and there were 5 species of Staphylococcus cohnii, Klebsiella spp., Serratia marcescens, Staphylococcus haemolyticus, and Streptococcus spp.in the medical equipments at Emergency Room. Moreover, in air sampling of emergency room was founded 13 species of bacteria namely Staphylococcus haemolyticus (14%), Klebsiella spp (12%), Ser. marcescens (12%), E. Coli (12%), Sta. aureus (11%), Streptococcus spp (11%), Citrobacter sp (8%), Enterobacter cloacae (8%), Sta. cohnii (4%), Pneumococcus 2%), Proteus spp. (2%), Sta. epidermidis (2%), and Sta. warneri (2%).Key words: Nosocomial infections, health equipments, air, hospital.

Aktivitas Antibakteri Ekstrak Etanol Daun Gatal (Laportea aestuans (L.) Chew) Eva S. Simaremare; Agustina Ruban; Dirk Y.P. Runtuboi
Jurnal Biologi Papua Vol 9, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Daun gatal (Laportea aestuans) is an indigenous plant of Papua hich has been widely used for pain relief as traditional medication in the local community. The leaves were just picked then treated to cure for painful body. This treatment are giving itchy sense as an indication that medication is working on the body as local people assumed. The aim of the study was to determine the activity of antibacteria of daun gatal against Escherichia coli, Staphylococcus aureus and Salmonella typhi. Samples of daun gatal were collected from Biak Numfor Papua. The methods are started with filtering of daun gatal using 100 mash and extracted with ethanol. Extract was made in 250, 500, 750, 1000 ppm, with chloramphenicol as a control positive, while the test of antibacterial activity was done by disc diffusion method. The results showed that daun gatal has antibacterial activity against E. coli, S. aureus and S. typhi. Daun gatal extract (L. aestuans) is effective in inhibiting the growth of bacterias with zone inhibition of 1000 ppm extracts which were 8.55 mm (E. coli), 9.02 mm (S. typhi), dan 9.37 mm (S. aureus). Key words: Antibacteria, E. coli, L. aestuans, S. aureus and S. typhi.

Isolasi dan Identifikasi Bakteri Termofilik dari Sumber Air Panas di Moso Distrik Muara Tami Kota Jayapura Provinsi Papua Dirk Y.P. Runtuboi; Tri Gunaedi; Maria Simonapendi; Nadya N.L. Pakpahan
Jurnal Biologi Papua Vol 10, No 2 (2018)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Thermophilic bacteria are bacteria that are able to survive in environmental conditions with high temperatures. Thermophilic bacteria are one of the important sources of thermostable enzymes that can be isolated from geothermal environments such as hot springs with temperatures ranging from 50–80 oC. Aims of the study to isolate and identify local isolates of thermophilic bacteria from hot springs in Moso Muara Tami District, Papua. The results showed that 7 isolates (A1, A2, A3, A4, B1, B2, and B3) that were isolated and identified based on phenotypic characters were included in the genus Bacillus. Key words: Thermophilic, characterization, isolation, identification, phenotypic.

Co-Authors Aditya Krishar Karim Agustina Ruban Alum Turnip Bonifasia Elita Bharanti Daniel Lantang Daniel Lantang Eva S. Simaremare Hamid Hamid Herlando Sinaga Ign. Joko Suyono Ign. Joko Suyono Irma A. Resmol Lisye I. Zebua Lucky V. Waworuntu Maria Simonapendi Marthen L.R. Bartholomeus Meidy J. Imbiri Nadya N.L. Pakpahan Rahayu Samalo Rini S. Kelanit Rosye H.R. Tanjung Sigit Raharjo Supeni Sufaati Tri Gunaedi Tri Gunaedi Tri Gunaedi Tri Gunaedi Verena Agustini Yulius Sarungu Yunus P. Paulungan

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