Article Per Year (5 Year)
p-Index From 2019 - 2024
1.704
P-Index
This Author published in this journals
All Journal ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Material Kedokteran Gigi Odonto dental journal JPMP
Fitria Rahmitasari
Department Of Dental Materials, Faculty Of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
Particle size analysis of yellowfin tuna (Thunnus albacares) skin collagen powder using papain-soluble collagen method with varying NaCl concentrations: an experimental laboratory Destya Arizha Fatryana; Fitria Rahmitasari; Chaterina Diyah Nanik K.; Moh. Basroni Rizal; Saka Winias
Padjadjaran Journal of Dentistry Vol 36, No 2 (2024): July 2024
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no2.54588

Abstract

ABSTRACTIntroduction: The skin of yellowfin tuna (Thunnus albacares) contains high protein, which could potentially be a halal collagen product. Collagen extraction using the papain-soluble collagen method has the advantage of producing a higher collagen yield compared to the acid method. Particle size, one of the physical properties of collagen, plays a crucial role in its efficacy in dentistry. This study aims to analyze the particle size of collagen powder synthesized   from Thunnus albacares skin using the papain-soluble collagen method, with varying concentrations of NaCl. Methods: Type of research is an experimental laboratory. Thunnus albacares skin was synthesized by chopping, cleaning, and soaking in a 0.1 M NaOH solution. The extraction process used the enzyme papain and 0.5 M of acetic acid. Samples were divided into four groups, each with different concentrations of NaCL: 0, no NaCL;, 0.9 M NaCl, 1.3 M NaCl, and 1.7 M NaCl. After centrifugation, the samples were freeze-dried. The particle size of collagen powder was measured using a Particle Size Analyzer test tool. The data collected was then analyzed using the Mann-Whitney test. Results: Particle size distributions are as follows: K group (3.36 nm), P1 (1.842 nm), P2 (3.36 nm), and P3 (10.12 nm). There is a significant difference in groups K-P1 and P3, P1-P2 and P3, and P2-P3 (p<0.05). However, there is no significant difference in groups K-P2 (p>0.05). Conclusion: Particle size of this research produced nano-sized collagen powder, with the lowest particle size observed in the 0.9 M NaCl group, measuring at 1.842 nm. The particle size increased in the group without NaCl and in the 1.3 M NaCl group at 3.36 nm, and reached the highest value in the 1.7 M NaCl group at 10.12 nm. 
Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Kartika Zefanya; Fitria Rahmitasari; Widaningsih Widaningsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
Co-Authors Adelia Tinisia Andriani, Dwi Annisa Listya Paramita Ari Rosita Irmawati Arifzan Razak Caecilia Indarti Chaterina Diyah Nanik K. Destya Arizha Fatryana Dwi Setianingtyas Elly Munadziroh Ester Arijani Rachmat Grace Caroline Setiawan I.G.A.M Oka Lestari Ivan Tantra Kartika Zefanya Mardiyanto Riski Hartono Moh. Basroni Rizal Muhammad Galang Adhinata Abdul Rahim Nina Nilawati Novendy Yoyada Puguh Bayu Prabowo Puguh Bayu Prabowo Retno Pudji Rahayu Saka Winias Shintya Rizki Ayu A Sularsih Sularsih Sularsih Sularsih Twi Agnita Cevanti Widaningsih Widaningsih Widyasri Prananingrum Widyasri Prananingrum Widyasri Prananingrum Wisnu Setyari J Yulie Emilda