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Beatrix Doodoh
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Agriculture, Biological Sciences & Forestry Environmental Science

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PENGARUH ZAT PENGATUR TUMBUH Benzil Amino Purin (BAP) TERHADAP INDUKSI DAN MULTIPLIKASI TUNAS BROKOLI Brassica oleraceae L. var. italica Plenck Elvis Sagai; Beatrix Doodoh; Deanne Kojoh
COCOS Vol. 7 No. 6 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v7i6.13885

Abstract

ABSTRACTThis study aims to find out about the effect of plant growth regulators BAP on induction and multiplication shoots of broccoli Brassica oleracae L. var. italica Plenck. This study has been carried out at the Laboratory of Biotechnology Faculty of Agriculture for 3 months starting from April to July 2016. This study used a completely randomized design (CRD), which consists of a single factor, namely the concentration of BAP with 6 treatments and 4 replications. Shoots of broccoli isolated from sterile sprouts and maintained on shoots induction medium consisting of Murashige and Skoog basic medium. Benzylaminopurine (BAP) with a concentration of 1 ppm to 5 ppm is added to the media for inducing and multiplication shoots of broccoli. Results of the study showed that with a concentration of 1 ppm BAP,2 ppm and 5 ppm induce shoots faster than other treatments while the number of buds Award BAP concentration of 1 ppm, 4 ppm and 5 ppm produced the highest number of shoots among other treatments.Keywords: Shoots of broccoli, Benzilaminopurin, induction and multiplication
PEMBERIAN PUPUK ORGANIK CAIR TERHADAP PERTUMBUHAN DAN HASIL PADA TANAMAN MENTIMUN (Cucumis sativus L.) Paul Worek; Beatrix Doodoh; Sofia Demmassabu
COCOS Vol. 10 No. 7 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i5.25615

Abstract

ABSTRACT This study aims to determine the effect of concentration of liquid organic fertilizer (POC) on growth and yield on cucumber plants. This research was carried out in the village of Kolongan Tetempangan, Kalawat District, North Minahasa Regency for 2 months from July to August 2018. This research was conducted using the Completely Randomized Design method, with five treatments and four replications, namely the concentration of liquid organic fertilizer: 0 ml POC /2l water (P0), 2 ml POC /2l water (P1), 4 ml POC /2l water (P2), 6 ml POC /2l water (P3) and 8 ml POC /2l water (P4). Observations were made on each plant with observational variables, namely plant height, number of leaves, flowering time, number of flowers, number of fruits, fruit length, fruit diameter and fruit weight. The results showed that the administration of liquid organic fertilizer G Tonik has not given a significant effect on the growth of cucumber plants, but it has an effect on the production of cucumber plants in this case the fruit weight. Keywords : concentration, liquid organic fertilizer (POC), growth, hybrid cucumber
INDUKSI KALUS PADA AREN (Arenga pinnata Merr.) SECARA IN VITRO MENGGUNAKAN 2,4-D Jefri Paseno; Euis F. S. Pangemanan; Beatrix Doodoh
COCOS Vol. 12 No. 4 (2020): EDISI OKTOBER-DESEMBER 2020
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i1.31820

Abstract

ABSTRACT CALLUS INDUCTION ON AREN (Arenga pinnata Merr.) IN VITRO USING 2,4-D This study aims to obtain the optimal concentration of ZPT (growth regulator) 2,4-D for in vitro induction of palm callus.The research was conducted in January - May 2020 at the Laboratory of the Manado Palm Plant Research Institute.This study used MS media (Murashige and Skoog) and 5 concentrations (treatment) of 2,4-D, each treatment was repeated 5 times so that the number of experimental units was 25 bottles of culture, namely the initial concentration using 2,4-D 2 mg /l, 3 mg / l, 4 mg / l, 5 mg / l, and 6 mg / l, while for the concentration of 2,4-D after sub-culture, namely, 6 mg / l, 7 mg / l, 8 mg /l, 9 mg / l, and 10 mg / l.The results showed that the treatment of 2,4-D at a dose of 6 mg / l and 10 mg / l could induce callus on sugar palm. Keywords : Arenga pinnata Merr., Plant Tissue Isolation Method, Callus Induction, 2,4-D
PENGGUNAAN ZAT PENGATUR TUMBUH SITOKININ DAN EKSTRAK BAHAN ORGANIK TERHADAP PERTUMBUHAN ANGGREK DENDROBIUM SECARA In-Vitro Bryan Mokoginta; Beatrix Doodoh; Doortje. M. F. Sumampow
COCOS Vol. 12 No. 4 (2020): EDISI OKTOBER-DESEMBER 2020
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v2i2.32446

Abstract

ABSTRAKDendrobium orchids are one of the favorite orchid genera for orchid lovers. This is becausethis orchid is able to adapt to various growing environmental conditions. The growth media in tissueculture has a very big influence on the growth and development of the explants and the seeds itproduces. Cytokinins that play a role in stimulating cell division and inducing shoot formation. Someextracts of organic materials that are often used in plant tissue culture are extracts of tomatoes, corn,bananas and coconut water. This study aims to determine the best results of using cytokinins andextracts of organic matter and coconut water for in-vitro growth of Dendrobium orchids. This researchis a library research, which is a series of studies that are concerned with the method of collectinglibrary data, or research where the object of research is explored through various library information(books, encyclopedias, scientific journals and documents). The data used in this research is secondarydata. The data collection method used in this research is the documentation method. . The data analysisused in this study was annotated bibliography analysis.Based on research conducted in the form of literature searches, it can be concluded that byusing growth regulators cytokinins (in this case BAP) and extracts of organic matter, banana extract,tomato extract, corn extract and coconut water, it turns out that the best results are obtained with theuse of growth regulators Cytokinins ( namely BAP) 3 ppm with tomato extract organic matter.Kata Kunci: Zat Pengatur Tumbuh, Bahan Organik, Anggrek Dendrobium
Effect of Preservative Solution Composition and Stalk Cut Angle in Maintaining the Shelf Life of Cut Flowers Chrysanthemum (Chrysanthemum morifolium R.) var. Kulo Windi Lanni Timpalan; Beatrix Doodoh; Maria G. M. Polii; Jeanne M. Paulus; Meity R. Rantung; Annatje E. B. Inkiriwang
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.49126

Abstract

This study aims to examine the interaction between the composition of the preservative solution and the cut angle of the flower stalk in maintaining the shelf life of cut chrysanthemum flowers and to examine the effect of the composition of the preservative solution in maintaining the shelf life of cut chrysanthemum flowers and to examine the effect of the angle of cut flower stalks in maintaining the shelf life of cut chrysanthemum flowers. This research was conducted from January 2023 to February 2023 at the Genetics and Plant Breeding Laboratory. This study used a completely randomized design (CRD) factorial pattern with 2 factors, namely Factor A consisting of 3 levels with treatment A0: Water, A1: Sugar 25 g/L + Citric Acid 0.15 g/L + Bleach 1 mL /L, and A2: 25 g/L Sugar + 0.4 mL/L Vinegar + 1 mL/L Bleach and Factor B consists of 3 levels with treatment B1: Flat Cut (0Ëš), B2: Slanted Cut (30 Ëš), and B3: Bevel Cut (45Ëš). So that there were 9 treatment combinations and each treatment was repeated 3 times, so 27 experimental units were obtained. Each experimental unit has 2 samples, so that the total number of experimental units is 54 samples. Research data were analyzed using analysis of variance 2 factor ANOVA method with repetition and if it has an effect then it will be continued with the BNT test at the 5% level. The results showed that there was no interaction between the composition of the preservative solution and the cut angle of the flower stalks for all the observed variables. The composition of the preservative solution treatment A2: Sugar 25 g/L + Vinegar 0.4 mL/L + Bleach 1 mL/L had a single significant effect on maintaining the shelf life of cut flowers of chrysanthemum var. Kulo for 16.28 days. The cutting angle of the flower stalk had no significant effect on maintaining the shelf life of the cut chrysanthemum var. Kulo. Keywords: Preservative Solution, Cut Flowers, Chrysanthemum Abstrak Penelitian ini bertujuan untuk mengkaji interaksi antara komposisi larutan pengawet dengan sudut potong tangkai bunga dalam mempertahankan masa pajang bunga potong krisan dan mengkaji pengaruh komposisi larutan pengawet dalam mempertahankan masa pajang bunga potong krisan serta mengkaji pengaruh sudut potong tangkai bunga dalam mempertahankan masa pajang bunga potong krisan. Penelitian ini dilaksanakan pada bulan Januari 2023 sampai Februari 2023 di Laboratorium Genetika dan Pemuliaan Tanaman. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor, yaitu Faktor A terdiri dari 3 taraf dengan perlakuan A0: Air, A1: Gula Pasir 25 g/L + Asam Sitrat 0,15 g/L + Pemutih 1 mL/L, dan A2: Gula Pasir 25 g/L + Cuka 0,4 mL/L + Pemutih 1 mL/L dan Faktor B terdiri dari 3 taraf dengan perlakuan B1: Potongan Datar (0Ëš), B2: Potongan Miring (30Ëš), dan B3: Potongan Miring (45Ëš). Sehingga terdapat 9 kombinasi perlakuan dan setiap perlakuan diulang sebanyak 3 kali, maka didapatkan 27 satuan percobaan. Setiap satuan percobaan terdapat 2 sampel, sehingga total keseluruhan satuan percobaan terdapat 54 sampel. Data penelitian dianalisis dengan menggunakan analisis ragam metode ANOVA 2 Faktor dengan pengulangan dan apabila berpengaruh maka akan dilanjutkan dengan uji BNT pada taraf 5%. Hasil penelitian menunjukkan tidak terdapat interaksi antara komposisi larutan pengawet dengan sudut potong tangkai bunga terhadap semua variabel pengamatan. Komposisi larutan pengawet perlakuan A2: Gula Pasir 25 g/L + Cuka 0,4 mL/L + Pemutih 1 mL/L berpengaruh nyata secara tunggal dalam mempertahankan masa pajang bunga potong krisan var. Kulo selama 16,28 hari. Sudut potong tangkai bunga tidak berpengaruh nyata dalam mempertahankan masa pajang bunga potong krisan var. Kulo. Kata Kunci: Larutan Pengawet, Bunga Potong, Krisan
Utilization of Water Hyacinth (Eichornia crassipes) as Liquid Organic Fertilizer (POC) on Chinese Cabbage (Brassica rapa var. parachinensis). Frilly Kawet; Jeany Sh. Mandang; Beatrix Doodoh; Stanley A. F. Walingkas; Rinny Mamarimbing; Pemmy Tumewu
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.49175

Abstract

This study aims to examine the utilization of water hyacinth (POC) on the growth and yield of Chinese cabbage and to determine the best concentration to improve the growth and yield of Chinese cabbage. The research was conducted in Tara-tara Village, Tomohon District, Tomohon City. The research design used a completely randomized design (CRD) consisting of 5 treatments and 4 replications, making a total of 20 plots: 0% (P0), 10% (P1), 20% (P2), 30% (P3), and 40% (P4). The observed variables were plant height, number of leaves, and wet weight of the plant. The results showed that water hyacinth POC had an effect on plant height in the fourth week after transplanting. It also had a significant effect on the wet weight of Chinese cabbage after harvest. The best concentration of water hyacinth LOF was achieved at a concentration of 40%. Keywords: Chinese cabbage, Liquid Organic Fertilizer, Concentration. Keywords; Growing medium, Roasted Rice Husk, Mustard plants Abstrak Penelitian ini bertujuan untuk mengkaji pemanfaatan pupuk organic cair (POC) eceng gondok terhadap pertumbuhan dan hasil tanaman sawi hijau dan mendapatkan konsentrasi terbaik untuk meningkatkan pertumbuhan dan hasil tanaman sawi hijau. Penelitian ini dilaksanakan di Desa Tara-tara, Kecamatan Tomohon, Kota Tomohon. Rancangan penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 4 ulangan sehingga berjumlah 20 petak yaitu perlakuan 0 (P0), 10% (P1), 20% (P2), 30% (P3), 40% (P4). Variabel yang diamati : tinggi tanaman, jumlah daun, dan berat basah tanaman. Hasil penelitian menunjukkan bahwa POC eceng gondok berpengaruh terhadap tinggi tanaman pada minggu keempat setelah pindah tanam. Juga berpengaruh nyata pada berat basah tanaman sawi hijau setelah panen. Konsentrasi POC eceng gondok terbaik dicapai pada konsentrasi 40%. Kata Kunci: Sawi Hijau, Pupuk Organik Cair, Konsentrasi
The Effect of Addition of Liquid Organic Fertilizer (POC) to Tofu Liquid Waste on POC Kirinyuh to Increase the Number of Tillers of Rice (Oryza sativa L.) Antje Grace Tulungen; Maria G. M. Polii; Jelie Viekson Porong; Stanley A. F. Walingkas; Beatrix Doodoh; Jeane S. M. Raintung; Stella M. Th. Tulung; Rinny Mamarimbing; Tommy Djoice Sondakh
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.49395

Abstract

Tofu liquid organic fertilizer (POC limbah cair tahu)  and Kirinyuh liquid organic fertilizer (POC Kirinyuh) will complement each other's nutrient needs and increase rice yields. The study aims to 1) study the effect of adding POC limbah cair tahu to POC Kirinyuh to increase the number of rice plant tillers, 2) obtain the dose of POC limbah cair tahu and POC Kirinyuh in increasing the number of productive tillers of rice plants. The study used a factorial pattern Complete Randomized Design.  The treatment consists of 3 treatments repeated 5 times.  The treatment is: A = 50 ml POC limbah cair tahu/liter water, B = 50 ml POC Kirinyuh/ 1 liter water+50 ml POC limbah cair tahu /liter water, C = 100 ml POC Kirinyuh/ 1 liter water+50 ml POC limbah cair tahu /liter water. The variables observed were the total number of tillers and the number of productive tillers.  The data were analyzed using ANOVA and if there was an effect of treatment, it was continued with the Smallest Real Difference Test (BNT) at the test level of 5%. The results showed that POC limbah cair tahu added to  POC Kirinyuh increased the number of rice plant tillers.  The concentration of 50 ml of POC limbah cair tahu / 1 liter of water added to 100 ml of POC Kirinyuh / 1 liter of water gives the total number of tillers and the highest number of productive tillers at 65.50 and 45.25 per pot. Keywords: number of rice plant tillers, POC tofu liquid waste, POC Kirinyuh Abstrak POC limbah cair  tahu dan POC Kirinyuh akan saling melengkapi kebutuhan hara dan meningkatkan hasil tanaman padi. Penelitian bertujuan untuk 1) mempelajari pengaruh  penambahan POC limbah cair tahu pada POC Kirinyuh untuk meningkatkan jumlah anakan tanaman padi,  2)  Memperoleh dosis POC limbah cair tahu dan POC Kirinyuh dalam meningkatkan jumlah anakan produktif  tanaman padi. Penelitian menggunakan Rancangan Acak Lengkap pola faktorial.  Perlakuan terdiri dari 3 perlakuan yang diulang sebanyak 5 kali.  Perlakuan tersebut adalah: A = 50 ml  POC limbah cair tahu/liter air, B =  50 ml  POC Kirinyuh/ 1 liter air+50 ml  POC limbah cair  tahu/liter air, C =  100 ml  POC kirinyuh/ 1 liter air+50 ml  POC limbah cair tahu/liter air. Variabel yang diamati adalah jumlah anakan total dan jumlah anakan produktif.  Data  dianalisis menggunakan  Anova dan jika terdapat pengaruh perlakuan dilanjutkan dengan Uji Beda Nyata Terkecil (BNT) pada taraf uji 5 %. Hasil penelitian menunjukan bahwa POC limbah cair tahu yang ditambahkan pada  POC Kirinyuh  meningkatkan jumlah anakan tanaman padi.  Konsentrasi 50 ml  POC limbah cair tahu/1 liter air ditambahkan pada  100 ml  POC kirinyuh/1 liter air memberikan jumlah anakan total dan jumlah anakan produktif  paling banyak  yaitu 65,50 dan 45,25 per pot. Kata kunci : jumlah anakan padi, POC limbah cair tahu ,  POC Kirinyuh
Co-Authors Annatje E. B. Inkiriwang Antje Grace Tulungen Bryan Mokoginta Deanne Kojoh Doortje. M. F. Sumampow Elvis Sagai Euis F. S. Pangemanan Frilly Kawet Jeane S. M. Raintung Jeanne M. Paulus Jeany Sh. Mandang Jefri Paseno Jelie Viekson Porong Maria G. M. Polii Meity R. Rantung Paul Worek Pemmy Tumewu Rinny Mamarimbing Rinny Mamarimbing Sofia Demmassabu Stanley A. F. Walingkas Stanley A. F. Walingkas Stella M. Th. Tulung Tommy Djoice Sondakh Windi Lanni Timpalan