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Correlation between malondialdehyde level and FOXO3 and CASP3 mRNA expression changed in early-onset preeclampsia placenta Ni Made Wiasty Sukanty; Febriana Catur Iswanti; Syarifah Dewi; Muhammad Faruqi; Alyssa Shafa Andiana; Ani Retno Prijanti
Acta Biochimica Indonesiana Vol. 4 No. 2 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.61

Background: Preeclampsia is one of the factors causing the high maternal mortality rate. The risk of morbidity and mortality is higher in Early Onset Preeclampsia (EOPE). Failure of spiral artery remodeling can cause oxidative stress that can inhibit placental development and increase trophoblast apoptosis. Objective: This study aims to analyze the oxidative stress and apoptosis of EOPE placentas. Methods: This study is an observational study with a cross-sectional design. A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. There was a strong positive correlation between FOXO3 and CASP3 mRNA expression in the normal (p= 0.0001; r = 0.938) and EOPE groups (p = 0.0001; r = 0.855). There was no correlation between MDA concentration to FOXO3 (p = 0.124; r = 0.282) and CASP3 (p = 0.569; r = 0.106) mRNA expression in normal placenta. There was positive correlation between MDA concentration to FOXO3 (p = 0.016; r = 0.429) and CASP3 mRNA expression in EOPE placenta (p = 0.028; r = 0.395). Conclusion: These results indicate that cell integrity is still maintained through the autophagy process and the level of apoptosis in the EOPE placenta is regulated by ROS through FOXO3.

The role and regulation of FOXO1 in carbohydrate metabolism and its targeting in metabolic diseases Ani Retno Prijanti; Ni Made Wiasty Sukanty
Indonesian Archives and Biomedical Sciences Vol 1 No 2 (2021): Indonesian Archives of Biomedical Research (InABR). 1(2): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Carbohydrate is the first energy source used in metabolic processes. Failure in carbohydrate metabolism can cause metabolic diseases, and many studies are focused on its therapy. Until now, there is no specific therapy approved. Despite that, one of the main alternatives is regarding the involvement of FOXO1 protein in metabolic disease progress. As a transcription factor for gluconeogenesis genes, FOXO1 can increase blood glucose levels. It also involves various signaling pathways in carbohydrate metabolisms such as PI3K/Akt and PKA in which many proteins act as FOXO1 regulators through posttranslational modification. The vital role of FOXO1 in carbohydrate metabolism provides an opportunity to make FOXO1 the main target of metabolic disease therapy. Various proteins and natural compounds can either directly or indirectly regulate FOXO1 activity. It can be an option to be used to control blood glucose levels by targeting FOXO1

Analisis Kesenjangan Antara Harapan Dengan Kenyataan Yang Dirasakan Peserta Posbindu Widani Darma Isasih; Junendri Ardian; Wanda Januar Astawan; Novia Zuriatun Solehah; Ni Made Wiasty Sukanty
Nutriology : Jurnal Pangan,Gizi,Kesehatan Vol 3 No 2 (2022): Oktober 2022
Publisher : Program Studi Gizi, Universitas Bumigora

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30812/nutriology.v3i2.2460

Rasio pemanfaatan peserta POSBINDU FKM UNAIR yang masih rendah dan hasil penelitain berupa gap menunjukkan adanya ketidak puasan yang dirasakan peserta. Gap berdasarkan perhitungan persentase harapan (H) dikurangi dengan persentase kenyataan yang dirasakan oleh peserta (K). Jika H – K > 0 (nilai postif) maka dikatakan hal tersebut bermasalah. Jika nilai GAP diperoleh = atau > 20% maka dapat dikatakan bahwa hal tersebut merupakan hal yang penting untuk diperhatikan sesuai dengan analisis pareto, karena peresentasi = atau > 20% ketidak puasan dapat menjadi pemicu timbulnya masalah. Hasil penelitian menunjukkan persentase harapan lebih tinggi di bandingkan persentase kenyataan yang lemudian menyebabkan terjadinya rasa ketidak puasan pada peserta. Jika kenyataan sesuai harapan, konsumen puas. Jika kenyataan melebihi harapan, konsumen sangat puas. Tetapi jika kenyataan tidak sesuai dengan harapan, maka konsumen akan kecewa. Semakin besar kesenjangan (gap) antara harapan dan kenyataan, semaakin besar kekecewaan konsumen.

Edukasi PHBS dalam Upaya Peningkatan Derajat Kesehatan Masyarakat di Tatanan Rumah Tangga Ni Made Wiasty Sukanty; Lina Yunita; Baiq Fitria Rahmiati; Wanda Januar Astawan; Defel Septian
ADMA : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 3 No 2 (2023): ADMA: Jurnal Pengabdian dan Pemberdayaan Masyarakat
Publisher : LPPM Universitas Bumigora

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30812/adma.v3i2.2498

Clean and healthy living behavior (PHBS) in the household is one of the government's programs to improve people's welfare. PHBS indicators in homes include using clean water, washing hands with soap and clean water, and using healthy latrines. Lack of knowledge and public awareness of the importance of PHBS and inadequate health support facilities and infrastructure can be a factor causing high rates of morbidity and mortality due to a disease. One of the means of supporting public health is the availability of water sources. The decrease in river water discharge in Lembah Sari Village can affect sanitation related to PHBS in the community. We have conducted PHBS counseling activities in Lembah Sari Village using PowerPoint media, posters, and teaching aids. It was an effort to help people know, want, and practice PHBS. After receiving counseling, the community became more aware of PHBS. The community seemed enthusiastic and participated in practicing washing hands with soap as an indicator of PHBS. This activity is expected to be applied by the community daily.

Ekstraksi Alginat dari Rumput Laut Coklat (Phaeophyceae) dan Pemanfaatannya Sebagai Pengemulsi (Emulsifier) pada Produk Pangan Farida Ariani; Lina Yunita; Novianti Tysmala Dewi; Ni Made Wiasty Sukanty; Widani Darma Isasih
Nutriology : Jurnal Pangan,Gizi,Kesehatan Vol 4 No 1 (2023): April 2023
Publisher : Program Studi Gizi, Universitas Bumigora

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30812/nutriology.v4i1.2859

Alginate is used as an emulsifier in food production. Its non-toxic, biocompatible, biodegradable, and hydrophilic properties are very promising for industrial use. Alginate can be obtained from brown seaweed because the alginate content in brown seaweed reaches 40% of its dry weight. The alginate extraction process can be carried out in 5 main stages, 1) extraction process using mineral acids, 2) filtering or centrifugation process, 3) re-soaking of the residue using an alkaline solution, 4) precipitation process, and 5) purification. Emulsifiers are molecules that adsorb on the surface of the droplets formed during the homogenization process and form a protective membrane that keeps the droplets from agglomerating. The use of alginate as an emulsifier in food has been carried out by several researchers. The results indicate that alginate substitution in food products has a significant effect on emulsion stability, where the stability of emulsions using alginate is higher than that of emulsions without alginate substitution. In addition, there was a positive interaction between alginate-substituted food products and food products without alginate substitution (control) on storage time. This research aims to provide an overview of the method or process of extracting alginate from brown seaweed (Phaeophyceae) and utilizing alginate as an emulsifier for food.

STATUS GIZI, KEBIASAAN SARAPAN PAGI DAN KELELAHAN KERJA PADA PEKERJA KAPAL CEPAT (FAST FERRY) PT. BALI EKAJAYA LINA YUNITA; NI MADE WIASTY SUKANTY; NOVIANTI TYSMALA DEWI; FARIDA ARIANI
GANEC SWARA Vol 17, No 2 (2023): Juni 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i2.478

Fatigue is mental or physical fatigue that makes it difficult for a person to work comfortably and effectively. One of the factors contributing to reducing the level of work fatigue is by fulfilling worker nutrition so as to improve health. The risks of burnout include work-related stress, work-related illnesses and work-related accidents. This study aims to analyze the relationship between nutritional status, breakfast habits and work fatigue among employees of fast ferry shipping companies. This type of research used an analytic survey with a cross sectional study approach. This research was carried out at Sengig Port, West Lombok Regency, Nusa Tenggara. The sample of this research is the entire crew of the three fast boat fleets with a total of 41 samples. The research variables are nutritional status, breakfast habits and work fatigue. Nutritional status data is obtained by directly measuring body weight and height. Breakfast data was obtained by interviewing using a 24-hour recall form. Fatigue data was obtained using a fatigue questionnaire. The results showed that most of the workers used to have breakfast, almost 68.3%. Respondents who had normal nutritional status were 51.2% and workers who experienced mild fatigue were 58.5%. Statistical test results for breakfast with nutritional status obtained p = 0.01, statistical tests for breakfast with fatigue obtained p = 0.23, and statistical test results for nutritional status with fatigue obtained p = 0.37. there is a relationship between breakfast, nutritional status and fatigue in workers but there is a relationship between breakfast habits and nutritional status in fast boat company employees at Senggigi port

PTEN: The Potential Therapeutic Target of Diabetes Mellitus Ni Made Wiasty Sukanty; I Putu Bayu Agus Saputra; Lina Yunita
Poltanesa Vol 24 No 1 (2023): June 2023
Publisher : P2M Politeknik Pertanian Negeri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51967/tanesa.v24i1.2004

Diabetes mellitus is a metabolic disease characterized by high blood glucose levels. The cause of glucose control failure is decreased insulin production by pancreatic β-cells and insulin resistance. Both lead to the obstacle of glucose uptake into cells. The mechanism of glucose uptake into cells is crucial in carbohydrate metabolism. This mechanism aims to produce energy in the form of ATP. The main signaling pathway after the process of glucose uptake is the PI3K/Akt pathway. This pathway involves many proteins activated by phosphorylation mechanisms. One of the proteins involved in this pathway is PTEN, a PI3K regulator. PTEN activity can dephosphorylate PI3K so that the insulin signaling pathway becomes blocked and glucose cannot be uptaken into cells. It causes blood glucose levels to increase. The role of PTEN in inhibiting the PI3K/Akt pathway seems to be a crucial matter to observe. By inhibiting PTEN activity, the insulin signaling pathway is expected to work properly. We have searched, read, analyzed, and summarized various studies regarding the potential of PTEN to reduce diabetes mellitus cases. In some research articles, the use of active compounds and therapy using stem cells to inhibit PTEN activity has shown good progress in the insulin signaling pathway. Based on these, it can be an option to make PTEN a target for diabetes mellitus therapy.

PTEN: The Potential Therapeutic Target of Diabetes Mellitus Ni Made Wiasty Sukanty; I Putu Bayu Agus Saputra; Lina Yunita
Poltanesa Vol 24 No 1 (2023): June 2023
Publisher : P2M Politeknik Pertanian Negeri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51967/tanesa.v24i1.2004

Diabetes mellitus is a metabolic disease characterized by high blood glucose levels. The cause of glucose control failure is decreased insulin production by pancreatic β-cells and insulin resistance. Both lead to the obstacle of glucose uptake into cells. The mechanism of glucose uptake into cells is crucial in carbohydrate metabolism. This mechanism aims to produce energy in the form of ATP. The main signaling pathway after the process of glucose uptake is the PI3K/Akt pathway. This pathway involves many proteins activated by phosphorylation mechanisms. One of the proteins involved in this pathway is PTEN, a PI3K regulator. PTEN activity can dephosphorylate PI3K so that the insulin signaling pathway becomes blocked and glucose cannot be uptaken into cells. It causes blood glucose levels to increase. The role of PTEN in inhibiting the PI3K/Akt pathway seems to be a crucial matter to observe. By inhibiting PTEN activity, the insulin signaling pathway is expected to work properly. We have searched, read, analyzed, and summarized various studies regarding the potential of PTEN to reduce diabetes mellitus cases. In some research articles, the use of active compounds and therapy using stem cells to inhibit PTEN activity has shown good progress in the insulin signaling pathway. Based on these, it can be an option to make PTEN a target for diabetes mellitus therapy.

Edukasi Label Informasi Nilai Gizi Sebagai Upaya Peningkatan Pengetahuan dan Kemampuan Membaca Label Gizi Siswa di SMAN 5 Mataram: Education on Nutritional Value Information Labels as an Effort to Increase Knowledge and Ability to Read Nutrition Labels for Students at SMAN 5 Mataram Novianti Tysmala Dewi; Lina Yunita; Ni Made Wiasty Sukanty; Farida Ariani
Jurnal Mandala Pengabdian Masyarakat Vol. 4 No. 1 (2023): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi STIKES Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v4i1.225

Label informasi nilai gizi adalah label kemasan yang memuat daftar kandungan gizi makanan dan komponen lainnya seperti takaran saji, jumlah takaran saji, dan persen angka kecukupan gizi. Data Badan Perlindungan Konsumen Nasional (BPKN) menunjukkan bahwa hanya 7,9% konsumen yang memperhatikan dan membaca label informasi nilai gizi. Kurangnya pengetahuan tentang label informasi nilai gizi dapat memengaruhi pola konsumsi, menyebabkan kegemukan, dan obesitas. Remaja tergolong salah satu konsumen yang paling aktif dalam mengonsumsi makanan kemasan. Salah satu cara untuk meningkatkan pengetahuan remaja tentang informasi nilai gizi adalah dengan memberikan edukasi. Selain bertujuan untuk meningkatkan pengetahuan dan kemampuan siswa, edukasi terkait label gizi juga bertujuan untuk meningkatkan kesadaran dan mengubah perilaku untuk mencapai kesehatan dan gizi yang optimal. Edukasi, pretest, dan posttest terkait informasi nilai gizi diberikan kepada 26 siswa SMAN 5 Mataram yang tergabung dalam kelompok UKS dan PMR. Hasil kegiatan menunjukkan bahwa pengetahuan dan kemampuan membaca label informasi nilai gizi siswa meningkat setelah diberikan edukasi. Dengan demikian, pemberian edukasi ini memberikan hasil yang positif. Melalui kegiatan ini, kami berharap para siswa semakin sadar dan mampu memilih jajanan sehat untuk mencapai gizi yang optimal.

PROTEIN RAS-LIKE PROTEINS FROM RAT BRAIN SEBAGAI TARGET PENGHAMBATAN PERKEMBANGAN SEL KANKER NI MADE WIASTY SUKANTY; I PUTU BAYU AGUS SAPUTRA; FARIDA ARIANI; LINA YUNITA; NOVIANTI TYSMALA DEWI
GANEC SWARA Vol 17, No 4 (2023): Desember 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i4.661

Ras-like proteins from rat brain (Rab) is one of the crucial proteins in the process of intracellular transport. Rab plays a role in delivering vesicles from the donor membrane to the acceptor membrane. Vesicles contain various molecules that are important in cell growth and development. These molecules are generally involved in some signaling pathways. In carrying out its functions, Rab needs to be activated first. The activation process of Rab involves various proteins. Thus, Rab activation and deactivation control are critical to regulating the movement of the transferred molecules. In cancer, there is an uncontrolled activation and deactivation of Rab. Rab's involvement in various signaling pathways by providing molecules needed for growth and development is a basis for increasing the speed of growth and development of cancer cells. Although Rab is not the main factor causing cancer, it can help the development of cancer. So, it can be one of the keys to controlling the transfer of molecules involved in the growth and development of cancer cells. There are several methods related to the activation of Rab for molecular transport. Rab activation inhibition by Rab inhibitors and inhibitors of Rab activating proteins and the use of miRNAs can prevent molecule transport. Thus, control of Rab activation can be an alternative to suppress the growth and development of cancer cells.

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