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All Journal Biogenesis: Jurnal Ilmiah Biologi Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Makara Journal of Science
Dwi Umi Siswanti, Dwi Umi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The Effect of Acidic pH on Growth Kinetics, Biomass Productivity, and Prima-ry Metabolite Contents of Euglena sp. Nurafifah, Istini; Hardianto, Muhammad Andhi; Erfianti, Tia; Amelia, Ria; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Siswanti, Dwi Umi; Sadewo, Brilian Ryan; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, and 4.5. The results were analyzed by one-way analysis of variance at a 95% confidence level, followed by Duncan’s multiple range test. As results, Euglena sp. had the best growth rate, the greatest biomass, and the highest carbohydrate, protein, and lipid contents at pH 3.5 compared to the other pH conditions. The average biomass in the pH 3.5 treatment was 1.600 ± 0.229 g/L, and the carbohydrate, protein, and lipid contents were 5.983 ± 0.056 g/L, 0.196 ± 0.023 µg/mL, and 0.300 ± 0.020 g/L, respectively.
The Development of Antioxidant Nutraceuticals containing Chrysanthemum indicum L. Gummy Candy Rahmasari, Firdaus Salvia; Sahid, Muhammad Novrizal Abdi; Siswanti, Dwi Umi; Darsih, Cici; Utami, Indrawati Dian; Alam, Lucky Prabowo Miftachul; Laksitorini, Marlyn Dian
Majalah Obat Tradisional Vol 29, No 1 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87112

Abstract

Chrysanthemum flowers (Chrysanthemum indicum L.) have been shown to contain flavonoid compounds and exert antioxidant activity. This brings Chrysanthemum indicum potential to be developed as a nutraceutical product. This study aims to evaluate the antioxidant activity of Chrysanthemum flowers before and after the formulation of Chrysanthemum indicum flower extract as a gummy candy. Gummy candy formulas were developed using variations of gelatin and pectin as gelling agents. In this study, Chrysanthemum flower extract was formulated into gummy candy. The physical characteristics evaluated include organoleptic tests, weight uniformity, elasticity, and moisture content. Optimization was performed using the simplex lattice design (SLD) method with the aid of the Design Expert software Ver. 13. The antioxidant activity of the chrysanthemum flower extract and gummy candy extract was evaluated using the DPPH radical scavenging method. Ascorbic acid was used as a positive control. The optimum formula for preparing the gummy candy was 11.51% of gelatin and 1.24% of pectin. The evaluation of weight uniformity, elasticity, and moisture content suggested that there is no significant difference between the optimum formula and the predicted value. Both the chrysanthemum flower extract and chrysanthemum flower gummy candy had strong antioxidant activity. The IC50 value of the extract was 67.80 ± 2.37 mg/mL while the gummy candy IC50 value was 82.93 ± 2.55 mg/mL. The antioxidant activity of Chrysanthemum indicum was slightly decreased after being formulated into gummy candy. These studies suggested that scientists are expected to anticipate the decrease of Chrysanthemum antioxidant activity in the gummy manufacturing process.
Response of root anatomy and vitamin C content of Brassica juncea L. on biofertilizer application in a saline environment Palupi, Dwi Rekno; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 2 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i2.36522

Abstract

Indian mustard (Brassica juncea L.) is part of the family Brassicaceae which has an annual-herbaceous habitus and is counted as a popular vegetable commodity in Indonesia so it has a high-demand market. To fulfill the demand market can be done by hydroponic cultivation and biofertilizer application. Application of biofertilizer which contains inoculant microorganisms can support plant growth with increased availability of nutrients in planting medium and neutralized salinity stress effect. Salinity stress is indicated by the high concentration of Na+ and Cl- ions in plants resulting in toxicity and osmotic stress which will inhibit plant growth. This study aimed to determine the effect of various dosages of biofertilizer on plant growth and productivity with plant height, leaf number, cortex thickness, metaxylem diameter, and ascorbic acid levels as parameters. This research used cow urine base biofertilizer containing nitrogen (N), phosphate (P), kalium (K), and calcium (Ca). There are a few treatments: negative control, positive control, biofertilizer treatment 40 mL, 80 mL, and 120 mL dosage, and salinity treatment by applying NaCl 5000 ppm 10 mL/system. As the result showed on 120 mL biofertilizer dosage has the highest mean of plant height and leaves number, and 80 ml biofertilizer dosage treatment has the highest average of cortex thickness and metaxylem diameter, but the various dosage of biofertilizer treatment did not have a significant effect on ascorbic acid levels increase. Growth in plants given biofertilizer is higher than the plant growth that grew in the negative control treatment. It can be concluded that the application of biofertilizer can support the growth of B. juncea L. in the hydroponic system under salinity stress.
Bioremediation of Mercury- Polluted Water in Free Water Surface-Constructed Wetland System by Euglena sp. and Echinodorus palifolius (Nees & Mart.) J.F. Macbr. Siswanti, Dwi Umi; Daryono, Budi Setiadi; Petrus, Himawan Tri Bayu Murti; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88143

Abstract

Mercury accumulation in the aquatic environment can be highly harmful. The body takes mercury vapor through the lungs, then absorbs mercury metal through the digestive system, and then the blood carries the metal to the brain. Bioremediation is the process of breaking down or converting harmful compounds into non-toxic forms, which can be accomplished through phytoremediation or phycoremediation. The goal of this study was to examine the growth and anatomy of Euglena sp. after being cultured in the mercury-containing FWS-CW waste treatment system. The ability of Euglena sp. and Echinodorus palifolius to bioremediate mercury at different concentration as well as association and non-association treatments. This study was carried out in a bioreactor known as FSW-CW (Free Water Surface-Constructed Wetlands). Plant growth (plant height and number of leaves), chlorophyll content, diameter of root and petiole, metaxylem diameter of root, petiole, and leaves, cortical thickness of root and leaves, and petiole anatomy were all measured. Water temperature, pH, salinity, and light intensity were all measured as environmental parameters. Mercury treatment reduced Euglena density (183.5 cells. mL-1103 in control and 12.6 cells. mL-1103 in 100 ppm mercury treatment) and number of E. palifolius leaves, but not plant height and chlorophyll. Root and petiole diameters were affected by the mercury treatment, petiole diameter decreased unless the concentration was 100 ppm, whereas root diameter actually increased. The diameter of the root metaxylem increased, but the petioles and leaves, as well as the thickness of the root cortex, did not provide a significant response. The growth of E. palifolius was still optimal in the presence of Euglena in mercury-containing medium. 
Co-Authors Alam, Lucky Prabowo Miftachul Amelia, Ria BUDI SETIADI DARYONO Cici Darsih Eko Agus Suyono Erfianti, Tia Hardianto, Muhammad Andhi Himawan Tri Bayu Murti Petrus Kurnianto, Dedy Maghfiroh, Khusnul Qonita Marlyn Dian Laksitorini, Marlyn Dian Nurafifah, Istini Palupi, Dwi Rekno Putri, Renata Adaranyssa Egistha Rahmasari, Firdaus Salvia Sadewo, Brilian Ryan Sahid, Muhammad Novrizal Abdi Utami, Indrawati Dian