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DETEKSI URUTAN OLIGONUKLEOTIDA Mycobacterium tuberculosis SECARA VOLTAMMETRI MENGGUNAKAN SCREEN PRINTED CARBON ELECTRODE (SPCE) Hartati, Yeni Wahyuni; Yohan, Yohan; Nurmalasari, Ratna; Gaffar, Shabarni; Lubis, Rubianto A.
Chimica et Natura Acta Vol 4, No 2 (2016)
Publisher : Departemen Kimia

Mycobacterium tuberculosis merupakan bakteri penyebab tuberkulosis (TB). Pengembangan analisis secara biosensor DNA sangat menarik perhatian karena penerapannya mudah. Dalam penelitian ini telah dilakukan penentuan urutan pendek oligonukleotida M. tuberculosis gen RV0508 dari strain H37RV secara voltammetri pulsa differensial menggunakan screen printed carbon electrode (SPCE). Pendeteksian berdasarkan teknik hibridisasi urutan oligonukleotida probe yang diadsorpsi pada permukaan SPCE, dengan pasangan komplementernya dari DNA target, tanpa indikator hibridisasi, yaitu dengan mensubstitusi basa guanin probe dengan basa inosin. Respon hibridisasi berupa signal guanin DNA target di daerah potensial sekitar +0,9 V. Telah diperoleh korelasi linear antara arus puncak dengan konsentrasi DNA target pada rentang 5,0-20,0 Âµg/mL dengan batas deteksi 8,2 Âµg/mL.

DETEKSI URUTAN OLIGONUKLEOTIDA Mycobacterium tuberculosis SECARA VOLTAMMETRI MENGGUNAKAN SCREEN PRINTED CARBON ELECTRODE (SPCE) Yeni Wahyuni Hartati; Yohan Yohan; Ratna Nurmalasari; Shabarni Gaffar; Rubianto A. Lubis
Chimica et Natura Acta Vol 4, No 2 (2016)
Publisher : Departemen Kimia

Mycobacterium tuberculosis merupakan bakteri penyebab tuberkulosis (TB). Pengembangan analisis secara biosensor DNA sangat menarik perhatian karena penerapannya mudah. Dalam penelitian ini telah dilakukan penentuan urutan pendek oligonukleotida M. tuberculosis gen RV0508 dari strain H37RV secara voltammetri pulsa differensial menggunakan screen printed carbon electrode (SPCE). Pendeteksian berdasarkan teknik hibridisasi urutan oligonukleotida probe yang diadsorpsi pada permukaan SPCE, dengan pasangan komplementernya dari DNA target, tanpa indikator hibridisasi, yaitu dengan mensubstitusi basa guanin probe dengan basa inosin. Respon hibridisasi berupa signal guanin DNA target di daerah potensial sekitar +0,9 V. Telah diperoleh korelasi linear antara arus puncak dengan konsentrasi DNA target pada rentang 5,0-20,0 µg/mL dengan batas deteksi 8,2 µg/mL.

Utilization of Nata De Coco as Adsorben in Methyl Orange Adsorption Sani Widyastuti Pratiwi; Siska Novita Sari; Ratna Nurmalasari; Meli Indriani
EduChemia (Jurnal Kimia dan Pendidikan) Vol 5, No 2 (2020)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Methyl Orange was a textile waste that polluted the environment and harmed aquatic ecosystems and human life. It could affect the photosynthesis of oxygen regeneration and disrupt the biological activity of microbes in the water. One of the ways to deal with these wastes by adsorption using Nata de coco. Nata de coco was used as an alternative adsorbent made from cellulose, which was a waste of coconut water in the process of making it easy, inexpensive, and biodegradable. This study aims to determine the effect of contact time and mass of Nata de coco adsorbents on the adsorption of methyl orange. This research method includes three stages were adsorbent preparation, adsorption, and UV-Vis spectrometer analysis. The adsorption process was carried out with contact time variations of 30 and 60 minutes, variations in the mass of the adsorbent 0.05g; 0.1g; and 0.15g. The results showed the optimum adsorption conditions occurred at 60 minutes of contact time and an adsorbent mass of 0.15g with an adsorption efficiency of 44.66% and an adsorption capacity of 0.2066 mg/g.

Optimasi Pembuatan Mikrokapsul Kalsium-Alginat-EDTA Sebagai Adsorben Untuk Logam Kadmium Sani Widyastuti Pratiwi; Ayu Triastuti; Ratna Nurmalasari; Inggis Pinarti
Jurnal Pijar Mipa Vol. 15 No. 4 (2020): September
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram

Cadmium was one of the metals classified in the type of nonessential heavy metal that classified as carcinogenic and is one of the pollutants in the environment, especially water. The content of cadmium in water tends to have very low levels, so certain techniques are needed to determine the level of pre concentration. In the pre concentration technique adsorbents were needed which could absorb cadmium, one of which is calcium-alginate microcapsuleÃÂ modified with EDTA. Calcium-alginate wasÃÂ modified with EDTA to increase the ability of the microcapsule to absorb cadmium metal. The purpose of this study was to determine the optimum conditions for making Calcium-alginate-EDTA microcapsuleÃÂ in adsorbing cadmium. The stages of this research were optimization of Calcium-alginate-EDTA resin with variable in the form of Calcium chloride concentration (0.05 M; 0.1 M; 0.5 M; 1 M; and 2 M), EDTA mass (0.50 g ; 0.75 g; 1 g; 2 g and 2.5 g) and the concentration of sodium-alginate (0.5%; 1%, 1.5%; 2%, and 3%); Characterization of microcapsuleÃÂ functional groups by infrared spectrophotometry and determination of the retention capacity of Calcium-Alginate-EDTA microcapsuleÃÂ for cadmium absorption by atomic absorption spectrophotometry. The results of infrared characterization showed a change in transmittance in the functional groups C-O dan C-N that occurred in the microcapsuleÃÂ after binding to cadmium metal at wave number 1436.97 cm-1. The optimum conditions for making Calcium-alginate-EDTA resins were in conditions of 0.1 M Calcium chloride concentration, 1% sodium-alginate concentration and EDTA mass 0.75 g EDTA with retention capacity ofÃÂ Cd was 0.0301 mg/g.

An Electrochemical Immunosensor for the Detection of B-Type Natriuretic Peptide (BNP) Based on Sandwich ELISA Using Screen Printed Carbon Electrodes Yeni Wahyuni Hartati; Muhammad Hilman Daniswara; Ratna Nurmalasari; Shabarni Gaffar; Toto Subroto
Molekul Vol 13, No 1 (2018)
Publisher : Universitas Jenderal Soedirman

B-type natriuretic peptide (BNP) is a 32-amino acid polypeptide, a cardiac neuro hormone that specifically secreted from heart ventricle as a response towards the increase of volume and pressure in the heart. The determination of BNP concentration in patients blood is one of the method used to diagnose heart failure. An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes for the detection of the BNP antigen was developed in this study. Monoclonal anti-BNP capture antibody was immobilized on streptavidin-modified SPCEs to give a well oriented of antibody. Furthermore, a biotinylated anti-BNP that conjugated with horseradish peroxidase (HRP) was used as secondary antibody. The electrochemical signal produced by redox activity of substrate 3,3,5,5'-tetramethybezidine dihydrochloride (TMB/H2O2) was measured by differential pulse voltammetry. The BNP immunosensor showed a linear response between 1.0×10-2 and 1.0×102 ng/mL, and the limit of detection was 3.3 ng/mL. BNP immunosensor is a promising technology for the rapid and convenient detection of heart failure.

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