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SNP G-1082A IL-10 GENE: ALLELE AND GENOTYPE DISTRIBUTION OF PERIODONTITIS PATIENT IN YOGYAKARTA: SNP G-1082A GEN IL-10: DISTRIBUSI ALEL DAN GENOTIP PADA PASIEN PERIODONTITIS DI YOGYAKARTA Sari, Rezmelia; Prayitno, Prayitno; Fadhilah, Alya Nur
Dentika: Dental Journal Vol. 19 No. 2 (2016): Dentika Dental Journal
Publisher : TALENTA

Periodontitis is multifactorial inflamation process and related to disproportion of cytokine. IL-10 is a dominant noninflammatory cytokines that related to gene polymorphism. Polymorphism G-1082A IL-10 genes has been reported to increase the risk of periodontitis occurs in Italian populations, apart from different result found in Brazilian. The purpose of this research was to determine the polymorphism G-1082A IL-10 in periodontitis patients in Indonesia, especially among Yogyakarta’s Javanese. This is a case-control research with subjects according to the inclusion criteria. DNA was taken by cotton swab from the epithelial cells of buccal mucosa, and was isolated using a PrestoTM (GeneAid) kit. Genotyping analysis by using the PCR RFLP technique and descriptive results were presented. The result showed that A allele frequency is 100% and no G allele was found. AA genotype in case group has lower frequency than in control group and vice versa. From this research, it was concluded that A allele was dominant in Yogyakarta’s Javanese, and AA genotype frequency is lower in individual with periodontitis.

Prevalensi periodontitis pada pasien diabetes mellitus (Studi observasional di poliklinik penyakit dalam RSUP Dr. Sardjito) Rezmelia Sari; Dahlia Herawati; Rizky Nurcahyanti; Pramudita Kusuma Wardani
Majalah Kedokteran Gigi Indonesia Vol 3, No 2 (2017): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Prevalence of periodontal diseases in patients with diabetes mellitus (An observational study at internal medicine polyclinic in Dr. Sardjito General Hospital). Diabetes Mellitus (DM) is a chronic disease with an increasing prevalence and causes complications. The most frequent complication found in the oral cavity of patients with diabetes mellitus is periodontal diseases is characterized by the loss of tissue attachment. There have been numerous studies on the association of DM with periodontal diseases but there has not been any data on the prevalence of periodontal diseases in diabetic group, especially in Yogyakarta and Central Java. Dr. Sardjito General Hospital is a referral hospital in DIY and Central Java, so this study is expected to provide a picture regarding the level of periodontal tissue health among people in Yogyakarta and Central Java. This research was an observational study, involving 36 patients with DM according to criteria of the subjects: suffering from type 2 diabetes, being cooperative and willing to sign an informed consent. The controlled variables: being 40 – 60 years of age, having good oral hygiene (OHI) according to Green and Vermillion, taking neither antibiotics nor anti-inammatory drugs in the last 3 months, not having a history of other systemic diseases. Oral hygiene exams were carried out, followed by examination using probe WHO to determine if there is CAL. The data were presented descriptively. The results showed that the prevalence of periodontal diseases in patients with DM at Internal Medicine Polyclinic in Dr. Sardjito General Hospital is 88.24% with a mean of CAL distance of 4.6 mm. The conclusion of this study is that the prevalence of periodontal diseases in patients with DM is high although the oral hygiene status is good. ABSTRAKDiabetes Mellitus (DM) adalah penyakit kronis menahun dengan prevalensi yang semakin meningkat dan menimbulkan komplikasi. Komplikasi yang paling sering terjadi di rongga mulut pasien DM adalah periodontitis yang ditandai dengan kehilangan perlekatan jaringan. Penelitian tentang hubungan DM dengan periodontitis banyak dilakukan namun belum ditemukan data mengenai prevalensi periodontitis pada kelompok DM khususnya di DIY dan Jawa Tengah. RSUP Dr. Sardjito merupakan rumah sakit rujukan DIY dan Jawa Tengah sehingga penelitian ini diharapkan dapat memberi gambaran mengenai tingkat kesehatan jaringan periodontal di masyarakat DIY dan Jawa Tengah. Jenis penelitian ini adalah observasional dengan melibatkan 36 orang pasien DM sesuai kriteria subjek yaitu menderita DM tipe II, kooperatif dan bersedia menandatangani informed consent. Variabel terkendali yaitu usia 40 – 60 tahun, kebersihan mulut (OHI) menurut Green and Vermillion dalam kriteria baik, tidak menggunakan antibiotik dan antiinamasi dalam 3 bulan terakhir dan tidak memiliki riwayat penyakit sistemik lain. Dilakukan pemeriksaan kebersihan mulut dilanjutkan dengan pemeriksaan menggunakan probe WHO untuk menentukan ada tidaknya CAL. Data disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa prevalensi periodontitis pada pasien DM di Poli Klinik Penyakit Dalam RSUP Dr. Sardjito adalah 88,24% dengan rata-rata jarak CAL adalah 4,6 mm. Kesimpulan penelitian ini adalah prevalensi periodontitis pada pasien DM tinggi walaupun status kebersihan mulut tergolong dalam kriteria baik.

Potency of stored DNA derived from buccal mucosal epithelial cells for genotype analysis of interleukin 4 -590 C/T Rezmelia Sari; Alya Nur Fadhilah; Prayitno Prayitno
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37361

The genotype variation of the interleukin 4 (IL-4) gene on spot -590 C/T is known to correlate with IL-4 protein expression in periodontitis, but the results found in the population are inconsistent. Sequencial genotype analysis of multiple genes often used stored DNA samples obtained from buccal mucosal epithelial cells. This study aims to determine the use of stored DNA samples obtained from buccal mucosal epithelial cells for genotype analysis specifically on -590 C/T IL-4 genes. This is a descriptive analytical study. The samples were 58 DNA derived from the buccal mucosal epithelial cells taken by sterile cotton swab isolated with PrestoTM kit (GeneAid). The DNA has been stored for 2 years at -20 °C. The genotype analysis was conducted using PCR RFLP technique with BSmFI enzyme and the data were presented descriptively. The results show that 51.72% of the stored DNA is still in good condition and 37.9% can be used for genotype analysis. It was concluded that DNA derived from buccal mucosal epithelial cells stored at -20 °C for 2 years can still be used for genotype analysis, but the quality of DNA is affected by storage time.

DISCLOSING AGENT FROM RED DRAGON FRUIT PEEL AS DENTAL PLAQUE INDICATOR Eky Purbaningtyas; Fifin Yuliani; Arief Putra Ananda; Rezmelia Sari
ODONTO : Dental Journal Vol 7, No 1 (2020): July 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.1.31-39

Background: Red dragon fruit peel mucoadhesive gel has the potential to dye dental plaque. The colour of a transparent dental plaque can be seen with visualize using dyes (disclosing agent). One of the natural products containing dyestuff betacyanin, namely the peel of the red dragon (Hylocereus polyrhizus). This research aimed to review the peels of red dragon betacyanin (Hylocereus polyrhizus) in the mucoadhesive gel formula as an alternative disclosing agent.Method: Research is done with the manufacture of thick peels of a red dragon extract with the maceration methods. In clinical trials carried out an application red dragon fruit peel mucoadhesive gel 10%, concentration 15 % and Three-Tone Plaque Disclosing Gel. The assessment of plaque was carried out using the O’leary method. Result: The results obtained were analyzed by the t-test method. The mean of plaque index scores with red dragon fruit peel mucoadhesive gel concentrations of 10% (22.08), 15% (26,29) and Three-Tone Plaque Disclosing Gel (66,21; 64,20). The effectiveness of the gel with a concentration of 10% is 33.87%, and 15% concentration is 40.32%. Conclusion: The mucoadhesive gel of red dragon fruit peel has the potential to colour dental plaque.

THE EFFECT OF BIOLOGICAL MEMBRANES TO FIBROBLAST PROLIFERATION: PLATELET-RICH FIBRIN RELEASATE VS AMNIOTIC (Research report) Rezmelia Sari; Sri Pramestri Lastianny; Alya Nur Fadhilah
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6181

Background: Both amniotic and platelet-rich fibrin membranes are used to enhance open wound healing process clinically. The growth factors of these membranes are almost similar but the certain mechanism is still unclear especially in inducing fibroblast proliferation. Objective: to compare the effect of both membranes to fibroblast proliferation. Methods: This in vitro study was using fibroblast from periodontal ligament. The fibroblasts were cultured and then divided into 3 groups: fibroblasts with PRF releasate membrane (group I), fibroblasts only (group II), and fibroblasts with amniotic membranes (group III). The cells were observed in 24, 48 and 72 hours. Proliferation was tested by MTT assay and data was analyzed by two-way ANOVA followed by post-hoc test. Results: It showed that PRF releasate membrane induced fibroblast proliferation higher than amniotic in the first 24 hours meanwhile amniotic induced it two-fold in the next 24 hours. However, in the following time there were no significant differences between groups. Conclusion: It can be concluded that PRF membranes and amniotic membranes have the ability to accelerate proliferation of fibroblasts but have different effects at the time of induction.

Topical application of snail mucin gel enhances the number of osteoblasts in periodontitis rat model H. Hendrawati; Hanindya Noor Agustha; Rezmelia Sari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD. Results: A significant difference existed between the number of osteoblasts in the test groups. The highest number of osteoblasts observed was consistently that in the treatment group. Conclusion: The application of 20% snail mucin gel was effective in enhancing the number of osteoblasts in rats suffering from periodontitis.

DISCLOSING AGENT FROM RED DRAGON FRUIT PEEL AS DENTAL PLAQUE INDICATOR Eky Purbaningtyas; Fifin Yuliani; Arief Putra Ananda; Rezmelia Sari
Odonto : Dental Journal Vol 7, No 1 (2020): July 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Red dragon fruit peel mucoadhesive gel has the potential to dye dental plaque. The colour of a transparent dental plaque can be seen with visualize using dyes (disclosing agent). One of the natural products containing dyestuff betacyanin, namely the peel of the red dragon (Hylocereus polyrhizus). This research aimed to review the peels of red dragon betacyanin (Hylocereus polyrhizus) in the mucoadhesive gel formula as an alternative disclosing agent.Method: Research is done with the manufacture of thick peels of a red dragon extract with the maceration methods. In clinical trials carried out an application red dragon fruit peel mucoadhesive gel 10%, concentration 15 % and Three-Tone Plaque Disclosing Gel. The assessment of plaque was carried out using the O’leary method. Result: The results obtained were analyzed by the t-test method. The mean of plaque index scores with red dragon fruit peel mucoadhesive gel concentrations of 10% (22.08), 15% (26,29) and Three-Tone Plaque Disclosing Gel (66,21; 64,20). The effectiveness of the gel with a concentration of 10% is 33.87%, and 15% concentration is 40.32%. Conclusion: The mucoadhesive gel of red dragon fruit peel has the potential to colour dental plaque.

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