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Pengaruh Aplikasi Gel Ekstrak Kulit Citrus Sinensis terhadap Epitelisasi pada Penyembuhan Luka Gingiva Tikus Sprague Dawley Aqilla Tiara Kartiningtyas; Prayitno Prayitno; Sri Pramestri Lastianny
Majalah Kedokteran Gigi Indonesia Vol 1, No 1 (2015): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Penyembuhan luka merupakan mekanisme pertahanan jaringan, dengan epitelisasi sebagai salah satu parameternya. Kulit jeruk manis memiliki potensi dalam penyembuhan luka karena mengandung nutrisi yang memiliki peran dalam mempercepat penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efek aplikasi topikal gel ekstrak kulit jerukmanis terhadap epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Dua puluh tujuh ekor tikus Sprague Dawley, berumur 2-3 bulan, dibagi dalam 3 kelompok, kontrol positif, perlakuan, dan kontrol negatif. Perlukaan pada gingiva labial mandibula dibuat dengan punch biopsy berdiameter 2,5 mm. Luka pada kelompok kontrolpositif diaplikasikan Aloclair, kelompok perlakuan diaplikasikan gel ekstrak kulit jeruk manis 10%, dan kelompok kontrol negatif diaplikasikan CMC-Na, masing-masing 2 kali sehari selama 1 menit secara topikal. Tiga ekor tikus dari tiap kelompok didekapitasi masing-masing pada hari ke-3, 7, dan 14. Jaringan luka diambil dan dibuat sediaan histologis dengan pengecatan Hemaktosilin Eosin. Pengukuran ketebalan epitel dilakukan dengan menggunakan Optilab yang dipasang pada mikroskop cahaya. Data ketebalan epitel dianalisis dengan menggunakan ANAVA dua jalur. Hasil pengukuran ketebalan epitel menunjukkan perbedaan yang tidak signifikan pada kelompok kontrol positif dengan perlakuan (p>0,05), namun menunjukkan perbedaan yang signifikan pada kelompok perlakuan dengan kontrol negatif (p<0,05) pada hari 3, 7, dan 14 setelah perlukaan. Kesimpulan dari penelitian ini adalah aplikasi topikal gel ekstrak kulit jeruk manis mempercepat epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Effect of Topical Application of Sweet Orange (Citrus sinensis) Peel Extract Gel on Epithelialization of Labial Gingival Wound Healing: In Vivo Studies in Sprague Dawley Rats. Wound healing is a defense mechanism from complex biological phenomenon, in which epithelialization occurs as one of its parameters. Sweet orange peelcontains nutrients that have role in enhancing wound healing process. The objective of this research is to determine the effect of topical application of sweet orange peel extract gel on epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats. Twenty seven Sprague Dawley rats were divided into 3 groups: positivecontrol, treatment, and negative control. Labial mandibular gingival was wounded using 2.5 mm diameter punch biopsy. Topically, each wound of positive control group was administered Aloclair, treatment group was administered 10% sweet orange peel extract gel, and negative control group was administered CMC-Na, twice a day for 1 minute. Three rats from each group were sacrificed for histological evaluation at 3, 7, and 14 days and the specimens were stained with HE. The measurement of the epithelial thickness used Optilab installed on microscope. The data obtained from the measurement was analyzed using two-way ANOVA. There was insignificant difference between positive control group and treatment group (p>0.05) while there was significant difference between treatment group and negative control group (p<0.05) observed at 3, 7, and 14 days. The conclusion of this research is that the application of sweet orange peel extract gel accelerates epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats.

Dampak Pemakaian Alat Ortodontik terhadap Kesehatan Jaringan Periodontal Sri Pramestri Lastianny
Majalah Kedokteran Gigi Indonesia Vol 19, No 2 (2012): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Pendahuluan. Perawatan ortodontik terutama yang cekat sudah banyak digunakan, bahkan akhir-akihir ini tidak hanya untuk kepentingan perawatan maloklusi gigi, tapi juga untuk estetika. Namun banyak pasien mengeluh adanya peradangan, bau mulut bahkan sampai terjadinya periodontitis berat, sampai kegoyahan gigi. Tujuan penulisan. Untuk mengetahui dampak yang ditimbulkan dari pemakaian alat ortodontik terhadap kesehatan jaringan periodontal. Dampak tersebut ditinjau dari efek alat ortodontik yang dapat meningkatkan akumulasi plak, iritasi yang ditimbulkan pada pemakaian band dan braket pada alat ortodontik, juga akibat tekanan yang ditimbulkan pada jaringan periodontal. Kesimpulan. Untuk meminimalkan dampak pemakaian alat ortodontik tersebut, seharusnya ditekankan pentingnya plak kontrol, juga harus dipertimbangkan tekanan yang diberikan pada jaringan periodontal. Introduction. Fixed orthodontic appliances is widely used, not only for treatment dental malacclusion, but also for aestetically. But many patient complain of gingival inflamation, severe periodontitis, event to luxation tooth. The aim of this review. Was to know the side effects of orthodontic appliances for periodontal healthy. Which was evaluated the effect of orthodontic appliances could increased the number of plaque retention, irritation of use appliances and also the pressure of this orthodontic appliances on periodontal tissues. The conclusion. To minimize the side effect of using orthodontic appliances, beside instruction for controle plaque, also consider the pressure exerted for periodontal tissues.

Penatalaksanaan Hiperpigmentasi Gingiva dan Mahkota Gigi Klinis Pendek dengan Surgical Scalpel Technique pada Gigi Anterior Ade Ismail A. K.; Sri Pramestri Lastianny
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar belakang. Warna gingiva normal, mencerminkan kesehatan mulut dan mempunyai peranan penting dalam kedokteran gigi estetik. Jika terdapat kelainan warna, seperti gingiva yang kehitam-hitaman yang sering dikeluhkan pasien, dapat merupakan suatu masalah walau bukan suatu masalah medis. Kadang-kadang pewarnaan gingiva ini diperparah dengan adanya mahkota gigi klinis yang pendek. Ada beberapa macam teknik depigmentasi untuk mengatasi masalah ini. Tujuan. Laporan kasus ini bertujuan menghilangkan hiperpigmentasi gingiva dengan menggunakan teknik sederhana dalam waktu minimal serta tidak terlalu sulit dilakukan tapi masih cukup memuaskan bagi pasien. Kasus. Pasien perempuan umur 18 tahun mengeluh gingiva berwarna gelap dan mahkota gigi tampak pendek yang mengganggu penampilan dan ingin diperbaiki. Diagnosis dari kasus ini adalah hiperpigmentasi gingiva dengan mahkota gigi klinis pendek. Pada kasus ini digunakan teknik bedah skalpel untuk mengkoreksi hiperpigmantasi gingiva dan mahkota gigi klinis pendek tersebut. Pasien sangat puas dengan penampilan gingivanya yang baru. Kesimpulan. Teknik bedah skalpel dapat mengatasi hiperpigmentasi gingiva dan mahkota gigi klinis pendek.

Differences in results of infrabony pocket treatment with addition of platelet rich fibrin and platelet rich plasma gel in DFDBA bone graft Netta Anggraini; Sri Pramestri Lastianny; Al Sri Koes Soesilowati
Majalah Kedokteran Gigi Indonesia Vol 5, No 1 (2019): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Treatment of infrabony pocket makes use of bone graft material demineralized freeze dried bone allograft (DFDBA) from different individuals which has undergone demineralization process and it is osteoinductive. Enhancement of growth factor was done by adding platelet rich fibrin (PRF) and platelet rich plasma (PRP). PRP is activated with an addition of calcium chloride CaCl2) to form gel. The method used to apply the bone grafting material is open flap debridement OFD). This research aimed to reveal the differences in the results of infrabony pocket treatment using PRF and PRP gel with an addition of DFDBA. The sample was taken from 20 infrabony pocket points divided into 2 groups, 10 infrabony pocket were treated with OFD+DFDBA+PRF and the other were treated with OFD+DFDBA+PRP gel. Pocket depth (PD) was measured on the baseline and the first and third month after treatment. Alveolar bone height was measured using cone beam computed tomography (CBCT) radiograph on the baseline to the third month after treatment. The results of this research showed that there was difference in the results of infrabony pocket treatment using PRF and PRP gel with an addition of DFDBA which could be observed from a reduction in PD from the baseline, month 1 and month 3 as well as reduction in alveolar bone height from the baseline to month 3. This research concluded that infrabony pocket treatment PRF application yields better results than PRP gel application in terms of PD and alveolar bone height reduction.

Differences in effectiveness of membrane fixation using periosteal vertical mattress and simple sling suture techniques in infrabony pocket treatment Beta Widya Oktiani; Sri Pramestri Lastianny; Ahmad Syaify
Majalah Kedokteran Gigi Indonesia Vol 5, No 2 (2019): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37425

Guide tissue regeneration (GTR) is the treatment of infrabony pockets for soft and hard tissue regeneration. Membrane is used as a barrier and prevents apical migration of the cells in epithelial tissues. Membrane fixation is one of the procedures in GTR treatment because resorbed membrane is less stable. Simple sling suture technique for membrane fixation has 1 anchorage, located in coronal flap, while periosteal vertical mattress suture technique has 2 anchorages in periosteum, making it more stable. This study aimed to study the differences in the effectiveness of membrane fixation using periosteal vertical mattress suture and simple sling suture techniques in terms of probing depth, relative attachment loss, and alveolar bone height in the treatment of infrabony pockets. The samples were divided into 2 groups. The first group was open flap debridement (OFD) with demineralized freeze dried bone allograft (DFDBA) application and membrane fixation with simple sling suture, while the second group was OFD with DFDBA application and membrane fixation with periosteal vertical mattress suture, observed on day 0, 30th day, and 90th day. The results of the study showed significant differences in the probing depth and relative attachment loss (except from the 30th day to the 90th day), and there were no significant differences in the alveolar bone height from the baseline to the 90th day, between the group of membrane fixation using simple sling suture and that of periosteal vertical mattress suture techniques on flap surgery. This study concluded that membrane fixation in the treatment of infrabony pocket with periosteal vertical mattress suture technique is more effective in reducing the probing depth and relative attachment loss, but there is no difference in increasing the alveolar bone height when compared to simple sling suture technique.

Effect of sandwich bone augmentation using hydroxyapatite and demineralized freeze- dried bone on infrabony pocket treatment Eka Pramudita Ramadhany; Al Sri Koes Soesilowati; Sri Pramestri Lastianny
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37427

Periodontitis is periodontal inflammation in response to plaque bacterial antigens, causing damage to periodontal ligament and alveolar bone resorption. Bone graft material combination i.e. demineralized freeze-dried bone allograft (DFDBA) and hydroxyapatite (HA) using sandwich bone augmentation (SBA) method will support each other and will be beneficial to be used as a scaffold. The body takes long time to resorb HA so this could complement DFDBA which is more easily dissolved. This study aimed to reveal the effect of bone graft addition using SBA method on the treatment of infrabony pocket with open flap debridement in terms of probing depth, relative attachment loss, alveolar bone height, and density. This study was carried out to 20 infrabony pockets, where 10 of them were treated using open flap debridement with HA addition, while the other 10 groups were treated using open flap debridement with DFDBA and HA using SBA method. Probing depth and relative attachment loss were measured on days 0, 30 and 90. Bone height and density were measured using cone-beam computed tomography (images on day 0 and 90). The study showed that probing depth reduction on SBA group was greater than HA group. There were significant differences in probing depth and relative attachment loss examinations. However, bone height and bone density reduction did not show any significant difference. The conclusion from this study is open flap debridement using SBA method yields better regeneration in terms of probing depth and relative attachment loss than open flap debridement with HA addition. There is no difference in bone height and bone density between the two groups.

Effect of collagen-chitosan hydrogel formula combined with platelet-rich plasma (A study of ph, viscocity, and swelling test) Tutut Prabantari Anindyajati; Sri Pramestri Lastianny; Flandiana Yogianti; Kwartarini Murdiastuti
Majalah Kedokteran Gigi Indonesia Vol 6, No 3 (2020): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.44391

Platelet-rich plasma (PRP) contains growth factors that speed up a healing process. The liquid form of PRP is difficult to be applied, so it needs material as a carrier of collagen. Collagen can be used as carrier materials and capable to activate growth factors and increase the concentration of the PRP to become a gel. Collagen often losses its shape and size due to degradation when exposed to body fluids. In the selection of materials, collagen can synthesize with chitosan. Collagen-chitosan hydrogel has potential as scaffold. Many formulations for proper hydrogel applied in the oral cavity need to pay more attention to several conditions, such as a neutral pH, high viscosity and ideal swelling. The aims of this study was to examine the influence of various formulations of collagenchitosan hydrogel PRPagainst pH, viscosity, and swelling. The study samples consisted of 25 collagen-chitosanhydrogel formulations. The samples were divided into five groups: 100/0; 75/25; 50/50; 25/75; 0/100. The first stage of the test was pH, viscosity and swelling measurement of the collagen-chitosan hydrogel to determine which ones matched the criteria. Collagen-chitosan hydrogel formulations were added with PRP with a ratio of 1:1. The second stage was to measure the pH, viscosity, and swelling measurement to see the results of the physical parameters of the hydrogel after mixed with PRP. The observation data were analyzed by t-test for pH and viscosity, while twoway ANOVA and post hoc LSD for swelling test. The results showed that collagen-chitosan hydrogel formulations of 25/75 and 0/100 met the criteria of pH, viscosity, and swelling. The pH of both groups showed no significant difference (p>0.05), but the viscosity and swelling variables showed significant difference (p<0.05). To conclude, collagen-chitosan hydrogel formulations mixed with PRP had no effect on pH, but had an effect on viscosity and swelling test. The collagen-chitosan hydrogel formulations mixed with PRP which matched the criteria were 25/75 and 0/100.

DIFFERENCES IN EFFECTIVENESS OF OCIMUM-SANCTUM 4% GEL AND 25% METRONIDAZOLE GEL POST SCALING ROOT-PLANING IN CHRONIC PERIODONTITIS Vincensia Maria Karina; Sri Pramestri Lastianny; Rina Meiliyanawaty
ODONTO : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.1.141-146

Background: Chronic periodontitis is a disease that causes inflammation of the tooth supporting tissue, loss of periodontal attachment, and loss of alveolar bone. Root planning scaling treatment is carried out to remove irritants and microorganisms. The use of additional materials is required when the pocket depth is more than 5 mm. One of the herbal ingredients that has the potential as an adjunct to periodontal therapy is the 4% ocinumsanctum.Method: The sample consisted of 20 periodontal pockets with a depth of 4-6 mm which were given scaling root planning which were divided into two groups, namely 10 pockets given 4% maximum sanctum gel and 10 pockets given Metronidazole gel 25%. evaluations of the papillary bleeding index, TNF-α, and IL-β were performed at baseline, and on day 21. TNF-α and IL-β levels were taken through the gingival sulcus fluid and then tested using ELISA.Result: Papillary bleeding index, TNF-α, and IL-β levels showed a significant reduction in both groups but there was no significant difference between the 4% ocimum sanctum group and Metronidazole gel.Conclusion: ocimum sanctum application 4% post scaling root planning was able to lower papillary bleeding index score, TNF-α levels, and IL-β.

THE EFFECT OF BIOLOGICAL MEMBRANES TO FIBROBLAST PROLIFERATION: PLATELET-RICH FIBRIN RELEASATE VS AMNIOTIC (Research report) Rezmelia Sari; Sri Pramestri Lastianny; Alya Nur Fadhilah
Dentino : Jurnal Kedokteran Gigi Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i1.6181

Background: Both amniotic and platelet-rich fibrin membranes are used to enhance open wound healing process clinically. The growth factors of these membranes are almost similar but the certain mechanism is still unclear especially in inducing fibroblast proliferation. Objective: to compare the effect of both membranes to fibroblast proliferation. Methods: This in vitro study was using fibroblast from periodontal ligament. The fibroblasts were cultured and then divided into 3 groups: fibroblasts with PRF releasate membrane (group I), fibroblasts only (group II), and fibroblasts with amniotic membranes (group III). The cells were observed in 24, 48 and 72 hours. Proliferation was tested by MTT assay and data was analyzed by two-way ANOVA followed by post-hoc test. Results: It showed that PRF releasate membrane induced fibroblast proliferation higher than amniotic in the first 24 hours meanwhile amniotic induced it two-fold in the next 24 hours. However, in the following time there were no significant differences between groups. Conclusion: It can be concluded that PRF membranes and amniotic membranes have the ability to accelerate proliferation of fibroblasts but have different effects at the time of induction.

The effects of zinc oxide non-eugenol and cellulose as periodontal dressings on open wounds after periodontal surgery Yoeliani Budisidharta; Ahmad Syaify; Sri Pramestri Lastianny
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p45-49

Background: Periodontal surgery forms a part of periodontal treatment that can sometimes cause open wounds, such as gingivectomy and depigmentation. Unfortunately, the healing process of open wounds can be inhibited due to bacterial infections and systemic factors. Thus, after surgery, the open wounds need to be closed with periodontal dressing. Purpose: This study aims to reveal the differences between using zinc oxide non-eugenol and cellulose periodontal dressings on open wounds after periodontal surgery. Methods: Thirty-two samples were divided into two groups. Group I consisted of 16 samples where zinc oxide non-eugenol was applied as a periodontal dressing. Similarly, Group II consisted of 16 samples where cellulose was applied as a periodontal dressing. The dressings were applied to open wounds after periodontal surgery using the split-mouth technique. Hence, zinc oxide non-eugenol was applied on the right side and cellulose was applied on the left side of the mouth. The patients’ healing index (HI) score was measured on day seven and their wound healing index (WHI) score was measured on days seven and twenty-one. Results: The day-seven HI score of the wounds applied with cellulose was higher than those applied with zinc oxide non-eugenol. Meanwhile, the WHI of the cellulose group was lower than that of the zinc oxide non-eugenol group, except on day twenty-one. Both the HI and WHI scores then were analysed using Mann Whitney. Conclusion: The application of cellulose is better than zinc oxide non-eugenol on the healing of open wounds after periodontal surgery.

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