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All Journal Jambi Medical Journal "Jurnal Kedokteran dan Kesehatan" MEDIC MEdical Dedication Riset Informasi Kesehatan Journal of Medical Studies
Hanina, Hanina
FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health Other

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Journal : Jambi Medical Journal "Jurnal Kedokteran dan Kesehatan"

 1 
COMPARISON OF THREE METHOD OF DNA EXTRACTION FOR METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Hanina Hanina; Humaryanto Humaryanto
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 10 No. 4 (2022): Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.363 KB) | DOI: 10.22437/jmj.v10i4.21047

Abstract

Background: MRSA is a gram positive bacteria that has a peptidoglycan in the cell wall which is noteasy to lysis during the DNA extraction procces.Objectives: This study aimed to compare three DNA extraction method, using a conventional method, chelex method, and commercial kit in MRSA.Methods: This is a descriptive study with laboratory experiments. The sample was swabs of purulent wounds from inpatients in Jambi that were randomly selected. Swab sample identified as MRSA was extracted with three methods and tested using UV-Vis spectrophotometer.Results: The study results showed that the best DNA purity was found by DNA extraction usingcommercial kit (1.43), followed by chelex (1.14) and conventional (1.07) methods. Likewise, the highest concentration of DNA was DNA extraction using commercial kit (330 g/ml), followed by the chelex (78 g/ml) and conventional (54 g/ml) methods.Conclusion: A conventional method can be used as an extraction method that is easy to do and more economical. The best DNA purity and concentration was obtained in the kit extraction method, followed by chelex and conventional methods.Keywords: MRSA, extraction, conventional, chelex, kit
Dextran Sulfate Sodium Effectiveness As Inflammatory Bowel Disease Inducer In BALB/c Mice Hanina Hanina; Lipinwati Lipinwati; Tia Wida Ekaputri Hz; Hasna Dewi
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 11 No. 4 (2023): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jmj.v11i4.22941

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ABSTRACT Background: Inflamatory Bowel Disease (IBD) in animal model could be induced by chemical agents such as dextran sulfate sodium (DSS),  trinitrobenzene sulfanic acid and oxazolone. The inflammation induced by DSS gave many clinical symptoms and immnulogic reactions like in human. In the recent study, the disease activity index has been assessed on the BALB/c mice that induced by DSS 2% and 3%, the result was no significant result. Therefore, DAI score was not necessarily describe the intestinal tissue real condition, so the researcher want to continue assessing DSS influence to the microscopic features of BALB/c mice intestine and colon. Methods: Nine male BALB/c mice 6-8 weeks, weight 25-40 g divided in 3 groups. Group I as control, while group II and III induced by  2 cycles of 2% DSS for 5 days followed by drinking water for 10 days and 3 cycles of 3% DSS for 7 days followed by drinking water for 7 days. Assesment of DSS effectiveness by microscopic examination of intestine and colon to observe inflammatory features. Results: . The microscopic features of group II and group III mice intestine and colon has no inflammatory features as control group. Oral admission DSS 2% and DSS 3% did not establish microscopic changes in BALB/c mice intestine and colon, so it was not effective as mice IBD inducer.  
The Staphylococcus Aureus Antibiotics Resistance’s pattern in Osteomyelitis Cases at Hospital in Jambi City Lipinwati Husman; Budi Justitia; Hanina Hanina
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 11 No. 4 (2023): JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jmj.v11i4.29020

Abstract

Osteomyelitis is still a health problem in Indonesia, due to poor hygiene, late diagnosis, patients with open fractures who seek treatment late, treats osteomyelitis is long time and quite expensive. Osteomyelitis is an inflammation of the bone that is often caused by a bacterial infection. Osteomyelitis often occurs in open fractures, infections of the feet of diabetics, or surgical treatment of closed wounds. Osteomyelitis can be caused by bacteria, fungi, or other organisms, and is idiopathic. The bacteria that most often causes osteomyelitis is the genus Staphylococcus, which is found in 75% of cases of osteomyelitis, especially Staphylococcus aureus. We wanted to know how the prevalence of osteomyelitis with S. aureus infection was and the pattern of resistance of these bacteria to antibiotics. This research was a descriptive research with laboratory experiments. The research samples were patients with osteomyelitis who had signed informed consent form, samples were taken in the form of wound swabs/bone scrapings and blood using the total sampling method since August 2022 until December 2022. Wound swabs/bone scrapings were performed bacterial culture on Mannitol Salt Agar (MSA). MSA was incubated at 37°C for 18 - 24 hours. The cultures were done gram staining, catalase test and coagulase test. Cultures that grown in MSA with coccus positive gram, catalase test positive and coagulase test positive then tested resistance to antibiotics (Cefoxitin/FOX 30 mg, Ceftriaxone/CRO 30 mg, Gentamicin/CN 10 mg, Chloramphenicol/C 30 mg, and Ciprofloxacin/CIP 5 mg) using the disc diffusion method on Mueller Hinton Agar (MHA) with fresh culture bacteria 0.5 mc Farland. There are 18 samples swabs from wound, and 10 samples are identified as Staphylococcus sp. There are 9 samples that gram staining with coccus positive gram, Catalase positive test, and coagulase positive test. From 9 samples which done antibiotic sensitivity test, there are 2 samples, 3 samples, 1 sample, 3 samples, and 5 samples resistence to cefoxitin (Methicillin resistance Staphyloccus aureus/MRSA), Ceftriaxon, Chlromphenicol, Gentamisin and Ciprofloxacin respectively. There are Fifty percents of osteomyelitis samples are staphylococcal infection with 22.22 percents infection of MRSA.
Co-Authors Aurora Wahyu Indah Dwi Budi Justitia Dini Pasla Ramadhanti Fairuz Fairuz Fenny Merselly Gading Patrick William Gita Mayani Hasna Dewi Humaryanto Humaryanto Humaryanto, Humaryanto Huntari Harahap Iskandar, Mirna Marhami Lipinwati Husman Lipinwati Lipinwati Maria Estela Karolina Natasya Fadia Haya Anindya Hanis Patrick William Gading Putri Sari Wulandari Rina Nofri Enis Rita Halim Tia Wida Ekaputri Wahyu Indah Dewi Aurora