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Saptarini, Nyi M.

Universitas Padjadjaran, Indonesia

Author-ID : 839195

Medicine & Pharmacology

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Inhibitory Activity of Andrographolide and Andrograpanin on the Rate of PGH2 Formation Sumiwi, Sri A.; Halimah, Eli; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Cyclooxygenase (COX) or prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid into prostaglandins. Nonsteroidal anti-inflammatory drugs (NSAIDs) work by inhibiting both COX-1 and COX-2 isoforms, thus disturbing this reaction. In Indonesia, Andrographis paniculata (local name: sambiloto), is empirically used to reduce inflammation by consuming the herb tea of this plant. This work studied the inhibitory activity of andrographolide and andrograpanin, diterpenoids of the plant, on the rate of prostaglandin formation. Previous works have proven that andrographolide inhibited PGE2 production in LPS-induced human fibroblast cells. This study was performed by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by andrographolide and andrograpanin. Acetosal was used as a control drug. The rate of PGH2 formations on either COX-1 or COX- 2 was affected by andrographolide and andrograpanin. Andrographolide and andrograpanin interact longer with COX-1 than COX-2. Andrographolide shows weak inhibition on the rate of PGH2 formation, whilst andrograpanin might be further developed for potential antiinflammatory drugs.Keywords: Andrographis paniculata, anti-inflammatory, COX, cyclooxygenase, prostaglandin

Prediction of Binding Mode of Secondary Metabolites in Apium graveolens to Bcl-2 Shan, Kee P.; Saptarini, Nyi M.; Levita, Jutti
Pharmacology and Clinical Pharmacy Research Vol 2, No 2
Publisher : Universitas Padjadjaran, Indonesia

Developing new cytotoxic agent which has minimal effect against normal cell is requiredto reduce the side effects obtained from the existing chemotherapy agents. Celery (Apiumgraveolens) showed several pharmacology activities, including anti-cancer. This study wasconducted to predict and visualize the binding mode of ten secondary metabolites in A. graveolens,i.e., apigenin, caffeic acid, kaempferol, limonene, shikimic acid, chlorogenic acid,ascorbic acid, quercetin, eugenol and ferulic acid against Bcl-2. Navitoclax was employed asreference. Molecular dokcing simulation was performed using AutoDoc Vina v.1.5.6. Theresults showed that the interaction enery (Ei) ranged from -4.20 to -6.01 kcal/mol, whereasinhibition constant (Ki) were 40.15 to 842.29 Î¼M. Kaempferol showed the best affinity toBcl-2 (Ei=-6.01 kcal/mol; Ki=24,21 Î¼M). Hydrogen bonds were bond between kampferoland Lys20, Ser102, and Arg103, amino acid residues in Bcl-2.In conclusion,Keywords: anti-cancer, Apium graveolens, Bcl-2, navitoclax

Zingiber officinale var. Rubrum Reduces the Rate of Prostaglandin Production Fikri, Fauzan; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 1
Publisher : Universitas Padjadjaran, Indonesia

In Indonesia, red ginger (Zingiber officinale var. Rubrum) is usually used as topical pain reducer by directly applying the fresh rhizome. The aim of this research was to provide information regarding the pharmacological activity of Z. officinale var. Rubrum rhizome infusion on the rate of prostaglandin production. The Z. officinale var. Rubrum rhizome used in this research was purchased from Research Institute for Spices and Medicinal Plants (Balittro) Manoko Lembang, West Java, Indonesia. This research was conducted by applying TMPD (N,N,Nâ,Nâ-tetramethyl-p-phenylendiamine) as the reagent. COX-1 and COX-2 enzyme inhibitory activity can be seen from TMPD chromogenic changes that occur during PGG2 reduction to PGH2. Phytochemical screening showed that flavonoid, quinone, and monoterpenoid/sesquiterpenoid were detected in both dried rhizomes and the water extract. Three spots were detected on thin-layer chromatography system which employing chloroform-methanol (5:5) as the eluent. The rate of prostaglandin formations either by Z. officinale var. Rubrum rhizome infusion or acetylsalicylic acid on COX-1 is slower (at 25th minutes) rather than COX-2 (5th minutes). We concluded that the rhizome of Z. officinale var. Rubrum reduces the rate of prostaglandin production. The rhizome of red ginger reduces the rate of prostaglandin production, which is slower in COX-1 than in COX-2. This plant could be further developed as anti-inflammatory drug candidate.Keywords: acetosal, antiinflammation, cyclooxygenase, NSAIDs, red ginger

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