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All Journal Buletin Penelitian Tanaman Rempah dan Obat PROSIDING SEMINAR KIMIA Biopropal Industri Jurnal Fitofarmaka Indonesia Jurnal Ilmiah Manuntung Jurnal Farmamedika (Pharmamedica Journal) Buletin Penelitian Tanaman Rempah dan Obat
Lilik Sulastri
Sekolah Tinggi Teknologi Industri Dan Farmasi Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Journal : Jurnal Ilmiah Manuntung

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SENYAWA KIMIA STEROL DARI KULTUR JARINGAN KELADI TIKUS (Typhonium divaricatum Decne) Sulastri, Lilik; Simanjuntak, Partomuan
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

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Abstract

Keladi tikus (Typhonium divaricatum Decne) is one of Aracaceae family which a plant that?s very popular in Indonesian society because it contains anti-cancer substances. Nowadays, keladi tikus plants have been cultivated in tissue culture widely. The simplicia used in this study was keladi tikus from tissue culture which was separated into the upper part (leaves and petioles) and the bottom (tubers) dried in the sun. Dry simplicia was extracted with methanol at a temperature of 60-70 oC and the methanol extract was partitioned with ethylacetate then with n-BuOH solvents, and all extracts were tested for cytotoxic tests using the Brine Shrimp Lethality test (BSLT) method. The active fraction of ethylacetate extract, which is the tuber parts was carried out by column chromatography (SiO2; i). n-hexane: ethylacetate = 10: 1 ~ 1: 1; ethylacetate; MeOH; ii). n-hexane-ethylacetate = 5: 1) give one pure isolate. The results of identification based on Infrared (IR) spectra, and gas chromatography-mass spectra (GC-MS) are b-sitosterol which LC50 is 76.2 ppm
Co-Authors . Syamsudin Achmad Fauzi Isa Arini Khaerunnisa Aufa Ali Fajar Nanda Nasukha Danty Nur Alvionitasari Djamil, Ratna Edi Susanto Herson Cahaya Himawan Ika Oktavia Laura Stephanie Joner Lia Rahmania Nico Satria Salim Nuraini Saadah Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Putri Syafalia Ratna Djamil Riska Diana Ristanti Mega Lestari Siti Mariam Syamsoedin Abdilah Syamsudin Syamsudin Verari Vernando Yesi Desmiaty, Yesi