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Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Kenconojati, Hapsari; Rukman, Nina Rofiâ€™
Journal of Aquaculture Science Vol 4, No 1 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9Â± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.

Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Rahayu, Nanik Ning; Prayogo, Prayogo; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method

Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Azizah, Aulia; Budi, Darmawan Setia; Fasya, Arif Habib; Kenconojati, Hapsari; Azhar, Muhammad Hanif
Journal of Aquaculture Science Vol 4, No 2 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions.Â Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus.Â

The Insidensi dan Derajat Infeksi Anisakiasis pada Ikan Hasil Tangkapan di Pelabuhan Perikanan Pantai Muncar, Banyuwangi, Jawa Timur (INCIDENCE AND DEGREE OF ANISAKIASIS INFECTION IN FISH CATCHES AT THE FISHERY PORT OF MUNCAR BEACH, BANYUWANGI, EAST JAVA) Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Muhammad Hanif Azhar; Hapsari Kenconojati
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The purpose of this study was to determine the incidence and degree of infection anisakiasis in fish catches at Fishery Seaport Muncar, Banyuwangi as an effort to prevent food borne diseases in humans. Fish samples from Muncar Beach Fishery Seaport, Banyuwangi consisted of tuna fish (Euthynnus sp.), bloated fish (Rastrelliger sp.), giant trevally fish (Caranx sp.) and snapper (Lutjanus sp.) with total amount 192 tail. Sample fish was dissected and examined the parasite by native method. Parasitic worms was fixed with 5% glycerine alcohol, stained with SemichenAcetic Carmine method, identified by key identification and calculated incidence and degree of infection. The highest incidence and degree of infection showed in Lutjanus sp. and Rastrelliger sp. 66.67% and 5.39, respectively, while the lowest showed in Caranx sp. from 6.67% and 0.09 respectively. Continue research to determine incidence and degree of infection anisakiasis in each season was required to mapping and to know the spread of worms Anisakis sp.

Dominansi dan Diversitas Lamun dan Makrozoobenthos pada Musim Pancaroba di Pantai Bama, Taman Nasional Baluran, Situbondo [Dominance and Diversity of Seagrass and Macrozoobenthos on Transition Season in Bama Beach, Baluran National Park, Situbondo] Sapto Andriyono; Daruti Dinda Nindarwi; Hapsari Kenconojatia; Daruti Dinda Nindarwi; Darmawan Setia Budia; Muhammad Hanif Azhar; Mohammad Faizal Ulkhaq
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 1 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

AbstrakLamun merupakan tanaman berbiji terbuka yang mampu tumbuh dan beradaptasi dengan lingkungan bersalinitas tinggi serta dapat berasosiasi dengan benthos. Keberadaan lamun di perairan merupakan salah satu indikator tingkat kesuburan dan produktivitas perairan. Terdapat perbedaan dominansi antara musim hujan dan musim kemarau, sehingga enelitian ini bertujuan untuk mengidentifikasi dan menghitung dominansi dan keanekaragaman lamun dan makrozoobenthos pada musim pancaroba di Pantai Bama, TN Baluran, Situbondo. Metode penelitian yang digunakan yaitu line transect quadran dengan 5 line transek quadran yang masing-masingnya dipasang 5 plot transek. Ditemukan jenis lamun yang memiliki nilai kelimpahan tertinggi di Pantai Bama, TN Baluran pada musim pancaroba yaitu C. serrulata dengan nilai 48,90% , dan makrozoobenthos dari genus Holothuria dengan nilai 52,06%. Nilai Indeks dominansi (D) mengkategorikan Pantai Bama, TN Baluran dalam perairan dengan dominansi sedang. Sedangkan berdasarkan Indeks keanekaragaman (H’) mengkategorikan Pantai Bama, TN Baluran dalam perairan dengan keanekaragaman rendah. Musim peralihan berpengaruh terhadap jenis, kelimpahan relatif, indeks dominansi dan indeks keanekaragaman lamun dan makrozoobenthos di Pantai Bama, TN Baluran dibandingkan musim kemarau dan hujan. Perlu dilakukan survei secara berkala untuk mengetahui keanekaragaman organisme yang berkaitan dengan produktivitas perairan. AbstractSeagrass is the one of plants that can grow and adaptataion with high salinity environment and associated with benthos. Seagrass presence that indicate the productivity and prosperity in waters environtment. Found a different dominance between rainy season and dry season, so The aims of this study to identify and count dominance and diversity of seagrass and macrozoobenthos on the transition season in Bama Beach, TN Baluran, Situbondo. The method was used is line transect quadran with 5 quadran each of which installed 5 plot transect. Seagrass species was founded that have the highest abundance values in Bama Beach, TN Baluran the transitional season is C. serrulata with a value of 48.90%, and the macrozoobenthos that found from genus Holothuria with a value of 52.06%. Dominance index value (D) categorize Bama Beach, TN Baluran with moderate dominance. While based on the diversity index (H ') categorizes Bama Beach, TN Baluran with low diversity. Transitional seasons affect the type, relative abundance, dominance index and seagrass diversity index and macrozoobenthos in Bama Beach, TN Baluran than the dry and rainy seasons. Needed to investigate the diversity of organism that related to primary produktivity.

Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Hapsari Kenconojati; Nina Rofi' Rukman
Journal of Aquaculture Science Vol 4 No 1 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i1.64

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.

Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Aulia Azizah; Darmawan Setia Budi; Arif Habib Fasya; Hapsari Kenconojati; Muhammad Hanif Azhar
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.71

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus.

Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Nanik Ning Rahayu; Prayogo Prayogo; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method

Microbiological Examination of Escherichia coli and Salmonella on Fishery Products at Fish Quarantine Station, Quality Control and Safety of Fishery Products Yogyakarta Mohammad Faizal Ulkhaq, S.Pi., M.Si; I.A. Nindi; H. Kenconojati; I.N. Putriantini; Inaiyah Inaiyah
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.106

Microorganisms that often contaminate fishery products were from bacteria groups. Some spesies of bacteria were harmful to human health because they can cause disease and even death in humans, such as Escherichia coli and Salmonella. Therefore its were needed for quarantine and microbiological examination of fishery products as an effort to prevent the spread of disease in humans. The aims of this study were to identify the contamination of E. coli and Salmonella bacteria in fishery products. A total eight samples of fishery products tested consist of canned dan frozen products using conventional methods based on ISO 16649-3: 2015 for E. coli and ISO 01-2332.2-2006 for Salmonella. The results showed that one from the six fishery products tested were frozen boiled tuna that positively E. coli and no fishery product sample was identified with Salmonella.

Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region Anggi Rusita Rahmawati; Mohammad Faizal Ulkhaq; Dewi Susanti; Hapsari Kenconojati; Arif Habib Fasya
Journal of Marine and Coastal Science Vol. 10 No. 2 (2021): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v10i2.27658

The freshwater aquaculture fishery sector in Indonesia has the potential to be developed through extensification and intensification. The problem in aquaculture is diseases that infects cultivated organisms, one of which is caused by bacterial infection. Bacterial infection can cause huge losses to cultivation activities, including death which results in economic losses. The purpose of this study is to identify the A. salmonicida and E. ictalury in live fish that will be exported from DI Yogyakarta. The method of examining bacteria used is conventional which consists of isolation, purification, presumptive test, biochemical test, and identification. The results showed that all live fish samples (koi, gouramy, goldfish, catfish, and eel) were not infected with A. salmonicida and E. ictalury. This shows that all live fish are in a healthy condition and suitable to be exported of DI Yogyakarta.

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