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Journal : Journal of Aquaculture Science

Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Kenconojati, Hapsari; Rukman, Nina Rofi’
Journal of Aquaculture Science Vol 4, No 1 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.252 KB) | DOI: 10.31093/joas.v4i1.64

Abstract

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.
Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Rahayu, Nanik Ning; Prayogo, Prayogo; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

Abstract

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method
Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Azizah, Aulia; Budi, Darmawan Setia; Fasya, Arif Habib; Kenconojati, Hapsari; Azhar, Muhammad Hanif
Journal of Aquaculture Science Vol 4, No 2 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.049 KB) | DOI: 10.31093/joas.v4i2.71

Abstract

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus. 
Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Hapsari Kenconojati; Nina Rofi' Rukman
Journal of Aquaculture Science Vol 4 No 1 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i1.64

Abstract

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.
Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Aulia Azizah; Darmawan Setia Budi; Arif Habib Fasya; Hapsari Kenconojati; Muhammad Hanif Azhar
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.71

Abstract

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus. 
Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Nanik Ning Rahayu; Prayogo Prayogo; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

Abstract

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method
Microbiological Examination of Escherichia coli and Salmonella on Fishery Products at Fish Quarantine Station, Quality Control and Safety of Fishery Products Yogyakarta Mohammad Faizal Ulkhaq, S.Pi., M.Si; I.A. Nindi; H. Kenconojati; I.N. Putriantini; Inaiyah Inaiyah
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.106

Abstract

      Microorganisms that often contaminate fishery products were from bacteria groups. Some spesies of bacteria were harmful to human health because they can cause disease and even death in humans, such as Escherichia coli and Salmonella. Therefore its were needed for quarantine and microbiological examination of fishery products as an effort to prevent the spread of disease in humans. The aims of this study were to identify the contamination of E. coli and Salmonella bacteria in fishery products. A total eight samples of fishery products tested consist of canned dan frozen products using conventional methods based on ISO 16649-3: 2015 for E. coli and ISO 01-2332.2-2006 for Salmonella. The results showed that one from the six fishery products tested were frozen boiled tuna that positively E. coli and no fishery product sample was identified with Salmonella.
Microbiological Examination of Escherichia coli and Salmonella on Fishery Products at Fish Quarantine Station, Quality Control and Safety of Fishery Products Yogyakarta Mohammad Faizal Ulkhaq, S.Pi., M.Si; I.A. Nindi; H. Kenconojati; I.N. Putriantini; Inaiyah Inaiyah
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.106

Abstract

      Microorganisms that often contaminate fishery products were from bacteria groups. Some spesies of bacteria were harmful to human health because they can cause disease and even death in humans, such as Escherichia coli and Salmonella. Therefore its were needed for quarantine and microbiological examination of fishery products as an effort to prevent the spread of disease in humans. The aims of this study were to identify the contamination of E. coli and Salmonella bacteria in fishery products. A total eight samples of fishery products tested consist of canned dan frozen products using conventional methods based on ISO 16649-3: 2015 for E. coli and ISO 01-2332.2-2006 for Salmonella. The results showed that one from the six fishery products tested were frozen boiled tuna that positively E. coli and no fishery product sample was identified with Salmonella.