Vol 10, No 3 (2022)

Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle

Tinda Afriani (Universitas Andalas)
Endang Purwati (Universitas Andalas)
James Hellyward (Universitas Andalas)
Jaswandi Jaswandi (Universitas Andalas)
Mangku Mundana (Universitas Andalas)
Anna Farhana (Universitas Andalas)
Adisti Rastosari ((SCOPUS ID: 57208306771) Department of Animal Production Technology, Faculty of Animal Science, Universitas Andalas)

Article Info

Publish Date
31 Dec 2022


The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.

Copyrights © 2022

Journal Info





Earth & Planetary Sciences Other


JURNAL ILMIAH PETERNAKAN TERPADU (JIPT) is a double blind peer-reviewed open-access journal with an editorial board made up of experts in this field. JIPT is a scientific journal covering various aspects of animal husbandry science published since 2012. JIPT is published three times a year in March, ...